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Micropropagation of Calycanthus fertilis

100%

Kulpa D., Kubus M.

Dendrobiology

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2010

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tom 64

Calycanthus fertilis Walt. is a shrub belonging to the family Calycanthaceae, has great potential as ornamental. In the literature there are no reports on methodpropagation of this shrub in in vitro cultures. Therefore, the aim of this study was a development of the method micropropagation of Calycanthus fertilis Walter. Shoot explants of the size 1 cm, with an apex or node with lateral meristems were placed on the media with mineral composition according to MS and WPM supplemented with BAP (from 0.5 to 2.0 mg·dm–3) and TDZ (from 0.1 to 0.5 mg·dm–3). BAP turnedout to significantly increase initiation frequency whereas TDZ inhibitedthe formation of adventitious shoots andcausedexplant death. The highest percentage of initiated explants were found in shoot fragments placed on WPM medium supplemented with 1.0 mg·dm–3 BAP. Primary explants which initiatedgrowth were transferredon the proliferation media containing WPM macroand microelement, with the addition of different cytokinin: BAP (from 0.5 to 2.5 mg·dm–3), KIN (1.0 to 5.0 mg·dm–3) or TDZ (from 0.1 to 0.5 mg·dm–3). Calycanthus multiplication in vitro shouldbe conductedon WPM media with 1.0 mg·dm–3 BAP. Proliferatedshoots were placedon the WPM rooting medium supplemented with auxins: IBA, IAA or NAA at the concentration from 0.1 to 2.0 mg·dm–3. Maximum rooting was obtained on WPM medium supplemented with 0.5 mg·dm–3 IBA. To sum up, it shouldbe statedthat an efficient method of micropropagation of Calycanthus fertilis Walt. has been developed.

2

Effect of epibrassinolide and gibberellin on the stem growth in tulips

86%

Wegrzynowicz-Lesiak E.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2010

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tom 545

The interaction of epibrassinolide (epiBL) with gibberellins (GA3 and GA4+7) in tulip stem growth were studied. When stem length was about 10.0 cm, excision of all leaves and flower bud almost fully inhibited the stem growth in tulips. Epibrassinolide (epiBL) applied at a concentration of 100 nM or 300 nM did not induce tulip stem growth. The application of GA3 or GA4+7 at the concentration of 250 mg·dm-3 alone at all the internodes, after excision of all leaves and flower bud, induced slightly the increase length of all internodes. GA4+7 stimulated stem growth more than GA3. The application of gibberellins in the mixture with epiBL stimulated the growth of tulip shoot much more than gibberellins alone.

3

Proliferation and differentiation of in vitro isolated and cultured endosperm of selected species of Angiospermae

86%

Bras M., Nowakowski P., Rzenno K., Popielarska-Konieczna M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

4

Effect of nutrient media and phytohormones on in vitro establishment of Bambusa balcooa Roxb.

86%

Khan H. R., Burla S., Siri N., Lavanya P.

International Letters of Natural Sciences

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2014

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tom 12

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nr 1

Bambusa balcooa.Roxb. is one of the most commercially important bamboo species. Some varieties of bamboo are being grown in the company’s green house. However this species is very rare and it was not cultured until now due to lack of demand and maintenance problems. In this work B. balcooa ex-plants have been established and propagated by the axillary shoot bud proliferation method. Earlier several works have been done on this plant and a protocol has been. This work emphasizes on effects of phytohormones at different concentrations and combinations upon the novel propagules that develop from the bamboo explants on placing them in multiplication media. The propagules placed in media containing cytokinins BAP (2 mg/L) and Kinetin (1 mg/L) exhibited best results of linear as well as radial growth. The other propagules in media with combinations of BAP, Kinetin and NAA were found to be dried out and did not depict noticeable growth. Comparatively next best growth pattern to the former was observed in control media that lacked phytohormones. This experiment was helpful in estimating the quantity and effect of particular plant growth regulating hormones on the Bambusa balcooa.Roxb.

5

Productivity of hulless oats under the effect of microbiological preparation and a plant growth regulator

86%

Karpenko V., Marchenko K.

Acta Scientiarum Polonorum. Agricultura

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2021

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tom 20

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nr 3

6

Daminozide - lack of the genotoxic activity in the short-term bacterial test

86%

Rahden-Staron I., Szumilo M., Ziemkiewicz P., Szymczyk-Wasiluk T.

