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1

A novel, potent and selective inhibitor of the activity of inducible nitric oxide synthase [GW274150] reduces the organ injury in hemorrhagic shock

100%

McDonald M. C., Izumi M., Cuzzocrea S., Thiemermann C.

Journal of Physiology and Pharmacology

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2002

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tom 53

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nr 4,1

An enhanced formation of nitric oxide (NO) by the inducible NO synfhase (iNOS) may contribute to the pathophysiology of hemorrhagic shock. This study investigates the effect of a novel, potent and selective inhibitor of iNOS activity (GW274150) on the circulatory failure and the organ injury and dysfunction associated with hemorrhagic shock in the anesthetised rat. Hemorrhage (sufficient to lower mean arterial blood pressure to 45 mmHg for 90 min) and subsequent resuscitation with shed blood resulted (within 4 h after resuscitation) in a delayed fall in blood pressure, renal and liver injury and dysfunction as well as the pancreatic injury. Pre-treatment of rats with GW274150 (5 mg/kg at 30 min prior to the onset of hemorrhage) attenuated the renal dysfunction as well as the liver and pancreatic injury caused by hemorrhage and resuscitation. Interestingly, GW274150 did not reduce the delayed fall in blood pressure associated with hemorrhagic shock. We propose that an enhanced formation of NO from iNOS contributes to the organ injury and dysfunction in hemorrhagic shock, and that highly selective inhibitors of iNOS activity, such as GW274150, may represent a novel therapeutic approach for the therapy of hemorrhagic shock.

2

The search for a genetic defect in Polish patients with chronic granulomatous disease

100%

Jurkowska M., Kurenko-Deptuch M., Bal J., Roos D.

Archivum Immunologiae et Therapiae Experimentalis

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2004

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tom 52

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nr 6

3

Endothelial protection from reperfusion injury by ischemic preconditioning and diazoxide involves a SOD-like anti-O2 mechanism

100%

Maczewski M., Duda M., Pawlak W., Beresewicz A.

Journal of Physiology and Pharmacology

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2004

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tom 55

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nr 3

Cardiac ischemia/reperfusion leads to coronary endothelial dysfunction, mediated by superoxide anion (O2-), but not hydroxyl radical (.OH). Ischemic preconditioning and mitochondrial ATP-dependent potassium channel opener (diazoxide) protect endothelium in the mechanism involving attenuation of O2- burst at reperfusion. We hypothesize that the endothelial protection involves upregulation of myocardial anty-O2- defense. Langendorff-perfused guinea-pig hearts were subjected to global ischemia/reperfusion (IR) or were preconditioned prior to IR with three cycles of ischemia/reperfusion (IPC) or infusion/washout of 0.5 µM diazoxide. Coronary flow responses to acetylcholine were measures of endothelium-dependent vascular function. Myocardial outflow of O2- and of .OH during reperfusion and myocardial activities of superoxide dismutase (SOD) and catalase were measured. IR impaired acetylcholine response and augmented cardiac O2- and .OH outflow. IPC, diazoxide, and SOD (150 IU/ml) attenuated O2- outflow, increased .OH outflow and protected endothelium. There were no differences in Cu/Zn-SOD, Mn-SOD and catalase activities between sham-perfused and IR hearts and only catalase activity was increased in the IPC hearts. We speculate that: (i) IPC and diazoxide endothelial protection involves activation of some SOD-like anti-O2- mechanism resulting in attenuation of O2- burst and increase in .OH burst, (ii) improved SOD activity might have not been detected because it was confined to a small, although functionally important, enzyme fraction, like that bound to the endothelial glycocalyx.

4

The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of erythrocytes

100%

Wrobel A., Lukaszynska B., Kedzierska J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

5

Nitric oxide - superoxide cooperation in the regulation of renal Naplus, Kplus -ATPase

100%

Beltowski J., Marciniak A., Jamroz-Wisniewska A., Borkowska E.

