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At present, major causes of diseases is oxidative stress affecting both metabolic and physiological functions of the body. That is why there is a great need for investigation of nutritious food supplements for counteracting these oxidative stresses. Therefore, the aim of study was to evaluate the therapeutic potential of Apium leptophyllum Pers. fruits by estimating total phenolic as well as flavonoidal contents and antioxidant values. The collected fruits were extracted separately using different solvents like methanol, ethanol and water. Total phenolic and flavonoid contents were measured from the respective extracts and correlated with their antioxidant values. The antioxidant properties of various fruit extracts (12.5, 25, 50, 100 and 150 μg/ml) were evaluated by DPPH, hydroxyl, nitric oxide and superoxide scavenging assay and compared with ascorbic acid as a standard. All the extracts of A. leptophyllum were found to be dose dependent inhibition against these free radicals. Among all these extracts, the methanolic one was found better in the scavenging activity and followed dose-dependent manner against DPPH, hydroxyl radical, nitric oxide, superoxide anions with minimum IC50 values of 97.9, 89.02, 135.37, 127.73 μg/ml, respectively, and also observed more significant (p<0.01) as compared with standard. Furthermore, total phenolic and flavonoidal contents were found highest in methanolic extract. The results obtained in this study clearly indicate that the methanolic extract of A. leptophyllum may be used as a new potential source of natural nutritional supplement in food or pharmaceutical industries due to rich source of phenolic, flavonoidal contents as well as antioxidant property.
The antioxidant capability and phenolic contents of ethanol extracts (free phenolics) and ethyl acetate extracts (bound phenolics) of three Quercus species were estimated in this work. The antioxidant activities were examined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical, reducing power and β-carotene bleaching methods. HPLC was employed to detect major phenolic acids. The leaf extract of Q. salicina contained maximum total phenolics while the highest total flavonoid content was found in the leaf extract of Q. serrata. The antioxidant activities varied among three species. Bark extract of Q. salicina was the most potential and it was closed to levels of the standard antioxidative dibutyl hydroxytoluene (BHT). The bark extract of Q. serrata also showed promising antioxidant activities despite their eminence was negligibly lower than Q. salicina. Stronger antioxidant activities of free phenolics than those of the bound phenolics may be attributed to higher quantities of free phenolics in the barks of Quercus species, however total flavonoids may not contribute a critical role. By HPLC analysis, thirteen phenolic acids were detected in the leaf and bark extracts. Of them, Q. salicina showed maximum in number (ten compounds) and quantities of detected phenolic acids. Ellagic, chlorogenic and benzoic acids were dominant in Quercus species. Findings of this study revealed that leaves and barks of three Quercus species are rich source of antioxidants, and Q. salicina is the most promising and should be elaborated to exploit its pharmaceutical properties.
Alpinia zerumbet (Pers.) B.L. Burtt. & R.M. Sm is an important perennial ginger plant in the tropics with various uses as foods, dietary supplement, cosmetics, essential oil production and traditional medicines. In this study, essential oils, phenolic acids, fatty acids, kavains, total phenols and antioxidant activity in A. zerumbet plants exposed to UV-C were investigated. Number and weights of essential oils increased rapidly from 1 to 3 days of exposure. Major extend of essential oils was recorded in methyl cinnamate and followed by benzyl acetone. The correspondence to the accumulation of palmitic acid was 15 folds, in contrast to the strong reduction of stearic quantity was 10 folds at 3 days of treatment. Changes in chemical components and antioxidant capacity in A. zerumbet are differed reversely between the exposure of UV-C and heavy toxic chemicals. The exposure of UV-C is beneficial for the increasing of essential oil production in A. zerumbet.
This study focused on examining the effect of vegetables extract foliar application on storage quality of the grapes grape (Vitis vinifera L. cvs. ‘Perlette’ and ‘King’s Ruby’). The vines were sprayed after fruit set twice at 15 days interval at concentrations of 0, 0.20%, 0.30% and 0.40%. Fruit were harvested and kept under cold-storage at 0.5 ±0.5°C and 90% RH for 28 days. The physicochemical properties of treated and untreated berries were analyzed at 7 days intervals during the cold storage. The results showed that the pre-harvest application of vegetable extracts reduced the weight loss and inhibited the fruit decay. Treated grape also had higher ascorbic acid contents, total phenolics, 2,2-diphenyl-1-picrylhydrazyl-radical (DPPH) scavenging activity, and higher soluble solid contents, titratable acidity, and reduced sugar:acid ratio. The concentration of 0.30% and cultivar ‘Kings Ruby’ gave the best result as compared to untreated control. In conclusion, pre-harvest vegetables extract application on berries showed higher antioxidant activities in grape berries, and maintained their post-harvest quality. These results indicated that foliar spray of vegetables extract could effectively improve the fruit quality, mainly through the reduction of phenolic content.
