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  1. 17 Acta Biochimica Polonica

  2. 5 Folia Histochemica et Cytobiologica

  3. 2 Archivum Immunologiae et Therapiae Experimentalis

  4. 2 Cellular and Molecular Biology Letters

  5. 2 Polish Journal of Microbiology

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  1. 6 Frackowiak D.

  2. 5 Banas T.

  3. 5 Chwilkowska A.

  4. 5 Saczko J.

  5. 5 Wiktorowicz K.

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  1. 1 2020

  2. 2 2018

  3. 1 2013

  4. 1 2012

  5. 2 2011

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1

Incorporation of stilbazolium merocyanines into resting and stimulated mononuclear leukocytes

100%
Frackowiak D., Wiktorowicz K., Cofta J., Niedbalska M., Latosinska M.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 1
Stimulated and resting mononuclear leukocytes were incubated with a stilbazolium merocyanine dye l-(6'-hydroxyhexyl)-4-[(4-oxocyclohexa-2,5-dienylidene)ethylide- ne]-l,4-dihydropyridine and immobilized in isotropic and stretched polyvinyl alcohol film. Polarized absorption, fluorescence and fluorescence excitation spectra were collected and the anisotropy of absorption and emission were calculated. Analysis of the spectra pointed to: i. the occurrence of perturbation of the membrane structure by incubation with the dye, and ii. influence of the blood serum addition, during the process of incubation with.the dye, on the efficiency of incorporation of merocyanine into the cells and the degree of the dye orientation in the membrane. A small fraction of the dye molecules introduced into resting cells was found oriented to a higher degree than a large fraction incorporated into stimulated cells. The incubation time longer than 15 min caused strong changes in the membrane structure both of the resting and stimulated cells.
2

Photodynamic effect of lanthanide derivatives of meso-tetra[N-methyl-4-pyridyl]porphine against Staphylococcus aureus

88%
Jurczak A., Szramka B., Grinholc M., Legendziewicz J., Bielawski K. P.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 3
581-585
Photodynamic therapy (PDT), used for cancer treatment, is also an alternative method for eradication of drug-resistant bacteria. This method utilizes a nontoxic light-activated dye, called a photosensitizer, and visible light to produce reactive oxygen species that lead to bacterial cell death. The purpose of this study was to investigate the bactericidal effect of PDT using lanthanide derivatives of meso-tetra(N-methyl-4-pyridyl)porphine against Staphylococcus aureusstrains. The new photosensitizers appeared to be photodynamically ineffective. No enhancement of antistaphylococcal activity of TMPyP was observed after the conjugation of the porphyrin with lanthanide ions. Additionally, a significant difference in the susceptibility of two bacterial strains to unmodified TMPyP was observed.
3

Studies on the effect of photodynamic therapy on in vitro cultured neoplastic cells

88%
Wolun-Cholewa M., Warchol J. B.
Folia Histochemica et Cytobiologica
|
2001
|
tom 39
|
nr 2
4

Application of photodynamic therapy in the sterilisation of tumour cells transplanted into laboratory animals

75%
Jurczyszyn K., Ziolkowski P., Klytta M., Piasecki T.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 1
123-128
In this study, we tried to estimate the effectiveness of the antimicrobial activity of photodynamic therapy (PDT) without the destruction of tumour cells. Chlorin e6 in concentrations from 0.05 to 1.0 µg/mL and total dose of light 100 J/sq.cm were used. Samples from different tumours were tested for a presence of bacteria. Next, cells from the same tumours were treated with chlorin e6-PDT, chlorin e6, and light alone, or not treated. The survival rate was counted, and the cells were injected back into the animals. PDT caused the eradication of bacteria such as Proteus sp., Streptococcus α-haemolyticus, and others: the specimen became aseptic. After Ce6-PDT treatment and in control groups, tumour growth was observed in all rats 7-10 d after transplantation. The tumours were palpable and macroscopically visible. Our studies showed that PDT kills bacterial flora without the destruction of tumour cells. Thus, tumour cells free of bacteria could be used for other experiments.
5

