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The study was conducted on four beef cannulae put into forestomach of 500 kg mass in the Latin square system. The control group, apart from corn silage, was fed with hay, while experimental groups were fed trefoil silage, darnel or meadow trefoil-darnel mixture. The parameters characterizing changes taking place in the rumen as well as the number of bacteria and protozoa were determined in the rumen fluid collected before feeding and 1.5 h and 3 hours after feeding. The substitution of hay with silage caused the increase of N-NH3 concentration in the rumen and changed the fermentation profiles expressed by the increase of the amounts of the following acids: propanoic, pentanoic and iso-pentanoic, and the decrease of the following acids: acetic and butyric. The silage of meadow trefoil caused the increase of bacteria and protozoa count, while the darnel silage and meadow trefoil-darnel mixture only caused the increase of bacteria count, and reduced the protozoa count.
The aim of these studies was to assess the effect of chemical conservants (FA = formic acid, PA = propionic acid, FPA+i = formic acid, propionic acid and ammonium ions) on the cell counts of yeasts and mould fungi in silage. The silage was prepared from corn (Zea mays L), cultivar Buran FAO (240). The effect of the applied conservants on silage aerobic stability was also assessed. The performed chemical analyses comprised the determination of: the content of dry matter (DM), lactic acid (LA), acetic acid (AA), ethanol, water soluble sugars (WSC), crude protein (CP) and pH. The applied preparations were found to reduce the number of yeast and mould fungi cells in all the examined silages. The growth of fungi was inhibited most strongly by the FPA+i preparation (containing a mixture of formic and propionic acids and ammonium ions). The yeast cell counts dropped (P«0.05) from 8.50 107 CFU g-1 silage in the control (CCS = control corn silage) to 2.60 107 CFU g' in silage treated with FPA+i, whereas counts of the mould fungi cells - from 15.20 104 CFU g-1 silage in the control to 4.60 104 CFU g-1 in silage treated with FPA+i. The applied conservants increased (P«0.05) the content of DM from 255.00 g • kg 1 in control to 266.60 g • kg-1 with PA, WSC from 27.10 g • kg 'DM to 30.50 g • kg -1DM with FPA+i and ethanol from 9.10 g • kg 'DM to 11.21 g • kg -1DM with FPA+i in the silage. The additives decreased concentrations of lactic acid, acetic acid and crude protein concentration after opening the barrels. The diversifying factors decreased the pH value in the examined silage. The experimental conservants were found to improve the aerobic stability of silages after 7 days of air exposure.
Effect of ensiling pumpkin Cucurbita maxima with the addition of inoculant or without it on chemical composition and quality of silages. The aim of the studies was to determine the effect of ensiling the pumpkin on chemical composition and quality of silages. The silages were produced from pumpkin of Justynka variety. Before ensiling, the disintegrated pumpkin fruits were mixed with the dried beet pulp in ratio equals 80 : 20. Two variants of silages were prepared: with the inoculant and without it. In the silages, the following basic chemical composition was determined: the content of dry matter, crude ash, crude protein, crude fat, crude fibre, NDF, ADF and ADL. The indicators, being an evidence of the run of ensiling process and the quality of the obtained silages, were also determined, i.e. pH, lactic, acetic and butyric acids, ammonia nitrogen, ethanol and aerobic stability. In the obtained silages, as compared to the material before ensiling, the lower content of crude fibre and ADF was found whereas in the silage with inoculant, NDF level was also lowered. The silages with the inoculant were characterized by higher content of lactic and acetic acids and lower level of nitrogen, ammonia and ethanol. The silages with the inoculant had also higher aerobic stability. The conducted studies indicate that the application of inoculant has affected the improvement of the quality of the obtained silages.
Forage from genetically modified (GM) maize in two consecutive years (1999 and 2000) was ensiled in bins of 120 l volume in two combinations: with formic acid (85%) and without. In the samples of ensilage material GM maize, basic parameters have been determined such as pH and dry matter. The determination of biological activity of the components of translational apparatus in model translation systems showed the complete inactivation of biological activities. In addition, degradation of nucleic acids in examined silages was discovered.