Acta Biochimica Polonica

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2004

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tom 51

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nr 4

The aim of this study was to investigate whether endogenous superoxide anion is involved in the regulation of renal Na+ ,K+ -ATPase and ouabain-sensitive H+,K+-ATPase activities. The study was performed in male Wistar rats. Compounds modulating superoxide anion concentration were infused under general anaesthesia into the abdominal aorta proximally to the renal arteries. The activity of ATPases was assayed in isolated microsomal fraction. We found that infusion of a superoxide anion-generating mixture, xanthine oxidase (1 mU/min per kg) + hypoxanthine (0.2 umol/min per kg), increased the medullary Na+ ,K+ -ATPase activity by 49.5% but had no effect on cortical Na+ ,K+ -ATPase and either cortical or medullary ouabain-sensitive H+ ,K+ -ATPase. This effect was reproduced by elevating endogenous superoxide anion with a superoxide dismutase inhibitor, diethylthiocarbamate. In contrast, a superoxide dismutase mimetic, TEMPOL, decreased the medullary Na+ ,K+ -ATPase activity. The inhibitory effect of TEMPOL was abolished by inhibitors of nitric oxide synthase (l-NAME), soluble guanylate cyclase (ODQ) and protein kinase G (KT5823). The stimulatory effect of diethylthiocarbamate was not observed in animals pretreated with a synthetic cGMP analogue, 8-bromo-cGMP. An inhibitor of NAD(P)H oxidase, apocynin (1 ^mol/min per kg), decreased the Na+ ,K+ -ATPase activity in the renal medulla and its effect was prevented by L-NAME, ODQ or KT5823. In contrast, a xanthine oxidase inhibitor, oxypurinol, administered at the same dose was without effect. These data suggest that NAD(P)H oxidase-derived superoxide anion increases Na+,K+-ATPase activity in the renal medulla by reducing the availability of NO. Excessive intrarenal generation of superoxide anion may upregulate medullary Na+,K+-ATPase leading to sodium retention and blood pressure elevation.

6

Decrease in 2,2,6,6-tetramethyl-piperidine-1-oxyl [TEMPO] EPR signal in peroxynitrite-treated erythrocyte membranes

100%

Wrobel A.

Cellular and Molecular Biology Letters

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2001

|

tom 06

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nr 2

7

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The influence of lead and selenium interactions on shoot growth and antioxidant enzyme activity in Vicia faba L. minor plants

84%

Mroczek-Zdyrska M., Strubinska J., Hanaka A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

8

Occurrence and source of hydrogen peroxide in aphids

84%

Urbanska A.

Electronic Journal of Polish Agricultural Universities. Series Biology

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2009

|

tom 12

|

nr 4

9

Effects of Pantoea agglomerans on the respiratory system. Part II. Studies in vivo

84%

Milanowski J.

Annals of Agricultural and Environmental Medicine

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1994

|

tom 01

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nr 1

10

Superoxide dismutase and lipid peroxidation in cerebrospinal fluid of patients with ischemic brain stroke

84%

Sonecki P., Olczyk K., Gralewski Z., Ogrodowska-Hamankiewicz E.

Acta Biochimica Polonica

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1994

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tom 41

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nr 2

11

Water deficit-induced membrane injury and oxidative stress in two barley genotypes

84%

Bandurska H., Floryszak-Wieczorek J.

Acta Societatis Botanicorum Poloniae

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2002

|

tom 71

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nr 1

12

Alcohol-related cirrhosis with pancreatitis. The role of oxidative stress in the progression of the disease

84%

Szuster-Ciesielska A., Daniluk J., Kandefer-Szerszen M.

Archivum Immunologiae et Therapiae Experimentalis

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2001

|

tom 49

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nr 2

13

Studies on the superoxide anion production and peroxidase activity in potato leaf cell suspension exposed to salicylic acid

84%

Faravardeh L., Rabbani A.

Acta Physiologiae Plantarum

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2006

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tom 28

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nr 3

It is now widely accepted that salicylic acid (SA) signaling is mediated by reactive oxygen species (ROS) production. We have studied the effect of SA on peroxidase activity and superoxide anion production in potato leaf cell suspension. The results show that potato cells are insensitive to low concentrations of exogenous SA (< 1 mM) and the effect is observed at 1-5 mM SA. The cells exposed to SA exhibit higher peroxidase activity and show different peroxidase pattern when antiyzed on native gels compared to the control. Superoxide anion production is enhanced after two hours of treatment and 2.5 mM SA gives the highest value. The results suggest peroxidase-mediated detoxification of ROS elicited by SA.

