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The occurrence of spermatozoa with acrosome reaction in semen of boars depending on staining method and storage duration

100%

Wysokinska A., Chlopik A.

Acta Scientiarum Polonorum. Zootechnica

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2019

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tom 18

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nr 4

2

Performance of young boars depending on the litter size

100%

Kawecka M., Jacyno E., Matysiak B., Kolodziej-Skalska A., Kamyczek M.

Acta Scientiarum Polonorum. Zootechnica

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2013

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tom 12

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nr 3

The material consisted of 60 hybrid boars, from line 990 (female) and Pietrain breeds (male), divided into two groups depending on the litter size in which they were born and reared - group: 7-10 piglets (average 8.8) per litter; group: 11-17 piglets (average 12.5) per litter. The mean body weight of 21 day of live piglets born and reared in larger litters was significantly (P < 0.05) lower than among boars reared in smaller litters. This difference gradually diminished and at day 63 of life both groups had the same average body weight. In the period from 63 to 180 day of life, the boars from smaller litters had a slightly lower rate of growth (statistically insignificant). Boars reared in larger litters at 180 day of life were on average 3 kg heavier. There were no significant differences in fat thickness, lean meat and testicular volume between the assessed groups of young boars. From boars reared in smaller litters ejaculates had a significantly higher (P < 0.05) sperm concentration and total sperm volume.

3

Neospora caninum natural infection in Tunisian rams: serological study and molecular identification of infection in semen

100%

Amdouni Y., Rouatbi M., Lassoued N., Rekik M., Gharbi M.

Acta Parasitologica

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2019

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tom 64

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nr 4

4

The effect of breed on freezability of semen of fancy fowl

100%

Siudzinska A., Lukaszewicz E.

Animal Science Papers and Reports

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2008

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tom 26

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nr 4

A simple cryopreservation method described in 1995 by Tselutin et al. was used for freezing the semen of four fancy fowl breeds: White Crested Black Polish (WCBP), Greenleg Partridge (GP),Italian Partridge (IP) and Black Minorca (BP). The differences in quality (ejaculate volume,osmotic pressure, sperm concentration and morphology) of fresh semen between evaluated breeds were observed, as well as the differences in semen freezability. The freezing-thawing process caused significant (P≤0.01) decrease in percentage of live, normal spermatozoa, with coincident increase in percentage of dead spermatozoa and spermatozoa with acrosome defect. In relation to the fresh semen, the number of live, normal spermatozoa that survived cryopreservation procedure constituted 18.1% in WCBP, 25.1% in GP, 26.2% in IP and 33.6% in BM semen.

5

Effects of dietary supplementation with polyunsaturated fatty acids and antioxidants on biochemical characteristics of boar semen

100%

Strzezek J., Fraser L., Kuklinska M., Dziekonska A., Lecewicz M.

Reproductive Biology

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2004

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tom 04

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nr 3

6

Morphology of European brown hare [Lepus europaeus Pallas, 1778] semen

100%

Kozdrowski R., Dubiel A., Dzimira S.

Bulletin of the Veterinary Institute in Puławy

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2006

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tom 50

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nr 3

7

Detection of bovine herpesvirus 1 in bull semen by PCR

100%

Rola J., Zmudzinski J. F.

Bulletin of the Veterinary Institute in Puławy

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1999

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tom 43

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nr 2

The results of PCR were compared with the results of virus isolation test. 44 bovine semen samples were tested. The presence of BHV 1 was demonstrated with the virus isolation test in 6 samples of semen. PCR with external primers detected BHV 1 in 8 samples, while in nested PCR positive amplification was observed in 21 samples. The sensitivity of PCR with external primers and nested PCR were 0.6 and 0.06 of TCID₅₀ respectively.

8

The structure of the spermatozoon of men from Plock and its surroundings

100%

Wilczynska B., Rozwora B., Jaroszewska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2000

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tom 42

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nr 1

9

The influence of semen cryopreservation on the release of some enzymes from Siberian sturgeon [Acipenser baerii] and sterlet [Acipenser ruthenus] spermatozoa

100%

Sarosiek B., Ciereszko A., Rzemieniecki A., Domagala J., Glogowski J.

