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Silverthiosulphate which is a potent inhibitor of ethylene action was found to be ineffective in delaying senescence of detached flowers of Iris germanica whereas cycloheximide, a protein synthesis inhibitor, effectively delayed the senescence of these flowers and extended the longevity to 6 days. However, this treatment resulted in suppression of bud opening. When cycloheximide treatment was given at progressive intervals it became less effective in inhibiting bud opening and delaying senescence. Cycloheximide treatment maintained a higher protein content in the perianth tissue of flowers compared to untreated flowers.
 We analysed the level of polyamines (PAs) bound to thylakoids and the level and activity of thylakoid transglutaminases throughout barley leaf senescence, retarded by kinetin. The level of PAs bound to thylakoids changed in senescing barley leaves: bound putrescine (PU) and spermidine (SD) increased throughout senescence, whereas bound spermine (SM) decreased. Kinetin diminished the increase in thylakoid-bound PU and SD and almost completely abolished the decrease of the bound SM. These data suggest different roles of PU/SD and SM in thylakoid degradation. Immunodetection of transglutaminases (TGase) in thylakoid fraction revealed three bands of 33, 58 and 78 kDa. During senescence the intensity of all bands increased and it was correlated with an increase in TGase activity. Kinetin down-regulated the accumulation of the 58- and 78-kDa TGases and the TGase activity. We postulate that formation of covalent bonds between PAs and proteins by TGase is involved in chloroplast senescence. The kinetin-mediated preservation of low TGase levels and activity throughout leaf senescence may represent an important component of the mechanism of kinetin action in the retardation of leaf senescence.
Analysis of relationships between traits is an important stage in research. These results determine the decisions taken at further stages of research as well as those made when realising successive experiments. The paper presents results of an analysis of relationships between twelve traits of two types of maize (Zea mays L.) cultivars differing in leaf blade senescence rates: ES Palazzo and ES Paroli SG. Analysed traits: assimilation surface area (ASA), ear weight (EW), ear weight fraction (EWF), leaf area index (LAI), leaf area ratio (LAR), leaf weight (LW), leaf weight fraction (LWF), number of leaves (NL), plant weight (PW), specific leaf area (SLA), stem weight (SW) and stem weight fraction (SWF). Recorded results indicate positive correlations between: LW–SW, LW–PW, EW–SW, PW–SW, EW–PW, EW–EWF, SLA–LAR and negative correlations of LW–SLA, LAR–SW, PW–SLA, PW–LAR, SWF–EWF for both types of maize cultivars in all the three years of experiments.
Dementia is a neurological condition manifested by reduced cognitive and psychological functioning, altered behaviour and decreased autonomy for social and professional performance in activities of daily living. Dementia is one of the most important causes of disability in the elderly. The number of dementia patients is increasing, as the population is growing older. Recent epidemiological evidence suggested a 2001 prevalence of 24.3 million cases of dementia worldwide. Alzheimer’s disease (AD) remains the most common cause of dementia, responsible for 60–70% of cases in Europe. There are 4.6 million new cases of dementia reported every year – that’s one new case every 7 seconds. Patients with AD gradually lose the ability to function independently and interest in life and many experience changes in personality, social withdrawal, uninhibited behaviour, and psychotic symptoms such as delusions, hallucinations and aggressive behaviour. The associated likely impact on the health and quality of life of persons with AD, their families, and societal healthcare and welfare resources, have led many authors to describe the condition as a “ticking bomb”. The depression prevalence in later life is 8 to 15%. Suicide in the elderly represents an immensely important and often overlooked medical problem. Based on its many distinct features, age-specifi c risk factors could be identifi ed. Proper diagnosis and treatment of affective disorders – contributing greatly to the pathogenesis of suicide in the elderly – could lead to a major decline in the suicide rate in this population.
