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Epidemiological studies on caseous lymphadenitis were carried out in Poland in 1996 and 2002 among goat herds covered by a milk recording program. Between-herd seroprevalence was 13.2% in 1996 and increased to 62.5% in 2002. The average size of seropositive herds was statistically significantly higher than that of seronegative ones, however there was no statistically significant difference in the age between the herds. A statistically significant prevalence ratio (PR) was identified and relevant attributable risk for exposed animals (ARexp) was calculated for the following risk factors: presence of seropositive males in a herd (PR=8.350; ARexp=0.651), presence of superficial abscesses in animals (PR=6.142; ARexp=0.620), presence of respiratory signs (PR=2.900; ARexp=0.393), presence of animals in poor condition in a herd (PR=2.774; ARexp=0.390) and occurrence of reproductive failures in a herd (PR=1.798; ARexp=0.230). Purchase of animals from abroad, mastitis and husbandry conditions (housing system, grazing system, hygienic conditions) were not shown to be statistically significant risk factors.
Invasion of Impatiens glandulifera was reconstructed along rivers in the Czech Republic, central Europe, based on floristic records. The occurrence was assessed along river banks, outside the river banks, and along tributaries downstream from the first records of the species in 1902, 1903, 1978 and 1995, respectively. The following main results were obtained: (a) The species nowadays occupies 77% of the river length in the country. (b) The rivers, investigated in detail, did not differ in the occurrence of the species along the river banks. (c) There were some significant relationships between the penetration of I. glandulifera far from the river banks both in terms of the lateral dimension in the main valley, and upstream along the tributaries, and the time of the first occurrence of the species on the river. (d) It took approximately 20 years from the first occurrence on the main river and the start of spread along the tributaries. The process of invasion is still in progress after about one century and further spread is expected.
The work involved assessment of the Myzus persicae (Sulz.) capability to infect successively potato plants with PVY and PVM after a Sunspray 850 EC mineral oil application. The tests were carried out in the greenhouse, with 4-week-old, healthy potato plants possessing low ressistance to viruses, derived from in vitro (test plants). Any time, for each combination and each virus, 10 successive plants were inoculated in 6 repetitions. Virus sources were potato plants infected with PVY or PVM, kept in isolated rooms. As a result of oil application, feeding of the M. persicae specimens on plants previously treated with this oil was delayed. The highest reduction as regards PVY and PVM transmission by M. persicae was obtained in the treatment where both plants constituting virus sources and test plants were protected, because only two of ten plants were infected with PVY, and only one with PVM. Mineral oil application only on potato test plants (healthy ones) reduced to a small degree M. persicae capability to transmit PVY to six successive plants (to seven in control), whereas it was much higher for PVM - to three (to six in control). In the case when only plants constituting virus sources were oil-protected, aphid's capability to transmit PVY was limited only to four plants, and PVM - to two. These results seem to confirm much more the hypothesis that mineral oil inactivates virus particles in the stylets of aphids while they attempt to acquire it from plants which have been previously protected with mineral oil.
In this study, we examine the pattern of occurrence of Impatiens parviflora in Central European oak forests over time and its ecological requirements within these types of communities. Research was based on phytosociological data collected in 3776 relevés. A modified TWINSPAN algorithm were used to distinguish the groups of oak forests. The ecological preferences of the I. parviflora and studied communities as well as differences between invaded and non-invaded vegetation plots were analyzed using mean weighted Ellenberg indicator values (EIVs). Finally, both the temporal pattern of I. parviflora participation and changes in its coverage in the studied communities were analyzed. Our study confirmed a high adaptability of this species with respect to temperature, moisture, soil reaction and nutrients and determined its broad ecological optimum in oak forests. However, it also revealed both a greater sensitivity of some communities within Central European oak forests to the invasion of I. parviflora and differences in habitat conditions between invaded and non-invaded vegetation plots. This suggests that the habitat niche of I. parviflora within oak forests is not accidental. The analysis of temporal changes in the frequency of I. parviflora confirmed a 20% increase in relevés over the last 50 years. However, we did not identify any statistically significant rise in the coverage of I. parviflora in oak forests specifically during the studied period.
So far in Poland, the Asian clam Corbicula fluminea has been reported from the Oder (Odra) and Vistula River. Its new population has been discovered in the Warta-Gopło Canal in Konin (central Poland), where water temperature can reach 34°C, as the canal is a part of a power plant lake cooling system. The spatial distribution of C. fluminea was found to be very uneven and that is probably why this species was not found during earlier research. The highest density of C. fluminea was 78 ind.·m⁻² at the site where water flow was the fastest (2.49 m·s⁻¹), providing sufficient oxygenation of the water. The results suggest that existing information about the distribution of C. fluminea may be far from complete because the clam can be present also in the habitats that were not regarded as suitable for the species according to earlier reports, and therefore have not been monitored for its presence.
