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1

Effects of intracortical microinjection of vitamin B12 on penicillin-induced epileptiform activity in rats

100%
Erfanparast A., Tamaddonfard E.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr 2
There are increasing studies indicating neuroprotective effects for vitamin B12. In the present study, the effect of intracortical microinjection of vitamin B12 was investigated on penicillin-induced epileptiform activity. We also examined the effects of intracortical microinjection of diazepam (a GABA-benzodiazepine receptor agonist) and flumazenil (a GABA- benzodiazepine receptor antagonist) to clarify the possible mechanism of vitamin B12. In urethane-anesthetized rats, epileptiform activity was induced by intracortical microinjection of penicillin (300 IU, 1.5 ^l), and the number and amplitude of spike waves were analyzed using electroencephalographs (EEG) recordings. Intracortical microinjections of vitamin B12 at doses of 100 and 200 ng/site, diazepam at a dose of 200 ng/site and their ineffective doses (50 ng/site of vitamin B12 with 50 ng/site of diazepam) co-microinjection treatment significantly (P<0.05) reduced both the number and amplitude of spike waves. In addition, combined microinjection of effective doses of vitamin B12 (100 ng/site) and diazepam (200 ng/site) produced more antiepileptiform effect in comparison with their alone used doses. The antiepileptic effects induced by microinjection of vitamin B12 and diazepam at a same dose of 200 ng/site were prevented by the same site microinjection of 50 ng/site of flumazenil. The results showed antiepileptiform activities for vitamin B12 and diazepam at the cerebral cortex level. A central GABA-benzodiazepine receptor complex-mediated mechanism might be involved in the antiepileptiform activity of vitamin B12.
2

Effect of lacto-ovo-vegetarian diet on serum vitamin B12 concentrations - five-year prospective study

100%
Madry E., Lisowska A., Chabasinska M., Przyslawski J., Schlegel-Zawadzka M., Walkowiak J.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2009
|
tom 08
|
nr 4
71-76
Background. Avoiding of meat is the basic principle of vegetarian diet with several variants existing. In the present study we have focused on lacto-ovo-vegetarian (LOV) diet which includes dairy products and eggs. We have aimed to assess its long-term effect on vitamin B12 status and intended to verify the influence of natural and vitamin B12- -fortified food. Material and methods. The prospective study was carried out comprising 16 healthy omnivores (12 females, 4 males) who intentionally declared to follow strict LOV diet for at least 5 years. The studied group was divided into 2 subgroups. First subgroup followed LOV diet based entirely on natural products while the second consumed food fortified with B12 vitamin. Evaluation of vitamin B12 concentration was performed before and after 6, 12, 24 and 60 months from LOV diet implementation. Results. Five-years of LOV diet resulted in significant decrease of serum vitamin B12 concentrations in the whole studied group (p < 0.05). However, the significant drop of vitamin B 12 level was in fact limited to the subgroup consuming exclusively natural products (p < 0.05). In none of the subjects abnormal serum vitamin B12 levels were observed. Conclusions. Five-year lacto-ovo-vegetarian diet did not result in a risk of vitamin B12 deficiency in healthy subjects. However, the levels of serum vitamin B12 concentrations in a subgroup consuming natural diet tend to be significantly lower as compared with the group consuming fortified food. It confirms the need of follow-up (and potentially vitamin B12 fortification of food and/or its supplementation) in long-term lacto-ovo- -vegetarians
3

Fermented multi-vegetable juices supplemented with Propionibacterium cell biomass

84%
Warminska-Radyko I., Laniewska-Trokenheim L., Gerlich J.
Polish Journal of Food and Nutrition Sciences
|
2006
|
tom 15/56
|
nr 4
Pasteurized multi-vegetable juices were fermented using the inoculum with the following composition: L. plantarum, L. brevis and B. bifidum. After the fermentation, centrifuged cells of P. acidipropionici and P. jensenii were added to juices at a dose of 109 cfu/cm3 of juice. The juices were stored at a temperature of 5oC for 21 days, with periodical controls of the cell count of introduced bacteria, and subjected to a sensory analysis. In fresh juices, the numbers of all genera of lactic and propionic fermentation bacteria were similar (ca. 109 cfu/cm3) and did not change until day 10 of storage. Deterioration of taste was proceeding gradually in consecutive days of storage and on the 21st day of storage the juices were rated 2.5-3.61, on average, in a five-point scale, Survivability of bacteria introduced into juices was good and their population numbers in the 21st day of storage were found to range from 3.9x107 to 6.8x108 cfu/cm3. The inoculated biomass of Propionibacterium cells delivered vitamin B12 whose content in all fresh juices was alike and accounted for 8.62-8.71 µg/100cm3, and was not changing over the storage period.
4

