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The article reviews the issue of antibiotic resistance of microorganisms to meropenem in intensive care units in Ukraine. An increase in meropenem inefficiency against microorganisms in intensive care units has been observed in the last years. The data analysis suggests a significant predominance of gram-negative flora: A. baumannii, Р. aeruginosa, К. pneumoniae, E. cloacae, E.coli. On average 30% of microorganisms are resistant to 5 and more basic antibiotics including meropenem. 40-80 % of the gram-negative flora in intensive care units in Ukraine are resistant to meropenem. This can be attributed to the free sale of antibiotics without prescription, patients’ self-treatment, inadequate antibiotic therapy, and failure to comply with sanitary norms on the part of intensive care staff. Microbiological diagnostics of infectious pathogens also needs improvement. Unless proper measures are taken within a few years, meropenem as an antibiotic is likely to disappear in Ukraine.
The inheritance of resistance to loose smut (Ustilago nuda) in seven cultivars of spring barley has been examined. The performed studies showed that, resistance to two different groups of U. nuda races in respect of their virulence is determined by a single allele pair in the cvs. Anoidium and Inerme 2-r and by two allele pairs in the cvs. CI 13 662, Dorsett, Jet and OAC 21. In the cv. Abyssinian, resistance to a group of races 2 is determined by a single allele pair, whereas that to a group of races 4 - by two allele pairs. In all studied cultivars (except Anoidium) the resistance dominates over sensitivity. Resistance to the both studied groups of U. nuda races is determined by similar genes in the cvs. Dorsett and CI 13 662, as well as in Dorsett and OAC21, and additionally to a group of races 4 in the cvs. Abyssinian and OAC 21. No similarity was found between resistance genes in the case of two allele pairs in the cvs. Jet, Abyssinian and CI 13 662 (group of races 4) as well as in Jet, Dorsett and OAC 21 (in both groups of races), and in the case of a single allele pair in the cvs. Inerme-2-rowed and Abyssinian (group races 2).
A pot experiment was carried out to determine the effect of soil (loamy sand and sandy loam) contamination with copper doses of 0, 150, 450 mg Cu·kg-1 d.m. soil on the activity of β-glucosidase (EC 3.2.1.21), acid phosphatase (EC 3.1.3.2), alkaline phosphatase (EC 3.1.3.1) and arylsulfatase (EC 3.1.6.1) in soil. The resistance of these enzymes to copper pollution was also estimated. Soil samples were contaminated with copper chloride. The experiment was carried out in five replications, in two series. The first series was performed on uncropped soil and the second one — on cropped soil. The experimental plants were oat, spring rape and yellow lupine. The activity of soil enzymes was determined in the analyzed samples on the 25th and the 50th day of the experiment. The results of the experiment showed that copper contamination in doses of 150 mg to 450 mg·kg-1 soil significantly inhibits soil’s biochemical activity. The sensitivity of the tested enzymes to copper was determined in the following order: alkaline phosphatase > arylsulfatase > acid phosphatase > β-glucosidase. The resistance of the above enzymes to copper depended on the cultivated plant spe- cies, soil type and the type of soil use and management. In samples of sandy loam, copper induced the smallest change in the activity of acid phosphatase and alkaline phosphatase, and in loamy sand — in the activity of arylsulfatase and acid phosphatase. In uncropped soil, copper was the least effective in changing the activity of arylsulfatase and acid phosphatase. All of the tested enzymes were less resistant to copper contamination in cropped than in uncropped soil. In soil planted with oat, β-glucosidase was the most resistant and arylsulfatase was the least resistant enzyme to copper contamination. In samples sown with spring rape, the analogous enzymes were arylsulfatase and alkaline phosphatase. In yellow lupine treatments, alkaline phosphatase was the most and β-glucosidase was the least resistant enzyme.
