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The following toxic compounds were administered to the biotest with the application of the protozoan Spirostomum ambiguum as the test object: sodium cyanide, isopropylic methanophosphoric acid ester fluoride (sarin) and phosphoric acid 0-ethylo n-dimethyloamid ester cyanide (tabun). For the purpose of the LCS0 calculations, i.e. the concentrations of the sodium cyanide, sarin and tabun causing death in 50% of the tested Spirostomum ambiguum after an established period of test time, the Münch Reed method as well as as the B-splines, cubic splines and the Bezier curves were used. Calculations have also been made with regard to the average LCS0 concentrations of the analyzed compounds, the standard deviations and confidence intervals. The behavior of Spirostomum ambiguum was observed, establishing the time of death of two organisms. The measurement was carried out 21 times for each concentration and compound in three measuring cycles. On the basis of the data base from the biotest, the following have been proposed: the established LCso values, standard deviations and confidence intervals, the identification of an unknown toxic compound existing in water and determining the concentration of this compound. It has been concluded that Spirostomum ambiguum satisfies the requirements imposed on test animals for the purpose of bioanalysis of sodium cyanide, sarin and tabun.
Water pollution is a matter of great concern in lentic and lotic ecosystems. Generally water pollutants like heavy metals, organic compounds, etc. generate from industries and domestic activities. All of these pose serious threat to the fish population in freshwater as well as marine water bodies. An impact caused by water pollutants especially genotoxins depend not only upon its concentration, but also on the duration (acute and chronic exposure) and showed individual and/or combination of pollutants or genotoxins. Genotoxicity of fish with special reference to micronucleus induction in the erythrocytes of fish is an easy screening of water pollution. The present review deals with past and present research works of major water pollutants discharge and their impact on several fish species from available literatures.
The aim of this research is to assess the water quality of the Liangtang River. Water samples were collected from seven sampling stations and four heavy metals (copper, lead, zinc, and chromium) were determined by spectroscopic technique. Physical and chemical parameters, including pH, DO, NH+4, TN, TP, and COD also were determined from seven water sampling stations sited along the river. It has been found that chemical oxygen demand (CODcr), ammonia nitrogen (NH+4-N), total nitrogen (TN), and total phosphorus (TP) in upstream sites were lower than those of the downstream sites, indicating pollutants being discharged along its course. Analyses also have shown that CODcr, NH+4-N, TN, and TP have a very significant positive correlation between each other, while NH+4-N, TN, and TP have a negative correlation with DO. The major sources of pollution in Liangtan River are urban wastewaters, wastewaters from industries, and other anthropogenic activities along the river. We proposed the strategies that can be applied for pollution reduction.
The present study was performed for the period of one year from January 2013 to December 2013 in order to understand the physico-chemical properties of Mahul Creek water. From the results of our study it was observed that the annual average COD level was 362.09 ppm which was far higher than the maximum tolerable level of 250 ppm set for inland surface water as well as for marine coastal water. The annual average conductivity was found to be 6122.81 μS cm-1 which was very much above the conductivity limit for inland surface water of 1000 μS cm-1 set by Central Pollution Control Board (CPCB) for propagation of fisheries. The annual average total alkalinity level was recorded as 166.25 ppm, which according to the UN Department of Technical Cooperation for Development (1985) was found to be strongly alkaline. The annual average hardness level of the creek water was found to be 2488.65 ppm which was above the limit of 300 ppm set by ISI. From the results of the present investigation it seems that the time has come to implement proper effluent water treatment techniques and enforcement of pollution control by the regulatory authority on the indiscriminate discharge of industrial wastewater into water bodies.
Evaluation of water in the southern part of Leginski Lake and its usefulness for recreation was carried out on the basis of the following criteria: Kohl 1975 and Kavka 1987 in Albinger's modification; U.S Department of the Interior, Federal Water Pollution Control Administration; and the European Committee for the Quality of Water for Bathing Purposes. Research work was aimed at determining the number of bacteria indicatory of pollution (Total Viable Count 20°C) and sanitary state (FC and FS). Samples of water and bottom sediments were examined at three different sites. The results revealed that water in the southern part of Leginski Lake can be used for recreational purposes. The majority of samples examined was classi­fied as clean or little polluted. A higher degree of water loading was found only at the outlet of the stream coming from biological ponds of the local water-treatment plant.
Progressive degradation of water ecosystems may contribute to the propagation of fungi pathogenic to humans and animals. The aim of this study was a quantitative evaluation and identification of species of potentially pathogenic fungi in the littoral zone of the Sulejów Reservoir used as recreation site. We studied samples of surface water and sediments collected from 6 sampling sites of the reservoir in the years 2000 and 2001. In 2000, fungi were isolated from 82.7% of samples, while in 2001 from as many as 95.4%. There were 28 species representing genera: Candida, Cryptococcus, Geotrichum, Kloeckera, Rhodotorula, Saccharomyces, and Trichosporon. The most common species were Rhodotorula glutinis and Candida guilliermondii. The density of the population of identified species depended on the season and sampling site and ranged from 80 to 328000 cells/dm³ (also uncountable growth). The waters of the Sulejów Reservoir may be a covenient place of anamorphic developmental forms, for the 15 cultured species which can also propagate in humans and other mammals.