Orientation and spectral properties of two stilbazolium merocyanine dyes in stretched and unstretched polyvinyl alcohol films

75%
Dudkowiak A., Frackowiak D., Teslak E., Gryczynski Z., Gryczynski I.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 3
Spectral properties (anisotropy coefficients calculated for absorption, emission and fluorescence decay time) of two stilbazolium merocyanine dyes have been determined to evaluate the applicability of these dyes as sensitizers in photodynamic therapy. The dyes were embedded in an anisotropic polymer matrix. Analysis of the emission decay components measured in polarized light provides information on the interactions of the dye molecules with the polymer matrix being a model of an anisotropic biological system. Different values of the emission anisotropies obtained from various polarized components of fluorescence decays have shown that the orientations of the dye molecules influence their interactions with the polymer. This means that differently oriented dye molecules located in biological systems should exhibit different interactions with membranes. The chain length and type of side groups attached as well as the salt form of the dye molecule were shown to influence the dye-polymer interactions and should be taken into account before the application of merocyanine dyes in medicine. These dyes seem to be promising optical sensors with spectral properties, including the calculated anisotropy coefficients, sensitive to the molecular environment, useful to study orientation and interaction with neighbouring molecules in biological membranes.
6

The interactions of phthalocyanines with stimulated and resting human peripheral blood mononuclear cells

75%
Frackowiak D., Waszkowiak A., Manikowski H., Ion R. M., Cofta J., Wiktorowicz K.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 1
The interactions of two metal-free phthalocyanines [(H2Pc) and Solar Pc (with four peripherical groups: SO2N(CH2CH2OH)2)] and of one metal substituted dye (CoPc) with resting and stimulated human peripheral blood mononuclear cells (PBMC) were com­pared. The absorption, fluorescence, photoacoustic and EPR spectra of both resting cells and cells stimulated by phytohaemagglutinin, incubated in dimethyl sulfoxide (DMSO) with very low or 95% water content and with or without dye addition, were measured. The fate of the light absorbed by the samples was investigated. It is known that singlet oxygen pro­duction is crucial for photodynamic action of dyes. Thermal deactivation and lumines­cence emission compete with this process, so investigation of these alternative paths of sensitizer deactivation provides information about photodynamic action. The incorpora­tion of the investigated dyes into cells and the perturbation of the cell structure caused by the dyes, the incubation solvent and the activator were investigated by comparing the spectral properties of PBMC before and after stimulation and incubation. Incubation of the cells for 1 h in a solution of Solar Pc in 99.5% aqueous DMSO, resulted in an efficient dye incorporation which was highly selective. Solar Pc being introduced much more effi­ciently into stimulated cells than into resting cells.
7

Cytosolic superoxide dismutase activity after photodynamic therapy, intracellular distribution of Photofrin II and hypericin, and P-glycoprotein localization in human colon adenocarcinoma

75%
Saczko J., Kulbacka J., Chwilkowska A., Pola A., Lugowski M., Marcinkowska A., Malarska A., Banas T.
Folia Histochemica et Cytobiologica
|
2007
|
tom 45
|
nr 2
8

Preliminary studies of phthalocyanine sensitizers incorporated into human leukemia cells from two cell-lines

75%
Wiktorowicz K., Cofta J., Dudkowiak A., Waszkowiak A., Frackowiak D.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 3
Three phthalocyanine dyes-sensitizers were incorporated into two types of human T leukemia cells from two cell-lines: CCRF and MOLT 4. The efficiency of the dye in­corporation into cells and photochemical properties of stained cells were investi­gated using fluorescence spectroscopy. The dyes exhibited different properties in each of the two cell-lines. Small differences in cell membrane properties have a strong influence on the efficiency of dye incorporation and on the course of photodynamic reaction. It is suggested that, for a given patient, an optimal dye-sensitizer should be established before photodynamic treatment.
9

Effect of 5-aminolevulinic acid on kinetics of protoporphyrin IX production in CHO cells

75%
Wolun-Cholewa M., Warchol W.
Folia Histochemica et Cytobiologica
|
2004
|
tom 42
|
nr 2
10