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Opportunities and threats in the post-antibiotic era

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The following article reviews the issue of antibiotic resistance of microorganisms to meropenem in intensive care units in Ukraine. An increase in meropenem inefficiency against microorganisms in intensive care units has been observed in the last years. The data analysis suggests a significant predominance of gram-negative flora: A. baumannii, Р. aeruginosa, Antimicrobial resistance happens when microorganisms change when they are exposed to antibiotics. Then, treatment becomes ineffective and infections persist in the body, increasing the risk of spreading to other persons. The new resistance mechanisms that are emerging and spreading globally cause that the so far applied methods of treatment do not work, threatening the human ability to resist common infectious diseases, which in turn results in prolonged infections or even death. Antimicrobial resistance occurs naturally over time, usually through genetic changes. However, the misuse and overuse of antimicrobials are accelerating this process. It has become common to overuse and misuse antibiotics both in people and animals, which are often prescribed without professional oversight. Antimicrobial resistance is a complex problem that affects all of society and is driven by many interconnected factors. Single, isolated interventions have limited impact. Coordinated action is required to minimise the emergence and spread of antimicrobial resistance.
Several methods were evaluated in an attempt to develop a greenhouse screening procedure that would predict field resistance of brassica breeding lines to clubroot disease caused by Plasmodiophora brassicae. Several Brassica oleracea cultivars and breeding lines bred for resistance to Plasmodiophora brassicae and a susceptible Chinese cabbage cultivar were exposed to high levels of inoculum of both pathotypes PB 6, PB 7 at 12,15,20, 25 and 30°C. No infection occurred on any host at 12°C. Chinese cabbage was heavily diseased from 15 - 30°C. Bagder Shipper cabbage, a cauliflower deriving resistance from this variety, and Oregon CR-1 broccoli were resistant to pathotype PB 6 at 15 and 20°C and partially resistant at 25 and 30°C. They were resistant to pathotype PB 7 and 15°C and almost totally susceptible at 20, 25° and 30°C. Oregon cabbage line OR 123 was resistant to pathotype PB 6 at 15°C at almost completely susceptible at 20, 25 and 30°C. It was resistant to pathotype PB 7 at all temperatures. Temperature sensitivity of resistance can partially explain why breeding lines are resistant in field trials and susceptible in greenhouse tests.
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This review summarizes current data on resistance among Salmonella spp. isolates of food origin from countries in different regions of the world. The mechanisms of resistance to different groups of antimicrobial compounds are also considered. Among strains resistant to quinolones and/or fluoroquinolones the most prevalent mechanism is amino acid substitutions in quinolone resistance-determining region (QRDR) of genes gyrA, parC but mechanism of growing importance is plasmid-mediated quinolone resistance (PMQR) associated with genes qnrA, qnrB, qnrC, qnrD, qnrS but frequency of their detection is different. Resistance to sulfonamides is mostly associated with genes sul1 and sul2, while resistance to trimethoprim is associated with various variants of dhfr ( dfr) genes. Taking into account Salmonella spp. strains isolated from food, resistance to β-lactams is commonly associated with β-lactamases encoding by blaTEM genes. However strains ESBL and AmpC – positive are also detected. Resistance to aminoglicosides is commonly result of enzymatic inactivation. Three types of aminoglycoside modifying enzyme are: acetyltransferases (AAC), adenyltransferases (ANT) and phosphotransferases (APH). Resistance to tetracyclines among Salmonella spp. isolated from food is most commonly associated with active efflux. Among numerous genetic determinants encoding efflux pumps tetA, tetB, tetC, tetD, tetE and tetG are reported predominatingly. One of the most common mechanisms of resistance against chloramphenicol is its inactivation by chloramphenicol acetyltrasferases (CATs), but resistance to this compound can be also mediated by chloramphenicol efflux pumps encoded by the genes cmlA and floR. It is important to monitor resistance of Salmonella isolated from food, because the globalization of trade, leading to the long-distance movement of goods, animals and food products, encourages the spread of resistant pathogens around the world.
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