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The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.

100%

Grzegorczyk I., Bilichowski I., Mikiciuk-Olasik E., Wysokinska H.

Acta Societatis Botanicorum Poloniae

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2006

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tom 75

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nr 1

11-15

This report describes the effect of triacontanol on shoot multiplication and production of antioxidant compounds (carnosic acid, carnosol and rosmarinic acid) in S. officinalis cultures grown on MS basal medium (agar solidified medium supplemented with 0.1 mg l-1 IAA, 0.45 mg l-1 BAP). It was found that shoot proliferation significantly increased when triacontanol at concentrations of 5, 10 or 20 µg l-1 was added to the medium. HPLC analysis of acetone and methanolic extracts of sage shoots showed that the production of diterpenoids, carnosic acid/carnosol ratio, as well as, contents of rosmarinic acid were also affected by the treatment with triacontanol. The highest stimulation effect of triacontanol was observed on the production of carnosol, where the treatment with 20 µg l l-1 increased the content of this diterpenoid 4.5-fold compared to that in the control (sage shoots growing on MS basal medium, only).

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The effect of methyl jasmonate on production of antioxidant compounds in shoot cultures of Salvia officinalis L.

100%

Grzegorczyk I., Wysokinska H.

Herba Polonica

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2009

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tom 55

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nr 3

238-243

The shoots of Salvia officinalis growing in MS liquid medium supplemented with IAA 0.1 mg l-1) and BAP (0.45 mg l-1) were treated with methyl jasmonate (MeJA) to increase production of compounds with antioxidant activity (carnosic acid, carnosol and rosmarinic acid). The increase in metabolite production depended on MeJA concentration, the period of exposure to elicitor and type of compound. The MeJA action was observed 24 h after elicitation. It was found that the maximum level of diterpenoids, calculated as the sum of CA and Car (about 8 mg g-1 dry wt) was achieved at 3 days after elicitation with 20 µM methyl jasmonate. The highest amount of rosmarinic acid (about 41 mg g-1 dry wt) was achieved with 50 or 100 μM methyl jasmonate on the 5th day after elicitation. It was almost 2-fold higher compared to the control (cultures treated with only ethanol).

3

Effects of rosmarinic acid on cognitive and biochemical alterations in ovariectomized rats treated with D-galactose

100%

Gok D. K., Ozturk N., Er H., Aslan M., Demir N., Derin N., Agar A., Yargicoglu P.

Folia Histochemica et Cytobiologica

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2015

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tom 53

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nr 4

4

Identification of bioactive polyphenolic compounds and assessment of antioxidant activity of Origanum acutidens

100%

Aras A., Silinsin M., Bingol M. N., Bursal E.

International Letters of Natural Sciences

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2017

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tom 66

The main objective of the current work is to search the antioxidant activities of Origanum acutidens leaves by using DPPH, FRAP and CUPRAC methods severally. Three well known antioxidant compounds (BHA, BHT and ascorbic acid) were used as standards for comparing the extracts. Also, phenolic compounds of Origanum acutidens leaves were identified by UHPLC-ESI-MS/MS. The high concentrations of rosmarinic acid (11158.99 ppb) quinic acid (3200.84 ppb) and naringenin (1238.45 ppb) were detected quantitatively.

5

Potential sources of rosmarinic acid

100%

Modnicki D., Matlawska I.

Herba Polonica

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2007

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tom 53

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nr 2

6

In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds

100%

Grzegorczyk I., Bilichowski I., Mikiciuk-Olasik E., Wysokinska H.

Acta Societatis Botanicorum Poloniae

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2005

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tom 74

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nr 1

The concentrations of carnosic acid, carnosol and rosmarinic acid in different materials from differentiated (multiple shoot cultures and regenerated plants) and undifferentiated (callus and cell suspension) in vitro cultures of Salvia officinalis were determined by HPLC. The results suggested that diterpenoid (carnosic acid and carnosol) production is closely related to shoot differentiation. The highest diterpenoid yield (11.4 mg g-1 for carnosic acid and 1.1 mg g-1 for carnosol) was achieved in shoots of 10-week-old micropropagated plants. The levels were comparable to those found in shoots of naturally growing plants. Undifferentiated callus and cell suspension cultures produced only very low amounts of carnosol (ca. 0.05 mg g-1 of dry weight). In contrast, content of rosmarinic acid in callus and suspension cultures as well as shoots growing in vitro and in vivo was similar and ranged between 11.2 and 18.6 mg g-1 of dry weight.

7

Determination of spasmolytic and antispasmodic activities of thyme extracts and one of their major components, rosmarinic acid, in isolated rabbit ileum and isolated rat aorta

80%

Bazylko A., Zygmunt M., Sapa J., Strzelecka H., Filipek B.

Acta Biologica Cracoviensia. Series Zoologia

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2009

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tom 51

49-54

8

Zwiazki o wlasciwosciach przeciwutleniajacych w roslinach z rodzaju Salvia

80%

Grzegorczyk I., Kuzma L., Wysokinska H.

Bromatologia i Chemia Toksykologiczna

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2004

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tom 37

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nr 3

209-216

9

Accumulation of biologically active compounds in Eleuthero [Eleutherococcus senticosus-Rupr. et Maxim.-Maxim.] grown in Poland

80%

Baczek K.

Herba Polonica

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2009

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tom 55

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nr 1

7-13

Eleuthero (Eleutherococcus senticosus /Rupr. et Maxim./ Maxim.) is a shrub native to north-eastern Asia. Underground parts of this plant are classified as a drug with adaptogenic activity. Eleutherosides are regarded as main active compounds of this plant. The studies on cultivation and raw material quality of eleuthero were undertaken in the Department of Vegetable and Medicinal Plants of Warsaw University of Life Sciences – SGGW. The content of eleutherosides B and E and phenolic acids in the raw materials were determined by HPLC. The total content of eleutherosides B and E in the dried rhizomes with roots exceeded considerably the content of these compounds required by the British Pharmacopoeia (80 mg/100 g). In the raw material of two-, three- and four-year-old plants it amounted to 104.7, 167.4 and 292.4 mg/100 g, respectively. The content of eleutherosides in the bark of woody stems was almost twice as high as the content of these compounds in the rhizomes with roots. Both rhizomes with roots and bark of woody stems were characterised by high content of phenolic acids, especially chlorogenic acid (849.8 and 421.0 mg/100g, respectively).

Ograniczanie wyników

The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.

The effect of methyl jasmonate on production of antioxidant compounds in shoot cultures of Salvia officinalis L.

Effects of rosmarinic acid on cognitive and biochemical alterations in ovariectomized rats treated with D-galactose

Identification of bioactive polyphenolic compounds and assessment of antioxidant activity of Origanum acutidens

Potential sources of rosmarinic acid

In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds

Determination of spasmolytic and antispasmodic activities of thyme extracts and one of their major components, rosmarinic acid, in isolated rabbit ileum and isolated rat aorta

Zwiazki o wlasciwosciach przeciwutleniajacych w roslinach z rodzaju Salvia

Accumulation of biologically active compounds in Eleuthero [Eleutherococcus senticosus-Rupr. et Maxim.-Maxim.] grown in Poland