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Plants respond to infection by accumulating many compounds some of which may function in disease resistance. These include: phytoalexins, antifungal proteins, chitinases, glucanases, esterases, proteaes, phospholipases, lipoxygenases, ribonucleases, peroxidases, phenoloxidases, lignin, callose, hydroxyproline and glycine-rich glycoproteins, phenolic cross-linked polysachcarides, melanin-like pigments, salicylic acid, jasmonic acid, ethylene, peptides, oligosaccharides, hydrogen peroxide and active oxygen species. Though specific avirulence genes, elicitors and elicitor receptors have been reported, the production of defense-related compounds is nonspecific and can be elicited by pathogens, pathogen products and many organics and inorganics. The molecular implications of this specificity/nonspecificity and their significance to disease resistance and practical disease control will be discussed.
The Shiga toxin-producing Escherichia coli (STEC) strains are currently considered important emerging pathogens threatening public health. Among Shiga toxin-producing Escherichia coli, E. coli O157:H7 strains have emerged as important human pathogens. This study was conducted to determine the presence of Escherichia coli O157 and O157:H7 in raw milk samples and Van herby cheese samples. For this purpose, 100 samples of raw milk were collected and 100 samples of herby cheese sold for consumption in Van province in Turkey were obtained from grocers and markets in order to detect the presence of Escherichia coli O157 and O157:H7. The method of E. coli O157 and O157:H7 isolation proposed by the Food and Drug Administration (FDA) was used. E. coli O157 in raw milk and herby cheese samples was found in 11% and 6% of samples respectively, and E. coli O157:H7 was found in 2% of herby cheese samples. No E. coli O157:H7 was detected in raw milk samples. This study showed that raw milk was contaminated with E. coli O157 and herby cheese was contaminated with both E. coli Ol 57 and E. coli Ol57:H7; therefore, herby cheese poses a serious risk to public health.
Since C. pseudotuberculosis is a facultative intracellular pathogen the aim of this study was focused on evaluating mechanisms that allowed these bacteria to survive in macrophages and determining their influence on induction of cell death. The influence of Corynebacteria on the programmed cell death of macrophages was determined on the basis of induction the autophagy and apoptosis in the cultures of murine macrophage cell lines J774 infected with bacteria. Corynebacterium pseudotuberculosis strains could survive within macrophages more than 48 hours. During that time bacteria were released as a result of the process that lead to death of phagocytes. This property varied among studied strains. There was no increase of micro- tubule-associated protein I light chain 3 (MAP I LC3) activity in macrophages infected with examined strains comparing with uninfected cultures and cultures treated with autophagy inducer (rapamycin) that served as negative and positive controls, respectively. The study with confocal microscopy did not show the increasing of caspase-3 activity in the infected macrophages and their nucleus did not reveal the fragmentation.
Similary to other fungal species of the genus Alternaria, Alternaria radicina is a major pathogen that infects both the aboveground and underground parts of carrot plants. Changes in the concentrations of phenolic compounds are observed in infected carrot plants. Carrot seedlings were inoculated with the most pathogenic isolates of Alternaria radicina selected in a laboratory test. A phytopathological analysis was performed to determine the health status of carrot plants. The concentrations of phenolic acids in petioles were determined four weeks after inoculation with A. radicina and at harvest. The results of a greenhouse experiment revealed more severe disease symptoms on carrot plants cv. Koral inoculated with A. radicina isolates, compared with cv. Bolero. The content of the predominant phenolic acid (chlorogenic acid) was found to decrease in the leaf stalks of carrots cv. Koral inoculated with A. radicina. A statistically non-significant increase in chlorogenic acid levels was noted in the leaf stalks of carrots cv. Bolero in the experimental and control groups.
