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Role of silicon in plant resistance to water stress

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Agricultural productivity is strongly affected by different abiotic stresses, among which water stress is the major environmental constraint limiting plants growth. The primary reason for water stress is drought or high salt concentration in soil (salinity). Because both of these stress factors lead to numerous physiological and biochemical changes in plants and result in serious loss in yields, there is a pressing need for finding the effective ways for increasing crops’ resistance to stress factors. One of the alternative methods involving alleviation of negative stress effects might be application of silicon as a fertiliser (root or foliar supply). Many plants, particularly monocotyledonous species, contain large amounts of Si (up to 10% of dry mass). In spite of the high Si accumulation in plants (its amount may equal concentration of macronutrients), until now it has not been considered as an essential element for higher plants. Many reports have shown that silicon may play a very important role in increasing plant resistance to noxious environmental factors. Hence, Si is recognised as a beneficial element for plants growing under biotic and abiotic stresses. The main form of Si which is available and easily taken up by plants is monosilicic acid (H4SiO4). Plants take up Si from soil solution both passively and actively. Some dicotyledonous plants such as legumes tend to exclude Si from tissues – rejective uptake. These plants are unable to accumulate Si and they do not benefit from silicon. Under water stress conditions, silicon might enhance plants’ resistance to stress and ameliorate growth of plants. These beneficial effects may result from better and more efficient osomoregulation, improved plant water status, reduction in water loss by transpiration, maintenance of adequate supply of essential nutrients, restriction in toxic ions uptake and efficient functioning of antioxidative mechanisms. Based on the current knowledge and presented data, it can be concluded that the role of Si in plants is not restricted to formation of physical or mechanical barrier (as precipitated amorphous silica) in cell walls, lumens and intercellular voids. Silicon can also modulate plants’ metabolism and alter physiological activities, particularly in plants subjected to stress conditions. However, in some plants, increased silicon supply does not improve plant growth. Hence, a better understanding of the interactions between silicon application and plant responses will contribute to more efficient fertiliser practices, especially under stress conditions.
A survey of the capacity to endure desiccation was obtained for several Brazilian rock outcrop plants. Seedlings, leaves and entire plants were subjected to dehydration. After dehydration, survival was observed as the ability to recover growth, chlorophyll content and/or by the absence of leakage of leaft contents. A total of 30 species growing in rock outcroppings in southeastern Brazil showed some ability to undergo extreme dehydration. The species belong to the cryptogams Polypodiaceae, Pteridaceae, Schizaeaceae and Selaginellaceae and to the angiosperms Cyperaceae, Poaceae and Velloziaceae. All cryptogams were homeochlorophyllous. Among the angiosperms Microchloa indica (Poaceae) was the only species with the capacity to retain chlorophyll content during dehydration. All species showed some evidence of desiccation tolerance similar to that previously reported for tolerant angiosperms in Africa. The data suggest that rock outcroppings from the southeastern and central regions of Brazil give support to typical vegetation in which a great number of species exhibit a desiccation-tolerant comportment.
Water stress and provenance could affect the secondary metabolites synthesis and accumulation in herbs. Thus, this study explored the effect of soil water moisture and provenance on the growth of Paris polyphylla Smith var. yunnanensis (PPY). Three provenances (Jinping, Luquan and Weixi in Yunnan, China) of PPY samples were grown in different soil water moisture conditions [0.80, 0.70 and 0.50 field capacity (FC)] during Dec. 2015 to Sep. 2017. Results showed that the highest biomass weight was presented in 0.70 FC for Luquan and Weixi samples. Biomass weight for Jinping provenance presented a decreasing tendency with the decreased soil water moisture and the highest biomass were shown in 0.80 FC. However, quantitative analysis revealed that the total content of polyphyllin increased with decreasing the soil water moisture for Jinping and Weixi samples. The highest total content of polyphyllin in rhizome was inclined to show in Jinping samples, while the stem and leaf tissues were shown in Weixi samples. Additionally, results of ANOVA combined with PCA indicated that the difference among these three provenances were significant. Correlation analysis results revealed that 0.50 FC induced the competitive relationship occurrence for polyphyllin distribution. Thus, 0.70 FC was the most suitable soil-water condition for PPY growth. Besides, provenance collected from Jinping could consider as a good quality germplasm. Consequently, this study might provide a preliminary foundation for irrigation project formulated and provenance screened for PPY cultivation.
