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The peculiarities of heavy metals accumulation by wild medicinal and fruit plants. The article shows the results of studies of heavy metals accumulation by wild medicinal and fruit plants. Special attention is given to the investigation of roadside phytocenoses. Reliable differences in chemical composition of plants from background and transformed habitats are revealed. The influence of morphological and phenological characteristics of the species on the peculiarities of heavy metals accumulation is also determined.
The work undertakes the issue of Zn, Cu, Pb, Cd, Ni and Cr accumulation in wild grown plants in the context of their possible use for the sanitation of sludge and waste substrates. The highest contents of heavy metals were noted in Lactuca serriola, Chenopodium album, Artemisia vulgaris and Atriplex nitens. Assuming maximum crop production which is to be obtained from sludge and waste substrates at a level of 2 kg d.w./m2, it is clear that from 1 hectare several hundred grams of Pb and Cd, as well as up to 2 kg of Cu and 20 kg of Zn may be removed.
Despite the extensive bio-scientific literature concerning the Mediterranean diet, which emerged in the last three decades, systematic ethnography-centered investigations on a crucial portion of this food system, linked to the traditional consumption of non-cultivated vegetables, are still largely lacking in many areas of the Mediterranean Basin. In this research, an ethnobotanical field study focusing on wild vegetables traditionally gathered and consumed locally, was conducted in a few centers and villages located in the Gargano area, northern Apulia, SE Italy, by interviewing twenty-five elderly informants. The folk culinary uses of seventy-nine botanical taxa of wild vascular plants, belonging to nineteen families, were recorded, thus showing a remarkable resilience of traditional environmental knowledge (TEK) related to wild food plants. In particular, approximately one-fourth of the recorded wild vegetables are still very commonly gathered and consumed nowadays, while ten taxa have never been reported in previous ethnobotanical studies conducted in Southern Italy. These findings demonstrate the crucial cultural role played by folk cuisines in preserving TEK, despite significant socio-economic changes that have affected the study area during the past four decades.
In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC–). For each gene the PTC+/PTC– ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC– splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC– ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
Cichorium intybus is edible, medicinal and forage plant. The pharmaceutical raw materials were obtained from wild chicory (var. silvestre). Currently, farmers are increasingly assume plantations of wild chicory, and breeders are attempting to produce cultivars for medicinal purposes. In the modern breeding of chicory important feature is the ability to clonal propagation in vitro culture. The aim of our study was to assess capacity of natural population of wild chicory for plant regeneration from leaf explants. In the first was examined the effect of 16 combinations of various concentrations of IAA and 2iP on the regeneration of shoots from leaf explants (0.5 cm2 ). After that, 25 plants were propagated on the medium which was found as optimal. Then, their callus growth and shoots regeneration capacities were compared. Later on, was examined the effect of various IAA concentrations on the rooting of shoots. The majority of the shoots was regenerated from callus but direct organogenesis was also observed (8%). Shoot regeneration was found to be the most efficient on MS medium containing 0.5 mg dm-3 IAA and 4 mg dm-3 2iP – 97% of the explants produced shoots, while the average number of shoots was 15.5. The amount of callus was found to be a highly heritable trait (h2 = 0.83). Lower the heritability coefficients were obtained for the number of shoots per explant (0.55) and the average shoot weight (0.40). The wild chicory shoots rooted easily. The number and weight of roots increased with the increasing concentration of IAA.
This study examined the potential of liquid wild dog rose (Rosa canina) seeds oil and solid seeds residue obtained after CO2 extraction in supercritical conditions to mitigate rumen methane production in vitro. Two experiments were carried out. The substrate comprised of a mixture of meadow hay and barley meal (60:40) for the control diets (CON1 in experiment with oil and CON2 in experiment with residue). The control diets were supplemented up to 5% in dry matter of rose seeds oil (RO) and 5% of rose seeds residue (RR). The following parameters were measured: pH, ammonia, volatile fatty acids, ciliate protozoa and bacteria count, methane concentration and methanogens population. In the RO treatment a decrease in methane production and an increase in the Archea population were observed. In the RR treatment no change in methane production was reported, whereas some variations in protozoal populations were detected in relation to CON2. The potential to mitigate methane production was reported only in wild dog rose seeds oil treatment. Besides, no negative effect of wild dog rose seeds residue on rumen processes was stated, what may predispose this protein and fibre containing by-product to be utilized as ruminants dietary ingredient.
Wild raspberry (Rubus idaeus L.) is abundantly grown and widely distributed in temperate regions of lower Himalayan range which represents rich biological diversity for raspberry. Thirty accessions were explored from three different locations of Azad Jammu and Kashmir (Pakistan), several traits were visualized at sampling locations and some characteristics were evaluated and quantified after harvest of leaf and fruit samples. The accessions differed greatly in growth habit, blooming time, intensity of flowering, fruit setting, ripening time, productivity, fruit shape and color. Concerning quantitative characteristics, significant differences were found in plant height, leaf length and width, number of braches per plant, duration of flowering, fruit setting percentage, days to maturity, fruit length, width and weight among the samples collected from different locations. Ripe fruits were evaluated for their chemical composition i.e. moisture, carbohydrates, protein, fat, fiber, ash, TSS, acidity, pH and mineral (K, Ca, Na, Fe, Cu, Zn, Mn, Pb, and Cr) contents. The location also had significant effect on chemical composition of ripe fruits.
