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The sensitivity of soil enzymes to soil contamination with zinc was analyzed. A laboratory experiment was performed on sandy loam at pH 7.0, sampled from arable land at a depth of 0 to 20 cm. Soil samples were passed through a sieve with 2 mm mesh size and contaminated with the following zinc doses: 0, 300, 600, 1200 and 2400 mg Zn2+ kg-1 soil. Zinc was applied in the form of aqueous solution of ZnCl2. Soil was mixed thoroughly with zinc, and its moisture content was brought to 50% capillary water capacity. The samples were incubated at 25°C. Beakers with soil samples were weighed once a week to replenish evaporated water. The activity of soil enzymes: dehydrogenases, urease, acid phosphatase, alkaline phosphatase, catalase, arylsulfatase and b-glucosidase, was determined after 15, 30, 60 and 120 days of the experiment. The results were used to calculate soil resistance (RS), ED20 and ED50 values. The results of the study indicate that soil enzymes are characterized by varied sensitivity to excessive zinc concentrations, and that the RS index is a reliable measure of enzymatic responses to zinc pollution. The analyzed enzymes were classified in the following decreasing order in terms of their resistance to zinc: b-glucosidase> acid phosphatase > urease >arylsulfatase = alkaline phosphatase> catalase > dehydrogenases. Zinc continued to exert a negative effect on soil enzymes throughout the experiment (120 days). ED20 values for the analyzed enzymes in mg Zn2+ kg-1 DM soil were determined at: 103 for dehydrogenases, 184 for alkaline phosphatase, 233 for urease, 247 for arylsulfatase, 416 for acid phosphatase, 419 for catalase and 1373 for b-glucosidase.
A field experiment was carried out in 2008−2010 at the Agricultural Experimental Farm of the University of Natural Sciences and Humanities in Siedlce, located in Zawady. The experiment was established on light loamy soil which belonged to a very good rye complex. The experiment was designed in a two-factor split-plot pattern with three replicates. The factors examined in the experiment included three cultivars of edible potatoes: Cekin, Satina, Tajfun, and five weed control methods: application of the following herbicides (mixed or alone) Command 480 EC, Command 480 EC and Dispersive Afalon 450 SC, Stomp 400 SC, Stomp 400 SC and Dispersive Afalon 450 SC, while the control consisted of mechanical weeding without chemical protection. The aim was to determine the influence of selected herbicides and their combinations (Command 480 EC, Command 480 EC and Dispersive Afalon 450 SC, Stomp 400 SC, Stomp 400 SC and Dispersive Afalon 450 SC) on the content of copper, zinc and manganese in three potato cultivars. Potato tubers were harvested at full maturity. Samples of potato tubers for chemical analyses were taken from plots during harvest. Microelements in the dry matter of potato tubers were determined by the AAS method. The content of copper was significantly dependent on the cultivars, weed control methods and weather conditions during the growing season. In contrast, concentrations of depended on the cultivars and meteorological conditions, whereas the content of manganese in potato tubers was strongly conditioned by genetic traits of the cultivars.
Disturbance of the antioxidative-oxidative balance is a predisposing factor in the development of a variety of diseases. Microelements, including zinc and copper, are components of active enzyme regions participating in anti-oxidative systems. Patients with malabsorption syndrome may suffer from diseases related to the chronic deficiency of micro- and macroelements and vitamins, but also experience health problems due to an impaired antioxidative plasma defence, i.e. illnesses caused by oxidative stress. The aim of the study was to assess zinc and copper concentrations as well as the total antioxidant capacity of blood plasma in 33 patients hospitalized in the Department of Internal Medicine, Metabolic Diseases and Dietetics, presenting symptoms of intestinal malabsorption such as weight loss, abdominal pain and diarrhoea. Blood donors made up the control group. Statistically significantly lower concentrations of zinc and copper were found in the study group as compared to the control group for women and men together as well as for women and for men separately (p < 0.05). A significantly lower FRAP (Ferric Reducing Ability of Plasma) value as a measure of blood plasma the total antioxidative capacity was found in the study group of men in comparison with men from the control group (p < 0.05). Patients showed deficiencies of the two microelements despite normal values of BMI (Body Mass Index) and laboratory parameters such as haemoglobin, protein, albumin and iron concentrations. Regardless of the nutritional state assessment, patients with malabsorption syndrome should receive adequate supplementation of vitamins and microelements.
