Wyniki wyszukiwania - AGRO

1

Screening of microorganisms for improvement of beta-galactosidase production

100%

Fiedurek J., Ilczuk Z.

Acta Microbiologica Polonica

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1990

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tom 39

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nr 1-2

2

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Screening of cellulase and pectinase by using Pseudomonas fluorescens and Bacillus subtilis

100%

Reetha S., Selvakumar G., Bhuvaneswari G., Thamizhiniyan P., Ravimycin T.

International Letters of Natural Sciences

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2014

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tom 08

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nr 2

A study was conducted to determine the Production of cellulase and pectinase enzyme by using Plant growth promoting rhizobacteria like Pseudomonas fluorescence and Bacillus subtilis. These to micro organism are isolated by serial dilution method. One gram of soil sample was diluted in to 10 ml of sterile distilled water and 1 ml of sample solution was serially diluted in 9ml of sterile water up to 10 dilution. Each sample from dilution 10-5 and 10-6 were taken and streaked in to KB and NA medium and incubate at 24 hrs. After 24 hrs Pseudomonas fluorescence and Bacillus subtilis was observed in the medium of KB and NA medium. Both the culture was sub cultured and maintain in the same for the further work. CMCase medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria and incubates for 48h after incubation period a clear halo zone was produced by these bacteria among these bacteria Pseudomonas fluorescence are able to produce high amount of cellulose compare to Bacillus subtilis. Pectin agar medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria incubates for 48h after incubation period a clear halo zone was produced by these bacteria, among these bacteria Pseudomonas fluorescence are able to produce high amount of Pectinase compare to Bacillus subtilis. Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms.

3

The fungus Penicillium notatum 1, a new source of dextranase

100%

Szczodrak J., Pleszczynska M., Fiedurek J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1994

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tom 42

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nr 2

4

NMR as a method for screening of fatty acids composition in edible oils

86%

Castejon D., Mateos-Aparicio I., Herrera A., Cambero M. I.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

5

Screening of the antioxidant capacity of selected spices by updated analytical strategies

86%

Przygodzka M., Zielinska D., Ciesarova Z., Zielinski H.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

6

The screening of microorganisms capable of methyl tert-butyl ether (MTBE) biodegradation

86%

Wieczorek A., Przybulewska K., Karpowicz K., Nowak M. J.

Polish Journal of Microbiology

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2013

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tom 62

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nr 2

As a result of examinations carried out, 16 strains of microorganisms able to grow on mineral media with methyl tert-butyl ether as the sole source of carbon and energy were isolated. Bacteria prevailed among the isolated microorganisms . The growth of microorganisms under laboratory conditions was long and accompanied by low biomass increase. Under the conditions of the experiment, the isolated microorganisms did not show any quantitatively measurable biodegradability of methyl tert-butyl ether (MTBE) under aerobic conditions. This requires far-reaching caution with respect to trading in MTBE-modified petrols in order to protect the natural environment in Poland against contamination with that hard-to-biodegrade substance.

7

Effects of inoculum potential on screening for resistance to Plasmodiophora brassicae in greenhouse trials

86%

Robak J., Gabrielson R. L.

Acta Agrobotanica

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1988

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tom 41

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nr 2

Several factors, including growth medium, inoculum density, and inoculum storage affected the reaction of resistant and susceptible Brassicas to Plasmodiophora brassicae in the greenhouse. A high level of disease was achieved using Peat-litte mix R and a commercial greenhouse mix. There was little difference in disease incidence when spore suspensions were pipeted into planting holes or when seedlings were dipped into spore suspensions. Seedlings transplanted from sand or Petri dishes gave higher levels of disease than direct seeding. Two-year frozen storage of clubs reduced the inoculum potential to a level unable to define resistance. Inoculum levels of 10³⁻⁷ spores per ml from fresh clubs, or 10⁵⁻⁷ spores per ml from clubs frozen for 2 or 4 years, produced 90% club incidence of susceptible cauliflower and Chinese cabbage. A concentration of only 10⁶⁻⁸ spores per ml from fresh clubs was required for maximum disease expression in a cauliflower line partially resistant to clubroot.

8

Easy PCR screening of Pichia pastoris transformants

86%

Arora D., Chauhan A., Khanna N.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 1

A simple protocol for easy PCR screening of P. pastoris transformants is described. In short, the P. pastoris cells are lysed with very small amount of the enzyme Zymolase and the crude cell lysate is directly used in PCR. This protocol needs no tube transfer steps and also obliviates the requirement of freezing the samples at -80°C before PCR screening. Because of a single step screenig, both overall and actual hands on time are considerably reduced.