Archives of Polish Fisheries

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2004

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tom 12

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nr 1

The milt of individual males of Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus) was frozen without cryoprotector (at -79°C) or cryopreserved with methanol as the cryoprotector. The activity of arylsulfatase (AS), acid phosphatase (AcP), β-N-acetylglucosaminidase (NAGase), and protein concentration was determined. The protein concentration and enzymatic activities in supernatant obtained after cryopreservation were higher than in milt plasma, but they were lower than that in the material obtained after freezing at -79°C. The protein and enzymatic leakage of sterlet spermatozoa was statistically higher in supernatants that had been frozen at-79°C than in those that had undergone cryopreservation. Differences in the protein and AS leakage the Siberian sturgeon supernatants were also observed.

10

Changes in the qualities of stallion semen after incubation with Streptococcus zooepidemicus

100%

Danek J., Wisniewski E., Krumrych W., Dabrowska J.

Bulletin of the Veterinary Institute in Puławy

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1996

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tom 40

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nr 2

The influence of ß-haemolytic Streptococcus organisms at concentrations of 100 x, 200 x, and 500 x 10³ cells/ml sperm incubated at 37°C for 60 min with stallion semen suspension on the motility, viability and morphology of the semen, and AspAT activity in seminal plasma was examined. S. zooepidemicus at a concentration of 500 x 10³ cells/ml exhibited a particularly unfavourable effect on semen. All parameters measured altered significantly after a 60 min incubation. The activity of AspAT also changed during a prolonged incubation of sperm with a suspension containing 500 x 10³ S. zooepidemicus/ml semen; after a 24 h incubation the activity of AspAT was enhanced by more than 38% in comparison to the initial value.

11

Seminal plasma proteins as markers of biological value of boar semen

100%

Strzezek J., Saiz-Cidoncha F., Wysocki P., Tyszkiewicz A., Jastrzebski M.

Animal Science Papers and Reports

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2002

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tom 20

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nr 4

12

Estimation of the number of normal spermatozoa in human semen supported by ultrastructural study and the theory of probability

100%

Filipiak K., Butowska W., Warchol J. B.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2000

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tom 42

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nr 1

13

Comparison of egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa

84%

Akhter S., Rakha B. A., Andrabi S. M. H., Ansari M. S.

Animal Science Papers and Reports

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2011

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tom 29

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nr 2

The present study was designed to compare egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa. The study was conducted on cauda epididymal spermatozoa from ten slaughtered Sahiwal bulls. Semen retrieved from epididymal cauda of each testes pair was pooled and diluted in tris-citric acid-glycerol extender containing either pigeon, guinea fowl or chicken egg yolk semi colon leading to a final spermatozoal concentration of 30×106 ml-1. Diluted semen was transferred into 0.5 ml straws, cooled to 4°C and equilibrated for 4 h. The straws were kept on liquid nitrogen vapours for 10 min and then plunged into liquid nitrogen for storage. Motility, plasma membrane integrity and spermatozoa morphology (acrosome ridge;head, mid piece and tail) of each diluted semen sample were assessed at 0, 2 and 4 h post-thawing.All indicators applied and acrosomal integrity were the highest (P<0.05) after freezing in extender containing pigeon egg yolk compared to guinea fowl and chicken egg yolk 0, 2 and at 4 h postthaw.Moreover,spermatozoa tail abnormality percentages were also significantly lower (P<0.05) in extender containing pigeon egg yolk compared to guinea fowl and chicken egg yolk at 0, 2 and 4 post-thaw h. In conclusion, pigeon egg yolk used in semen extender improved the post-thaw quality of Sahiwal bull epididymal spermatozoa.

14

Cryopreservation of bull semen in OptiXcell and conventional extenders: Comparison of semen quality and fertility

84%

Ansari M. S., Rakha B. A., Akhter S.