Magnolia cylindrica Wils. is one of the third most-protected wild plants in China. To describe the size structure and dynamics of its population, field data were obtained from eight newly established sites, using a contiguous grid quadrate method in Jiulong Mountain of East China. The population size structure and spatial distribution pattern were discussed based on a theoretical distribution model and assembling intensity index. The population size structure showed a declining trend because of the lack of seedlings. The number of stump-sprouting, size class III (sapling trees) individuals was large enough to make up for the shortage of small seedlings and the complete regeneration of populations through sprouting. The distributions of M. cylindrica, both seedling populations (Group A) and overall populations (Group B), were mostly clumped. The spatial pattern intensities of the populations at different stages (mainly small trees, middle trees, and big trees) were higher for Group A than those for Group B. The two groups have the same tendency in that the pattern intensity declined from small trees to the larger ones. Group A and Group B differed in spatial pattern: small and middle trees were randomly distributed in seedling populations, but aggregated in overall populations. The populations of M. cylindrica (both group A and B) were characterized by the pattern scale between 16 to 32 m2, measured by Greig-Smith and Kershaw methods. These results suggest that sprouting should be seriously considered in population rehabilitation and forest tending management and the area of forest tending management should be close to the maximum intensity.
Croatia is a country of diverse plant use traditions, which are still insufficiently documented. The aim of this study was to document local traditions of using wild food plants around Lake Vrana (northern Dalmatia, Zadar region). We interviewed 43 inhabitants of six traditional villages north of Lake Vrana. On average 12 species were listed, which in total produced an inventory of 55 food plants and 3 fungi taxa. Wild vegetables were most widely collected, particularly by older women who gathered the plants mainly when herding their flocks of sheep. Wild fruits and mushrooms were rarely collected. The former used to be an important supplementary food for children, or for everyone during times of food shortage, and the latter were relatively rare due to the dry climate and shortage of woods. The most commonly collected plants are wild vegetables: Cichorium intybus, Foeniculum vulgare, Sonchus oleraceus, Asparagus acutifolius, Papaver rhoeas, Rumex pulcher, Daucus carota, Allium ampeloprasum and Silene latifolia.
The content and composition of sterol compounds in wild growing great burnet (Sanguisorba officinalis L.) populations were determined. The herb and underground organs of this plant were collected from 10 natural sites in Poland. The HPLC analysis revealed the presence of 5 sterol compounds, namely β-sitosterol, β-sitosterol D-glucoside, campesterol, brassicasterol and stigmasterol in these raw materials. Among them, β-sitosterol Dglucoside was dominant one. The herb was richer in sterol compounds than underground organs. There were significant differences between the content of above mentioned compounds in these populations.
This study was conducted to determine the mineral contents and some nutritional properties of Trachystemon orientalis L. growing in the Central Black Sea Region, Turkey, and that is consumed as vegetable. Surveys and field studies were started during the spring semester of 2011 (April–May) and they were collected from Samsun and Ordu, where they are intense. In the study, it is was observed that pH, dry matter, content of ash, N, protein, vitamin C, macro- and microelements examined in Trachystemon orientalis varied considerably. The pH, dry matter, ash, N, protein and C content of the plants ranged from 6.61 to 6.88, 13.0 to 22.1%, 9.2 to 17.0%, 2.3 to 3.3%, 14.1 to 20.3 % and 0.12 to 39.03 mg/100g, respectively. Mineral analysis showed that Trachystemon orientalis contained considerably high amounts of potassium (3883.8 to 5791.4 mg/100g), phosphorus (339.7 to 540.9 mg/100 g), calcium (159.4 to 432.4 mg/100g), magnesium (108.0 to 176.4 mg/100 g), iron (10.7 to 63.1 mg/100 g), sodium (22.1 to 66.3 mg/100g), copper (0.6 to 1.5 mg/100 g), manganese (1.5 to 3.6 mg/100g) and zinc (2.3 to 7.6 mg/100g). Mineral compositions of the plants varied significantly depending on the genotypes. T. orientalis was determined as abundant in contents of vitamin C, minerals and protein.
In Tunisia, Thymus capitatus L. populations are severely destroyed due to deforestation and over-collecting. The species occurs in small scattered populations decreasing progressively in size. Yet, no conservation or improvement programs are attempted to preserve and promote the potential value of this resource. In this work, we assessed the genetic diversity of nine Tunisian populations of Thymus capitatus L. from different bioclimates, using 103 polymorphic randomly amplified polymorphic DNA (RAPD) loci. The analysis of the genetic variation within and among populations is primordial to elaborate conservation and improvement programs. The species showed a low diversity within populations (0.276
Wild food and fungi use in the countryside has always been an important part of human-nature relationships. Due to social changes in most rural areas of Europe this part of traditional ecological knowledge is shrinking. The aim of our study was to record the use of wild foods and plants among the Ukrainian (Carpatho-Rusyns) minority in the western part of Romanian Maramureę. We carried out 64 interviews in two villages. Voucher specimens were collected and DNA barcoding was used to identify most fungi taxa. We recorded the use of 44 taxa of plants altogether (including 8 taxa used only for herbal teas) and 24 taxa of fungi. On average 7.7 species of plants and 9.7 species of fungi were listed per interview. Edible fungi are thus an important part of local cuisine and they are eagerly gathered by everyone. The use of a few woodland bracket fungi is worth pointing out. No signs of degeneration of this knowledge were observed. Wild fruits are less collected now and wild greens are rarely collected nowadays. This pattern is typical of many places in Central Europe.
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