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Cough sensitivity is increased in patients with atopic dermatitis, although they have no clinical symptoms from the lower airways. In the present study we examined the cough sensitivity to capsaicin in patients, who had no clinical respiratory symptoms, with sclerodermia localized to the skin. Cough sensitivity was defined as the lowest capsaicin concentration, which evokes 2 or more coughs. Twelve patients and 12 healthy matched volunteers, as a comparison group, inhaled deep breaths (2 L) of a capsaicin aerosol in doubled concentrations (from 0.02 to 200 µmol/L). Cough sensitivity, expressed as a geometric mean (95% CI) of capsaicin concentration, was 0.15 µmol/L (0.04 to 0.56) in the patients with localized sclerodermia and 4.96 µmol/L (2.50 to 9.85) in controls, which made a significant difference towards higher cough sensitivity in sclerodemia, respiratory symptom-free patients. Thus, disease processes localized outside the respiratory tract may have surreptitious pulmonary manifestation that is brought to light by the capsaicin cough test.
There are many theories of aging and a number of them encompass the role of mitochondria in this process. Mitochondrial DNA mutations and deletions have been shown to accumulate in many tissues in mammals during aging. However, there is little evidence that these mutations could affect the functioning of aging tissues.
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Non-lipophilic mycobiota of human skin

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The human skin is inhabited by many species of bacteria and fungi, which are its natural microbiota. Fungi colonizing the skin, including those causing disease, characterized by great variety and variability, can be influenced by various factors. The purpose of this study was to investigate the composition of the non-lipiddependent fungal microbiota of skin, including the presence of species potentially pathogenic for humans. Fifty-six volunteers of both sexes aged 22–78 were subjected to the study. Swabs were taken from the face, chest, back and interdigital spaces of hands. Mycobiota isolated proved to vary both in terms of the location of occurrence and gender of patients. Interdigital spaces of hands, dominated by yeasts, constitute a location on human skin most contaminated with fungi. Molds were more often isolated from the face and chest. The back was the least contaminated location. There was no difference in fungal incidence in relation to sex.
Introduction. In the human skin exposed to ultraviolet (UV) radiation, melanogenesis occurs in two stages, accompanied by urinary excretion of Thormalen-positive melanogen (TPM). In Poland, no data are available on the course and intensity of melanogonesis in relation to UV exposure in an industrial region. Material and methods. The Thormalen test was used for the collected samples (N=136) as modified by Matous and Suchoń. Results. Maximum environmental TPM content (0.67 μg/dm3) was observed in August. Conclusion. The time of UV exposure and local type of solar radiation promote melanogenesis.
The objective of this study was to evaluate the gross effect of ointments made from Senecio vernalis extract on the healing of cutaneous wounds of rats. Thirty adult female albino rats (average weight of 250±10 g) were randomly divided into six groups. Experimental wounds were made on the dorsal thoracic region of anesthetised rats. Four groups were treated with specific ointment concentrations consisted of 3.75%, 7.5%, 15%, and 30% weight/weight Senecio vernalis ethanol extract in Eucerin™ ointment base; one group was treated by the ointment basis alone (basis) and no treatment was applied to the remaining group (control). Wound healing was assessed by computerised planimetry. Data were analysed statistically using a repeated measures mixed model. Statistical evaluation of mean wound area differentiation between groups, showed no significant changes in overall rate of the wound healing (P>0.05). However, comparisons within each group, revealed that in three groups (3.75%, 7.5%, and 15% extract concentration) a significant wound size reduction happened earlier. These results deny strong hypotheses about the desirable wound healing effects of S. vernalis contrary to other similar plants of the genus Senecio, and anti-ulcer effects of Senecio brasiliensis (a similar species to S. vernalis) in the duodenum and stomach of rats and mice.
We investigated the effect of native and modified high density lipoprotein (HDL) and low density lipoprotein (LDL) on the viability and the membrane fluidity of normal and diabetic fibroblasts. Under experimental conditions, HDL did not affect the cultured normal cells. Incubation of HDL with diabetic fibroblasts resulted in a negligible decrease in the cell viability and induced an increase in the lipid fluidity at surface of the cell membranes. LDL at the higher concentration reduced the viability both normal and diabetic fibroblasts and also enhanced the fluidity of membrane lipids. The above changes in the fluidity of fibroblast membranes were observed mainly in the presence of native and oxidized lipoproteins and are connected with the differences in the structure of cell membranes and nature of the cytotoxicity of LDL and HDL.
Lipophilic corynebacteria isolated as natural flora of human skin were examined. Among 119 assayed strains 94% presented a hydrophobic cell surface and 75.6 % were able to form biofilms. These attributes, as well as aggregation in liquid media, were statistically connected with each other and promote the developing of biofilms on solid surfaces. This was characteristic of all the lipophilic Corynebacterium species found on human skin that were examined in this study. C. jeikeium and CDC group G2 strains dominated in this population, and they could be responsible for investigated features in the whole lipophilic skin bacterial population. These two groups are the most common coryneform bacteria isolated from nosocomial infections and these attributes most likely promote them to cause opportunistic infections.
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