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The aim of our work was to investigate the antioxidant activity of Rheum palmatum extracts. Antiradical activity against DPPH and ABTS radicals, reducing power FRAP and total phenolic contents, were investigated in one-, two- and three-year-old roots of rhubarb fertilized with nitrogen at the rates of 50, 100 and 200 kg N/ha. It was proved that nitrogen dose as well as the age of plantation did significantly influence antioxidant activity and total phenolic of root extracts. The highest values were determined in one-year-old plants, antioxidant activity ranged the level of 112–203 µM Trolox/g and total phenolic compounds average content was 22 mg GAE/g FW. Two-year-old roots were characterized by 3–11%, and three-year-old ones by 15–23% lower antioxidant activity and phenolic compounds content. When nitrogen dose increased, polyphenols content, DPPH and FRAP values increased as well, although, ABTS showed a different tendency.
The fruit of black chokeberry (Aronia melanocarpa (Michx.) Elliott) is valuable raw material for food and pharmaceutical industries due to the content of anthocyanins, but also other components. The aim of this study was to estimate the content of dry matter, reducing sugars, vitamin C, anthocyanins and the antioxidant potential in black chokeberry fruits depending on the age of plants and the time of fruit harvest. Material for the study was collected in 2011 and 2012 from 6–7, 11–12 and 16–17-year-old plants. Fruits for analyses were harvested on 10–12 August, 29 August – 1 September, 11–13 September, 26–27 September, 11–14 October, 25–27 October. Fruits from the oldest plants contained 16–18% less anthocyanins and 8% less vitamin C, but 2.7 percentage points more dry matter than the fruits from younger plants. On the 29 August–1 September fruits achieved the maximum content of reducing sugars, vitamin C and anthocyanins, 13.7, 8.2 and 715.5 mg 100 g-1, respectively. The content of reducing sugars and anthocyanins stayed unchanged until the end of October, and the content of vitamin C decreased considerably. Antioxidative potential (DPPH) ranged from 77.0 to 85.2% inhibition and it did not depend on the age of harvest time of fruits. The most valuable chokeberry fruits were obtained from plants up to 12 years of age, harvested between 29 August and 13 September.
The present research was conducted in managed stands of Scots pine (Pinus sylvestris L.) at different age in Poland. All the investigated stands were planted and thinned according to selection thinning. Analysis of spatial structure concerned trees of different categories (DBH classess) depending on the age of stand. Spatial distribution of trees was investigated using two commonly used methods: Clark-Evans R index and Ripley's L(t) function. In stands of age 43 and 65 the effect of the initial spacing was observed and all living trees were distributed regularly at the smallest distances and at larger spatial scale they were spaced randomly. Random type of spatial structure of trees was observed when smaller and larger trees were taken into consideration, separately. In case of stands at the age 90 the effect of the initial planting on the spatial structure of trees was not observed even at the smallest distances independently of the category of trees taken into account. All living trees in these stands were distributed randomly. Only in two stands clumps of trees were observed and one concerned smaller trees and the other - larger trees. Size differentiation index was small for all stands. The lowest values was ascertained in the oldest stands.
Asparagus plants cv. “Epos” were planted in the aeroponic system with recirculation in two cycles. The effect of asparagus plant age and crown size on fern growth were studied. The results show that the age of asparagus plants affected the number of ferns – the number of assimilation shoots increased with age – while the weight of asparagus crowns had an effect on the number and total weight of shoots. An increase in crown weight by about 1000 g caused an increase in the number of shoots by about 6, while the weight of one shoot increased by approx. 144 g. The age of aasparagus plants was also a significant factor for dry weight, % Brix and total carbohydrate contents in roots before the assimilation season as well as glucose, fructose, sucrose, GFS and total carbohydrate contents in roots after the assimilation season.
Four- and five-year-old plants gave a distinctly higher yield of seeds in comparison with six- year-old plants. Seeds originating from younger plants were also characterised by higher indi­vidual weight. Germination ability of seeds was affected both by age of mother plants and by temperature and presence of light during germination process. Higher germination ability and higher weight of seedlings was observed at substrate pH 5.5 and 6.5 in contrary to pH 3.5.
The published results on plant propagation m vitro culture show the importance of maternal plant age in a successful regeneration process. It is known that initiation effectiveness is low when primary explants are taken from old trees. The aim of this study was an attempt to regenerate Syringa vulgaris from an old specimen of Dendrological Garden in in vitro culture. April turned out to be the optimal month for collecting explants. The highest percentage of initiated shoots were obtained on MS medium containing 7.5 mg⋅dm⁻³ BAP and 0.02 mg⋅dm⁻³ NAA. The highest propagation efficiency was received on MS medium with a double amount of MgSO₄ supplemented with 1.0 mg⋅dm⁻³ BAP. Insignificant effects were observed on the media with cytokinin BAP. The plants with the best-developed root system were obtained on MS medium with reduced to ¼ amount of macro- and micronutrients supplemented with 5.0 mg·dm⁻³ IBA.
