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Viral genome DNA from four different multiple-enveloped nuclear polyhedrosis virus isolates, obtained from naturally infected larvae of satin moth (Stilpnotia salicis), a pest of poplar tree (Populus) was analysed. Larvae were collected over a period of 11 years, from 1978 to 1989. The genomic DNA restriction patterns pointed to heterogeneity of these wild-type viruses. The differences observed in isolates of several years revealed limited restriction fragment length polymorphism and showed that these viruses contained distinct, but closely related genotypes. The genome size of SsMNPV was established as 128-134 kb, based on /fmdIII and Sacl restriction analysis.
Until now, there are considerable differences in opinions of both wide public and experts as far as the application of sewage sludge is concerned, especially due to fears concerning their potential contamination with various noxious substances, heavy metals and phytopathogenous germs. Sludge is usually a mixture of mineral and organic sediments, which are trapped during the process of municipal wastewater treatment and contains, among others, also zinc and copper. Both these elements play an important role in growth and development of plants. In our experiments, soil analyses performed 2 to 4 months after application of one or two doses of sludge revealed that the contents of Cu and Zn in soil increased on average by 5.2 - 24.9% as compared to control. However, after one year, their levels decreased to approximately one half and were much lower that the maximum content accepted in light soils in the Czech Republic (i.e. 50 mg·kg⁻¹ and 100 mg·kg⁻¹ for Zn and Cu, respectively). In subsequent years, increasing sludge doses showed a markedly positive effect on the height of poplars (by as much as 63.0 - 106.15%). This means that stabilized and hygienically sound sewage sludge can be used without any problems as a fertilizer for plantations of short rotation energy woody species.
Concentrations of total nonstructural carbohydrates (TNC), soluble carbohydrates, starch, sucrose, glucose, fructose, raffinose, galactose, stachyose, mannitol and specific activities of soluble acid (AI) and neutral (NI) invertases, sucrose synthase (SuSy), hexokinase (HK), fructokinase (FK), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glucose 6-phosphate dehydrogenase (G6PDH) were analyzed in fine roots of Populus deltoides Bartr. ex Marsh growing at a polluted site (near copper smelters) and a control site (free from heavy pollution). Also chemical properties of the soil from both sites were assessed. In comparison with the control, fine roots from the polluted site contained greater concentrations of TNC, soluble sugars, starch and sucrose but less hexoses, so they had higher values of sucrolysis index (sucrose/hexoses). The activity of AI, NI and SuSy declined insignificantly, while specific activities of HK, FK, GAPDH and G6PDH were significantly inhibited. The results suggest that a long-term heavy metal stress leads to an accumulation of carbohydrates and altering activities of glycolysis and the oxidative pentose phosphate pathway in fine roots.
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