Diatom indices and indicator system based on benthic diatom communities were used to estimate the water quality and its changes in the Vistula River on the sector between 10 and 337 km of its course. The results showed similar trend in the water quality changes along the river course as those obtained by chemical analyses. Significant correlation was found between the calculated index values and also between indices and selected water chemistry variables related to the organic pollution and trophic state. However, there was no correlation with the concentration of phosphates. Both results, the chemical as well as biological ones based on diatoms, showed differences in water quality along the investigated river sector. Natural, unpolluted water with low nutrient concentration was found only in the upper part of the river (up to 10.9 km of its course). The increased trophic state was observed in changes of all the calculated diatom index values and in the relative abundance of the indicators for eutrophic stage already from 36.6 km, raising up together with organic pollution level along the river course. The heavy pollution occurred mainly on the section from 66.2 km to 185.2 km. At the last part of the investigated sector especially on 336.7km, an improving tendency was observed. The seasonal changes in the diatom community structure and values of diatom indices were connected with fluctuations of current velocity observed in 1997 during big flood at the beginning of July. Distinct differences were found in August and October 1997, when the structure of diatom communities and the values of diatom indices indicated the lowest water quality in the whole investigated sector.
Certain features of leeches make them potentially very useful in the biological assessment of freshwaters as bio-indicators of water pollution, especially in moderately polluted lowland watercourses. The main aim of the study was to test their usefulness as indicators of the level of habitat degradation. The composition of leech samples and the main abiotic parameters in water samples, which had been taken simultaneously in six lowland streams of north-eastern Poland and the relations between them were investigated. Multivariate methods of data analysis were used to test if particular leech species or the taxonomic composition of assemblages thereof could be useful in predicting the assessed quality of the environment. The greatest difficulty with analysis of the results is to separate the effect of the stream (site specifics) from the effect of environmental quality, expressed as Cumulative Index of Environmental Quality (CIEQ). An important result of this study is the highly consistent prediction of the level of water pollution to be attained on the basis of leech taxonomic composition using Discriminant Function Analysis. Some common leech species were found to be good, positive or negative indicators of pollution level. The classifications of Glossiphonia complanata and Erpobdella octoculata as negative bio-indicators corroborates the conclusions of numerous previous studies, in contrast with the indicative value of Helobdella stagnalis, shown previously to be rather a negative indicator. It would seem to be necessary, for biomonitoring purposes, to identify detailly the leeches of such ecologically different families as Glossiphonidae and Erpobdellidae.
New sorbents capable of forming hydrogen and it-jt bonds with phenols were prepared. These sorbents possess aromatic Tt-acceptor residues and hydrogen donor and acceptor sites. The surroundings of the sites are of different polarities. Sorption efficiency of these materials was studied by reversed-phase high performance liquid chromatography. The testing mixtures consisted of three phenols of different polarity and acidity.
Zooseston of the Vistula River section almost 340 km long was investigated in the years 1997/98. In 99 samples collected 90 species of rotifers, 16 cladocerans, 9 copepods and other animals belonging to: Harpacticoidea, Oligochaeta, Nematoda, Chironomidae, Odonata, Simulidae, Tardigrada and Coelenterata were found. Multiple regression analysis showed that the number of rotifers is significantly correlated with basic chemical indicators of water trophic state - phosphate, nitrate and nitrite as well as with the number of copepods which are usually predators. The numbers of copepods depends on the availability of possible prey, i.e. rotifers and cladocerans. Multiple regression confirmed known dependence of cladocerans from trophic conditions. Clustering of similarity matrices showed complex structure of sestonic assemblages on rhitral-potamal gradient additionally modified by hydrotechnical constructions. These constructions broke old river continuum. Ordination of sites gave complex pattern not only representing a simple gradient rithral - potamal but also all transient stages caused by hydrotechnical construction (large dam reservoir) or by inflows of polluted waters from the tributaries. Ecological meaning of principal component ordination (PC) for river zooseston assemblages is not simple and might be susceptible of various interpretations.
The following paper is a part of the complex studies and intends to provide the background for explaining spatial differentiation of plant and animal communities settled in the Upper Vistula River. The study has been conducted from 10.9 to 336.7 km of the river course. Physico-chemical parameters of water, and nutrient concentrations m surface sediment have been measured. This allowed to discern four zones according to the level of water pollution. The first one, with relatively clean waters, is located in the mountainous part of the Vistula River (to 10.9 km of the river course). The second one - moderately polluted - spreads to 66.2 km. The third zone, with heavily polluted waters, reaches to 248.2 km. The remaining part of the studied river course (to 336.7 km) can be considered as belonging to the fourth zone with considerably polluted waters.
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