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The authors used Albendazole in a single dose of 400 mg in 40 students from Africa and Asia, infected with Ascaris lumbricoides, Trichocephalrus trichiurus and Ancylostomatidae. At the third day after the treatment no parasite eggs have been found in 25 of 30 cases of ancylostomatidosis (83.3%), 16 of 23 cases of trichocephalosis (69.6%) and in all treated cases of ascaridosis (100%). It has been also found that the tolerance for the medicine is good in most patients. Only in two patients (2%) there appeared short time and not very strong pains in the upper part of the abdomen and headaches. An important property of Albendazole is its ability to destroy nematode eggs which is essential for the prophylaxis and nature conservation.
Hookworms are very important blood sucking nematode parasites of humans and domestic animals. The host with a heavy infection can lose almost a cup of blood per day. This may contribute to anemia which is associated with many physical and mental developmental insults. The works on obtaining an effective hookworm vaccine have been lasting for about eighty years. Recent identifications of a number of bioactive molecules produced by larval and adult stages of Ancylostomatidae are very helpful for selecting of nematode proteins crucial for host-parasite interactions and promising vaccine antigens. Many of these molecules are involved in host skin penetration by infective larvae, intestinal tissue invasion and digestion of haemoglobin and/or other macromolecular substrates. However, the results of many vaccination trials using recombinant forms of these proteins showed no sufficient protection against experimental hookworm infections.
Apart from roundworms, Ancylostomatidae nematodes are presently the most frequently observed nematodes in dogs in Poland, with two species described so far: Uncinaria stenocephala and Ancylostoma caninum. The study aimed to determine the hookworm species found in dogs in Poland, with special emphasis on the Lublin region. The study material consisted of fecal samples collected from 500 dogs from the area of the Lublin voivodeship. With the use of microscope methods, parasite eggs were found in 56.4% of the samples, dominated by the Ancyclostomidae nematode (26.8%). The isolated hookworm eggs were subject to morphometric measurements, giving a mean length of 77.60 µm and width of 44.25 µm (±SD ±6.01 and ±4.54 respectively) within the range 67-91 × 36-56 µm. The hookworm larvae that hatched from the samples were subject to molecular analysis (72 samples) and all were identified as U. stenocephala. In the area of the Lublin voivodeship U. stenocephala is the dominant hookworm species in dogs.
Two species of hookworms from genus Uncinaria have been found so far in Poland. Uncinaria stenocephala infects mainly dog, wolf and red fox, whereas Uncinaria criniformis is a parasite of mustelids (but it was also reported from red fox). 19 male and 29 female hookworms from red foxes have been compared with 10 male and 12 female worms from dogs. Hookworms from dogs were generally smaller than these from foxes, but no other morphological differences could be found. These hookworms were qualified to species Uncinaria stenocephala on the ground of morphology of male. Genomic DNA samples have been isolated from these hookworms and segments of rDNA including part of small subunit of ribosomal RNA gene; internal transcribed spacer 1 (ITS1); 5.8 S ribosomal RNA; internal transcribed spacer 2 (ITS2) and part of large subunit of ribosomal RNA have been amplified and sequenced. Sequences from Uncinaria obtained both from foxes and dogs have shown very high similarity to the sequence of Uncinaria stenocephala, so all examined hookworms have been classified as belonging to this species.
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