The efficacy of zinc application in the treatment and prevention of diarrhoea in the weaning period of piglets has been demonstrated. However, beside this positive effect some authors suggested the possibility of harmful effects of zinc oxide supplementation on the environment and public health. The aims of the study were to evaluate, in experimental and field conditions, the influence of different doses of ZnO on productivity, losses and body weight gain (b.w.g.) as well as the Zn level in different tissues of pigs. During the first stage of the study three groups of weaned piglets were fed high doses (2 500, 3 000 and 3 500 ppm) of ZnO for 7 days prior to and 14 days after weaning. The best results were observed in the group fed with highest doses of ZnO, in this group daily b.w.g. was 26 grams higher in comparison with the controls. Tissue samples from muscles and selected organs (liver, kidneys, pancreas, heart and brain) were taken from experimental pigs at slaughter after the animals had reached a body weight of about 100kg. The results of toxicological examination indicated that 14-days of treatment with high doses of ZnO have no influence on zinc concentration in tissues. Field experiments aimed at safety and evaluation of the usefulness of ZnO on the prophylaxy of post weaning diarrhoea, performed in 8 farms, demonstrated reduction of diarrhoea symptoms from about 48 to 15%. In summary, it may be concluded that short-term application of ZnO in prevention of post weaning diarrhoea is efficient for pigs and does not influence zinc concentration the fatteners tissues.
Corn was grown for green matter in a pot experiment, in which soil was fertilized with composts alone or in combination with 3 g N per pot (urea). The composts were made in wooden boxes, measuring 50×60×60 cm. They were composted for 3.5 months, until the temperature in the composts became stable and equal to the ambient temperature. Rates of the composts were balanced with amounts of added nitroge, such as 6.0 g N per pot. A one-factor experiment was conducted in Kick-Brauckmann pots, kept in a greenhouse at the University of Warmia and Mazury in Olsztyn. After harvest, fresh matter of stems and leaves as well as corn cobs was determined. Next, the plant samples were dried to determine the dry matter content and finally, after mineralisation, the concentration of Cu, Zn and Mn was determined by atomic absorption spectrophotometry. The composts significantly reduced the concentration of zinc and manganese in the vegetative yield of cor (lstems and leaves). Urea applied in combination with the composts very strongly increased the concentration of manganese and, to a lesser degree, the level of zinc and copper in vegetative organs. In corn cobs, the application of urea increased only the content of manganese. Urea had a stronger effect on increasing the weight of cobs rather than the vegetative mass of corn plants. The uptake of Cu, Zn and Mn was more evidently conditioned by the accumulation of these elements in dry matter than by the total weight of corn plants. The extent of the relationship between the uptake of Cu, Zn and Mn and their concentration of corn biomass is expressed the by corresponding correlation coefficients: 0.66, 0.65 and 0.68.
In the paper, the effects of differences in land use of a catchment and settlements on the variability of heavy metals concentrations (Zn, Pb, Cu, Cd, Ni) in the Narew River and some of its tributaries such as the Biebrza, upper Narew, Pisa, Omulew and Rozoga rivers were studied. The investigations continued from 1997 to 2002. The areas dewatered by the watercources differ considerably in terms of environmental features (forests, wetlands, bogs and water reservoirs), land use (arable lands, grasslands) and population (man-made areas). The total catchment of the Narew River at the cross-section in Zamski Kościelne was also taken into account. The urban areas make up from 0.5% of the Rozoga River to 2.5% of the upper Narew catchment. Water samples were collected quarterly (April, July, October and January) from four study sites located near the river mouth and were analyzed for Zn, Pb, Cu, Cd and Ni by the AAS method. The results showed that concentrations of Pb, Cu, Cd and Ni increased along the Narew River due to the accumulation of the elements in water. A significant influence on high heavy metal concentrations in water was produced by the town of Białystok, a source of large amounts of municipal pollutants discharged into the river. This mainly affected the content of Zn, as the highest pollution with this element was detected in the upper Narew River. The lowest Zn concentrations were observed in the Omulew River water, where forests dominated in the area, covering more than 48% of the catchment. The research showed that wetlands and lakes significantly decreased Pb, Cu, Cd and Ni concentrations in flowing water, but the presence of large inhabited areas with dense population and municipal pollution increased concentrations of heavy metals in river water.
