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1

An approach to cloning of Pi-b rice blast resistance gene

100%

Rybka K., Miyamoto M., Nakamura S., Kawasaki S.

Acta Physiologiae Plantarum

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1997

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tom 19

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nr 4

A contig of clones from BAC rice genomic library encompassing blast resistance gene Pi-b was constructed. On an average eight clones (8 ± 2.6) were picked up by each marker, which was expected basing on the BAC library size (Nakamura et al. 1997). The 2.4 cM distance between flanking RFLP markers G 1234 and RZ 213 (Miyamoto et al. 1996) was spanned with 4 steps of contig including 25 clones. The physical distance of 370 kb between flanking markers corresponds to a small ratio of physical and genetical distances (155 kb/cM) due to a probable structure of the gene locus near the telomeric end of the chromosome. Markers cosegregating with blast resistance against Magnoporthe grisea were localized in a 2 kb restriction fragment. A new border marker was found on the telomeric side of the Pi-b gene, less than 10 kb from cosegregating markers. No clear marker for the centromeric side of the gene was found but the position of Pi-b rice blast resistant gene was narrowed to within at least 50 kb, which is to our knowledge the most precised estimation of the position of this gene.

2

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Genes for resistance to wheat powdery mildew

100%

Chen Y., Chelkowski J.

Journal of Applied Genetics

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1999

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tom 40

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nr 4

3

Mapping of Pi-b rice blast [Magnoporthe grisea] resistance gene

100%

Rybka K., Kawasaki S.

Biological Bulletin of Poznań. Supplement

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1997

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tom 34

4

Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

86%

Al-Daej M. I., Ismail M., Rezk A. A., El-Malky M. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 3

5

Virulence changes in wheat leaf rust in Hungary

86%

Manninger K.

Genetica Polonica

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1994

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tom 35B

6

Resistance of spring wheat cultivars and lines to leaf rust

86%

Wisniewska H., Stepien L., Kowalczyk K.

Journal of Applied Genetics

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2003

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tom 44

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nr 3

7

Resistance gene analogues of Arabidopsis thaliana: recognition by structure

86%

Chelkowski J., Koczyk G.

Journal of Applied Genetics

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2003

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tom 44

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nr 3

8

Antibiotic resistance genes and virulence factors in Enterococcus faecium and Enterococcus faecalis from diseased farm animals: pigs, cattle and poultry

72%

Seputiene V., Bogdaite A., Ruzauskas M., Suziedeliene E.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 3

Eighty enterococcal isolates (E. faecium, n=38, E. faecalis, n=42) from diseased farm animals (swine, cattle, poultry) in Lithuania have been studied for the prevalence of antibiotic resistance and for resistance and virulence genetic determinants. 86% of E. faecium and 71% of E. faecalis isolates were multidrug resistant (resistant to three or more unrelated antibiotics). Resistance to aminoglycosides, tetracycline and erythromycin was found most frequently in both species (61%, 69%) and was linked to aph(3’)-IIIa, aac(6’)-Ie-aph(2”)-Ia, ant(6)-Ia (aminoglycoside resistance), tetM, tetL (tetracycline resistance), ermA, ermB (erythromycin resistance) gene combinations, which were supplemented with chloramphenicol resistance genes catA7, catA8 (E. faecalis) and catA9 (E. faecium). All E. faecalis isolates harboured genes coding for virulence factors agg, esp, fsr gelE alone or in combinations with the high prevalence of esp gene in isolates from cattle (63%) and pigs (79%). The origin- dependent incidence of agg gene variants prgB and asp1 was observed. The results indicate the existence of a large pool of potentially virulent and multidrug resistant E. faecalis in diseased farm animals posing risk to humans.

9

Evaluation of grapevine resistance to downy and powdery mildew in ‘Regent’ × ‘Bogazkere’ hybrid population segregating for resistance genes

72%

Shidfar M., Akkurt M., Atak A., Ergul A., Soylemezoglu G.

Acta Scientiarum Polonorum. Hortorum Cultus

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2019

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tom 18

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nr 1

Fruit production should be adapted to future scenarios that are frequently associated with scarce resources, especially freshwater and fertilizers. New biologically-based fruit production strategies, i.e. taking into account tree growth and water status, are required to optimize irrigation and fertilization under abiotic stress conditions. It was hypothesized that a moderate abiotic stress, here deficit irrigation with or without nitrogen deficit, in the preharvest period, could decrease postharvest losses due to diseases and pruning weights due to reduced vegetative growth, without sacrificing the yield and fruit quality. This study was conducted over two years using the same trees of ‘Moncante’ nectarine cultivar grown in a commercial orchard. Trees were assigned to three treatments: (1) full irrigation at 80% estimated crop evapotranspiration (ETc), (2) deficit irrigation, i.e. at 75% of full irrigation, and (3) deficit irrigation and deficit nitrogen, i.e. at 75% of full irrigation and 75% of usual N-fertilization adopted by the grower in this commercial orchard. Deficit irrigation alone and in combination with deficit nitrogen reduced postharvest diseases and pruning weights without significant yield losses. Our results suggest that ETc-based approaches of reduced water irrigation may be a sustainable way to decrease phytosanitary inputs and workload in the orchard while maintaining the orchard performance.

