Wyniki wyszukiwania - AGRO

1

Can spin labels become effective tools in platelet membrane studies?

100%

Gendek E. G.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

Activity of platelet alpha-6-fucosyltransferase is inversely related to blood platelet concentration

100%

Koscielak J., Antoniewicz-Papis J., Zdebska E., Maj S., Leszko B.

Acta Biochimica Polonica

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1995

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tom 42

|

nr 1

The activity of serum a-6-fucosyl transferase, a platelet derived enzyme, determined in sera of 22 normal individuals and 86 patients with various disorders was positively correlated with platelet counts. When the enzyme activity in 1 jil serum was calculated per 1000 of platelets in blood (coefficient F/P) an inverse correlation became evident in that F/P was proportionally the higher the lower was platelet count in blood. The F/P values were in a good agreement with the results of direct assays of enzyme activities in isolated platelets. Neither granulocytes, lymphocytes nor red cells significantly contributed to serum enzyme activity though granulocytes enhanced the thrombin-induced enzyme release from platelets. In platelets separated by centri- fugation in density gradients the enzyme was shown to be present in platelets of intermediate and high density but missing from the light ones. It is suggested that a-6-fucosyltransferase of platelets may be a marker of the ploidy level of megakaryocytes.

3

The structure of the resting and activated platelet cytoskeleton

88%

Hartwig J. H., Barkalow K., Azim A., Bokoch G., Ohta Y., Nakamura S., Stossel T., Tolias K., Carpenter C.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 2

4

Evidence for existing the Na - H exchanger in porcine blood platelets

88%

Tomasiak M.

Applied Biology Communications. Lublin Scientific Center

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1992

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tom 02

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nr 1

5

Inhibitory effect of quercetin on glycolysis in blood platelets

88%

Tomasiak M.

Applied Biology Communications. Lublin Scientific Center

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1992

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tom 02

|

nr 1

6

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Endotoxin tolerance phenomenon in horses. Observation of changes in the platelet count and fibrinogen concentration

88%

Dabrowska J., Wisniewski E., Krumrych W.

Bulletin of the Veterinary Institute in Puławy

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1999

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tom 43

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nr 2

The subjects were 8 clinically healthy Polish Primitive Horses at 2-9 years of age. The horses were given E.coli LPS four times, in a dose of 0.1 μg/kg.b.w.; 3 times at 24 hour intervals (LPS-1 , LPS-2, LPS-3 ), and one week after the last infusion (LPS-4). The result analysis indicated that the applied endotoxin dose caused development of tolerance mechanisms related to the platelet count. This phenomenon did not concern changes in fibrinogen concentration. The relatively high sensitivity of horses to LPS may be connected with short duration of endotoxin tolerance in this species.

7

The proteome analysis of rat platelet with nano-liquid chromatography-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique

75%

Gromotowicz-Poplawska A., Kasprzyk J., Marcinczyk N., Stepien E., Piekoszewski W., Chabielska E.

Journal of Physiology and Pharmacology

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2018

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tom 69

|

nr 6

8

Platelets and the clinical course of Crohn's disease

75%

Padysz M., Stec-Michalska K., Banasik J., Gasiorowska A.

Health Problems of Civilization

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2018

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tom 12

|

nr 4

Background. Crohn’s disease (CD) is a chronic, autoimmune, inflammatory bowel disease (IBD) characterised by periods of exacerbations and remissions. Autoimmune disorders caused by undetermined factors lead to inflammation in the intestinal mucosa. Presently, there is a growing interest in the role of platelets in the assessment of inflammatory lesions in CD. Accordingly, the aim of this study was to answer the question of whether routinely measured platelet indices: concentration of platelets (PLT), the mean platelet volume (MPV), plateletcrit (PCT) could become biomarkers for monitoring the course of CD. Material and methods. In the study programme, there were enrolled 100 patients with a diagnosed CD with a different clinical course, disease location and heterogeneous therapy. In all patients, there were collected blood and stool samples for the assessment of CRP, blood count and fecal calprotectin evaluation. The clinical state of each patient was classified using the Harvey-Bradshaw index. Results. The study showed a positive, statistically significant correlation between fecal calprotectin, CRP, WBC, the Harvey-Bradshaw index and the number of platelets and PCT. Furthermore, the analysis showed a statistically significant negative correlation between MPV and the number of WBC, CRP and fecal calprotectin. Conclusions. Our study showed that platelet indices are a valuable, non-invasive and widely accessible method to assess mucosal healing and the clinical status of the patient.