Celem pracy była ocena możliwości reprodukcyjnych szczodraka krokoszowego Rhaponticum carthamoides (Willd.) Iljin w warunkach klimatycznych Polski, a w szczególności określenie wpływu niektórych czynników rozwojowych (wiek roślin, stopień dojrzałości nasion oraz miejsce ich osadzenia w koszyczku) na masę nasion i ich zdolność kiełkowania. Badano również wpływ stratyfikacji oraz przedsiewnego traktowania nasion kwasem giberelinowym na ich kiełkowanie. Doświadczenia przeprowadzono na Polu Doświadczalnym SGGW w latach 1999-2000, na 2-, 3- i 4-letniej plantacji szczodraka. Plon nasion z 1 rośliny był wyższy u roślin 3-letnich, które tworzyły większą liczbę pędów kwiatostanowych, a w związku z tym więcej koszyczków niż rośliny 2- i 4-letnie. Natomiast masa nasion w 1 koszyczku, jak i masa 1000 nasion, nie zależała od wieku roślin. Istotny wpływ na zdolność kiełkowania nasion miało miejsce ich osadzenia w koszyczku. Wyższą masą jednostkową (masą 1000 nasion) i wyższą zdolnością kiełkowania charakteryzowały się nasiona z zewnętrznej części koszyczka. Masa nasion uzyskanych z 1 koszyczka wynosiła 5-6 g, z czego 70-90% stanowiły nasiona w pełni wykształcone. Masa 1000 nasion, w zależności od wielkości koszyczka i miejsca osadzenia nasion w koszyczku, wahała się w granicach 12-20 g. Zdolność kiełkowania dojrzałych nasion szczodraka, pozyskanych z uprawy w naszych warunkach klimatyczno-glebowych, okazała się dość wysoka (ok. 70-80%). Stratyfikowanie nasion wpłynęło na przyspieszenie pojawiania się wschodów roślin, jednak nie miało wpływu na ostateczną liczbę skiełkowanych nasion. Istotne zwiększenie zdolności kiełkowania nasion uzyskano w wyniku przedsiewnego traktowania ich roztworem GA₃ o stężeniu 500 ppm.
In this experiment a few physiological characters were measured in order to show differences in leaf water potential (Ψ), chlorophyll content (SPAD) and chlorophyll a fluorescence (F0, FM, FV, FV/FM) between leaves differing in age of 3 poplar cultivars inoculated with H. crustuliniforme. In control plants (non-inoculated) differences in water potential and chlorophyll fluorescence parameters between the examined cultivars were slight. Statistically significant differences in water potential and chlorophyll content between non-inoculated and inoculated plants were observed only for young leaves from the upper parts of the plant stem. Similarly, significant differences between treatments in initial fluorescence (F0), maximal fluorescence (FM) and in variable fluorescence (FV) were found in leaves from the middle and upper part of the plant stem. The obtained results indicated that for leaves from the upper, median and lower part of the plant stem significant differences between treatments in maximum quantum yield of PS2 (FV/FM) in leaves from different plant parts in P. petrowskayana were not observed and in P. deltoides cv. Plantierens and P. balsamifera only for leaves from the upper parts of the plant stem. Although those differences between inoculated and non-inoculated plants were relatively small, we may presume, that if they occur through a longer period they may become more distinct causing positive changes in leaves. In conclusion, it is necessary to саrrу out future studies on the mechanism by which the EMC symbiosis influences leaf physiological traits.
Presented analyses were connected with modelling foliage biomass of Scots pine (Pinus sylvestris L.) growing in northern and western Poland. In order to realize the study, a total of 38 one-hectare experimental plots were established in commercial pine monocultures growing on sites optimal for this species at a given geographical location. A total of 114 trees aged 32-114 years were felled and their needle weight and the weight of needles and young, 1-year old twigs were measured. Since social variation in the stand could affect the fit of the model, for each social class of tree position an individual allometric equation was proposed, based on easily measurable biometric traits of trees. Analysis of stepwise backward regression was conducted in order to determine variables in these equations. It turned out that to estimate foliage biomass of predominant and dominant trees, it seems most appropriate to apply diameter at breast height and tree age. In turn, for co-dominant trees, apart from diameter at breast height and tree age, the model needs to include additionally tree height and crown length. Moreover, due to the fact that the dependence between needle weight and the weight of needles and young twigs is practically linear, prediction was conducted for the biomass of foliage with young twigs. From the practical point of view, this makes it possible to precisely determine needle biomass on the basis of the weight of needles with young, 1-year-old shoots.
The aim of undertaken research was recognition the reasons of dying out the common juniper Juniperus communis L. ssp. communis population in the forest nature reserve “Jałowce” (Pomerania). Applied methods of dendrochronological analysis let to determine the age of juniper-stand in the reserve and describe the influence of climatic conditions on its growth. Obtained results testify to Juniperus communis chronology with signature WIE collected from 17 individual sequences which numbered 102 tree ring width and represented a time span 1903 to 2004. Such results described the juniper-stand in the reserve as ageing population with the oldest specimen at age 98 years. However, the last several years were characterised by the lack of strong growth depressions typical for earlier time periods with tree ring width ranged 0.7–1.0 mm (themean annual tree ring width amounted to 0.85 mm), but the visible symptoms of degeneration and dying out of protected common juniper population intensified probably because of its age.
Herba Polonica
|
2009
|
tom 55
|
nr 3
63-68
The studies were conducted in the years 2004–2008. The available Polish and foreign literature does not devote much attention to the problem of growing white horehound. The cropping of white horehound grown from sowing and seedling was assessed in the experiment, depending on the term when the plantation was established, harvest date and age of the plants. Air dry herb was analyzed in the laboratory, where the essential oil content was determined. On the basis of the studies, it was revealed that seed sowing date and herb harvest term affect white horehound plant cropping. Differences were also found in cropping between plants from one-year and two-year plantation
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