Tritolylporphyrin dimer as a new potent hydrophobic sensitizer for photodynamic therapy of melanoma

75%
Drzewiecka A., Urbanska K., Matuszak Z., Pineiro M., Arnaut L. G., Habdas J., Ratuszna A., Stochel G.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 1
We report the synthesis, photochemical and photophysical properties and preliminary studies on biological effect of a new tritolylporphyrin dimer (T-D). Absorption and emis­sion properties of T-D suggest its possible use in photodynamic therapy. T-D is capable of singlet oxygen production with 0.8 quantum yield. It also has a high photostability. The photodynamic properties of the dimer were examined following the growth of SKMEL 188 (human melanoma) cells irradiated with red light (cut off < 630 nm). The surviving frac­tion of the cells decreased about 3-fold (us. non-irradiated cells) for an 81 J/cm dose. Our results suggest that tritolylporphyrine dimer T-D may be an interesting hydrophobic sensitizer for photodynamic therapy.
11

5-aminolevulinic acid photodynamic therapy of transplantable colon adenocarcinoma in BALB-c mice

75%
Ziolkowski P., Symonowicz K., Osiecka B. J., Rabczynski J.
Archivum Immunologiae et Therapiae Experimentalis
|
1998
|
tom 46
|
nr 5
12

Apoptosis and necrosis in normal and malignant cells during photodynamic therapy

75%
Marcinkowska A., Malarska A., Saczko J., Chwilkowska A., Banas T.
Folia Histochemica et Cytobiologica. Supplement
|
2001
|
tom 39
|
nr 1
13

Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells

75%
Waszkowiak A., Frackowiak D., Wiktorowicz K., Miyake J.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 3
Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solu­tions of various phthalocyanines. In order to diminish the influence of atmospheric ox­ygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong ab­sorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. This suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. The ab­sorption spectra of samples were also measured. The spectra of various phthalo- cyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time peri­ods enabled to establish the conditions of stimulation creating a population of cells in­corporating a large number of sensitizing molecules.
14

Application of electrophoretic methods for detection of protein-porphyrin complexes

75%
Kowalska A., Kwiek P., Milosz E., Gondek G., Romiszewska A., Graczyk A., Podhajska A. J.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 4
Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel Doc™ 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-trans­ferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
15

The effect of photosensitizers on cell metabolism in vitro

75%
Kadziauskas J., Kirveliene V., Prasmickaite L., Juodka B.
Polish Journal of Environmental Studies
|
1998
|
tom 07
|
nr 6
16

Comparison of the photosensitivity of biofilms of different genera of cariogenic bacteria in tooth slices

63%
Darmani H., Tawalbeh K. H., Al-Hiyasat A. S., Al-Akhras M.-A.
Polish Journal of Microbiology
|
2018
|
tom 67
|
nr 4
This study compared the outcome of photosensitization on the viability of four different cariogens in planktonic form as well as biofilms in human dentine. Photodynamic therapy was carried out with a gallium aluminium arsenide laser (670 nm wavelength) using Toluidine blue O (TBO) as the photosensitizer. Cariogenic bacteria (Streptococcus mutans, Lactobacillus casei, Streptococcus salivarius and Actinomyces viscosus) were exposed to TBO and then to the laser for 1 minute in planktonic suspension. Then, tooth slices previously incubated for 24 hours with broth cultures of broth culture of the four cariogenic organisms were exposed to antimicrobial photosensitization. The control samples consisted of planktonic and sessile cells that were exposed to TBO alone, laser alone and the bacterial cells that were not treated with TBO or laser. The results showed significant reductions in the viability of S. mutans, L.casei and A. viscosus in both planktonic form (to 13%, 30%, and 55%, respectively) and sessile form hosted in dentinal tubules (to 19%, 13% and 52%, respectively), relative to the controls. S.salivarius was the least affected in planktonic (94% viability) and sessile form (86% viability). In conclusion, sensitivity to photosensitization is species-dependent and sessile biofilm cells are affected to the same extent as their planktonic counterparts.
17

Cytotoxicity of PP(Arg)2- and Hp(Arg)2-mediated photodynamic therapy and early stage of apoptosis induction in prostate carcinoma in vitro