9

Molecular methods for rapid detection of aneuploidy

86%

Dudarewicz L., Holzgreve W., Jeziorowska A., Jakubowski L., Zimmermann B.

Journal of Applied Genetics

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2005

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tom 46

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nr 2

Rapid molecular biological methods for prenatal diagnosis of the most common aneuploidies, collectively known as rapid aneuploidy testing, are compared in this review. We discuss methodological problems and limitations of these various methods. All these techniques are believed to be accurate and carry a low risk of misdiagnosis, but they differ in terms of labour-intensity and amenability to automation and high throughput testing. The question how to apply them safely and economically in a clinical setting has not been answered yet. The discussed techniques are so far not used as stand-alone tests, but some of them are routinely applied as a preliminary test that shortens the waiting time for classic cytogenetic karyotyping. In the future, mainly because of economical reasons, these methods may replace cytogenetics in the category of patients who make up the majority of those currently offered prenatal karyotyping: patients with moderately increased risk and no abnormalities detected by ultrasound.

10

A study on mass health screening for prevention of osteoporosis

86%

Tatsumi M., Onoda T., Nohara M., Itai K., Tsunoda H.

Annals of Agricultural and Environmental Medicine

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1994

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tom 01

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nr 2

11

Evaluation of two techniques for screening tomatoes for resistance to Fusarium crown and root rot

86%

Kozik E. U.

Vegetable Crops Research Bulletin

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1999

|

tom 50

12

Evaluation of periostin in myocardial infarction in a mouse model - preliminary results of microarray screening

72%

Fil D., Niklinska W., Mackiewicz Z.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

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nr 1

13

Universal screening as a recommendation for thyroid tests in pregnant women

72%

Matuszek B., Zakoscielna K., Baszak-Radomanska E., Pyzik A., Nowakowski A.

Annals of Agricultural and Environmental Medicine

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2011

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tom 18

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nr 2

14

Genotypic diversity, antimicrobial resistance and screening of Vibrio cholerae molecular virulence markers in Vibrio alginolyticus strains recovered from a Tunisian Ruditapes decussatus hatchery

72%

Mechri B., Medhioub A., Medhioub M. N., Aouni M.

Polish Journal of Microbiology

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2013

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tom 62

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nr 3

In this study, a total of 54 Vibrio alginolyticus strains were analyzed. The isolates were recovered from different compartments of the Ruditapes decussatus hatchery in the National Institute of Marine Sciences and Technologies, Monastir, Tunisia. All isolates were biochemically identified (API 20E and API ZYM strips), characterized by amplification of the Hsp-40 gene polymerase chain reaction (PCR) and analyzed by enterobacterial repetitive intergenic consensus (ERIC)-based genotyping to evaluate genetic relationship between the isolated strains. We also looked for the presence of ten V. cholera virulence genes (toxRS, toxR, toxT, toxS, tcpP, tcpA, ace, vpi, zot and ctxA) in the genomes of Vibrio isolates. The antibiotics susceptibility, exoenzymes production and in vitro cytotoxic activitiy against HeLa cell line were also carried out for all tested bacteria. Most of V. alginolyticus isolates showed significant antimicrobial resistance rates to at least ten antibacterial agents. For most isolates, the minimum inhibitory concentration (MIC) data showed that tetracyclin and streptomycin were the most effective antibiotics. Construction of the phylogenetic dendogram showed that studied isolates were in general genetically heterogeneous; however some Vibrio strains were present in different structures of the R. decussatus hatchery. The V. cholerae virulence genes investigation showed a wild distribution of toxS (49/54), toxR (45/54) and toxT (22/54) genes among V. alginolyticus strains isolated from the R. decussatus rearing system. Cytotoxic effects of several Vibrio extracellular products (28/54) were also observed on HeLa cells.

15

Lactococcus lactis IBB477 presenting adhesive and mucoadhesive properties as a candidate carrier strain for oral vaccination against influenza virus

72%

Radziwill-Bienkowska J. M., Zochowska D., Bardowski J., Mercier-Bonin M., Kowalczyk M.

Acta Biochimica Polonica

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2014

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tom 61

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nr 3

16

Epilithic biofilms in Lake Baikal: screening and diversity of PKS and NRPS genes in the genomes of heterotrophic bacteria

72%

Sukhanova E., Zimens E., Kaluzhnaya O., Parfenova V., Belykh O.

