Wyniki wyszukiwania

1

Biochemical mechanisms of apoptotic execution

100%

Ruchaud S., Samejima K., Durrieu F., Earnshaw W.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

2

Children brain weight in various pathological conditions

100%

Kraszpulska B.

Folia Morphologica

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1996

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tom 55

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nr 4

3

Ceramides and sphingosine-1-phosphate in molecular mechanisms of neuronal cell death

86%

Czubowicz K., Strosznajder R. P.

Acta Neurobiologiae Experimentalis

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2013

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tom 73

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nr 1

Ceramides, bioactive members of the sphingolipids can be generated by de novo synthesis, sphingomyelin hydrolysis and by acylation of sphingosine. Ceramides are known to regulate several cellular processes, including differentiation, growth suppression, cell senescence and apoptosis. The ceramide levels increased in several pathological conditions such as brain ischemia, hypoglycemia, inflammation and in neurodegenerative disorders. Sphingosine, a metabolite of ceramide is phosphorylated by sphingosine kinases (Sphk type 1and 2) to sphingosine-1-phosphate (S1P). Sphingosine kinases are critical regulators of the sphingolipid biostat. The aim of this study was to investigate the role of ceramide and S1P in molecular mechanisms of neuronal cells death. The human neuroblastoma cell line (SH-SY5Y) was exposed to cell-permeable C2-ceramide. Ceramide decreased the viability of SH-SY5Y cells in concentration dependent manner. The intracellular free radical generation after ceramide treatment was about 3-fold higher comparing to control. Concomitantly our study indicated that ceramide induced poly(ADP-ribose) polymerase-1 (PARP-1) activation and decreased the level of apoptosis inducing factor (AIF) in mitochondria. Ceramide diminished the expression and level of anti-apoptotic Bcl-2 protein. PARP-1 inhibitor enhanced the level of Bcl-2 protein and cells survival keeping the level of AIF in mitochondria unchanged. The recent studies indicated that ERK1/2 are involved directly in regulation of PARP-1 activity. The specific inhibitor of these kinases protected cells against death evoked by ceramide in our experimental conditions. Moreover, our study indicated, that sphingosine-1-phosphate (S1P) increased Bcl-2 gene expression and SH-SY5Y cells survival after ceramide treatment. Summarizing, our data present that PARP-1 inhibitor and sphingosine-1-phosphate (S1P) through modulation of anti-apoptotic proteins protect mitochondria and neuronal cells against death evoked by ceramide. Supported by statutory budget of MRC and NCN Grant 5870/PO1/2011/40

4

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Local immunity of the respiratory mucosal system in chickens and turkeys

86%

Smialek M., Tykalowski B., Stenzel T., Koncicki A.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 2

This review article presents fundamental mechanisms of the local mucosal immunity in selected regions of the respiratory tract in healthy birds and in some pathological conditions. The respiratory system, whose mucosa come into direct contact with microorganisms contaminating inhaled air, has some associated structures, such as Harderian gland (HG), conjunctive-associated lymphoid tissue (CALT) and paranasal glands (PG), whose participation in local mechanisms of the mucosal immunity has been corroborated by numerous scientific studies. The nasal mucosa, with structured clusters of lymphoid tissue (NALT – nasal-associated lymphoid tissue) is the first to come into contact with microorganisms which contaminate inhaled air. Lymphoid nodules, made up of B cells with frequently developed germinal centres (GC), surrounded by a coat of CD4+ cells, are the major NALT structures in chickens, whereas CD8+ cells are situated in the epithelium and in the lamina propria of the nasal cavity mucosa. Studies into respiratory system infections (e.g. Mycoplasma gallisepticum) have shown the reactivity of the tracheal mucosa to infection, despite a lack of essential lymphoid tissue. Bronchus-associated lymphoid tissue (BALT) takes part in bronchial immune processes and its structure, topography and ability to perform defensive function in birds is largely age-dependent. Mature BALT is covered by a delicate layer of epithelial cells, called follicle-associated epithelium (FAE). Germinal centres (GC), surrounded by CD4+ cells are developed in most mature BALT nodules, while CD8+ lymphocytes are dispersed among lymphoid nodules and in the epithelium, and they are rarely present in GC. Macrophages make up the first line of defence mechanisms through which the host rapidly responds to microorganisms and their products in the respiratory mucosal system. Another very important element are polymorphonuclear cells, with heterophils being the most important of them. Phagocytic cells obtained from lung lavages in birds are referred to as FARM (free avian respiratory macrophages). Their number in chickens and turkeys is estimated to be 20 times lower than that in mice and rats, which indicates a deficit in the first-line of defence in the birds’ respiratory system. There are numerous B cells and antibody secreting cells (ASC) present throughout the respiratory system in birds. Their role comes down to perform antigen-specific protection by producing antibodies (IgM, IgY or IgA class) as a result of contact with pathogenic factors.