63%
Nowak-Stepniowska A., Wiktorska K., Malecki M., Romiszewska A., Padzik-Graczyk A.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 4
Porphyrin photosensitizers tend to localize in mitochondria. The depolarization of mitochondrial membrane is one of the early stages of apoptosis and Laser Scanning Fluorescence Microscopy allows to determine changes in transmembrane mitochondrial potential under influence of PDT depending on the kind of photosensitizer (PP(Arg)2, Hp(Arg)2), the energy dose (5, 10, 30 and 50 J/cm2) and time periods (24 and 48 hours after irradiation) in the LNCaP (lymphonodal metastasis of prostate carcinoma, the androgen dependent cell line). Cyototoxicity induced by PP(Arg)2- and Hp(Arg)2-based PDT depending on energy dose and time after irradiation in prostate carcinoma is determined with MTT. Generally, it was shown that lower energy doses induce greater changes in transmembrane mitochondrial potential. Hp(Arg)2-based PDT was more effective causing greater mitochondrial membrane depolarization and cell viability decrease in comparison to PP(Arg)2-mediated PDT (in the case of maximal nontoxic photosensitizer doses used).
18

Pulmonary metastases of the A549-derived lung adenocarcinoma tumors growing in nude mice. A multiple case study

63%
Jakubowska M., Sniegocka M., Podgorska E., Michalczyk-Wetula D., Urbanska K., Susz A., Fiedor L., Pyka J., Plonka P. M.
Acta Biochimica Polonica
|
2013
|
tom 60
|
nr 3
 Lung adenocarcinoma is a leading human malignancy with fatal prognosis. Ninety percent of the deaths, however, are caused by metastases. The model of subcutaneous tumor xenograft in nude mice was adopted to study the growth of control and photodynamically treated tumors derived from the human A549 lung adenocarcinoma cell line. As a side-result of the primary studies, observations on the metastasis of these tumors to the murine lungs were collected, and reported in the present paper. The metastasizing primary tumors were drained by a prominent number of lymphatic vessels. The metastatic tissue revealed the morphology of well-differentiated or trans-differentiated adenocarcinoma. Further histological and histochemical analyses demonstrated the presence of golden-brown granules in the metastatic tissue, similar to these found in the tumor tissue. In contrast to the primary tumors, the electron paramagnetic resonance spectroscopy revealed no nitric oxide - hemoglobin complexes (a source of intense paramagnetic signals), in the metastases. No metastases were found in other murine organs; however, white infarctions were identified in a single liver. Taken together, the A549-derived tumors growing subcutaneously in nude mice can metastasize and grow on site in the pulmonary tissue. Thus, they can represent an alternative for the model of induced metastatic nodule formation, following intravenous administration of the cancerous cells.
19

Cancer antineovascular therapy

63%
Oku N., Asai T., Kurohane K., Watanabe K., Kuromi K., Namba Y., Ogino K., Taki T.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr 2
20

Uptake of photofrin II, a photosensitizer used in photodynamic therapy, by tumour cells in vitro

63%
Chwilkowska A., Saczko J., Modrzycka T., Marcinkowska A., Malarska A., Bielewicz J., Patalas D., Banas T.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 2
Photosensitizing dyes are used in fluorescence diagnostics and photodynamic ther­apy (PDT). These usually hematoporphyrin derivatives (HpD) accumulate preferen­tially within neoplastic tissues. HpD is a mixture of ether and ester linked porphyrins. Its partially purified form is known under the commercial name of photofrin II (PII). PII emission spectra were studied in a hydrophilic (PBS) and a lipophilic (PC liposomes) environment. Red shift was observed in their emission maxima from 615 nm in buffer solution to 635 nm in lipid. Identical red shift was obtained when the intracellular fluorescence of two cancer cell lines, MCF 7 and Jurkat, incubated with PII was investigated. Thus, intramembrane localization of PII may be suggested. As determined from the total fluorescence intensity, the uptake of PII was only slightly higher for Jurkat than for MCF 7 cells. Nevertheless the kinetics of the uptake was found to be different for both cell lines.
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