Wlodzimierz S.Ostrowski. Fifty years of research

84%
Laidler P.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 4
5

Homocysteine level in urine of autistic and healthy children

67%
Kaluzna-Czaplinska J., Michalska M., Rynkowski J.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 1
 Homocysteine is an amino acid which plays several important roles in human physiology and is an important biomarker for possible deficiencies of various vitamins (vitamin B6 and B12, folic acid). In this work GC-MS method was used to determine the levels of homocysteine in the urine of autistic and healthy children. The levels of homocysteine in urine samples from 34 autistic and 21 healthy children were 2.36 ± 1.24 and 0.76 ± 0.31 (mmol∙mol-1 creatinine), respectively. The higher level of homocysteine in autistic children may indicate deficiencies of folic acid and vitamins B6 and B12 in nutrition of these children. The results of this work were taken into consideration in the nutrition of autistic children treated in the Navicula Centre of Diagnosis and Therapy of Autism in Łódź (Poland).
6

Determinants of plasma homocysteine in coal miners

67%
Mungan A. G., Can M., Kiran S., Acikgoz S., Guven B.
Acta Biochimica Polonica
|
2013
|
tom 60
|
nr 3
Aim: Several studies suggest that coal miners are under risk of severe health problems such as cardiovascular, pulmonary, neurological, renal, hematological and musculoskeletal disorders. However, there are limited data on biochemical changes in underground workers. In our study we aimed to evaluate the association between serum homocysteine (Hcy), vitamin B12, cystatin C and folate levels in the blood of underground coal miners. Materials and Methods: Eighty one coal miners who work as underground or surface workers were recruited into our study. The study population was divided into two groups: the surface worker group (control group, n=33) and the underground worker group (n=48). The folate, vitamin B12, Hcy, cystatin C levels and body mass indexes (BMI) of both groups were measured and compared. Serum folate, Hcy and vitamin B12 levels were measured with a competitive chemiluminescence immunassay. Serum levels of cystatin C were determined by the latex particle-enhanced turbidimetric method using a cystatin C kit. Urea values were measured with a kinetic method on an automated analyzer. Results: There were no statistically significant differences between the underground workers and surface workers in the urea, cystatin C and vitamin B12 levels. High serum Hcy levels and low folate levels were found in underground workers compared with those in surface workers. The correlation between Hcy and folate levels was also statistically significant. Similarly, there was also a significant correlation between Hcy and vitamin B12, and between Hcy and cystatin C levels. Conclusions: Elevated Hcy levels may be associated with underground working but further research is necessary to understand the relation between elevated Hcy and increased prevalence of health problems in coal miners.
7

Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: preliminary results

67%
Milic M., Rozgaj R., Kasuba V., Orescanin V., Balija M., Jukic I.
Acta Biochimica Polonica
|
2010
|
tom 57
|
nr 3
The aim of this study was to find correlations between folate and vitamin B12 on baseline damage in white blood cells and their association with smoking, alcohol consumption and ageing. Thirty-six healthy vitamin non-deficient male subjects were selected in a randomized study. Comet assay (SCGE) and micronucleus (MN) assay were used as biomarkers of DNA damage. The amount of DNA damage was correlated with vitamin B12 and folic acid concentration. Positive, but non-significant correlation (canonical R = 0.61; χ2 = 28.97; P = 0.253) was found between micronucleus (MN) frequency or comet assay parameters (SCGE) and five covariates (age, smoking, alcohol consumption, vitamin B12 and folate blood serum concentration). The highest MN frequency was observed in the group with the lowest vitamin B12 concentration (F = 3.59; P = 0.024). The SCGE assay failed to show significant correlation with vitamin B12 or folic acid concentration. Concentration of vitamin B12 was significantly correlated with incidence of micronuclei. Our results present background data that could be valuable for future genotoxicological monitoring.
8