The aim of this work was to examine the ability of ABA and proline to counteract the deleterious effect of water deficit stress on cell membrane injuries. Six-day-old seedlings of two barley genotypes (cv. Aramir, line R567) were treated with ABA (2·10⁻⁴ M) or proline (0.1 M) for 24 h, and then subjected to osmotic stress for 24h, by immersing their roots in polyethylene glycol (PEG 6000) solution of osmotic potential of −1.0 MPa and −1.5 MPa or by submerging the leaf pieces in PEG solution of osmotic potential of −1.6 MPa. Pretreatment of plants with ABA and proline caused an increase of free proline level in the leaves. Plants treated with ABA exhibited a lower membrane injury index under water stress conditions than those untreated even when no effect of this hormone on RWC in the leaves of stressed plants was observed. Pretreatment of plants with proline prevented to some extent membrane damage in leaves of the stressed seedlings, but only in the case when stress was imposed to roots. Improvement in water status of leaves was also observed in seedlings pretreatment with proline. The protective effect of both ABA and proline was more pronounced in line R567 that exhibited higher membrane injury under water deficit stress conditions.
The effects of drought stress on changes in the relative water content, in levels of photochemical efficiency and chlorophyll content and in activities of antioxidative enzymes of second leaves of winter wheat (Triticum aestivum L.) cultivars Mironovská and Estica were compared. In one set, control plants were regularly watered, the other set of plant was subjected to water deficit period since 11th day to 18th day of plants life-span. The activity of all antioxidative enzymes and content of β-carotene increased, relative water content, total chlorophyll content and photochemical efficiency decreased after treatment in both cultivars. In the case of Estica the values of antioxidative enzymes activity was higher than in the case of Mironovská. It is therefore possible to assume that Estika is capable of dealing with higher levels of active oxygen species created during drought stress.This might enabled it compensate the effects of drought stres more effectively.
The purpose of the present study was to determine whether water deficit affects cold hardiness of rhododendron ‘Catawbiense Boursault’, ‘Lee’s Dark Purple’, ‘Prinz Karneval’ and ‘Old Port’ shrubs. Plants were grown in unheated greenhouse and for 14 weeks from June to mid-September were subjected to six irrigation treatments. In the end of September shrubs were left in an unheated greenhouse or planted into the ground and at the beginning of each month from December to March freezing tolerance tests were performed. The results showed that in all rhododendron cultivars the highest cold hardiness was noted in January and February, lower in March but the lowest in December. Application of four-week water deficit period during summer especially between the first and the second vegetative growth may improve the frost resistance of Rhododendron shrubs.
The impact of water stress was analysed in the xero-halophyte Mediterranean shrub Atriplex halimus using two Tunisian populations originating from a subhumid coastal site (Monastir) or from a semi-arid area (Kairouan). Seedlings were exposed for 10 days to nutrient solution containing either 0 or 15% polyethylene glycol. Water potential (Ψw), osmotic potential (Ψs), osmotic potential at full turgor [Ψs(100)], relative water content (RWC), shoot dry weight (DW) and changes in solute concentrations were quantified every 2 days throughout the stress period and inorganic solutes contents were determined at the end of the treatment. The water deficit induced a decrease in Ψw, Ψs and RWC in both populations, recorded changes being higher in plants of Monastir than those of Kairouan while the shoot dry weight was reduced in a similar extent in stressed plants from both populations. Water deficit induced an increase in proline, glycinebetaine and sugar concentrations. Proline accumulated as early as after the 24-h stress treatment while, glycinebetaine required more than 6 days of stress to accumulate. At the end of the stress period, the plants of Kairouan population accumulated higher amounts of proline than those of Monastir, while an opposite trend was reported for glycinebetaine. Both populations specifically accumulated Na⁺ in response to drought stress, suggesting that this element could play a physiological role in the stress response of this xero-halophyte species. Presented results suggest that the non-recyclable osmotic solute glycinebetaine does not necessarily preferentially accumulates in population facing permanent water stress and that other strategy than osmotic adjustment might be involved in drought tolerance of A. halimus.
This study presents the data concerning the intensity of lipid peroxidation in seedlings of Arabidopsis thaliana, which were grown under the prolonged water stress on the agarized sterile medium. Growth parameters, such as length of the main root and fresh matter were measured. The seedlings of A. thaliana subjected to water deficit exhibited a decrease in the growth of the main root and fresh weight in comparison to the control plants. Since peroxidation processes due to the concentration of malonic dialdehyde and the level of chemiluminescence of plant homogenate decreased at the end of the experiment, it was concluded that plants were able to adapt to water stress conditions.
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