The objective of this study was to determine the influence of biological silage additive (Bonsilage) on the hygiene quality and nutritive value of maize and grass-legume silages. The experiments were conducted on FAO 240 maize (Zea mays L.) and a mixture of italian ryegrass (Lolium multiflorum L.), 50% with alfalfa (Medicago media Pers.), 50%. Group 1 was a control and comprised silage without any additives, group 2 was ensiled with the addition of 4 cm3 kg-1 biological silage additive. After 60 days of silage process individual silages were subjected to microbiological composition, and chemical analyses of silages were also determined. Similar analyses were repeated at day 7 following exposure to oxygen. The applied biological silage additive was found to reduce (P<0.05) numbers of Clostridium, Enterobacteriaceae, yeasts and mold fungi cells, and increase (P<0.05) the number of LAB (lactic acid bacteria) in comparison with the control in both silages. Chemical analysis of the maize silage showed that the biological additive caused an increase (P<0.05) in DM (dry matter), CP (crude protein), WSC (water soluble carbohydrates), LA (lactic acid), AA (acetic acid), ethanol, and a decrease (P<0.05) in the concentration of BA (butyric acid), N-NH3 and pH value in comparison with the control. Chemical analysis of silage samples from the grass-legume mixture showed that the additive caused an increase (P<0.05) in the content of DM, CP, WSC, LA and AA in comparison with the control. Samples of silage with the addition of an inoculant were characterized by a lower (P<0.05) content of BA, N-NH3, ethanol and pH value. The biological additive impoved the aerobic stability of silages in the aerobic phase.
The study was conducted in the years 2005-2008 in the fields of the Experimental Station Swadzim (52o26’ N; 16o45’ E) belonging to ZDD Gorzyń, property of the Poznań University of Life Sciences. Two experiments with PR 39G12 maize cultivar were conducted in fields, where previously maize was sown in a six-years monoculture. The aim of the study was to evaluate the effects of natural fertilizers, straw or intercrop applied together with mineral fertilizers. We investigated whether this approach can reduce the negative effects of maize cultivation in monoculture system and at the same time help to increase the silage yield. The assessment of plant response was made on two soil classes. It has been shown that the use of natural fertilizers, straw or an intercrop (rye with vetch), reduced the negative effects of maize growing in monoculture and led to a significant increase in the dry mass yield of whole plants on IIIa soil class.
Changes in the amino acid profile of protein during ensiling depend on the proteolytic potential of plant species, the rate of the wilting and acidifying of ensiled herbage and, on the other hand, on the microbiological processes and fermentation of amino acids. The interaction of these factors determines the subsequent efficiency of nitrogen utilization in the rumen. The aim of this study has been to determine the influence of ensiling in round bales on changes in the amino acid content in lucerne and red clover silages. The amino acid composition was determined by analyzing the content of amino acids in herbages and silages. The lucerne silage protein was characterized by a higher contribution of all amino acids except Trp in comparison with red clover silage. Ensiling in bales of wilted lucerne and clover herbage lowered the content of Asp, His (P < 0.01), Ile, Lys (P < 0.05) in both plants. The ensiling process and plant species affected (P < 0.05) the total content of amino acids per 100 g of protein, where the range of changes in both raw materials was different. The loss of amino acids observed during the ensiling of wilted lucerne in round bales should be regarded as typical, whereas changes in the amino acid profile of red clover should be considered as atypical. A significantly higher ADIN content in red clover than in lucerne silage indicates that a rapid and significant increase of temperature occurred in experimental bales, which were characterized by a low degree of density, and this temperature rise strongly affected the availability of amino acids of the red clover protein also to the current microflora in the silage. Ensiling in round bales changed the amino acid profile of the lucerne and red clover protein. The ensiling process in this technology, however, severely deteriorated the quality of the red clover protein by lowering the content of all essential amino acids except Cys and Met.
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