Apple scar skin viroid (ASSVd) is a serious pathogen of apple fruits that results in severe loss in apple production. Up to nowadays, many ASSVd management options are applied to resist the disease, but the diserable resulits are not achieved. Therefore, this study was conducted in 2010–2012 at experimental field of Penglai City, Shandong Province, China (E 120°57’22’’, N 370°38’33’’) to investigate whether arbuscular mycorrhizal (AM) Glomus versiforme protects Red Fuji apple trees (Malus × domestica Borkh) against apple scar skin viroid. Red Fuji apple trees were inoculated with Glomus versiforme and then potential protection mechanism was explored and compared to noninoculated plants. The results showed that inoculation with Glomus versiforme significantly increased root length colonization rate and clearly decreased the percentage of disease severity of apple scar skin disease. Compared to non-inoculated plants, Glomus versiforme obviously enhanced total nitrogen and phosphorus concentrations in leaves. Root colonization by Glomus versiforme induced an increase in defense-related enzyme activities in fruits, such as the enhanced activities of catalase, ascorbate peroxidase, chitinase and glucanase. Significant differences in acid content of fruit and fruit yield were observed as apple roots were colonized by Glomus versiforme. It is therefore concluded that Glomus versiforme can be regarded as a biocontrol agent to protect apple trees against the infection with ASSVd.
Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of Sclerotinia stem rot, is one of the most important pathogens of Brassica napus L. in northern Iran. In this study, 13 mycelial compatibility groups (MCGs) of the fungus were identified among 31 isolates sampled from four regions of Mazandaran province, Iran. Effective fungicides are useful in the integrated management of the disease. So, the effect of tebuconazole, propiconazole, cyproconazole, and Rovral-TS at five doses (0.0001, 0.001, 0.01, 0.1, and 1 ppm) was studied on the growth inhibition of S. sclerotiorum as in vitro tests. Maximum inhibition (100%) of S. sclerotiorum mycelial growth was obtained by the highest dose (1 ppm) of all tested fungicides, as well as by the doses of 0.1 and 0.01 ppm of propiconazole, cyproconazole, and tebuconazole. In this investigation, the reaction of S. sclerotiorum isolates belonging to different MCGs was evaluated against tebuconazole, propiconazole, cyproconazole, and Rovral-TS at their EC50 ranges. The results revealed that there was high variation of S. sclerotiorum MCGs against different fungicides. The inhibition percentage varied between 4.29% and 71.72%.
Transition from round budding cells to long hyphal forms and production of secreted aspartic proteases (Saps) are considered virulenceassociated factors of Candida albicans. Although plenty of data dealing with Saps involvement in the infection process have been published, Saps expression by the different pleomorphic forms as well as the capacity of C. albicans filaments to express Sap1-6 under serum influence are poorly investigated. In this study, we used immunofluorescence and immunoelectron microscopy for the detection of Sap1-6 isoenzymes in C. albicans pleomorphic cells (blastoconidia, germ tubes, pseudohyphae, true hyphae) grown in Sap-inductive human serum and Sap non-inductive medium – yeast extract-peptone-glucose (YEPD). Isoenzymes were below the detection level in all blastoconidial cells grown in YEPD for 18 h. Sap1-6 expression was hardly detected in C. albicans cells cultivated in serum for 20 min. Increasing level of Sap1-6 expression was observed when C. albicans was incubated for 2, 6 and 18 h in serum corresponding to the development of germ tubes, pseudohyphae and true hyphae. !e expression of Sap1-3 in pseudohyphae and true hyphae was more intensive compared to Sap4-6. !us, we could show that human serum induced hyphae formation and the expression of Sap1-6 were co-regulated.
Between 2000 and 2006 the sum of ciprofloxacin and folic acid antagonists prescriptions to Bavarian (South-eastern Germany) outpatients stayed constant. However, prescription numbers of ciprofloxacin increased while those of folic acid antagonists decreased suggesting an apparent shift in the treatment of urinary infections toward ciprofloxacin. During the observation period the proportion of E. cbti resistant against ciprofloxacin increased from 5% to 10% while that against co-trimoxazole increased from 21 % to 27%. The proportion of E. coli simultaneously exhibiting resistance to ciprofloxacin and co-trimoxazole increased from 3.9% to 8.5%. A leading influence of ciprofloxacin application for these developments is discussed.
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