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Special attention in this review is given to published work on genetic, antigenic and pathogenic variations of the virus of porcine reproductive and respiratory syndrome (PRRS) and the potential implications of these properties for vaccine efficacy. Genomic organization of type 1 and type 2 strains of the PRRS virus is presented: the former being the representative of the European strains and the latter of the North American isolates. Among the European isolates 4 subtypes are differentiated. More homogenous are the strains of the Northern American type. Despite very high differences in antigenic properties of both European and North American isolates, it is possible to differentiate them, using serological methods, particularly the immunofluorescence test. The ELISA test proved to be useful in diagnosis of PRRS. However, antigens of the PRRS virus are of no use in the selection of strains for vaccine production. The high degree of variation among PRRS virus isolates is also demonstrated in pathogenic properties. Generally, the heterogeneity of genetic, antigenic and pathogenic properties, being a continuous process, is the explanation for the persistent infections resulting from the selection of mutants that escape neutralizing antibodies or cytotoxic T lymphocytes. Because of the heterogeneity of the mentioned biological properties of the field strains, the immunoprophylactic value of the available vaccines is not high. Better results are obtained when live, attenuated vaccines are used. In relation to this topic the proposition of Mengeling, called SWINE, is presented. The first letter means sensitization of the pig by vaccine injection; the second letter means waiting for more than several weeks until the level of neutralizing antibodies reaches low values; the letter I means immunization; N means neutralization of the virus; the letter E means eradication, in other words the elimination of PRRS. The last step must be supported by biosecurity and additional veterinary measures. Another method of immunoprophylaxis is the vaccination of pigs introduced to a herd using viremic serum of pigs of the same herd, which provides the best homogeneity of immunity and the highest efficacy.
In June 1998, 12 gilts were purchased at a new, small (annual production 250 piglets) farm. The animals were free from PRRSV antibodies. After one month of quarantine the gilts were placed in two separate pens - A and B. The first female was bred in mid-August and the last one at the beginning of October. In order to ensure the empty rooms at the nursery house were occupied, in mid-November the farmer purchased 120,8- -week old weaners from an epidemilogically unrecognised herd. Unfortunately, the weaners were immediately introduced onto the farm. One-week later antibodies to PRRSV were accidentally detected in this group of pigs. Because the pig holding was under the control of the Institute, there was an opportunity to monitor the eventual spreading of PRRSV from weaners to gilts. In order to do this, blood samples were taken from all gilts 2,4,6 and 10 weeks after the weaners had been introduced onto the farm. Four weeks after purchasing the weaners, antibodies were detected in 3 out of 6 gilts from pen A and 2 weeks later PRRSV antibodies were found in a further 2 females. The last gilt from this pen was sero-converted 10 weeks after the weaners had been introduced. In pen B, PRRSV sero-conversion was detected in the first 2 gilts 6 weeks after the weaners introduction, and 2 other gilts were sero-converted 4 weeks later. One gilt from this pen did not have antibodies at the end of the observation period. The consequences of PRRSV spreading among pregnant gilts were observed during the furrowing period. Ten out of twelve females delivered dead or mummified piglets. Three litters were normal and in these cases the gilts had been infected during the 8-9 weeks of pregnancy. The economical consequences of introducing weaners infected with PRRSV into the newly established herd were very serious: the average number of piglets born alive per gilt was 6.8. The average number of piglets weaned was 6.2 and fatteners sold per sow were 5.7.
The epidemiology of the PRRS is much more complicated in comparison to other viral diseases, relating to its spread and persistence of the virus, which is a marked characteristic. For this reason the immunological status was evaluated in different groups of animals. Herds were located in different regions of Poland, with large, medium and small populations of pigs. The evaluation of the titres of antibodies against-PRRS virus were carried out in 48 herds located in selected regions of Poland (in 2 voivodships from regions of large pig populations, in 4 voivodships from regions of medium and in 4 voivodships from regions of small pig populations). A total of 1515 pigs were submitted for the study. In regions of large pig populations 26 herds were tested. In 19 herds (73%) antibodies of anti-PRRS virus were detected. In 3 herds (11%) a stable inactive immunological status was indicated and in 16 herds (62%) an unstable immunological status was indicated. In 7 herds (27%) antibodies of anti-PRRS virus were not observed. In regions of medium pig population 11 herds were tested. In 6 herds (73%) antibodies of anti-PRRS virus were detected. In 1 herd (10%) a stable inactive immunological status was indicated and in 5 herds (45%) an unstable immunological status was indicated. In 5 herds (45%) antibodies of anti-PRRS virus were not observed. In regions of small pig populations 11 herds were also tested (1 herd was not classified). In 5 herds (50%) antibodies of anti-PRRS virus were detected. In 1 herd (10%) a stable inactive immunological status was indicated and in 4 herds (40%) an unstable immunological status was indicated. In regions of small pig populations there was the highest percentage (50%) of herds that were free from the PRRS virus (5 herds).
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