14

The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of bovine erythrocytes

84%

Wrobel A., Lukaszynska B., Kedzierska J.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 2

Bovine erythrocytes treated with peroxynitrite (ONOO-), a cytotoxic species formed in vivo via the reaction of nitric oxide (NO*) and the superoxide anion (O2-*), show an increased rate of hemolysis on sudden osmotic stress. The increase in the rate was peroxynitrite concentration dependent. In the presence of some antioxidants (uric acid, ascorbic acid, glutathione, melatonin and albumin), this effect was significantly lower, with ascorbic acid as the most efficient antioxidant.

15

Rebamipide attenuates nonsteroidal anti-inflammatory drugs (NSAID) induced lipid peroxidation by the manganese superoxide dismutase (MnSOD) overexpression in gastrointestinal epithelial cell

67%

Nagano Y., Matsui H., Shimokawa O., Hirayama A., Tamura M., Nakamura Y., Kaneko T., Rai K., Indo H. P., Majima H. J., Hyodo I.

Journal of Physiology and Pharmacology

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2012

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tom 63

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nr 2

16

Comparison of Arabidopsis thaliana (col-0) plants and cell suspension cultures responses to Alternaria brassicicola infection

67%

Rousseau K., Macioszek V. K., Kononowicz A. K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

17

Teophylline inhibits free oxygen radicals production by human monocytes via phosphodiesterase inhibition

67%

Chorostowska-Wynimko J., Kus J., Skopinska-Rozewska E.

Journal of Physiology and Pharmacology. Supplement

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2007

|

tom 58

|

nr 5

18

Oxidative stress generation in Taxus baccata leaves affected by Pestalotiopsis funerea under sunny and shaded conditions

67%

Rybus-Zajac M.

Dendrobiology

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2005

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tom 54

51-56

In the last few years on old yew trees growing in the parks and gardens extensive disease symptoms were observed on leaves, indicating affection with pathogenic microorganisms. The causal factor was the presence of a fungal pathogen, Pestalotiopsis funerea. Physiological responses involving the oxidative stress, i. e. superoxide anion and hydrogen peroxide levels, and the superoxide dismutase were analyzed as an element of a plant defense mechanism. Diseased leaves from plants growing under sunny and shaded conditions, from May to July, were investigated. The increased generation of superoxide anion-radicals and hydrogen peroxide were observed in the leaves with disease symptoms simultaneously with the activation of superoxide dismutase, which may indicate the induction of host defense response to the P. funerea.

19

Reactive oxygen species [ROS] and reactive nitrogen species [RNS] alter metabolic effect of insulin in rat L6 satellite cells

67%

Orzechowski A., Grzelkowska K.

Polish Journal of Veterinary Sciences

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2000

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tom 03

|

nr 1

This study investigates the effect of superoxide anion radical (02); hydrogen peroxide (H202), nitric oxide (NO) and peroxynitrite (ONOO), which often accompany inflamed, endotoxic or exercised muscle on insulin action in DTsatellite cells. In order to induce quiescence, rat L6 myoblasts were subjected to transition from G2/M to Gl phase by the application of serum-reduced medium. Insulin stimulating effect on cell mitogenicity and anabolism was dose-dependent and hormetic. Application of H202 alone enhanced protein synthesis with dose-dependency but had no effect on mitogenicity. While insulin and H202 were used together, (i.e. at low H202 dose) insulin action was not affected regardless of the combination used, except the loss of dose- dependency on protein synthesis, but for 100 μM of H202 insulin action ceased abruptly and totally. Since there were no additive effects of both factors, we conclude that H202 may contribute to the insulin-induced anabolic reaction, however, below 100 The application of 02- donor stimulated protein synthesis and slightly inhibited [cell proliferation] though dose-response pattern was not observed suggesting apparent limitations to 02- diffusion into the cell. Moreover 02- inhibited both insulin-enhanced mitogenicity and protein synthesis by abrogating dose-response fashion of insulin action. The introduction of NO and ONOO- donors alone to control systems inhibited cell proliferation in a dose-dependent manner having no effect on protein synthesis (except the low doses of SIN-1). Insulin-stimulated syntheses of both DNA and protein were inhibited in a dose- dependent manner by SIN-1 (NO and 02' donor). In the presence of SNP (NO donor) mitogenic effect of insulin was abolished whereas protein synthesis was diminished only by the highest SNP concentration used (0.5 mM). Taken together, these results have shown that hydrogen peroxide (H202), nitric oxide (NO) and peroxynitrite (ONOO ) provide a good explanation for developing resistance to growth promoting activity of insulin in satellite cells under conditions of oxidant stress.