Heracleum lasiopetalum Boiss, Kelussia odoratissima Mozaff., and Echinophora platyloba DC. belong the Apiaceae family. They are Iranian endemic plants. These three herbs have been used as food additives in traditional preparations such as pickles. Antioxidant activity (AA) of methanol extracts (ME) of the plants was evaluated by three assays, including DPPH, FRAP, and TEAC. From all three assays, comparing all the MEs for their IC50 and EC1 values, E. platyloba had the highest AA. Total phenolic content (TPC) of the extracts ranged from 74 to 120 mg TAE/g. The extract of H. lasiopetalum exhibited the highest TPC. The flavonoids content (FC) of the extracts ranged from 7.63 to 14.52 mg RE/g, from which the extract of E. platyloba had the highest flavonoids concentration. A positive correlation between the FC and AA in DPPH assay was found. A significant correlation was also found between the TPC and AA in FRAP assay. These results suggested that the level of AA in these plants varied in a great extent. Our results indicated that extract of E. platyloba could be an important dietary source of flavonoids compounds with high antioxidant capacity. In addition, E. platyloba can be used as an alternative preservative and natural flavor instead of synthetic ones in food industry (especially pickles).
Cherries are known as health friendly fruits due to their abundant phytochemical compositions. This study was conducted to determine phytochemical and pomological fruit properties of different cherry species including mahaleb (Prunus mahaleb L.), wild sweet cherry (Prunus avium L.), wild sour cherry (Prunus cerasus L.), two sweet cherries (‘Napoleon’ and ‘Starks Gold’) and one sour cherry (‘Kütahya’) cultivars. For this aim, together with various pomological traits, total phenolics and anthocyanin contents, antioxidant capacity, organic acids, sugars, were analyzed in fruits of relevant genotypes. Results of all examined traits significantly varied between genotypes. Mahaleb showed the highest TSS (30.17%), fructose (8.71 μg/g) and glucose (20.74 μg/g) contents. Wild sour cherry gave the highest antioxidant capacity (13.25 mmol TE/ kg total weight), anthocyanin (351.0 mg Pg-3-glk/kg total weight), citric acid (0.56 μg/g) and malic acid (2.96 μg/g) contents. As a rootstock, mahaleb was found to be superior in some of the traits when compared to wild sweet cherry. Significant correlations were observed between various traits. Additionally, principal component analysis (PCA) revealed different relationships among the traits and evaluated genotypes.
Chilling is one of the major abiotic stress which limites yield and quality of many crops. The seedlings of rice varieties namely Koshihikari (Japonica subtype) chilling tolerant, and the susceptible NMR2 (Indica subtype) were treated at 25/15 °C and 5/4 °C day/night to determine the growth parameters, phenolic contents, and antioxidant activity. It was found that in all treatments, the growth of MNR2 including root and shoot lengths, and leaf and root weights were inhibited at greater levels than Koshihikari. There were seven phenolic acids identified in leaves of Koshihikari including caffeic acid, vanillin, ferulic acid, sinapic acid, benzoic acid, ellagic acid, and cinamic acid, but only benzoic acid and ellagic acid were found in leaves of MNR2. In contrast, only vanillic acid and ellagic acid were observed in roots of Koshihikari, whilst ellagic acid and cinnamic acid were found in roots of MNR2. It was found that rice reduced amount of phenolic acids but promoted quantity of total phenolic content (TPC) and total flavonoid content (TFC) and level of antioxidant activity in chilling stress, although the level of responses varied between Japonica and Indica subtypes. Tolerant rice possessed greater bound flavonoids, phenolics and phenolic acids, but susceptible rice accumulated greater free TPC and TFC in reduced temperature. Findings of this study highlighted that phenolic constituents in bound forms of phenols, polyphenols, and flavonoids may play an active role in rice plants than phenolic acids under chilling stress but need further elaboration.
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