9

Effect of bed rest on the adenine nucleotides concentration in human blood platelets

75%

Buczynski A., Kedziora J., Wachowicz B., Zolynski K.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 4

The effect of immobilization in bed on metabolism and function of human blood platelet was studied. Blood platelets taken from patients with bone fractures after long term bed rest (14 days and 28 days) demonstrated significantly reduced concentration of total adenine nucleotides (after 28 days reduction about 30%). This decrease of total platelet adenine nucleotides af er immobilization in bed is probably caused by s imulation of platelet secretory process. Thrombin which released from confrol platele s 58.2%±1.5% of total adenine nucleotides liberated decreased amounfs (only 23.1 %±3.3% of total) of nucleotides from patient platelets isolated after 28 days of immobilization in bed. Loss of nucleotides from platelets was accompanied by sligh ly increased extent of platelet aggregation. It is concluded that during bed rest the reactivity of blood platelets (aggregation and release reaction) is stimulated.

10

Biological significance of chemokine receptor expression by normal human megakaryoblasts

75%

Majka M., Ratajczak J., Baj-Krzyworzeka M., Kijowski J., Reca R., Machalinski B., Kubiczek K., Ratajczak M. Z.

Folia Histochemica et Cytobiologica

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2001

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tom 39

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nr 3

11

Antiplatelet action of losartan involves TXA2 receptor antagonism but not TXA2 synthase inhibition

75%

Chlopicki S., Koda M., Chabielska E., Buczko W., Gryglewski R. J.

Journal of Physiology and Pharmacology

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2000

|

tom 51

|

nr 4,1

12

Effect of rise in intracellular Na and-or cytosolic alkalinization on the aggregation of porcine blood platelets

75%

Tomasiak M.

Applied Biology Communications. Lublin Scientific Center

|

1992

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tom 02

|

nr 6-7

13

Protein kinase c-mediated activation of Na - H exchanger in porcine blood platelets

75%

Tomasiak M.

Applied Biology Communications. Lublin Scientific Center

|

1992

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tom 02

|

nr 3

14

The role of Naplus-Hplus exchanger in serotonin secretion from porcine blood platelets

75%

Tomasiak M., Ciborowski M., Stelmach H.

Acta Biochimica Polonica

|

2005

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tom 52

|

nr 4

This study was undertaken to evaluate whether a link exists between the activation of protein kinase C (PKC), operation of Na+/H+ exchanger (NHE), cell swelling and serotonin (5-HT) secretion in porcine platelets. Activation of platelets by thrombin or phorbol 12-myristate 13-acetate (PMA), a PKC activator, initiated a rapid rise in the activity of Na+/H+ exchanger and secretion of 5-HT. Both thrombin- and PMA-evoked activation of Na+/H+ exchanger was less pronounced in the presence of ethyl-isopropyl-amiloride (EIPA), an NHE inhibitor, and by GF 109203X, a PKC inhibitor. Monensin (simulating the action of NHE) caused a dose-dependent release of 5-HT that was not abolished by GF 109203X or EGTA. Lack of Na+ in the suspending medium reduced thrombin-, PMA-, and monensin-evoked 5-HT secretion. GF 109203X nearly completely inhibited 5-HT release induced by PMA-, partly that induced by thrombin, and had no effect on 5-HT release induced by monensin. EIPA partly inhibited 5-HT release induced by thrombin and nearly totally that evoked by PMA. Electronic cell sizing measurements showed an increase in mean platelet volume upon treatment of cells with monensin, PMA or thrombin. The PMA- and thrombin-evoked rise in mean platelet volume was strongly reduced in the presence of EIPA. As judged by optical swelling assay monensin and PMA produced a rapid rise in platelet volume. The swelling elicited by PMA was inhibited by EIPA and its kinetics was similar to that observed in the presence of monensin. Hypoosmotically evoked platelet swelling did not affect platelet aggregation but significantly potentiated thrombin-evoked release of 5-HT and ATP. Taken together, these results show that in porcine platelets PKC may promote 5-HT secretion through the activation of NHE. It is hypothesized that enhanced Na+/H+ antiport may result in a rise in cell membrane tension (due to cell swelling) which in turn facilitates fusion of secretory granules with the plasma membrane leading to 5-HT secretion.