Hyperhomocysteinemia among Omani autistic children: a case-control study

67%
Ali A., Waly M. I., Al-Farsi Y. M., Essa M. M., Al-Sharbati M. M., Deth R. C.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 4
 High serum homocysteine (Hcy) level is regarded as an indicator for impairment of folate-dependent methionine cycle and is associated with oxidative stress. In a case control study, we evaluated eighty 3-5 years old Omani children (40 diagnosed with Autism Spectrum Disorder and 40 their age and gender matched controls) for their fasting serum homocysteine levels as a biomarker of Autism Spectrum Disorder (ASD). Serum folate and vitamin B12 status were also evaluated. The serum homocysteine was measured using an enzyme immunoassay (EIA) technique whereas folate and vitamin B12 were measured using an automated random access immune-assay system. The results indicated that mean serum Hcy levels were significantly (P < 0.05) higher in autistic children (20.1 ± 3.3 µmol/L) as compared to controls (9.64 ± 2.1 µmol/L). Significantly (P < 0.05) lower serum folate (1.8 ± 0.4 µg/L) and vitamin B12 (191.1 ± 0.9 pg/mL) levels were observed in autistic children as compared to controls (6.1 ± 0.6 µg/L and 288.9 ± 1.3 pg/mL, respectively). The levels of homocysteine in autistic children were also much higher as compared to normal reference values (5-15 µmol/L). The results suggest that high fasting serum homocysteine and low folate and vitamin B12 levels could be used as clinical biomarkers for an early diagnosis and management of ASD.
9

Influence of addition of cobalamin to the extender on the post-thaw motility, viability, and DNA integrity of bovine ejaculate

67%
Mizera A., Kuczaj M., Szul A., Jedraszczyk J.
Medycyna Weterynaryjna
|
2019
|
tom 75
|
nr 03
The present study was undertaken to investigate the effect of various concentrations of cobalamin (vitamin B₁₂) in Bioexcell® extender on the spermatozoa quality of Simmental bulls. The semen was collected from 12 bulls by means of an artificial vagina. Vitamin B₁₂ at concentrations of 1.50, 2.50, 3.50, and 5.00 mg/mL was added to bovine Bioexcell® extender. The semen aliquots were cooled and preserved at 4°C. Their quality was evaluated during pre-freezing, and then the cooled semen samples were packaged into 0.25 ml straws. The straws were frozen in the vapor of liquid nitrogen, and stored at -196ºC in a container. The straws were thawed one day later, and the characteristics of spermatozoa were examined. The results showed that the effect of vitamin B₁₂ on the viability, DNA fragmentation and motility of spermatozoa was significant under both pre- and post-freezing conditions (p <0.05), and revealed that supplementation of the extender with B₁₂ improved the post-thaw spermatozoa quality in bulls.
10
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Ocena potrzeb i możliwości zwiększenia zawartości witaminy B12 w diecie

67%
Sicinska E., Cholewa M.
Roczniki Państwowego Zakładu Higieny
|
2012
|
tom 63
|
nr 1
Wprowadzenie. Witamina B12 to grupa związków o aktywności biologicznej zbliżonej do cyjanokobalaminy. Biodostępność witaminy B12 z różnych produktów różni się znacznie np. z mięsa kurcząt wynosi średnio 61-66%, ryb 42%, natomiast z jaj <9% Stosunkowo łatwo uzupełniać niedobory witaminy B12 spożywając regularnie produkty wzbogacone dostępne na rynku lub suplementy diety. Cel badań. Celem badań była charakterystyka dostępnych na rynku produktów wzbogaconych witaminą B12 oraz ocena możliwości zwiększenia pobrania tej witaminy poprzez włączanie takich produktów do codziennej diety. Materiał i metoda. Badanie przeprowadzono zimą 2011 roku, w 11 sieciach handlowych w Warszawie. Dane o produktach zaczerpnięto z informacji zawartych na opakowaniach. W sprzedaży znaleziono 220 produktów z dodatkiem tej witaminy B12. Dodawana ona była przede wszystkim do płatków śniadaniowych, soków, napojów bezalkoholowych, batonów zbożowych, cukierków, kakao i herbat granulowanych, margaryn, ale również do produktów sojowych. Najliczniejszą grupę produktów stanowiły płatki śniadaniowe (40%) oraz soki i napoje bezalkoholowe (30%). Wyniki. Najwyższą zawartością witaminy B12 charakteryzowały się niektóre cukierki (max. 4,5μg/100g) i napoje herbaciane (max. 3,75μg/100g), a najniższą napoje owocowe (min. 0,12μg/100g). Szeroka oferta produktów z dodatkiem witaminy B12 umożliwia zwiększenie spożycia tej witaminy, na przykład poprzez spożycie szklanki napoju sojowego (20,8% normy na poziomie RDA), kubka deseru sojowego (15%), napoju herbacianego (14%), szklanki soku jabłkowego (12,5%), batonu zbożowego (10%), porcji płatków kukurydzianych (9,8%) czy kromki pieczywa z margaryną (7,5%). Oszacowano, że jednorazowe spożycie średniej porcji produktów z dodatkiem witaminy B12 może dostarczyć jej 0,18-0,5 μg (7,5-20,8% normy RDA dla osób dorosłych). Wnioski. Aby produkty spożywcze wzbogacone spełniały pozytywną rolę w żywieniu potrzebna jest odpowiednia edukacja żywieniowa społeczeństwa, ze szczególnym zwróceniem uwagi na osoby starsze oraz osoby stosujące diety wegetariańskie.
11

