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The aim of this work was to determine the content of Mn, Zn, Cu, Pb, Cr, Ni and Cd in auto- and semihydrogenic soils of Bialowieza National Park and to find relationships between their occurrence and litho- and pedogenic features of these soils. Basing on geometric mean contents of the elements studied a quantitative series of their occurrence was established as follows: Mn (297) >Zn (29) >Cr (16) >Pb (12) >Ni (10) >Cu (8.0) >Cd (0.29) mg kg-1 d.w The distribution of the above trace elements in soil profiles is linked to the direction of progress of the soil forming processes and, to some extent, to the variability of soil texture (especially in not completely developed soils). In general, the mineral-organic (A) horizons were least abundant in Cr, Ni, Cu, Zn and Cd, followed by the illuvial (Eet) and bedrock (C) horizons of the soils studied. Statistical analysis shown a significant correlation between contents of Cr, Ni and Zn and clay and colloidal particle amounts as well as between the content of Pb and the amount of organic carbon
In this study we determined the levels of trace elements (zinc, copper, lead, cadmium and mercury) in three layers of bones of the hip joint (cartilage, compact bone and spongy bone) of 30 red foxes (Vulpes vulpes) from north-western Poland. Concentrations of Cu, Zn, Pb and Cd were determined by atomic absorption spectrophotometry (ICP-AES) in inductively coupled argon plasma using a Perkin-Elmer Optima 2000 DV. Determination of Hg concentration was performed by atomic absorption spectroscopy. In cartilage, compact bone and spongy bone samples from the red fox, median concentrations of the metals studied could be arranged in the following descending series: Zn > Cu > Pb > Cd > Hg, the values ranging from 142 to 0.002 mg/kg dw. There was a significant difference in Cu concentrations, among all the materials analyzed, with much more Cu found in spongy bone than in compact bone. Significant differences were also noted in the case of Hg concentrations in cartilage with compact bone and the spongy bone, and between concentrations of this metal in compact bone and spongy bone. In males, the concentration of Hg in spongy bone was greater than in females. Younger foxes had a higher concentration of this metal in cartilage than adults. The strongest synergistic relationships were observed in spongy bone between the Zn and Cu, Zn and Cd, as well as between Cu and Cd. Statistically significant antagonistic relationships were detected between zinc and lead in compact bone. In addition to monitoring studies conducted on the abiotic environment, an urgent need exists for long-term monitoring of concentrations of heavy metals with long-term effects on living organisms. An important addition is provided by biomonitoring studies on domesticated and free-living mammals, including Canidae.
The content of selected trace metals (Cd, Cr, Hg, Ph, Zn) and their distribution in superficial water, bottom sediments and in muscular tissue of fishes (roach, perch, bream) and mollusks (taken from the Zemborzyce Reservoir) were examined using atomic absorption spectrometry (AAS). A comparison of the trace elements content in investigated constituents of the Zemborzyce Reservoir was carried out. Superficial water contains micro-trace quantities of examined metals. Bottom sediments are characterized by a smaller content of Cd, Pb and Zn, in comparison to the previous data. Accumulation of Cd is very similar for all examined fishes. The highest level of Cr was found in roaches. Perches indicate best accumulation ability of Hg, Pb and Zn. Mollusks show excellent accumulation ability to all studied elements.
It has been proposed that plant metallothionein (MT) sequesters excess copper, and possibly zinc, thereby preventing adverse metal-protein interactions. These metals can accumulate either gratuitously in response to other nutritional deficiencies or in plants grown in either copper- or zinc-enriched medium. Data are presented which confirm that in pea roots grown in low available iron there is increased (i) copper accumulation, (ii) MT transcript abundance, (iii) ferric-chelate reductase activity and (iv) cupric-chelate reductase activity. It is also shown that in roots grown in iron supplemented medium MT transcripts accumulate in response to elevated exogenous zinc. However, contrary to expectations, depletion of exogenous copper below normal micronutrient levels also confers an increase in the abundance of MT transcripts. The hypothesis that the products of plant metallothionein genes could act as copper chaperones is discussed.
