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Background. Avoiding of meat is the basic principle of vegetarian diet with several variants existing. In the present study we have focused on lacto-ovo-vegetarian (LOV) diet which includes dairy products and eggs. We have aimed to assess its long-term effect on vitamin B12 status and intended to verify the influence of natural and vitamin B12- -fortified food. Material and methods. The prospective study was carried out comprising 16 healthy omnivores (12 females, 4 males) who intentionally declared to follow strict LOV diet for at least 5 years. The studied group was divided into 2 subgroups. First subgroup followed LOV diet based entirely on natural products while the second consumed food fortified with B12 vitamin. Evaluation of vitamin B12 concentration was performed before and after 6, 12, 24 and 60 months from LOV diet implementation. Results. Five-years of LOV diet resulted in significant decrease of serum vitamin B12 concentrations in the whole studied group (p < 0.05). However, the significant drop of vitamin B 12 level was in fact limited to the subgroup consuming exclusively natural products (p < 0.05). In none of the subjects abnormal serum vitamin B12 levels were observed. Conclusions. Five-year lacto-ovo-vegetarian diet did not result in a risk of vitamin B12 deficiency in healthy subjects. However, the levels of serum vitamin B12 concentrations in a subgroup consuming natural diet tend to be significantly lower as compared with the group consuming fortified food. It confirms the need of follow-up (and potentially vitamin B12 fortification of food and/or its supplementation) in long-term lacto-ovo- -vegetarians
The composition of fatty acids of serum lipids, the levels of serum lipids (total cholesterol, LDL-cholesterol, HDL-cholesterol, VLDL-cholesterol, triglycerides), mineral components (sodium, potassium, calcium, magnesium) and antioxidant vitamins (ascorbic acid, retinol, carotene, tocopherol) were determined in men aged about 25 years. The serum of hypertensive subjects (systolic blood pressure > 140 mmHg or more) contained, in relation to normotensive subjects, significantly less linolic acid, docosahexaenoic acid and oleic acid, but the total level of unsaturated fatty acids was not different between these two groups. No differences were found in the serum levels of the determined mineral components between normotensive and hypertensive men.
The study aimed to assess the within-herd Neospora caninum exposure in dairy cattle in southern Romania, based on the detection of specific antibodies in milk and serum. A total of 104 paired samples of milk and serum were collected from four dairy farms. Individual samples were analyzed for N. caninum antibodies by ELISA: IDEXX Neospora Ab (Idx) (three farms: A, B, C; n = 60) and ID-VET Lab (Idv) (farm D; n = 44). Additionally, four pooled milk samples, one per each farm (A, B, C) and a composed one (A+B+C), were analyzed with Idx ELISA. Optimized cut-off values for milk samples were determined by receiver operating characteristic (ROC) analysis, with serum results considered as true status. The agreement was expressed by K values. The overall seroprevalence of N. caninum infection was 45% in the farms tested by Idx ELISA and 56.8% in the farm tested by Idv ELISA. A good agreement between serum and milk was obtained for both ELISA kits (K = 0.72 and 0.77, respectively). The specificity and sensitivity at optimized cut-off of S/P>0.704 for Idx and S/P%>7.966% for Idv were 100% and 70.37% for Idx and 89.47% and 88% for Idv. Testing pooled milk samples, there were identified as N. caninum positive the dairy farms with a 15% or higher within-herd seroprevalence at the cut-off value of S/P>0.51. This is the first study in Romania in which milk samples were tested to determine the N. caninum infection status in dairy farms, providing a base for further researches.
Rozwojowi przedwczesnych chorób układu krążenia, takich jak zawał mięśnia sercowego, często towarzyszą zaburzenia w metabolizmie lipidów, szczególnie hipertrójglicerydemia i dyslipoproteinemia. Praca ta zaznajamia ze stosowaną nomenklaturą i omawia kolejne etapy w metabolizmie lipidów i lipoprotein w zdrowiu i chorobie.
The aim of the study was to investigate acid-base changes and to determine some serum biochemical parameters in dehydrated calves with diarrhoea according to the degree of dehydratation. Thirty diarrhoeic dehydrated calves submitted to treatment in the university clinic, were used in the study. The calves were accessed as suitable for this study, if they were moderately or severely dehydrated according to clinical symptoms. All sick calves had the usual yellow and watery diarrhoea. Calves with 4% to 8% dehydration (moderate) had a weak suckle reflex, dry mucous membranes, warm mouth and partly good muscular tone. Calves with 10% and above dehydration (severe) were unable to stand, and had no suckling reflex and cold mouth. The mean pH, base excess, chloride (only severely dehydrated group) and sodium were -significantly decreased in both moderately and severely dehydrated groups. On the other hand, potassium, phosphorus, HCO₃⁻ and anion gap levels were increased, compared to that of the control group. Results of this study showed that there was a relationship between the base excess and anion gap, with a degree of clinical dehydration. However, there may not be an exact correlation between the degree of dehydration and the severity of acidosis. Based on the clinical symptoms, the results of this study could be a useful tool under field conditions, in estimating the base excess in diarrhoeic dehydrated calves, when acidosis therapy is needed.
The study was investigated the effects of 10-day treatment with chelidonine (50 and 100 mg/kg ip) on the concentration of creatinine and urea in the blood serum of rats subacutely intoxicated by copper. Subacute intoxication with copper chloride has been found to cause a significant decrease in the urea level and no effect 011 the creatinine level. Chelidonine had virtually 110 effect on the parameters examined except for the decrease in urea in the dose of 50 mg/kg as compared with the control group. 10-day treatment with both doses of chelidonine significantly decreased the level of creatinine and increased the level of urea (dose 100mg/kg) in the serum of intoxicated rats.
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