Animal Science Papers and Reports

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2017

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tom 35

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nr 3

Commercially available OptiXcell® extender was compared with conventional extenders for freezability and in vivo fertility of bull semen. Semen was collected from three Friesian bulls for five weeks (replicate) and qualifying ejaculates (motility >60%, concentration >0.5 billion/mL, volume >lmL) were diluted (37°C; 50 x 10⁶ spermatozoa/ml) with OptiXcell®, tris-citric egg yolk and egg yolk-citrate extenders. Diluted semen was cooled to 4°C in 2 hours, equilibrated for 4 hours and filled in 0.5 ml straws. The straws were kept over liquid nitrogen vapours for 10 minutes and plunged into liquid nitrogen. Percentages of post thaw sperm motility and plasma membrane integrity were recorded higher (P<0.05) in OptiXcell® compared to tris-citric egg yolk followed by egg yolk-citrate extender. Sperm viability (%) were recorded higher (P<0.05) in OptiXcell® compared to tris-citric egg yolk and egg yolk citrate extender. Percentages of normal apical ridge and DNA integrity were higher (P<0.05) in OptiXcell® and tris-citric egg yolk extender compared to egg yolk-citrate extender. Higher (P<0.05) fertility rate was recorded with semen frozen in OptiXcell® compared to triscitric egg yolk and egg yolk-citrate extender. In conclusion, OptiXcell® is superior to conventional extenders for spermatozoa) quality of frozen-thawed bull semen and produced higher fertility rates under field conditions.

15

Effects of semen collection interval on fresh and frozen semen parameters in Tushin rams

84%

Ari U., Lehimcioglu N., Yildiz S., Kulaksiz R., Ozturkler Y.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 1

Ejaculates from three rams were collected with artificial vagina at one day (OD1) and four day intervals (FDsI). Part of the semen samples were pooled, diluted with skim milk based extender: -egg yolk (10%) -glycerol (5%), loaded into 0.25 ml straws, equilibrated, frozen, and afterwards stored in liquid nitrogen. The following spermatozoon parameters were determined in fresh and frozen-thawed semen: motility, viability, abnormality, abnormal acrosome, and membrane integrity. Semen collected at ODI had significantly higher freeze-ability compared with semen collected at FDsI for spermatozoon motility. Frozen-thawed semen collected at ODI had higher viability and membrane integrity, and lower abnormality and abnormal acrosome percentages compared with FDsI. It was concluded that in order to use cryopreservation of the Tushin ram semen, ODI instead of FDsI collection should be preferred.

16

Season's influence on the properties of male domestic pig semen

84%

Kondracki S., Wysokinska A., Kowalewski D., Muczynska E., Adamiak A.

Rozprawy Naukowe. Państwowa Wyższa Szkoła Naukowa im. Papieża Jana Pawła II w Białej Podlaskiej

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2009

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tom 03

A study was carried out of 30, 160 ejaculate samples collected from 81 boars of the wbp (Polish Large White) breed and 182 boars of the pbz (Polish Landrace) breed. The ejaculate was collected manually with the sampling frequency of 4 - 5 days. An assessment of the ejaculate has been done with regard to the following physical properties: ejaculate volume, sperm cells concentration, percentage of sperm cells exhibiting proper motility, total number of sperm cells in the ejaculate and number of insemination doses obtained from one ejaculate. The collected data have been analyzed according to the season criterion with indication of the ejaculate collected in the individual months. It has been revealed that the most suitable ejaculate was collected from the domestic pig boars in November and December whereas in March, April and May the parameters lowered. The ejaculate collected in the springtime is characterized by a lower volume and a lower number of the motile sperm cells than in the other seasons. From the ejaculate collected in the autumn and winter period more insemination doses can be prepared than from those collected in spring and summer. No difference has been found between the boars of the wbp and pbz breeds with regard to their susceptibility to the seasonal factors’ influence.

17

Intrauterine Neospora caninum inoculation using contaminated semen with different numbers of tachyzoites

84%

Serrano E., Ferre I., Osoro K., Aduriz G., Mota R. A., Martinez A., Atxaerandio R., Hidalgo C. O., Ortega-Mora L. M.

Wiadomości Parazytologiczne. Suplement

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2005

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tom 51

18

Boar variability affects sperm metabolism activity in liquid stored semen at 5 degrees Celsius

84%

Dziekonska A., Strzezek J.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

Metabolic activity of boar spermatozoa, liquid stored for three days at 5℃, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5℃ may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.

19

Respiration of bull spermatozoa in presence of exogenous glutathione (GSH)

84%

Slaweta R.

Acta Physiologica Polonica

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1987

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tom 38

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nr 1

20

Motility parameters and intracellular ATP content of rabbit spermatozoa stored for 3 days at 15 degrees Celsius

84%

Gogol P.

Folia Biologica

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2013

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tom 61

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nr 1-2

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