In this study we investigated the function of p53 as a regulator of cell cycle progression in cycling and senescent cells. Using the conditional temperature-sensitive (ts) mutant we could prevent the detrimental effect of constitutive expression of high levels of wt p53 protein. High levels of wt p53 inhibited cell proliferation by blocking the cells to progress from G1 to S phase of the cell cycle. Flow cytometric analysis revelaed a maintenance of G1 cell population for a longer time depending on the prolonged expression of wt p53 protein. The p53 mediated inhibition of cell proliferation and of the cycle was reversible. However, a spontaneous increase of wt p53 occurring in ageing normal human MRC-5 fibroblasts was associated with irreversible reduction of proliferative potential. The accumulation of G1 cells was detected by flow cytometry. By the measurement of DNA content it is not possible to discriminate between cells arrested in G1 and Go phase, therefore, the expression of G1 markers was determined. Analysis of the expression of distinct cell cycle regulators revealed that quiescent MRC-5 cells were in Go phase. Our results indicate that cell cycle arrest occurring in senescent cells is associated with the Go transition.
To study the detachment stress on the ripeness of strawberry fruit, physiological characteristics of strawberry fruit on and off plant during ripeness and senescence processes were investigated. The results indicated that the ripeness of strawberry fruit upon detachment was accelerated, in terms of firmness, soluble solid content and especially color development. The color of fruit off plant changed rapidly from white to full red in 1–2 days. The respiratory rate in fruit off plant was strengthened, higher than that on plant. Abscisic acid level and ethylene production in fruit off plant were also higher than those on plant and auxin degradation was exacerbated by detachment. Expression levels of FaMYB1, FabHLH3 and FaTTG1 were generally reduced with phenotypes of redder color and more anthocyanin accumulation in fruit off plant. Results also suggested that the detachment initially stimulated ethylene and abscisic acid production and auxin degradation, which modulated ripening-related gene expression and at last enhanced fruit pigmentation.
The effect of exogenous salicylic acid (SA) on the vase life of cut rose flowers was investigated. 'Yellow Island' roses were obtained from a commercial grower. The roses were pulse treated with SA (0, 50, 100, 150, 200 mg l-1) for 18 hours. Com­pared to the control treatment (DW), the greatest delay in senescence was obtained in cut roses treated with 150 mg l-1 SA. With this treatment, flower vase life was dou­bled. The physiological characteristics such as protein concentration, lipid peroxida- tion (MDA) level and enzyme activity such as lipoxygenase (LOX), superoxide dis- mutase (SOD) and peroxidase (POD) were measured only in the control (DW) and those kept in a solution of 150 mg l-1 salicylic acid (SA) for over 8 days of vase life. Degradation of protein and accumulation of MDA during vase life was shown to be suppressed by 150 mg l-1 SA. The activity of LOX and POD gradually increased with progressing flower senescence, while SOD activity declined. The flowers which had been pulsed with SA exhibited lower LOX and POD activity, and a higher activity of SOD. Overall, the results suggest that SA increases vase life by improving the anti- oxidant system and reducing oxidative stress damages during rose flower senescence.
The rate of telomere loss is increasingly being used as a marker of biological aging, organismal senescence, and survival probability. These protective ends of chromosomes act to protect coding DNA during replication and by buffering against degradation from reactive oxygen species (ROS). In many organisms, telomere loss has been linked to increased levels of metabolism, biological stress and disease. Here we validate a medium-throughput and reliable method to measure relative telomere length in Sand Martins Riparia riparia. We performed the qPCR assay on a population of variously aged individuals from eastern Hungary. We detected a significant negative relationship between relative telomere length and age and observed a clear drop in telomere length in older age classes (> 4 years) but no relationship with gender or body mass. Our results in this cross-sectional study support findings in other passerine species that report a lack of long telomeres in older individuals. The method that we describe will allow longitudinal studies of Sand Martin individuals in wild populations to track telomere dynamics related to various life history characteristics and individual health.
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