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The essential oil extracts and antioxidant measurements of Syrian Myrtus communis L. leaves as hydrophilic and hydrophobic existence species have been carried out. The plant leaves as a source of antioxidants was tested by the influence of its aqueous and essential oil extracts on the yield of PCL solution applying very sensitive and reliable method. By means of a PCL assay, it was possible to assess the total antioxidants capacity of hydrophilic and hydrophobic species existence in Syrian Myrtus communis L. leaves. It has been found that the integral antioxidant capacity value of Syrian Myrtus communis L. leaves was found in Kurdaha site which has a value of 465.67±1.18 TE/g DM. The following three main substances were found in the essential oil extracts: a-pinene, cineole and limonene.
The antioxidant activity of rutin and selected common buckwheat-originated materials, namely groat, hull, flour and sprouts were measured against stable, non-biological radicals such as 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (ABTS•+) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) using a spectrophotometric assay, against the key reactive oxygen intermediate – superoxide anion radical (O2 –•) with a photochemiluminescence assay (PCL) while reducing capacity was determined with the cyclic voltammetry method (CV). The antioxidant activity was presented independently of the techniques used as TEAC values and then the contribution of rutin to the antioxidant capacity of buckwheat material was calculated. The order of the antioxidant activity of rutin provided by the updated analytical strategy was as follows: CV = DPPH RSA > PCL > ABTS RSA. Buckwheat groat and hull represented low antioxidant capacity samples whilst that of sprouts was ranked as high antioxidant capacity one. The results showed a low contribution of rutin to the antioxidant capacity of buckwheat groat and hull. The highest contribution of rutin to the antioxidant capacity was noted in buckwheat sprouts, especially those produced in light. The specificity of the rank of methods used as CV = DPPH RSA > ABTS RSA > PCL was concluded since the lowest antioxidant gap was provided with CV and DPPH RSA whilst the highest one with ABTS RSA and PCL.
The Syrian Thymus cilicicus (Th. cilicicus) Boiss & Bal. and Thymus syriacus (Th. syriacus) Boiss chemical components were identified using GC-MS spectrometry. Sixteen constituents representing an average of 93.85% of the essential oil from Syrian Thymus cilicicus were characterized. The major average components in four collection rounds of Thymus cilicicus around the year were: thymol, carvacrol, γ-terpinene, carvyl acetate, dihydrocarvone and anis aldehyde. Eighteen components representing an average of 93.46% of the essential oil of Thymus syriacus were characterized. The major average components in four collection rounds of Thymus syriacus around the year were: carvacrol, dihydrocarvone, β-caryophyllene, p-cymene, farnesol, limonine, menthol, myrecene, γ-terpinene, terpinene- 4-ol and thymol. The integral antioxidant capacity of aqueous and essential oils extracts of both Thymus species: Th. syriacus and Th. cilicicus have been determined by means of a photochemiluminescence assay (PCL). The highest integral antioxidant capacity has been found for Th. syriacus and Th. cilicicus in Fahel mountain and Salah Aldin locations which have a value of total nmol equivalent per gram of dry material at 374.6 ± 0.94 and 475.80 ± 1.20 nmol TE/g DM, respectively.
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