The Premenstrual Syndrome (PMS) is described as a cyclical disorder related to the hormonal changes during a menstrual cycle, which affects the emotional and physical health of many women during their reproductive period of life. The PMS can obviously change the quality of life. The syndrome is characterized by a complex group of symptoms, such as depression, irritability, mood swings, anxiety, abdominal discomfort. These signs occur during the luteal phase of a menstrual cycle and disappear after the onset of menses. Some studies suggest that a variety of nutrients may play an important role in the mood swings which occur cyclically during the course of the premenstrual syndrome and that some can have a beneficial impact, especially on the estrous phase of a menstrual cycle. The aim of the paper is to review the results of some studies concerning the role of bioelements in patients with the PMS. Concentrations of magnesium, zinc, selenium and manganese are the highest during menses and the lowest in the ovulatory phase. Fluctuations of the magnesium concentration during a menstrual cycle and the involvement of this element in many cellular pathways and neuromuscular activities obviously affect the incidence or intensity of the PMS symptoms. However, we lack firm evidence that magnesium supplementation can have a positive effect on alleviating the the PMSrelated ailments. Some relationship between the PMS and bone demineralization or depressed calcium concentration in blood has been identified. However, further studies are necessary to examine how the calcium concentration in a human body can decrease the intensity of the PMS symptoms.
The aim of the study was to evaluate the Fe, Zn and Cu concentration changes in the course of endotoxin tolerance in horses. The study involved 8 clinically healthy mares subjected to 4 iv. injections with 0.1 μg/kg b.w. of LPS from E.coli - three times at 24 hour intervals (LPS-1, LPS-2, LPS-3) and one injection a week after the last injection (LPS-4). Rectal temperature measurements and blood sampling for testing were performed before each LPS administration as well as 1h, 2h, 3h, 5h and 7h thereafter. It has been found that administration of a relatively small dose of endotoxin produced not only a rise in body temperature but also a significant decrease in Fe and Zn concentration in serum. Subsequent injections (LPS-2 and LPS-3) did not result in significant changes of concentration of these elements, which suggests that endotoxin tolerance mechanisms had been switched on. It has been demonstrated that this process does not last longer than one week because LPS-4 administration resulted in renewed decrease of Fe and Zn concentrations. However, no significant influence of endotoxin injection on Cu content in serum was found.
The objective of this study was to investigate the serum concentrations of iron, manganese, cobalt, copper, zinc, and copper/zinc ratio in Downer cows. The study was performed on 20 recumbent Downer cows and 20 healthy cows. The serum concentrations of iron (P=0.002) and copper (P=0.035) were lower in Downer cows compared with the healthy cows. Serum zinc concentrations, although not significantly (P=0.162), were also lower in Downer cows compared with the healthy cows. Cows with Downer syndrome had greater concentrations of serum AST (P<0.001), LDH (P=0.003), and CK (P<0.001). Other biochemical parameters remained unchanged. RBC (P=0.026), Hb (P=0.029), and MCV (P<0.001) in cows with Downer syndrome had lower values compared with those of healthy ones. However, WBC in cows with Downer syndrome had greater values (P<0.00l) compared with those of healthy ones. Other haematological parameters remained similar between Downer cows and healthy cows. It was concluded that serum iron and copper concentrations may play an important role for the aetiology and/or pathogenesis of Downer cows. These elements may be supplemented for the prevention and therapy of recumbency in cows.
The effect of intensive irrigation with wastewater on the zinc (Zn) and cadmium (Cd) concentration in soil solution was investigated. The experiment was parallel conducted on two soil profiles. The soil microcosms were watered with purified wastewater and purified wastewater with an the addition of zinc and cadmium. The results indicate clearly that neither intensive overhead irrigation of the soil nor its flooding with these wastewater and exceeded sorptive capacity. The application of treated wastewater and wastewater with heavy metals addition did not appear to pose a threat to the natural environment. In all the cases under analysis, zinc and cadmium concentrations in the soil solution were several-fold lower than the permissible levels.
Celem pracy było ustalenie, które z badanych roślin (topola, wierzba wiciowa, rzepak, konopie i mieszanka traw) kumulują największe ilości cynku oraz jak zróżnicowany stopień nawadniania gleby wpływa na pobranie tego pierwiastka. Eksperyment przeprowadzono na polach doświadczalnych (gleba torfowo-murszowa) irygowanych ściekami miejskimi o II stopniu oczyszczenia. Pola doświadczalne były podzielone na trzy kwatery: kontrolną (A), zalewaną pojedynczą dawką, ścieków miejskich po II stopniu oczyszczenia (B) oraz zalewaną dawką podwójną (C), Ilość ścieków wprowadzanych na pola wynosiła odpowiednio 600 i 1200 mm w ciągu roku. Oznaczenia stężeń cynku w materiale roślinnym wykonywano metodą absorpcyjnej spektrometrii atomowej (AAS). Stężenia cynku mieściły się w zakresie 11-290 mg kg '. Najwyższą zawartość cynku stwierdzono w liściach topoli, 32-290 mg kg-1. Pozostałe rośliny można uszeregować następująco; wierzba, konopie, rzepak, mieszanka traw.
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