5

Clinical and pathological aspects of canine cutaneous leishmaniasis: a meta-analysis

86%

Sobotyk Oliveira C., Ratzlaff F. R., Potter L., Romao P. R. T., de Avila Botton S., Vogel F. S. F., Sangioni L. A.

Acta Parasitologica

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2019

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tom 64

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nr 4

6

Fluorescence and spin-labelling as the techniques of choice for studying the dynamics of platelet membranes and their interactions under pathological conditions

86%

Watala C., Gwozdzinski K., Pietrucha T., Cierniewski C. S.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

7

Bim: guardian of tissue homeostasis and critical regulator of the immune system, tumorigenesis and bone biology

72%

Akiyama T., Tanaka S.

Archivum Immunologiae et Therapiae Experimentalis

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2011

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tom 59

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nr 4

8

Influence of Echinacea purpurea intake during pregnancy on fetal growth and tissue angiogenic activity

72%

Barcz E., Sommer E., Nartowska J., Balan B. J., Chorostowska-Wynimko J., Skopinska-Rozewska E.

Folia Histochemica et Cytobiologica. Supplement

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2007

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tom 45

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nr 1

35-39

9

A new membrane G protein-coupled receptor (GPR30) is involved in the cardiac effects of 17beta-estradiol in the male rat

72%

Filice E., Recchia A. G., Pellegrino D., Angelone T., Maggiolini M., Cerra M. C.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 4

3-10

In the present study, we evaluated the transduction pathways involved in the cardiac effects elicited by 17ß-estradiol (E2) on the isolated, Langendorff perfused male Wistar rat heart. E2 and selective agonists for ER and ERß induced a dose-dependent reduction of contractility which was blocked by the ER inhibitor ICI 182,780. Moreover, the potential involvement of the novel membrane estrogen receptor GPR30 in mediating estrogen activity was determined using the selective GPR30 ligand G-1. Notably, specific inhibitors of ERK, PI3K, PKA, and eNOS transduction pathways abolished the cardiac responses to E2. Taken together, our data suggest that ER and ERß along with several signaling cascades are involved in the action of E2 on the male rat heart. Our results also point to a potential role of GPR30, however further evaluation is required in order to fully understand the contribution of the different estrogen receptors in mediating estrogen activity on cardiac performance.

10

Role of cathepsin A and cathepsin C in the regulation of glycosidase activity

72%

Minarowska A., Minarowski L., Karwowska A., Milewska A. J., Gacko M.

Folia Histochemica et Cytobiologica

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2012

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tom 50

|

nr 1

11

Two separate cases of extrauterine pregnancy in queens

72%

Dzieciol M., Nizanski W., Ochota M., Blasiak K., Kozdrowski R., Stanczyk E., Twardon J.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2012

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tom 15

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nr 2

12

The impact of hypertonic saline inhalation on mucociliary clearance and nasal nitric oxide

72%

Bencova A., Vidan J., Rozborilova E., Kocan I.

Journal of Physiology and Pharmacology

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2012

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tom 63

|

nr 3

13

Oxygen uptake kinetics: Why are they so slow? And what do they tell us?