Polish Journal of Microbiology

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2018

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tom 67

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nr 4

A collection of heterotrophic bacteria consisting of 167 strains was obtained from microbial communities of biofilms formed on solid substrates in the littoral zone of Lake Baikal. Based on the analysis of 16S rRNA gene fragments, the isolates were classified to four phyla: Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. To assess their biotechnological potential, bacteria were screened for the presence of PKS (polyketide synthase) and NRPS (non-ribosomal peptide synthetases) genes. PKS genes were detected in 41 strains (25%) and NRPS genes in 73 (43%) strains by PCR analysis. The occurrence of PKS genes in members of the phylum Firmicutes (the genera Bacillus and Paenibacillus) was 34% and NRPS genes were found in 78%. In Proteobacteria, PKS and NRPS genes were found in 20% and 32%, and in 22% and 22% of Actinobacteria, respectively. For further analysis of PKS and NRPS genes, six Bacillus and Paenibacillus strains with antagonistic activity were selected and underwent phylogenetic analysis of 16S rRNA genes. The identification of PKS and NRPS genes in the strains investigated was demonstrated among the homologues the genes involved in the biosynthesis of antibiotics (bacillaene, difficidine, erythromycin, bacitracin, tridecaptin, and fusaricidin), biosurfactants (iturin, bacillomycin, plipastatin, fengycin, and surfactin) and antitumor agents (epothilone, calyculin, and briostatin). Bacillus spp. 9А and 2А strains showed the highest diversity of PKS and NRPS genes. Bacillus and Paenibacillus strains isolated from epilithic biofilms in Lake Baikal are potential producers of antimicrobial compounds and may be of practical interest for biotechnological purposes.

17

Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol

72%

Dabrowski S., Zablotna E., Pietrewicz-Kubicz D., Dlugolecka A.

Acta Biochimica Polonica

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2012

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tom 59

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nr 3

Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.

18

The influence of screening process parameters on paper properties produced from wastepaper

72%

Nikalaichyk A., Malachowska E.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2020

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tom 110

19

Preliminary assessment of usefulness of cELISA test for screening pig and cattle populations for presence of antibodies against Toxoplasma gondii

72%

Sroka J., Karamon J., Cencek T., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2011

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tom 18

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nr 2

20

Screening of different extracts of marine macro green algae for larvicidal activity against dengue fever mosquito, Aedes aegypti (diptera: Culiadae)

72%

Adaikala Raj G., Jayaraman M., Krishnamoorthy S., Chandrasekaran M., Venkatesalu V.

International Letters of Natural Sciences

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2017

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tom 62

The present study larvicidal activities of hexane, chloroform, ethyl acetate, acetone and methanol extracts of Halimeda macroloba, Decsne, Caulerpa racemosa (Frosk) Weber-Van-Bosses and Ulva lactuca Lin, (Chlorophyceae) against Aedes aegypti. The marine macro green algae extracts were tested against early 4 th instar larvae of A. aegypti using WHO protocol and concentrations of 200, 400, 600,800 and 1000 ppm. The observed mortality was made 24 and 48 h after treatment, data was subjected to probit analysis to determine the lethal concentration (LC₅₀ and LC₉₀) of the treated larvae of the tested species. Among the tested extracts the maximum efficacy was observed in the ethyl acetate extracts. The ethyl acetate extracts of the seaweeds showed the presence of terpenoids, tannins and phenolic compounds stronger than the other extracts. The results revealed that all the extracts showed varied levels of larvicidal activity against A. aegypti tested. However, the ethyl acetate extract of C. racemosa showed remarkable larvicidal activity against A. aegypti (LC₅₀=579.9 and LC₉₀=1255.4 ppm values at 24 h and LC₅₀=495.4 and LC₉₀=1073.9 ppm at 48 h) followed by U. lactuca (LC₅₀=588.1 and LC₉₀=1290.7 ppm values at 24 h, and LC₅₀= 530.8, and LC₉₀= 1160.0 ppm at 48 h), respectively. The lowest larval mortality was observed with hexane extract of H. macroloba against A. aegypti with values of LC₅₀=1116.8 and LC₉₀= 1824.5 ppm (after 24 h) and LC₅₀=1059.9 and LC₉₀=1768.3 ppm (after 24 h). The present studies indicate that the larvicidal activity and phytochemicals derived from the ethyl acetate extract of C. racemosa have the potential to be used as an ideal eco-friendly approach and effective mosquito vector control agent.

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