20

Decreased protein nitration in macrophages that overexpress indoleamine 2,3-dioxygenase

67%

Keskin D. B., Marshall B., Munn D., Mellor A. L., Gearhart D. A.

Cellular and Molecular Biology Letters

|

2007

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tom 12

|

nr 1

The activity of indoleamine 2, 3-dioxygenase (IDO; E.C. 1.13.11.42) catalyzes the oxidative cleavage of tryptophan to form kynurenine. IDO activity consumes superoxide anions; therefore, we postulated that over-expression of IDO might mitigate superoxide-anion dependent, oxidative modification of cellular proteins in vitro. We prepared and characterized RAW 264.7 macrophages that were stably transfected with either an IDO expression vector or the control (empty) vector. We detected IDO mRNA, protein, and enzyme activity in the IDO-transfected macrophages, but not in the macrophages transfected with the empty vector. To generate superoxide anions in situ, we treated the IDO-and control-transfected cultures with xanthine or hypoxanthine, and then used ELISA methods to quantitate the relative levels of oxidatively modified proteins in total cell lysates. The levels of protein carbonyls were similar in IDO-transfected and vector-transfected macrophages; however, protein nitration was significantly less in IDO-transfected cells compared to control transfectants. In addition, steady-state levels of superoxide anions were significantly lower in the IDO-transfected cultures compared with control transfectants. Our results are consistent with the concept that, besides degrading tryptophan, IDO activity may protect cells from oxidative damage.

Ograniczanie wyników

A novel, potent and selective inhibitor of the activity of inducible nitric oxide synthase [GW274150] reduces the organ injury in hemorrhagic shock

The search for a genetic defect in Polish patients with chronic granulomatous disease

Endothelial protection from reperfusion injury by ischemic preconditioning and diazoxide involves a SOD-like anti-O2 mechanism

The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of erythrocytes

Nitric oxide - superoxide cooperation in the regulation of renal Naplus, Kplus -ATPase

Decrease in 2,2,6,6-tetramethyl-piperidine-1-oxyl [TEMPO] EPR signal in peroxynitrite-treated erythrocyte membranes

The influence of lead and selenium interactions on shoot growth and antioxidant enzyme activity in Vicia faba L. minor plants

Occurrence and source of hydrogen peroxide in aphids

Effects of Pantoea agglomerans on the respiratory system. Part II. Studies in vivo

Superoxide dismutase and lipid peroxidation in cerebrospinal fluid of patients with ischemic brain stroke

Water deficit-induced membrane injury and oxidative stress in two barley genotypes

Alcohol-related cirrhosis with pancreatitis. The role of oxidative stress in the progression of the disease

Studies on the superoxide anion production and peroxidase activity in potato leaf cell suspension exposed to salicylic acid

The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of bovine erythrocytes

Rebamipide attenuates nonsteroidal anti-inflammatory drugs (NSAID) induced lipid peroxidation by the manganese superoxide dismutase (MnSOD) overexpression in gastrointestinal epithelial cell

Comparison of Arabidopsis thaliana (col-0) plants and cell suspension cultures responses to Alternaria brassicicola infection

Teophylline inhibits free oxygen radicals production by human monocytes via phosphodiesterase inhibition

Oxidative stress generation in Taxus baccata leaves affected by Pestalotiopsis funerea under sunny and shaded conditions

Reactive oxygen species [ROS] and reactive nitrogen species [RNS] alter metabolic effect of insulin in rat L6 satellite cells

Decreased protein nitration in macrophages that overexpress indoleamine 2,3-dioxygenase