Wyniki wyszukiwania

1

Sodium chloride salt stress induced changes in protein content and protease activity in callus culture of pearl millet [Pennisetum glaucum L.R.Br.]

100%

Misra M., Das N., Misra A. N.

Acta Physiologiae Plantarum

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1995

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tom 17

|

nr 4

2

Flower development and senescence in Ranunculus asiaticus L.

84%

Shahri W., Tahir I.

Journal of Fruit and Ornamental Plant Research

|

2011

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tom 19

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nr 2

Flower development of Ranunculus asiaticus L. growing in the University Botanic Garden was divided into six stages (I - VI): tight bud stage (I), loose bud stage (II), half open stage (III), open flower stage (IV), partially senescent stage (V) and senescent stage (VI). The average life span of an individual flower after it is fully open is about 5 days. Membrane permeability of sepal tissues estimated as electrical conductivity of ion leachates (^S), increased as the development proceeded through various stages. The content of sugars in the petal tissues increased during the flower opening period and then declined during senescence. The soluble proteins registered a consistent decrease with the simultaneous increase in specific protease activity and a-amino acid content during different stages of flower development and senescence. The content of total phenols registered an initial increase as the flowers opened, and then declined during senescence.

3

Reduction of milk allergenicity using new synthesized Cu(II) complex as artificial protease

84%

Divsalar A., Saboury A. A., Mansouri-Torshizi H.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

|

nr Suppl.1

4

Regulation of bacterial protease activity

84%

Wladyka B., Pustelny K.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 2

212-229

Proteases, also referred to as peptidases, are the enzymes that catalyse the hydrolysis of peptide bonds in polipeptides. A variety of biological functions and processes depend on their activity. Regardless of the organism’s complexity, peptidases are essential at every stage of life of every individual cell, since all protein molecules produced must be proteolytically processed and eventually recycled. Protease inhibitors play a crucial role in the required strict and multilevel control of the activity of proteases involved in processes conditioning both the physiological and pathophysiological functioning of an organism, as well as in host-pathogen interactions. This review describes the regulation of activity of bacterial proteases produced by dangerous human pathogens, focusing on the Staphylococcus genus.

5

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Changes in enzymatic activity of fish and slaughter animals meat after high pressure treatment at subzero temperatures

84%

Malinowska-Panczyk E., Kolodziejska I.

Polish Journal of Food and Nutrition Sciences

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2018

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tom 68

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nr 2

6

Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions

84%

Dziekonska-Rynko J., Rokicki J., Jablonowski Z.

Wiadomości Parazytologiczne

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2002

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tom 48

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nr 2

The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.

7

The total protein content, protein fractions and proteases activities of drone prepupae of Apis mellifera due to varrosis

84%

Zoltowska K., Lipinski Z., Dmitryjuk M.

Wiadomości Parazytologiczne

|

2005

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tom 51

|

nr 1

The proteins level and activities of acid and alkaline proteases in whole body extracts of drone prepupae of Apis mellifera naturaly infested with Varroa destructor were studied. The infested and a non-infested group did not differ significantly in their total protein content. However, some differences in protein profiles were found. A lack of three protein fractions of moderate and lower molecular weight in infested prepupae was noted. Moreover, some differences in the quantity of protein in most of the fractions were observed. The activity of acid proteases from infested prepupae was lower (p < 0.05) compared with the activity of these proteases from the non-infested one group. The infested drone had higher activity of alkaline proteases than non-infested but this difference was not statisticaly significant.

8

Purification and characterization of an extracellular protease from Bacillus subtilis EAG-2 strain isolated from ornamental plant nursery

67%

Ghafoor A., Hasnain S.

Polish Journal of Microbiology

|

2010

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tom 59

|

nr 2

107-112

Bacillus subtilis EAG-2 strain isolated from an ornamental plant nursery produced a highly active extracellular protease. It was purified to apparent homogeneity by successive purification steps. The SDS-gel of purified protease revealed a single band of 27 KDa on 10% polyacrylamide gel. Proteolytic activity was confirmed by using two different zymographic methods. Interestingly, the enzyme showed two clear activity bands in both cases. The optimum proteolysis for this protease was observed at pH 8.5 and 65°C. The enzyme was highly stable up to 80% after 30-50°C for 60 minutes. It also remained stable at pH 6.5-9.0 after 4 hours of incubation at 37°C. Its activity was reduced to 16% and 25% by PMSF and APMSF which indicates its relation to serine proteases. An increase in activity was noticed in the presence of Ca⁺², Zn⁺² and Ba⁺². On the other hand, it worked effectively with different natural substrates. Hence EAG-2 protease might be a useful contribution to the enzyme industry in Pakistan based upon its distinctive properties.

9

Biochemical characterization of podzolic soil under willow culture after three years from its amendment with sewage sludge

67%

Joniec J., Furczak J.

Polish Journal of Soil Science

|

2007

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tom 40

|

nr 2

207-215

The study was conducted on a podzolic soil (originating from a field experiment), fertilized with various doses of municipal-industrial sewage sludge, i.e. 30 (1%), 75 (2.5%), 150 (5%), 300 (10%) and 600 Mg ha⁻¹ (20%), and then planted with willow (Salix viminalisL.). In the third year from setting up the experiment, in two layers of the soil (0-20 and 20-40 cm) determinations were made on the effect of the applied sludge on the respiratory activity, cellulose mineralization rate, intensification of ammonification and nitrification, and on dehydrogenases and protease activity. The results obtained showed a positive effect of the sludge on almost all of the analysed biochemical parameters, both in the surface and in the deeper layers of the soil. The effect of the sludge in the Ap horizon was more pronounced and generally increased with increasing dosage. Only the process of ammonification was subject to inhibition that was stronger in the surface horizon of the soil.

10

Salinity induced changes in alpha-amylase and protease activities and associated metabolism in cotton varieties during germination and early seedling growth stages

67%

Ashraf M. Y., Afaf R., Qureshi M. S., Sarwar G., Naqvi M. H.

Acta Physiologiae Plantarum

|

2002

|

tom 24

|

nr 1

11

Suppression of Helicobacter pylori protease activity towards growth factors by sulglycotide

67%

Piotrowski J., Slomiany A., Slomiany B. L.

Journal of Physiology and Pharmacology

|

1997

|

tom 48

|

nr 3

12

Distribution and activity of proteolytic bacteria in the surface and subsurface water layers in the region of the Gdansk Deep

51%

Mudryk Z., Donderski W.

Oceanological Studies

|

1996

|

tom 25

|

nr 4

Ograniczanie wyników

Sodium chloride salt stress induced changes in protein content and protease activity in callus culture of pearl millet [Pennisetum glaucum L.R.Br.]

Flower development and senescence in Ranunculus asiaticus L.

Reduction of milk allergenicity using new synthesized Cu(II) complex as artificial protease

Regulation of bacterial protease activity

Changes in enzymatic activity of fish and slaughter animals meat after high pressure treatment at subzero temperatures

Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions

The total protein content, protein fractions and proteases activities of drone prepupae of Apis mellifera due to varrosis

Purification and characterization of an extracellular protease from Bacillus subtilis EAG-2 strain isolated from ornamental plant nursery

Biochemical characterization of podzolic soil under willow culture after three years from its amendment with sewage sludge

Salinity induced changes in alpha-amylase and protease activities and associated metabolism in cotton varieties during germination and early seedling growth stages

Suppression of Helicobacter pylori protease activity towards growth factors by sulglycotide

Distribution and activity of proteolytic bacteria in the surface and subsurface water layers in the region of the Gdansk Deep