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The evaluation of topography Borrelia burgdorferi by atomic force microscope (AFM)

100%

Tokarska-Rodak M., Koziol-Montewka M.

Health Problems of Civilization

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2015

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tom 09

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nr 3

The aim of the present work was to evaluate the topography of spirochetes’ cells Borrelia burgdorferi s.s. B31 in atomic force microscope (AFM). Results: The length of spirochetes B. burgdorferi has ranged between 15.38-22.68μm. The cells of spirochetes do not constitute structures of a fixed diameter and height. Thus, in order to identify real parameters of cells, the horizontal distance and vertical distance have been used in the measurements. The average value of a spirochetes’ diameter has been estimated by taking series of measures and it is 0.40 μm. The average value of a spirochetes’ height has been estimated by taking series of measures and it is 70.14 nm. The analysis of a relation between measured parameters of spirochetes: diameter and height revealed that along with the growth of diameter of a bacteria cell, its height also grows. The average value of a fibers’ diameter has been estimated by taking series of measurements and it is 0.09 μm and the average height of fibers was 7.91 nm. Conclusions: The atomic force microscope (AFM) is a modern tool with a broad spectrum of observatory and measure abilities and is a technique which has been used in biology and microbiology to investigate the topography of surface and in the evaluation properties of cells.

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Why is there still no human vaccine against Lyme borreliosis?

86%

Skotarczak B.

Folia Biologica

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2015

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tom 63

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nr 3

3

Expression profiles of toll-like receptors in the differentiation of an infection with Borrelia burgdorferi sensu lato spirochetes

86%

Dudek S., Ziolko E., Kimsa-Dudek M., Solarz K., Mazurek U., Wierzgon A., Kokot T., Muc-Wierzgon M.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 2

4

Occurrence of the spirochetes Borrelia burgdorferi sensu lato in ticks Ixodes ricinus [L.] from selected municipal woods of the city of Poznan

86%

Nowosad A., Jenek J., Glazaczow A., Szkaradkiewicz A.

Wiadomości Parazytologiczne

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1999

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tom 45

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nr 4

5

The fruitful coexistence of Borrelia burgdorferi and Ixodes ticks

72%

Lewandowski D., Urbanowicz A., Figlerowicz M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 1

6

Current methods used to identify and genotype spirochaetes Borreliella burgdorferi

72%

Teodorowicz P., Weiner M.

Health Problems of Civilization

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2020

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tom 14

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nr 2

Lyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland and in the world for several years. The proportion of borreliosis infections caused by B. burgdorferi spirochaetes is increasing. Difficulties diagnosing t his disease w ith conventional methods have led to growing molecular biology research aimed at developing improved diagnostic tools. Molecular biology methods include all techniques that allow the detection and analysis of nucleic acids. Among them there are methods based on PCR reaction, molecular typing methods (MLST), new generation sequencing (NGS). The current development of this field gives great research opportunities. With regard to B. burgdorferi spirochaete, molecular biology is used to: confirm Lyme borreliosis, identify and type Borreliella spirochaetes, detect them in tick vectors or intermediate hosts, as well as to identify co-infections between different Borreliella species and other tick-borne pathogens. They are meant to significantly improve diagnostic analyzes. This paper reviews the current methods used for the detection and identification of B. burgdorferi. Molecular mechanisms for the survival of spirochaetes in the host, infection vectors and clinical picture of Lyme disease were also discussed.

7

Interactions between Borrelia burgdorferi and mouse fibroblasts

72%

Chmielewski T., Tylewska-Wierzbanowska S.

Polish Journal of Microbiology

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2010

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tom 59

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nr 3

Borrelia burgdorferi spirochetes are an infectious agent of Lyme borreliosis. The aim of our studies was to investigate the fate of engulfed B. burgdorferi cells in L-929 mouse fibroblasts and to observe development of intracellular infection in vitro after 2 and 48 h. Electron microscopic studies reveal consecutive stages of B. burgdorferi spirochetes penetration to mouse fibroblasts in vitro. It has been observed, as a first step attachment and engulfment of spirochetes followed by formation of vacuoles. After 48 hours of infection, vacuoles of fibroblastic cells have been seen full of B. burgdorferi bacteria and latter they have been released from infected cells to extracellular space. It can be the evidence that B. burgdorferi multiply intracellulary.

8

The cytotoxic properties of Serpulina hyodysenteriae

72%

Binek M., Szynkiewicz Z., Spohr de Faundez I., Wojcik U., Klimuszko D., Rzewuska M.