15

Does membrane fluidity depend on Na - K -ATPase activity?

75%

Walkowiak B., Koziolkiewicz W., Borkowska E., Cierniewski C. S.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

16

Human diadenosine triphosphate hydrolase: preliminary characterisation and comparison with the Fhit protein, a human tumour suppressor

75%

Asensio A. C., Rodriquez-Ferrer C. R., Oaknin S., Rotllan P.

Acta Biochimica Polonica

|

2000

|

tom 47

|

nr 2

Human platelets diadenosine triphosphatase was characterised and compared with the Fhit protein, a human tumour suppressor with diadenosine triphosphatase activity. Both enzymes exhibit similar Km, are similarly activated by Mg2+, Ca2+ and Mn2+, and inhibited by Zn2+ and suramin. However, they are differentially inhibited by Fhit antibodies and exhibit differences in gel-filtration behaviour.

17

Relationship between intracellular pH and aggregation in porcine blood platelets

75%

Tomasiak M.

Applied Biology Communications. Lublin Scientific Center

|

1992

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tom 02

|

nr 2

18

Involvement of Naplus-Hplus exchanger in desmopressin-induced platelet procoagulant response

75%

Tomasiak M. M., Stelmach H., Bodzenta-Lukaszyk A., Tomasiak M.

Acta Biochimica Polonica

|

2004

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tom 51

|

nr 3

Desmopressin (DDAVP) action on platelets is associated with the development of procoagulant response but the underlying mechanism of this phenomenon is not known. We investigated whether this effect of DDAVP might be due to activation of plasma membrane Na+ /H+ exchanger. The DDAVP-induced platelet procoagulant response, measured as phospholipid-dependent thrombin generation, was dose dependent and significantly weaker than that produced by collagen or monensin (mimics Na+ /H+ antiport). Both the DDAVP- and collagen-produced procoagulant responses were less pronounced in the presence of EIPA, an Na+/H+ exchanger inhibitor. Flow cytometry studies revealed that in vitro treatment of platelets with DDAVP or collagen was associated with the appearance of both degranulated (and fragmented) and swollen cells. The DDAVP-evoked rise in size and granularity heterogeneity was similar to that produced by collagen or monensin and was not observed in the presence of EIPA. Using flow cytometry and annexin V-FITC as a probe for phosphatidylserine (PS) we demonstrated increased and uniform binding of this marker to all subsets of DDAVP-treated platelet population. The DDAVP-evoked PS expression was dose dependent, strongly reduced by EIPA and weaker than that caused by monensin or collagen. As judged by optical swelling assay, DDAVP in a dose dependent manner produced a rise in platelet volume. The swelling was inhibited by EIPA and its kinetics was similar to that observed in the presence of monensin. Electronic cell-sizing measurements showed an increase in mean platelet volume and a decrease in platelet count and platelet crit upon treatment with DDAVP. DDAVP elicited a slow (much slower than collagen) alkalinization of platelet cytosol. Altogether the data indicate an involvement of Na+/H+ exchanger in the generation of procoagulant activity in DDAVP-treated platelets.

19

Regulation of transmembrane signaling by the membrane skeleton in platelets

75%

Fox J. E. B.

Cellular and Molecular Biology Letters

|

1996

|

tom 01

|

nr 2

The cytoskeleton is involved in regulating motile events and properties of the plasma membrane. However, it is now clear that the cytoskeleton can bind signaling molecules, recruiting them adjacent to their substrates, and/or inducing their activation. In platelets, the membrane is lined by a skeleton which in turn associates with membrane glycoproteins. Signaling molecules associate with the membrane skeleton in unstimulated platelets and preliminary evidence suggests that components of the membrane skeleton may become phosphorylated on tyrosine residues when platelets are activated. As adhesion receptors bind their ligands, the membrane skeleton becomes more tightly associated with the underlying actin filaments and additional signaling molecules are recruited to the integrin- cytoskeletal complexes. This article describes the evidence for association of signaling molecules with the platelet cytoskeleton and discusses the potential significance of such interactions.

20

Blood platelet secretory activity in the course of giardiosis

75%

Matowicka-Karna J., Panasiuk A.

Wiadomości Parazytologiczne

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1998

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tom 44

|

nr 3

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