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The toxicity and biological activity of aqueous extracts of herbal medicine Naran N were investigated using human skin fibroblast cells culture (HSF) of 2 x 104 and 1 x 10s cells/ml densities. The herbal drag Naran N is composed of four herbs: Flos Calendulae, Folium Plantaginis lanceolatae, Anthodium Chamomillae, Herba Euphrasiae; it was elaborated in the Department of Chemistry, Pharmaceutical Falcuty of Medical University of Lublin (Poland) and distributed on permission ofthe Polish Department of Hygiene (PZH) in 2001. Naran N is produced by Dyspak (Dys near Lublin, Poland) on licence of the Medical University of Lublin (PL-409218-B,). The investigations in the Institute of Microbiology and Biotechnol­ogy of UMCS of Lublin have shown that the aqueous extract of Naran N stimulates the pro­liferation of HSF cells of 1 x TO5 cells/ml density, the viability of cells remained on the con­trol level. Similar viability was observed also for HSF culture of 2 x 10" cells/ml density. The results explain the healing activity of Naran N for chronic vascular diseases.
Lamium album, commonly known as “white dead nettle,” is a perennial herb widely used in folk medicine. The present paper presents the toxic, anti-proliferative, and free radical (DPPH˙) scavenging activities of methanol and ethyl acetate extracts of that plant. In order to determine the biologically active compounds, the plant extracts were separated by high performance thin layer chromatography (HPTLC) on silica gel Si 60 F254 and high performance liquid chromatography (HPLC) combined with densitometry. Methanol extract was rich mainly with flavonoids and phenolic acids. Ethyl acetate extract contained mainly triterpenes. Both extracts showed no toxic effects against normal human skin fibroblasts (HSF) in the range of applied concentrations (25–225 μg/ml). Anti-proliferative activity revealed that methanol extract expressed lower inhibitory properties than ethyl acetate one. The MTT test was, however, less sensitive than Neutral Red (NR) assay. Ethyl acetate extract did not exhibit DPPH˙ radical scavenging activity. Methanol extract reduced the radical of about 29% at the highest applied concentration (225 μg/ml). Both extracts slightly influenced cellular cytoskeleton organization and amount, and size of agyrophilic nucleolar organizer regions (AgNOR) protein deposits. These findings suggest that extracts of Lamium album exhibit potential usefulness in preparation of new natural formulations.
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