72%

Grassi B.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

|

nr 10

53-65

O2 kinetics and O2 deficit are important determinants of exercise tolerance. In "normal" conditions convective and diffusive O2 delivery to skeletal muscle fibers do not represent important determinants of O2 kinetics, whose limiting factors seem mainly located within muscle fibers. Whereas a limiting role by PDH has not been confirmed, the role of inhibition of mitochondrial respiration by NO needs further investigations. Important determinants of skeletal muscle O2 kinetics likely reside in the interplay between bioenergetic mechanisms at exercise onset. By acting as high-capacitance energy buffers, PCr hydrolysis and anaerobic glycolysis would delay or attenuate the increase in [ADP] within muscle fibers following rapid increases in ATP demand, preventing a more rapid activation of oxidative phosphorylation. The different "localization" of the main limiting factors for O2 kinetics and O2max offers the opportunity to perform a functional evaluation of oxidative metabolism at two different levels of the pathway for O2, from ambient air to mitochondria. WhereasO2max is mainly limited by the capacity of the cardiovascular system to deliver O2 to exercising muscles, by analysis of O2 kinetics the functional evaluation is mainly related to skeletal muscle. In pathological conditions the situation may be less clear, and warrants further investigations.

14

Red blood cell damage in chronic renal failure

72%

Gwozdzinski K.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

15

Pyridine nucleotide concentration and activity of related enzymes in human lymphocytes

72%

Sestini S., Jacomelli G., Pescaglini M., Micheli V., Pompucci G.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

16

Transcriptional regulation in thymic epithelial cells for the establishment of self tolerance

72%

Matsumoto M.

Archivum Immunologiae et Therapiae Experimentalis

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2007

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tom 55

|

nr 1

17

Thyroid hormone receptor alpha1: a switch to cardiac cell 'metamorphosis'?

72%

Pantos C., Xinaris C., Mourouzis I., Perimenis P., Politi E., Spanou D., Cokkinos D. V.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 2

Thyroid hormone receptor alphalpha1 (TRalpha1) is predominantly expressed in the myocardium but its biological function under physiological or pathological conditions remains largely unknown. The present study investigated possible interactions between alpha1 adrenergic and thyroid hormone signaling at the level of TRalpha1, potential underlying mechanisms and physiological consequences, as well as the role of TRalpha1 in cell differentiation. This may be of physiological relevance since both thyroid hormone and adrenergic signalling are implicated in the pathophysiology of cardiac remodelling. Neonatal cardiomyocytes obtained from newborn rats (2-3 days) were exposed to phenylephrine (PE, an alpha1 adrenergic agonist) for 5 days, in the absence or excess of T3 in the culture medium. PE, in the absence of T3, resulted in 5.0 fold increase in TRalpha1 expression in nucleus and 2.0 fold decrease in TRalpha1 expression in cytosol, P<0.05. As a result, a fetal pattern of myosin isoform expression with marked expression of ß–MHC was observed in PE treated vs the untreated cells, P<0.05. PD98059 (an ERK signalling inhibitor) abrogated this response. In the presence of T3 in the culture medium, TRalpha1 expression was increased 1.6 fold in nucleus and 2.0 fold in cytosol in PE-T3 vs PE treated cells, P<0.05, and the fetal pattern of myosin isoform expression was prevented. Parallel studies with H9c2 myoblasts showed that reduction of T3 binding to TRalpha1 receptor delayed cardiac myoblasts differentiation without affecting proliferation. In conclusion, in neonatal cardiomyocytes, nuclear TRalpha1 is overexpressed after prolonged activation of the alpha1- adrenergic signalling by PE. This response seems to be an ERK kinase dependent process. Over-expression of TRalpha1 may lead to fetal cardiac phenotype in the absence of thyroid hormone availability. Furthermore, TRalpha1 seems to be critical in cardiac myoblast differentiation.

18

The slow sarco-endoplasmic reticulum Ca2plus-ATPase declines independently of slow myosin in soleus muscle of diabetic rats

58%

Racz G., Szabo A., Ver A., Zador E.