Selective cobalamin malabsorption and the cobalamin-intrinsic factor receptor

67%
Grasbeck R.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 4
The disease is characterised by cobalamin (Cbl) deficiency in children 0-5 years old, causing failure to thrive, infections, megaloblastic anaemia, neuropathy, and mild general malabsorption; slight proteinuria is common. Cbl injections produce remission, but Cbl malabsorption and proteinuria persist. About 250 cases have been reported. Dogs also have it. The heredity is autosomal and recessive. The physiological and pathological absorption mechanisms are described: Cbl liberated from food by digestion is first bound to haptocorrin, but in the intestine it is transferred to intrinsic factor. In the ileum the complex attaches to a receptor on the enterocytes; this requires neutral pH and Ca2+. The receptor is a membrane-bound glycoprotein consisting of multiple subunits. The receptor-ligand complex is endocytosed and degraded in lysosomes, and the vitamin is transferred to transcobalamin which carries it to tissues. The same receptor is strongly expressed in the kidneys, but urine also contains its activity which can be assayed for diagnosis. The basic lesion is an error in the ileal receptor. In the affected dogs the synthesised receptor is retained intracellularly. Urine and ileal biopsies from human cases contained little receptor but it had conserved affinity for the ligand. Recently examined Arab patients did not excrete reduced amounts of the receptor. Apparently, the disease has subsets, such as different structural errors in the receptor and possibly faulty transport inside the enterocyte. The cause of the proteinuria is unknown but kidney damage due to severe Cbl deficiency and an error in a multiligand renal receptor are among the possibilities.
12

Carrageenan as a support material for immobilization of propionic acid bacteria

67%
Czaczyk K., Trojanowska K.
Food Science and Technology. Scientific Papers of Agricultural University of Poznan
|
1998
|
tom 02
13

Batch propionic acid fermentation using Propionibacterium sp. immobilized in different supports

67%
Czaczyk K., Trojanowska K.
Biotechnologia
|
1998
|
nr 1
14
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Wplyw obnizenia poziomu energii w dawce pokarmowej na poziom witaminy B12 w osoczu krwi buhajkow

59%
Lozyca-Kaplon M., Oler A., Glowinska B., Rajs R.
Acta Scientiarum Polonorum. Zootechnica
|
2010
|
tom 09
|
nr 3
43-48
Badaniami objęto 60 buhajków: 30 rasy polskiej holsztyńsko-fryzyjskiej odmiany czarno-białej (PHF) oraz 30 mieszańców krów rasy polskiej holsztyńsko-fryzyjskiej z buhajami rasy limousine (PHF x LM). Oceniano wpływ obniżenia poziomu energii w dawce do 80% zapotrzebowania bytowego w ostatnich 60 dniach opasu na koncentrację witaminy B12 w osoczu krwi. Stwierdzono, że zmodyfikowana dieta nie wpłynęła istotnie statystycznie na zawartość kobalaminy we krwi buhajków PHF x LM. Wykazano natomiast istotny statystycznie spadek poziomu witaminy B12 u buhajków PHF po tygodniu od zmiany dawki, co nie potwierdziło się jednak na koniec doświadczenia.
15

Rozmieszczenie i wspolzaleznosci zawartosci witamin B6 i B12 w roznych czesciach lososi norweskich i baltyckich

59%
Lebiedzinska A., Zbikowski R.
Roczniki Państwowego Zakładu Higieny
|
2007
|
tom 58
|
nr 2
417-422
Ryby stanowią istotne źródło składników odżywczych, w tym witamin z grupy B. W niniejszej pracy oznaczano stężenie witamin B6 i cyjanokobalaminy w częściach łososi norweskich i bałtyckich pochodzących z rynku metodą wysokosprawnej chromatografii cieczowej (HPLC). W badaniach stwierdzono istotne korelacje (p<0,05; p<0,01; p<0,001) zawartości witamin B6 i B12 (cyjanokobalaminy) w badanych częściach łososi norweskich i bałtyckich.
16

Dieta, homocysteina, choroby sercowo-naczyniowe

51%
Klosiewicz-Latoszek L., Ostrowska A.
Bromatologia i Chemia Toksykologiczna
|
2000
|
tom 33
|
nr 4
381-387
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