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We briefly review the limited application of marker assisted selection in past wheat breeding programmes, and contrast the current situation, where increasingly it has become feasible to tag almost any gene with a microsatellite assay. Although this capability is having an impact on the conduct of large breeding programmes, a much more profound change in breeding strategy will become possible when SNP technology has matured sufficiently so that the throughput of molecular marker-based genotyping will be able to keep pace with the numbers of plants that breeders can handle in the field. We discuss the considerations that will need to be addressed in the generation of a new breeding paradigm to take advantage of the genomics revolution.
Molecular markers have become a useful tool simplifying and speeding up breeding work. They are also helpful in the genetic analysis of complex agronomic traits. The investigations concern the use of already available SCAR markers for molecular analysis of breeding materials. The markers are the apple powdery mildew resistance gene Pl2, originating from Malus zumi, and the apple scab resistance gene Vf, from M. floribunda 821. The clone U 211 was found to be highly resistant to mildew under field conditions and transmitted a high level of resistance to the majority of its progeny. The presence of the Vf gene conferring resistance to scab was confirmed by molecular analysis. As the molecular markers for the Pl2 gene are not present in the DNA of U 211, it is probable that the clone U 211 is a new source of mildew resistance.
Great advances have been made in recent years in marker detection systems and in the techniques used to identify markers linked to useful traits. While RFLP markers have been the basis for most work in crop plants, useful markers have been generated using RAPD and AFLP methods. More recently, microsatellite or simple sequence repeat (SSR) markers have been developed for major crop plants and this marker system is predicted to lead to even more rapid advances in both marker development and implementation in breeding programs. Identification of markers linked to useful traits has been based on complete linkage maps and bulked segregant analysis. However, alternative methods, such as the construction of partial maps and combination of pedigree and marker information, have also proved useful in identifying marker/trait associations. The value of markers in analysing the inheritance of traits in crop plants and understanding genome structure and organization is now well established. The different properties of markers systems and their applications in genome analysis and molecular breeding of cereals species are discussed.
Honeyberry’s popularity has been increasing because of its valuable traits: nutritional value of fruits (a high amount of ascorbic acid, potassium and polyphenolic compounds), early ripening (14 days before strawberry), exceptional hardiness and easy cultivation. However, there is still little known about the pollination biology of this species, which is essential for practical breeding purposes. In this study we evaluated pollen fertility in five cultivars and three breeding clones of this species. The impact of the manner of pollination on the fruit set and forming seeds has also been studied. The highest percentage of viable pollen was found in cultivars ‘Duet’ and ‘Wojtek’ (96%), whereas the pollen of ‘Chelyabinka’ cultivar had the lowest fertility (90%). The percentage of blue honeysuckle fruits obtained from open-pollinated flowers was high (on average 94.7%) compared to isolated flowers (on average 8.6%). The effect of cross-pollination with cv. ‘Duet’ indicates that the percentage of the fruit set, fruit weight and mass of produced seeds was higher when this cultivar was applied as maternal than paternal form. The number of seeds affected significantly the weight of the fruit in the case when cv. ’Duet’ was used as a paternal (correlation coefficient r = 0.45) or maternal form (r = 0.66), while for other methods of pollination this effect was insignificant. These results may be used to the most efficient establishment of plantations as well as for the realization of the breeding program of this valuable species.
This paper presents some partial results of a study on the financial consequences of lupine breeding in Poland, as well as market conditions for seed trade. Analysis also covers the impact of breeding fees on the financial performance of this business. The research procedure was based on a case study of two domestic plant-breeding companies. The study includs the analysis of funding sources used for lupine seed breeding and the potential financial outcomes of this activity. The plant-breeding companies, covered by this study, are owned by the State Treasury and are organized so that the breeding department is part of a larger agricultural undertaking engaged in multiple production activities. Therefore, accounting data of these undertakings was used in order to determine the economic performance of the breeding department. Also, direct interviews were conducted with breeding department employees and top-level managers. A simulation calculation was also performed under the assumption that the breeding department is an independent undertaking which settles its accounts with the holding on a commercial basis. Therefore, the breeding department was assumed to sell intellectual property consisting of seed fraction “E.” The plant-breeding company is also a sort of seed company which sells the marketable seed fraction K. In summary, lupine breeding is concluded to be a highly profitable business, provided that the plant-breeding departments fully settle their accounts internally and that the breeding fees from seed companies are fully collectable. The development and production potential identified in this study suggests these plants could be used to increase national self-sufficiency in vegetable protein.
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