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Small angle X-ray scattering (SAXS) is one of the methods used for the analysis of the starch structure. In this manner, involving changes in electron density structure of starch becomes available. By variation of the media in which starch is suspended wider differentiation of starch structure is possible. Usually such studies are carried out in water. In this paper the SAXS method was successfully used for investigation of starch structure in such media as water, methanol, 1 M solution of HCl, 1 M solution of NaCl, and 0.1 M solution of I2 in KI. Analysis of SAXS curves for those suspensions proved some differences in curve pattern. Especially interesting data were obtained from a comparison of SAXS curves for starch suspensions in 1 M HCl and 1 M NaCl. Effective scattering of starch suspension in 1 M HCl in the initial part of the curve, as compared to the 1 M NaCl case, indicates that the diffusion of СГ ions to various regions of the starch structures depends, among other things, on the pH of the solution. These results demonstrated that the SAXS method could be used for identification of the starch structures to which specific media diffuse and, therefore, for investigating the impact of various factors on this diffusion.
The small angle X-ray scattering (SAXS) pattern of the homotetrameric aspa­raginase II from Escherichia coli was measured in solution in conditions resembling those in which its crystal form was obtained and compared with that calculated from the crystallographic model. The radius of gyration measured by SAXS is about 5% larger and the maximum dimension in the distance distribution function about 12% larger than the corresponding value calculated from the crystal structure. A compari­son of the experimental and calculated distance distribution functions suggests that the overall quaternary structure in the crystal and in solution are similar but that the homotetramer is less compact in solution than in the crystal.
Small Angle X-ray Scattering (SAXS) has been applied to the study of starch structure in wheat flour. The quality of the range changes of starch microstructure after milling was analysed and a certain relationship of SAXS scattering magnitudes with alveographic study was shown.
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