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The colour pigments of paprika (Capsicum annuun) were separated by reversed-phase high-performance liquid chromatography (HPLC) using normal (4 mm I.D.) and microbore (2 mm I.D.) C18 silica columns and gradient elution (water-acetonitrile-methanol). The relative standard deviation of retention times (lower than 1.5%) and that of peak areas (3-5%) were similar for both columns proving the good stability of the HPLC system. The high standard deviation of peak areas may be due to the presence of overlapping peaks. The number of separated pigment fractions was considerably higher on microbore (75) than on normal ODS column (34) proving the superiority of microbore column.
The aim of the research was to make a preliminary determination of the effectiveness of the in duc tion of haploids in Capsicum frutescens L. In order to induce androgenesis red and yellow fruit forms of species were used, each bred by the re searchers on their own. The experiment was per formed in October. An ther cultures were conducted according to a modified me - thod developed by Dumas et al. (1981) for C. annuum L. The anthers were laid on CP medium con taining 0.01 mg·dm⁻³ 2.4-D and 0.01 mg·dm⁻³ kinetin, with the addition of 0.5 g·dm⁻³ of activated carbon and 5 mg×dm⁻³ of silvernitrate, solidified with 8 g·dm⁻³ of agar. The cul tures were in cu bated in the dark at 35 deg C for 8 days. Next they were trans ferred to 25 deg C under a 12-hour photoperiod. After 14 days of induction, anthers were trans ferred to R₁ medium supplemented with 0.1 mg·dm⁻³ kinetin. Obtained embryos were subsequently transplanted onto V₃ hormone-free me dium and well growing plants were planted in green houses. The efficiency of androgenesis for both C. frutescens L. forms was relatively low and it did not exceed 5 %. The ploidy level of the result ing plants was determined by flow-cytometric analysis. The regenerants con sisted of about equal numbers of haploids and diploids. Additionally, among plants regenerated from anthers of yellow fruit forms, two mixoploids were observed.
In this study, the effect of arbuscular mycorrhizal fungus − AMF Rhizophagus intraradices inoculum (prior or with transplanting) to different pepper type (Capsicum annuum L.) cv. Arlequin F1 (long fruits) and Raiko F1 (bell pepper), on plant growth and physiological parameters in response to elevated soil P concentrations from organic greenhouse production with enhanced soil salinity, was investigated. To explain the physiological growth of mycorrhizal inoculated (M) and non-mycorrhizal inoculated (NM) plants, the parameters of fungal root length colonization, shoot concentration of P an N during growth, plant height, width of stem, yield, number of fruit per plant and also the quality parameters of fruits such as soluble solid content (SSC), fruit color, mineral profile, total soluble phenolics (TSP) and antioxidant activity (FRAP), were determined. This study showed that application of AMF in cv. Raiko cultivated in high P saline soil generally enhanced growth, fruit yield and number of fruits per plant when inoculated at planting time in the greenhouse. AM inoculated plants, regardless of the time of application in cv. Arlequin grown under the same conditions, did not have any significant differences in comparison with NM plants. Arbuscular mycorrhizal inoculation has great potential in enhancing the pepper growth and yield even in high soil P, however, because of the complexity and interaction of involved genotypes of pepper and AMF, the method and time of inoculation, the system of pepper production and environmental conditions, as well as assays have to be performed to verify positive effects.
The effect of three foliar-applied Ca-containing preparations on the anatomical features of leaves of sweet pepper (Capsicum annuum L.) was studied. The following preparations were used: Ca(NO3)2, Librel Ca and Wapnowit, applied at the respective concentrations of 0.5%, 1%, 1%, which corresponded to a content of 2000 mg Ca 􀂉 dm-3. Light and scanning electron microscopy were used in the study. It was demonstrated that in amphistomatic bifacial pepper leaves numerous specialised cells occurred which accumulated calcium oxalate crystals in the form of crystalline sand. Anisocytic stomata were found with a much greater density in the abaxial epidermis. They were characterized by very well-developed outer cuticular ledges. It was found that in the leaves of the plants sprayed with the nutrient supplements with increased Ca content there was a much smaller number of epidermal cells per 1 mm2 than in the control plants. These cells were distinguished by an increased size. In the case of the application of the nutrient supplements Librel Ca and Wapnowit, the number of stomata also decreased. However, the application of the calcium supplements resulted in an increase in the value of the stomatal index compared to the control, which is attributable to a significant reduction in the number of epidermal cells not belonging to the stomata. The plants additionally supplied with Ca were marked by a larger number of colenchyma layers and an increased volume of leaf parenchyma cells. In the case of pepper leaves, the thin cuticle and the outer cell wall are not a major barrier to the Ca-containing preparations applied for spray treatment. Nevertheless, the decrease in the number of stomata may restrict the possibility of Ca uptake by this way, which compensates the increase in surface area of particular epidermal cells that will be the main way of Ca penetration into the internal leaf tissues.