Plasmids classified to the IncP-1 incompatibility group belong to the most stably maintained mobile elements among low copy number plasmids known to date. The re­markable persistence is achieved by various tightly controlled stability mechanisms like active partitioning, efficient conjugative transfer system, killing of plasmid-free segregants and multimer resolution. The unique feature of IncP-1 plasmids is the cen­tral control operon coding for global regulators which control the expression of genes involved in vegetative replication, stable maintenance and conjugative transfer. The multivalent regulatory network provides means for coordinated expression of all plasmid functions. The current state of knowledge about two fully sequenced plasmids RK2 and R751, representatives of the IncP-1a and IncP-1β subgroups, is presented.
The preferences for spreads and both frying and baking fats were investigated among 545 female students of Universities at Gdynia, Olsztyn, Cracow and Poznań. Preferences were expressed as the percentage of subjects indicating a particular food. The significance of 14 choice factors was determined using a 3-point scale. Butter, margarine and low-fat margarine were the most preferred spreads. Among frying and baking fats, oil was mostly used for the preparation of dishes. Most often indicated choice factors included product-related ones such as freshness, taste and durability, and for frying and baking fats, also the universality of their use. No important differences in either the division of subjects or the significance of choice factors between female students of different universities were revealed with the Friedman's ANO VA statistical test. The correlation of the rankings of foods and choice factors between pairs of universities varied from weak to very high. The greatest differences appeared for preferences of spreads and the lowest for choice factors.
The value of cathcpsin B activity determination for evaluation of the extent of disease was investigated in 98 patients with the cervix uteri carcinoma and 25 women with cervix uteri dysplasia. The measurements were performed before treatment. Cathepsin B activity was estimated in serum using Z-Phe-Arg-NMec, and in tumor tissue using Z-Arg-Arg-pNA • HC1 as substrates. The mean activity of the enzyme increased both in serum and tumor tissue with progression of neoplastic disease and was dependent on the clinical stage of cervical carcinoma. It should be stressed, however, that among the patients with the clinically observed early stage of the disease, higher cathepsin B activity was observed in those in whom metastases to pelvic lymph nodes were detected than in those in whom the disease was limited to cervix uteri.
This study investigates the effect of climate variability on the phenotype, leaf litter decomposition intensity and seedling spread of alien red oak (Quercus rubra L.). Twenty-eight red oak forest stands located in Lithuania were evaluated. Indirect climate change indicators such as continentality were used in the analysis. Simulation of climate warming was achieved using an agro sheet cover. According to the results, the morphological traits of red oak stems in the maritime regions (warmer winters) do not differ significantly from those of red oaks stems in the continental areas of Lithuania (colder winters, more frequent spring frosts). Red oak leaf litter under an agro sheet cover (warmer conditions) decomposes at almost the same intensity as without the cover (natural conditions). The red oak seedlings spread 100 m or more irrespective of continentality. These results showed indirectly that climate change will not affect red oak stem quality or microorganism activity in the process of leaf litter decomposition. Red oak will thus remain as an invasive species, threatening composition change in native forests.
Rhizoctonia solani was isolated from 1/4–2 year-old seedlings of Silver fir, European larch, Scots pine and only sporadically from Norway spruce with symptoms of stem and root rot and yellowing and browning of needles. The species occurred on at least half of analysed, diseased plants. In laboratory trials the species colonised all parts of seedlings of Silver fir, European larch, Scots pine, Norway spruce and also Common alder and birch. On inoculated leaves of alder and birch necrosis spread about twice quicker than on needles of coniferous plants. Isolates from Norway spruce and Scots pine colonised plant parts quicker than from Silver fir and European larch. In greenhouse trial all isolates caused necrosis of 4 coniferous species and alder seedlings.
The distribution of Arion lusitanicus Mabille and the damage caused by the slug were studied In 1993–2009. A total of 38 sites were examined, characterised in terms of location, time of appearance and density of the slug, type and size of crops affected, and degree of damage to plants. At the start of the 1990s A. lusitanicus occurred only around Rzeszów and in the Sub-Carpathian region. In 1997 it started to appear in other regions of Poland. The rate of its spread was found to have increased significantly, particularly in the past few years. The slug mainly colonises urban areas (30 sites), and much less often – other areas (8 sites). The slug densities were the greatest in cultivations. The degree of damage done by the slug varied among plant species. Brassicas, lettuce, beetroot, carrots, beans, strawberries, numerous ornamental flower plants and some weeds were the most damaged. The increase in the spread of A. lusitanicus poses a serious threat to the biodiversity of native habitats and to garden-cultivated plants.
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