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Flower development of Ranunculus asiaticus L. growing in the University Bo­tanic Garden was divided into six stages (I - VI): tight bud stage (I), loose bud stage (II), half open stage (III), open flower stage (IV), partially senescent stage (V) and senescent stage (VI). The average life span of an individual flower after it is fully open is about 5 days. Membrane permeability of sepal tissues estimated as electrical conductivity of ion leachates (^S), increased as the development proceeded through various stages. The content of sugars in the petal tissues increased during the flower opening period and then declined during senescence. The soluble proteins registered a consistent decrease with the simultaneous increase in specific protease activity and a-amino acid content during different stages of flower development and senescence. The content of total phenols registered an initial increase as the flowers opened, and then declined during senescence.
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The influence of light reduction on the growth and phenolic compounds contents in Scots pine trees and seedlings was studied. Results of the first experiment in field conditions show that shade causes an increase of phenolic compounds and in the second experiment with seedlings under controlled shading conditions the results were opposite. It is suggested that the factor insolation should be paid more attention during the studies on the influence of pollution on the content of phenolic compounds.
Throughout the world, charcoal rot, caused by Macrophomina phaseolina, is one of the most destructive and widespread diseases of crop plants such as soybean. In this study, the biological control capability of 11 Trichoderma spp. isolates against M. phaseolina was investigated using screening tests. Among all the tested Trichoderma spp. isolates, inhibition varied from 20.22 to 58.67% in dual culture tests. Dual culture, volatile and non-volatile tests revealed that two isolates of Trichoderma harzianum (including the isolates T7 and T14) best inhibited the growth of M. phaseolina in vitro. Therefore, these isolates were selected for biocontrol of M. phaseolina in vivo. The results of greenhouse experiments revealed that disease severity in the seed treatment with T. harzianum isolates was significantly lower than that of the soil treatment. In most of the cases, though, soil treatment with T. harzianum resulted in higher plant growth parameters, such as root and shoot weight. The effects of T. harzianum isolates on the activity of peroxidase enzyme and phenolic contents of the soybean root in the presence and absence of M. phaseolina were determined in greenhouse conditions. Our results suggested that a part of the inhibitory effect of T. harzianum isolates on soybean charcoal rot might be related to the indirect influence on M. phaseolina. Plant defense responses were activated as an elicitor in addition to the direct effect on the pathogen growth.
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