Haploid plants after doubling the chromosomes can give rise to diploid homozygote lines, which can be used as DH lines in breeding new varieties or as initial plant material in creating F1 hybrids. This work studied natural polyembryony and the effect of growth regulators on induction of polyembryonic seeds and haploid embryogenesis in five species of the genus Capsicum. Water solutions of the following growth regulators were used: 2,4-D (2,4-dichlorophenoxyacetic acid) and BNOA (beta-naphthoxyacetic acid) at 0.001% used separately or combined with BAP (benzylamino-purine). Twin seed frequency was highest in C. chinense and lowest in C. baccatum var. pendulum. In C. annuum the share of twin embryos was highest in the 'Corno di toro' variety; 2,4-D clearly increased the number of twin plants only in 'Corno di toro' seeds. Treatment with combinations of 2,4-D or BNOA with BAP increased the frequency of polyembryonie seeds in the 'ATZ1' line. In C. frutescens the frequency of polyembryony increased following application of BNOA with BAP. Of all the seeds tested, seven haploid plants were obtained, representing C. frutescens, C. chacoense and C. baccatum var. pendulum. The differences in the frequency of polyembryony in the studied genotypes points to genetic control of this phenomenon. The presence of monoploid plants definitely depends on the genotype, as a high frequency of polyembryony is not always accompanied by a high share of haploid plants. For most genotypes evaluated, the effect of growth regulators was disadvantageous, resulting in a considerable decrease in the share of twin plants among germinated seeds.
The aim of the present study was to determine va-rious physical, mechanical, and structural characteristics of seed of pepper cultivars Yağlık Kapya, Demre Sivri, Yalova Carliston, Kandil Dolma, and Cin Sus Yediveren, which are grown extensi-vely in Turkey, with initial moisture content ranging between 7.03 and 7.21% (w.b.). Width, length, and thickness were found to be in the range of 3.00-3.72, 3.43-4.40, and 0.66-0.82 mm, respectively. It was revealed that sphericity of the seeds of Yağlık Kapya, Demre Sivri, Yalova Carliston, Kandil Dolma, and Cin Sus Yediveren were 51, 57, 56, 51 and 57%, respectively. In terms of the roundness which ranges between 76 and 85% depending on pepper cultivars. The aspect ratio of Yağlık Kapya, Demre Sivri, Yalova Carliston, Kandil Dolma, and Cin Sus Yediveren were determined to be 84.60, 88.04, 94.43, 85.55, and 87.67%, respectively.It was also noted that the porosity of the pepper seeds was in the range of 44.94-49.61%. Besides, we found that as the weight of pep-per seeds increased, their terminal velocity increased accordingly, and thus terminal velocities were found to be 2.87-4.66 m s-1. In the current study, the static friction angle and coefficients of the cultivars were determined by means of six different plates including aluminium, stainless steel, galvanized iron, rubber, glass, and plywood. The plywood plate was found to be the least slippery; consequently, the static friction angle and coefficient were determined to be the highest for the plywood plate.
The effects of various technological steps and raw material inhomogeneity on the composition of pigments in paprika (Capsicum annuum) powders were studied by reversed-phase high-performance liquid chromatography (RP-HPLC), using gradient elution technique (various mixtures of water, acetonitrile and methanol) and diodearray detector. The standard deviation of the composition of pigments in the samples taken at different technological stages was lower than among the batches of raw material. This indicates that the technological steps have a negligible effect on the composition of pigments in paprika powder. It was further found that the raw material for the paprika powder manufacture could considerably be inhomogeneous even when harvested on the same field.
The study-compared effect of cytokinins on morphogenesis and ploidy of plants regenerated in vitro from explants of ‘Stanola F1’, ATZ and ATM pepper seeds. The aim of defining the morphogenetic potential of the studied genotypes, half-seed explants consisting the proximal part of the hypocotyl and radicle were put onto the MS medium containing BAP (5.0 mg.dm-3), 2iP (2.5 mg.dm-3), ZEA (2.5 mg.dm-3), TDZ (1.5 mg.dm-3), while MS medium without cytokinins constituted the control. After the initiation period, explants were transferred onto the medium without cytokinins. The effect of the growth regulators to the morphogenetic response of explants was estimated based on the number of explants on which adventitious buds and shoots were developing. The cytokinins applied did not show a significant effect on the development of adventitious buds on pepper explants.
The effect of ethephon applied at seedling on accumulation of earotenoid pigments in fruit of hot pepper Capsicum annuum L. var Bronowicka Ostra was studied. Ethephon was found to stimulate fruit maturation. The mass of ripe fruit from the second and third harvest increased by more than 44% on average compared to controls. Fruit from the third harvest had higher content of red pigment capsanthin (11% on average) and ß-earotene (14%), and ß-cryptoxanthin-provitamin A (18%) than control fruit. The increase in the level of these pigments was accompanied by a decrease in the amount of zeaxanthin and disappearance of neoxanthin. Supposedly ethephon regulates fruit maturation by stimulating carotenogenesis.
In order to assess the economic benefits of reduced fungicide application for the control of paprika diseases under dryland conditions, on-farm experiments were conducted in the Chinyika Resettlement Area in the Eastern province of Zimbabwe in the 2000/2001 and 2001/2002 seasons. The six fungicide application regimes that were assessed include: weekly interval sprays; Sulphur at 2 weeks after transplanting (WAT) and copper oxychloride-Mancozeb mixture at 6 WAT; spraying after scouting; alternating Sulphur and copper oxychloride- Mancozeb every two weeks; Acibenzolar-s-methyl and unsprayed check. Parameters recorded were disease severity and yield; after harvest an economic analysis was performed. The highest added profit of Z$ 75930/ha was recorded in the weekly sprayed plots which was not statistically different (p > 0.05) from Z$ 59410/ha achieved by alternating copper oxychloride and Mancozeb fortnightly at Dengedza site in 2000/2001 season. There were no statistical differences (p > 0.05) between spraying after scouting and Acibenzolar-s-methyl application treatments as they added the least profits of Z$ 990/ha and Z$ 17250/ha respectively at the same site in the same season. These have serious implications for smallholder farmers in terms of cost savings. Neither were there differences (p > 0.05) in added profits from different spraying regimes at Dengedza site in the 2001/2002 rainy season.
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