Acta Microbiologica Polonica

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1995

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tom 44

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nr 1

9

Characterization of Brachyspira sp. strains isolated from flock of hens with diarrhoea

72%

Kizerwetter-Swida M., Rzewuska M., Binek M.

Bulletin of the Veterinary Institute in Puławy

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2005

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tom 49

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nr 2

10

Alpha fucosidase and beta galactosidase in serum of a Lyme disease patients as a possible marker of accelerated senescence — a preliminary study

58%

Wasiluk A., Waszkiewicz N., Szajda S. D., Wojewodzka-Zelazniakowicz M., Kepka A., Minarowska A., Zwierz Z. W., Pancewicz S., Zalewska-Szajda B., Ladny J. R., Zwierz K.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 2

11

Phylogenetic diversity of flaB gene among Borrelia spp. in Ixodes ricinus ticks from urban and semi-natural habitats

58%

Kowalec M., Szewczyk T., Welc-Faleciak R.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

12

What is percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae?

58%

Nejedla P., Norek A., Vostal K., Zakovska A.

Annals of Agricultural and Environmental Medicine

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2009

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tom 16

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nr 2

273-276

13

Adaptation factors of Borrelia for host and vectors

58%

Skotarczak B.

Annals of Agricultural and Environmental Medicine

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2009

|

tom 16

|

nr 1

1-8

14

Ectobiotic and endocytobiotic bacteria associated with termite flagellate Joenia annectens

58%

Radek R., Hausmann K., Breunig A.

Acta Protozoologica

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1992

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tom 31

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nr 2

15

Passerine birds as a natural reservoir of Borrelia burgdorferi sensu lato spirochetes in the Masurian Lake District

58%

Gryczynska A., Zgodka A.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

16

Isolation of Borrelia afzelii from overwintering Culex pipiens biotype Molestus mosquitoes

58%

Zakovska A., Capkova L., Sery O., Halouzka J., Dendis M.

Annals of Agricultural and Environmental Medicine

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2006

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tom 13

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nr 2

A total of 662 samples (winter period: 469; summer period: 193 specimens) of female mosquitoes of the genus Culex, Aedes and Anopheles were collected during the period March 2000-April 2001 from the locality of Vysoké Mýto (Eastern Bohemia, Czech Republic). They were examined by dark field microscopy for the presence of spirochetes. The motile spirochetes were observed in 4.2% of all species of investigated mosquitoes. One spirochetal strain out of the 8 isolation attempts (BRZ14) was obtained (cultivation rate was 12.5%) and the spirochetal strain was then successfully cultivated and identified using PCR for the presence of Borrelia burgdorferi s.l., and subsequently with the RFLP as genomospecies Borrelia afzelii. This strain was derived from overwintering Culex (Culex) pipiens biotype molestus female mosquitoe. This is apparently one of the sporadic cases of the occurrence of pathogenic borreliae in haematophagous arthropods, other than Ixodes ricinus complex ticks.

Ograniczanie wyników

The evaluation of topography Borrelia burgdorferi by atomic force microscope (AFM)

Why is there still no human vaccine against Lyme borreliosis?

Expression profiles of toll-like receptors in the differentiation of an infection with Borrelia burgdorferi sensu lato spirochetes

Occurrence of the spirochetes Borrelia burgdorferi sensu lato in ticks Ixodes ricinus [L.] from selected municipal woods of the city of Poznan

The fruitful coexistence of Borrelia burgdorferi and Ixodes ticks

Current methods used to identify and genotype spirochaetes Borreliella burgdorferi

Interactions between Borrelia burgdorferi and mouse fibroblasts

The cytotoxic properties of Serpulina hyodysenteriae

Characterization of Brachyspira sp. strains isolated from flock of hens with diarrhoea

Alpha fucosidase and beta galactosidase in serum of a Lyme disease patients as a possible marker of accelerated senescence — a preliminary study

Phylogenetic diversity of flaB gene among Borrelia spp. in Ixodes ricinus ticks from urban and semi-natural habitats

What is percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae?

Adaptation factors of Borrelia for host and vectors

Ectobiotic and endocytobiotic bacteria associated with termite flagellate Joenia annectens

Passerine birds as a natural reservoir of Borrelia burgdorferi sensu lato spirochetes in the Masurian Lake District

Isolation of Borrelia afzelii from overwintering Culex pipiens biotype Molestus mosquitoes