10

Odporność na mączniaka prawdziwego (Blumeria graminis f. sp. hordei) odmian jęczmienia włączonych do badań rejestrowych w Polsce w roku 2012

72%

Czembor J. H., Doraczynska O., Pietrusinska A., Czembor H. J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2013

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nr 268

11

Odporność na mączniaka prawdziwego (Blumeria graminis f.sp. hordei) odmian jęczmienia włączonych do badań rejestrowych w Polsce w roku 2011

72%

Czembor H. J., Czembor J. H., Pietrusinska A., Domeradzka O.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2012

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nr 265

12

Developing of DNA-marker to the Fusarium oxysporum f.sp. lycopersici resistance genes of tomato

72%

Amini J.

Journal of Plant Protection Research

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2009

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tom 49

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nr 2

175-178

Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici is a destructive disease of tomato crops worldwide. The use of resistant varieties is the best strategy for disease control. In the present study we analyze eight tomato lines and hybrids for Fusarium wilt disease resistance by polymerase chain reaction. Total genomic DNA was extracted from young leaves of three-week-old plants of tomato. Results of PCR of eight tomato lines and hybrids indicated that there are one dominant heterozygote, two recessive homozygotes and five dominant homozygotes. Also, results of polymerase chain reaction showed that it needs less time and is cheaper. Also by using this method, it is possible to determine genotype of plant (homozygote or heterozygote) without presence of the pathogen. Therefore, PCR technique was used in the identification gene I2 conferring resistance to F. oxysporum f. sp. lycopersici.

13

Effectiveness of Puccinia recondita f. sp. tritici resistance genes at different phenological stages of winter wheat

72%

Liatukas Z., Ruzgas V.

Journal of Plant Protection Research

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2006

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tom 46

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nr 2

121-131

The resistance of winter wheat varieties to Puccinia recondite f. sp. tritici was investigated at the Lithuanian Institute of Agriculture during 2001–2003. Effectiveness of resistance genes was investigated at seedling, tillering and adult plant stages. Virulence tests done during the 2000–2003 period showed that the majority of Lr genes used in European wheat were not sufficiently efficient. However, testing of cultivars at the first leaf stage revealed that the Lr37 gene in combination with the other genes was very effective. The experimental cultivars were sown in 2 times: in autumn and spring, without vernalization. The main task of spring-sown nursery was to improve the effectiveness of the experiment and investigate the effect of different Lr genes of non-vernalized plants at tillering growth stage. The Lr37 gene was found to be the most effective at both adult plant stage and tillering growth stage. Disease severity and plant resistance type at tillering stage were stable in all experimental years, which is important for the breeding program. The investigations revealed that the correlations between resistance at seedling and the other two stages were up to r = 0.81 (significant at p = 0.01**). The correlations between leaf rust severity and varietal resistance type at tillering were very high (r = 0.86–0.91**) in the same year. The correlation of leaf rust severity at adult plant stage was strong (r = 0.78**) between 2001 and 2002, but too low for reliable selection of resistant cultivars in the other years. Spring-sown nursery was complementary to collect resistance data in the years unfavourable for leaf rust development.

14

Leaf rust resistance genes of wheat: identification in cultivars and resistance sources

72%

Stepien L., Golka L., Chelkowski J.

Journal of Applied Genetics

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2003

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tom 44

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nr 2

15

Application of STS markers for leaf rust resistance genes in near-isogenic lines of spring wheat cv. Thatcher

72%

Chelkowski J., Golka L., Stepien L.

Journal of Applied Genetics

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2003

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tom 44

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nr 3

16

Powdery mildew resistance genes in wheat: verification of STS markers

72%

Stepien L., Chen Y., Chelkowski J., Kowalczyk K.

Journal of Applied Genetics

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2001

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tom 42

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nr 4

17

Molecular markers for leaf rust resistance genes in wheat

72%

Chelkowski J., Stepien L.

Journal of Applied Genetics

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2001

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tom 42

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nr 2

18

DNA polymorphism among barley NILs of cv. Pallas, carrying genes for resistance to powdery mildew [Blumeria graminis f.sp.hordei]

72%

Czembor P. C., Czembor J. H.

Journal of Applied Genetics

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2004

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tom 45

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nr 2

19

An assessment of the resistance gene analogues of Oryza sativa ssp.japonica: their presence and structure

72%

Koczyk G., Chelkowski J.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 4

Rice is the first cereal genome of known draft sequence, and the finished sequence for it is now nearly complete. In this paper, we describe a preliminary analysis of known rice genes aimed to detect resistance gene analogues of known structural classes. Putative resistance genes were identified in a dual approach - by using BLASTP searches to identify candidate sequences and by using Hidden Markov Models to predict domain presence in the candidates. The set of proteins examined was obtained from the publicly available data of TIGR (The Institute for Genomic Research). 1744 distinct RGAs were identified, 597 of which belonged to the NBS-LRR class. Supplementary data (sequences and annotations) is available on the web site http://gkoczyk.bioinfo.pl/CMBL.

20

Resistance genes in barley [Hordeum vulgare L.] and their identification with molecular markers

72%

Chelkowski J., Tyrka M., Sobkiewicz A.

Journal of Applied Genetics

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2003

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tom 44

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nr 3

Ograniczanie wyników

An approach to cloning of Pi-b rice blast resistance gene

Genes for resistance to wheat powdery mildew

Mapping of Pi-b rice blast [Magnoporthe grisea] resistance gene

Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

Virulence changes in wheat leaf rust in Hungary

Resistance of spring wheat cultivars and lines to leaf rust

Resistance gene analogues of Arabidopsis thaliana: recognition by structure