Acta Biochimica Polonica

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2009

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tom 56

|

nr 3

487-493

The sarcoplasmic reticulum Ca2+-ATPase (SERCA) isoforms are normally expressed in coordination with the corresponding myosin heavy chain (MyHC) isoforms in the fibers of skeletal muscle but this coordination is often disrupted in pathological conditions. In the streptozotocin-induced diabetes of rats (stz-rats), the soleus muscle showed peripheral neuropathy and the SERCA2a level decreased in type I (slow-oxidative) fibers compared to the control muscles, whereas the expression of the corresponding slow MyHC1 did not change. No difference was found at the mRNA and protein levels of SERCA and MyHC isoforms in the whole soleus, except that the level of the SERCA2a protein specifically declined in stz-rats compared to the controls. This shows that the coordinated expression of SERCA2a and MyHC1 is disrupted at the SERCA2a protein level in the diabetic soleus. The results are in line with previous observations that regulators of the Ca-homeostasis may adapt faster to type I diabetes than the contractile elements.

19

Relations between iron deficiency anemia and serum levels of copper, zinc, cadmium and lead

58%

Turgut S., Hacioglu S., Emmungil G., Turgut G., Keskin A.

Polish Journal of Environmental Studies

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2009

|

tom 18

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nr 2

273-277

Iron deficiency anemia (IDA) is a common nutritional deficiency syndrome. Lead and cadmium are major hazard elements to humans in industrialized countries. Zinc and copper are essential and play important roles in different physiologic and pathologic conditions. The aim of our study was to determine levels of serum Cu, Zn, Fe, Cd and Pb in IDA patients, and to investigate the relationship between these elements and IDA. This study was performed on 141 adults (age 18-40 years) living in the Denizli region of Turkey. Iron deficiency anemia was observed in 81 individuals; 60 healthy persons (without anemia) were regarded as controls. Blood samples were collected from subjects into without anticoagulant tubes free from Fe, Cu, Zn, Cd, Pb and sera were obtained. Element levels of serum were determined using an atomic absorption spectrophotometer. The study took place in the Pamukkale University Faculty of Medicine in 2005. The levels of lead in serum were significantly (p<0.001) higher in adults with IDA than controls. Serum copper and zinc concentration of the IDA group were not found to significantly (p>0.05) differ from the control group. Cadmium level in IDA group appeared to be higher than control, but not significantly (p>0.05) different from that of the control group. Hemoglobin, mean corpuscular volume, red blood cell, ferritin and iron levels in subjects with IDA were significantly (p<0.001) lower than control. Serum lead concentration is high in IDA subjects versus healthy individuals. So it can be said that lead exposure may be a risk factor for the occurrence of iron deficiency anemia in humans. In addition, it can be said that iron deficiency may increase susceptibility to lead poisoning because it has been speculated that iron deficiency can cause increased absorption of lead.

20

Fas/FasL expression in colorectal cancer. An immunohistochemical study

58%

Pryczynicz A., Guzinska-Ustymowicz K., Kemona A.

Folia Histochemica et Cytobiologica

|

2010

|

tom 48

|

nr 3

Ograniczanie wyników

Biochemical mechanisms of apoptotic execution

Children brain weight in various pathological conditions

Ceramides and sphingosine-1-phosphate in molecular mechanisms of neuronal cell death

Local immunity of the respiratory mucosal system in chickens and turkeys

Clinical and pathological aspects of canine cutaneous leishmaniasis: a meta-analysis

Fluorescence and spin-labelling as the techniques of choice for studying the dynamics of platelet membranes and their interactions under pathological conditions

Bim: guardian of tissue homeostasis and critical regulator of the immune system, tumorigenesis and bone biology

Influence of Echinacea purpurea intake during pregnancy on fetal growth and tissue angiogenic activity

A new membrane G protein-coupled receptor (GPR30) is involved in the cardiac effects of 17beta-estradiol in the male rat

Role of cathepsin A and cathepsin C in the regulation of glycosidase activity

Two separate cases of extrauterine pregnancy in queens

The impact of hypertonic saline inhalation on mucociliary clearance and nasal nitric oxide

Oxygen uptake kinetics: Why are they so slow? And what do they tell us?

Red blood cell damage in chronic renal failure

Pyridine nucleotide concentration and activity of related enzymes in human lymphocytes

Transcriptional regulation in thymic epithelial cells for the establishment of self tolerance

Thyroid hormone receptor alpha1: a switch to cardiac cell 'metamorphosis'?