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Systematic research on house dust mites in the Czech Republic started in the early seventies, when mite fauna of human dwellings and distribution of pyroglyphid mites were studied. Special attention was paid to old-age pensioners' homes and children's sanatoria. Pyroglyphidae were found also in the city air and in the dust of city pavements, especially in localities with high occurrence of domestic pigeons. While D. pteronyssinus and D. farinae mostly occurred in permanently inhabited flats, Euroglyphus maynei prevailed in recreation houses and some hospitals. Autumn increase of population density occurred in both, D. pteronyssinus and D. farinae populations, however, the number of protonymphs was remarkable higher than that of other developmental stage in D. farinae population. Special rearing technique was developed for the mass-culture of mites and a Czechoslovak patent was assigned to the technology of allergen preparation for the diagnostics and the desensibilization of patients with house-dust allergies.
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Alergia na roztocze kurzu domowego

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There are presented the etiology and pathogenesis of house dust and house dust mite allergy. The house dust mite ecology, physicochemic and immunochemic properties of house dust mite allergens, methods of quantitating exposure to these allergens; the relationship between level of exposure, sensitization and disease, likewise avoidense measures for mite allergens in houses are described.
The residues of fluvalinate accumulated in the wax promote the selection and development of Varroa jacobsoni resistance to fluvalinate activity. Probably Varroa jacobsoni will be resistant to other acaricidal synthetic pyrethroids (due to cross-resistance). The possibilities of using for honey-bee protection the inserted to hives strips, containing known pesticides, such as amitraz, malathion and bromfenvinphos, which have different, than synthetic pyretroids, mode of biological action, and which are suitable to overcome fluvalinate resistant mites were tested in Poland. The high varroacidal effectiveness of the strips with above mentioned pesticides during 6 weeks exposition was found. However, amitraz, at the concentration of 5% in PVC strip, was decomposed after 5 months of storage. Malathion at the same concentration is not quite safe for bees. The promising results with bromfenvinphos incorporated in PVC strip (5%), as well as in the form of microlayer (50-60 mg a.i.) on PE strip were obtained (the average varroacidal effectiveness achieved 99%). The dorsal bromfenvinphos apitoxicity LD50 was 7310 ng/bee.
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