Contamination of soils around mines by heavy metals has not yet received the serious attention that it deserves in South Africa. The current study evaluated the concentrations and levels of pollution by trace metals in soils around a Ferro-chromium mine in South Africa. Soil samples were collected from 20 locations in four different directions, namely southwest, southeast, northwest, and northeast of the mine. The soils were analyzed for trace metals concentrations using ICP-MS. The result revealed that soil pH was in the acidic medium with a very low level of soil organic matter. The concentrations of elements from the soil followed the order Al > Fe > Ca > Mg > Cr > Na > Mn > Ni > Zn > V > Cu > Pb > As > Cd. Higher concentrations for all the elements were recorded from the topsoil and also from the southwestern direction, and the differences in the concentrations were significant (p> 0.05). A highly significant positive correlation of Fe and Cr with Ni and with each other (0.42 ≤ r ≥ 0.82) were recorded. The Pi (pollution index) and I-geo (geoaccumulation index) indicated that the soils around the mine were severely contaminated with Cr and Ni. The concentrations of Fe, Cr, and Ni from the soil samples were high enough to cause serious health problems for people living in the area.
The results of a pot experiment on soil application of sewage sludge samples (at the rate of 200 g DM • pot-1) from 19 sewage treatment plants localized in region of the Green Lungs of Poland in three areas: the Great Masurian Lakes, Iława Ostróda Lake District and Łyna River Basin, are discussed in the paper. The sewage treatment plants had different capacities, received different shares of industrial sewage water and used different technologies of sewage sludge dewatering. In the pot experiment the following five vegetable crops were grown (without winter break): root celery, lettuce, small radish, Swiss chard and spinach. Before planting the first plant (celery) and after harvesting the last plant in the rotation (spinach), physical and chemical properties of the soil were studied in order to determine if the amendment of soil with sewage sludge could pose any environmental risk to the growth of vegetable crops. It was found that most of the applied samples of sewage sludge did not produce adverse effects on the determined parameters. In many cases, the content of organic carbon increased soil retention properties were improved under the effect of sewage sludge. Sewage sludge form Zalewo, because of its high content of chromium, was implied as being hazardous for growing plants, as it could result in soil and plant contamination. When sewage sludge from Olsztyn, Olsztynek and Spychowo was applied to soil, zinc contamination occurred.
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Metals phytotoxicity assessment and classification

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In this paper, the influence of trace metals (Cd, Pb, Cu, Co, Ni, Zn) on plants of spring barley (Hordeum vulgare L.) was investigated in polluted sod podzolic sandy loam on layered glacial sands and calcareous deep chernozem on loamy loess soils. We propose to estimate the phytotoxicity with help of phytotoxicological classification. The phytotoxicological classification of trace metals gives the possibility to assess their hazard for plants. On the base of indicators such as plant up-taking index (UI), phytoletal dose (PhLD₅₀), Dipole moment (µ), Phyto Maximum Allowable Concentration (PMAC) a phytotoxicological classification of hazardous trace metals was suggested. The four classes of danger in phytotoxicological classification of hazardous trace metals were offered. According to phytotoxicological classification, Cd, Co, Ni belong to the first class of hazard, Cu – to the second class of hazard, Zn – to the third class of hazard, Pb – to the fourth class of hazard. Phytotoxicological classification of hazardous trace metals gives the possibility to comprehensively estimate the danger of trace metals for plants as a biological object that plays a very important role in the life of ecosystem. This approach may be applied for another trace metals risk assessment for other plants.
Comparability of data collected within collaborative programmes became the key challenge of analytical chemistry in the 1990s, including monitoring of the marine environment. To obtain relevant and reliable data, the analytical process has to proceed under a well-established Quality Assurance (QA) system with external analytical proficiency tests as an inherent component. A programme called Quality Assurance in Marine Monitoring in Europe (QUASIMEME) was established in 1993 and evolved over the years as the major provider of QA proficiency tests for nutrients, trace metals and chlorinated organic compounds in marine environment studies. The article presents an evaluation of results obtained in QUASIMEME Laboratory Performance Studies by the monitoring laboratory of the Institute of Meteorology and Water Management (Gdynia, Poland) in exercises on nutrient determination in seawater. The measurement uncertainty estimated from routine internal quality control measurements and from results of analytical performance exercises is also presented in the paper.
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