Roczniki Państwowego Zakładu Higieny

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1997

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tom 48

|

nr 2

In the recent paper genotoxic effects of daminozide and its metabolites were tested. Evaluation of the mutagenic effect of daminozide was by: (i) the Salmonella/mammalian microsome Ames test with S.typhimurium TA97, TA98, TA100 and TA102. (ii) E. coli PQ37 strain to reveal an induction of the SOS response, (iii) S. typhimurium TA1538 (uvrB) and TA1978 (uvr+) to detect the chemicals bound covalently to DNA (repair test). Daminozide was not mutagenic in any of the S.typhimurium strains and did not induce damages in DNA recognized by correndonuclease II, as shown by the repair test. Only metabolites of daminozide induced the SOS system.

7

Anethum graveolens L. in vitro cultures - a potential source of bioactive metabolites, phenolic acids and furanocoumarins

72%

Szopa A., Ekiert H.

Acta Biologica Cracoviensia. Series Botanica

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2015

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tom 57

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nr 2

8

How do rare earth elements (lanthanoids) affect root development and protocorm-like body formation in hybrid Cymbidium?

72%

Teixeira da Silva J. A.

Journal of Horticultural Research

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2014

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tom 22

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nr 1

9

Growth and yield response of two hybrid rice cultivars to ATONIK plant growth regulator in a Tropical environment

72%

Banful B. K., Attivor D.

Environment, Earth and Ecology

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2017

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tom 1

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nr 1

A study was conducted at the Department of Horticulture, KNUST, Kumasi from June to November, 2015 with the objectives to (i) determine the rate of ATONIK plant growth regulator (PGR) suitable for high yield of two varieties of hybrid rice (ii) determine the combined effects of PGR rates and varieties on the growth and yield performance of hybrid rice. A 2 x 5 factorial arrangement in randomized complete block design with three replications was used. The factors were varieties at two levels: Agra Rice and Jasmine 85 and PGR at five levels: ATONIK at 450 ml/ha, ATONIK at 500 ml/ha, ATONIK at 550 ml/ha, ATONIK at 0 ml/ha and GA3 at 60 ml/ha. Comparing the ATONIK rates with the GA3, ATONIK at 450 ml/ha resulted in a 14.3 % increase in the number of rice panicles. Application of ATONIK at 450 ml/ha, 500 ml/ha and 550 ml/ha resulted in 14.4%, 10.7% and 4.4% higher percentage of productive tillers, respectively, than that produced by GA3 at 60 ml/ha. ATONIK at 450 ml/ha application led to a 17.8 % increase in grain yield. For the harvest index, application of ATONIK at 450 ml/ha resulted in the highest harvest index of 45 %, significantly greater than the other PGR treatments. In conclusion, the study clearly demonstrated that ATONIK PGR was superior to GA3 in the vegetative and productive performance of rice. The most suitable rate of ATONIK for increased rice productivity was 450 ml/ha.

10

In vitro flowering of Petunia x atkinsiana D. Don

72%

Kulpa D., Nowak N.

Folia Horticulturae

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2011

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tom 23

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nr 2

11

Quantification of taxol by high-performance thin layer chromatography in Taxus wallichiana callus cultivated in vitro

72%

Mujib A., Tonk D., Gulzar B., Maqsood M., Ali M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2020

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tom 101

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nr 4

12

Nitric oxide trascriptional players during seed dormancy and germination

72%

Albertos P., Mateos I., Fernandez-Arbaizar A., Romero-Puertas M., Lorenzo O.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

13

Control of growth and development of Ceratocystis fimbriata Ell. et Halst. by plant growth regulators. I. Auxins

72%

Stopinska J., Michniewicz M.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 10-12

14

Effect of ethrel on the starch sugar changes of off-season fruits of mango (Mangifera indica l. var. Neelum) during ripening

72%

Venkatesan T., Tamilmani C.

International Letters of Natural Sciences

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2014

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tom 02

The present investigation is aimed at studying the effect of ethrel on the ripening of off-season fruits of Mangifera indica L. var. Neelum. The control fruits were kept in the laboratory naturally while the experimental fruits were treated with different concentrations of ethrel (100, 200 and 300 ppm). In control fruits, partial ripening led to incomplete metabolic changes, which did not alter the presence of sourness in the fruits. Hence, they were not fit to be eaten. On the other hand, the fruits treated with different concentrations (100, 200 and 300 ppm) of ethrel ripened on 13th day, 11th day and 9th day respectively after treatment. The colour changed from green greenish to yellow and the fruits were palatable in nature. The starch decreased during ripening, both in the treated and control fruits. On the other hand, the sugar, alpha-amylase, beta-amylase, activities increased. Among the different 100, 200 and 300 ppm ethrel treatments, the 200 ppm alone had the optimum effect on the ripening of off-season fruits of Mangifera indica L. var. Neelum.

15

Plant growth regulator induced phytochemical and antioxidant variations in micropropagated and acclimatized Eucomis autumnalis subspecies autumnalis (Asparagaceae)

72%

Masondo N. A., Aremu A. O., Finnie J. F., Van Staden J.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

Eucomis species is a valuable plant with both medicinal and horticultural potential. The current study evaluated the role of plant growth regulator (PGR) on growth, phytochemicals, and antioxidant activity in Eucomis autumnalis subspecies autumnalis. Five cytokinins including topolins and benzyladenine (BA) at 2 µM in combination with varying (0–15 µM) concentrations of naphthalene acetic acid (NAA) were tested. In vitro regenerants were acclimatized in the greenhouse for 4 months. Highest number of shoots (9 shoots/explant) was observed with 15 µM NAA alone or when combined with BA. Acclimatized plants derived from the 15 µM NAA treatment had the highest number of roots, largest leaf area and biggest bulbs. While applied PGRs increased the iridoids and condensed tannins in the in vitro regenerants, total phenolics and flavonoids were higher in the PGR-free treatment. Among the in vitro regenerants, 5 µM NAA and 2 µM BA treatments produced the best antioxidant activity in the DPPH (55 %) and beta-carotene (88 %) test systems, respectively. A remarkable carry-over effect of the PGR was conspicuous in the phytochemical levels and antioxidant activity of the 4-month-old plants. In addition to the optimized micropropagation protocols, the current findings present a promising potential for manipulating the type and concentration of applied PGRs to improve phytochemical production and hence medicinal value in E. autumnalis subspecies autumnalis.

16

Influence of selected agronomic measures on the content of some mineral elements in grain of naked oat (Avena sativa L.)

72%

Witkowicz R., Antonkiewicz J.

Acta Scientiarum Polonorum. Agricultura

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2009

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tom 08

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nr 4

Field experiments were conducted in two localities: Prusy (50°07’ N; 20°04’ E – one experiment) and Wierzbica (50°29’ N; 19°45’ E – two experiments) in 2003. The objective of this study was to analyse the influence of agronomic factors on the grain yield and on the content of macroelements in grain of different forms of naked oat. In Wierzbica the grain yield was determined statistically by the genotype, the phosphorus and potassium fertilization and the application of the plants growth regulator Moddus. In Prusy grain yield was determined only by the genotype (cultivars, strains). A concentration of macroelements in forms of oat was statistically different. In both localities the phosphorus and potassium fertilization and foliar application of urea, in general, had not the statistical influence on the content of the macroelements. An exception is the influence of the foliar application of urea on the content of potassium. Plant growth regulator Moddus caused changes in the content of macroelements. These changes were not always statistically significant, but always increased the concentration of macroelements. The second plant growth regulator Promalin did not cause changes in the concentration of macroelements.

17

Histological analysis of organogenesis and somatic embryogenesis during shoot formation in sugar beet (Beta vulgaris L.) via gynogenesis

72%

Tomaszewska-Sowa M., Figas A., Gatz A.

Polish Journal of Natural Sciences

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2017

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tom 32

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nr 4

The aim of the present research were histological analysis of regenerating structures through in vitro gynogenesis from unfertilized ovules of sugar beet (Beta vulgaris L.). The process of shoot regeneration using a novel two stage method combines the preculture in liquid medium with the culture on solid medium. The highest number of explants that formed shoots (60%) was observed on medium supplemented with BAP, sucrose and gerlite, and as regards carbohydrates used in the medium most of explants forming shoots (42%) and the largest total number of shoots (110) was observed for glucose. To accurately determine the course of shoot formation, histological analyses were performed. Careful histologic evaluation of regenerating structures revealed the presence of numerous meristematic centres. In some meristems formation of specialized tissues and organs was observed, including epidermis, apical meristem, leaf primordia and tracheal elements. The analyses showed that the regeneration of the new structures from sugar beet ovules occurred both through organogenesis as well as somatic embryogenesis since the presence of somatic embryos in the globular stage or torpedo stage were observed.

18

A non caloric sweetener Stevia rebaudiana Bertoni - tissue cultured plantlets for organic farming and home gardening

72%

Tomaszewska-Sowa M., Figas A., Sawilska A. K.

Infrastruktura i Ekologia Terenów Wiejskich

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2015

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nr IV/3

19

Effects of thidiazuron on in vitro shoot regeneration of Morus alba

72%

Rezaei-Zafarghandi M. S., Rahmati-Joneidabad M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2020

|

tom 101

|

nr 1

20

Control of growth and development of Ceratocystis fimbriata Ell. et Halst. by plant growth regulators. III. Cytokinins

72%

Stopinska J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1991

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tom 39

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nr 3

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