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1

Direct detection of Mycobacterium avium subsp. Paratuberculosis in bovine milk by multiplex real-time PCR

100%
Selim A., El-haig M., Galila E. S., Gaede W.
Animal Science Papers and Reports
|
2013
|
tom 31
|
nr 4
The study aimed at direct detection of Mycobacterium avium subsp. Paratuberculosis (MAP) in milk by evaluating a multiplex real-time PCR assay targeting IS900 and ISMAV2 sequences including the amplification of PUC19-plasmid as internal control. The sensitivity of the assays was evaluated by testing MAP isolates in broad linear range of DNA (50 ng – 5 fg/μl). For the validation of the specificity, 6 MAP isolates and 22 isolates of genus Mycobacteriaceae were tested.Results revealed that reproducible detection limit for real-time PCR targeting IS900 and ISMAV2 was 5 fg/μl and 50 fg/μl respectively. By targeting ISMAV2 sequence, 100% specificity was detected. However, a cross reaction with 5 ng/μl of genome of 3 M. avian subspecies avium strains was detected by targeting IS900 and negative in lower genome quantity (5pg/μl). To maximize the assay’s detection sensitivity,an efficient strategy for MAP-DNA extraction from spiked milk was assessed. Targeting of IS900 was sensitive and targeting ISMAV2 was very specific. Therefore, a multiplex real-time PCR assay targeting IS900 and ISMAV2 in combination with two commercial DNA extraction kits could be an ideal sensitive and specific protocol for routine large-scale analysis of milk samples and other clinical specimens from man and animals.
2

Predisposition to diseases and mutations in the genes of immunity system

100%
Mikula Sr. I., Bhide M., Mikula Jr. I., Kovac G.
Prace i Materiały Zootechniczne
|
2009
|
tom 67
3

Detection methods of Mycobacterium paratuberculosis

100%
Wiszniewska A., Szteyn J.
Polish Journal of Veterinary Sciences
|
2002
|
tom 05
|
nr 3
4

Paratuberculosis in cattle in Turkey detected by PCR

100%
Ikiz S., Bagcigil A. F., Ak S., Ozgur N. Y., Lgaz A.
Medycyna Weterynaryjna
|
2005
|
tom 61
|
nr 08
5

Agreement between ELISA and complement fixation test used for diagnosing of paratuberculosis in goats

67%
Kaba J., Gerlach G. F., Nowicki M., Rypula K.
Polish Journal of Veterinary Sciences
|
2008
|
tom 11
|
nr 3
209-212
An ELISA with a iipoarabinomannan as an antigen, developed for diagnosis of bovine paratuber- culosis, has been adapted for use in goats, and compared with complement fixation test. Kappa value of 0.62 indicated good agreement between CFT and the adapted ELISA and proved that the investigated ELISA may be helpful in diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats. The ELISA has been used to screen a randomly selected representative sample of Polish breeding goat population (21.78% of herds, 21.33% of goats). It has been demonstrated that only 2.42% of animals coming from 15.79% of herds were seropositive. Within-herd seroprevalence varied from 1.69% to 38.10%. Most of the infected animals (67.07%) were 3-4 years old. No seropositive cases were found in group up to 1 year old animals.
6

Paratuberkuloza: jak kontrolować chorobę?

67%
Bednarski M.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2015
|
tom 10
|
nr 1
7

Choroby zakaźne

67%
Gluchowski L.
Farmer
|
2018
|
nr 05
8

Changes in raw milk quality in herds infected with Mycobacterium avium subsp. paratuberculosis

67%
Fus-Szewczyk M. M., Szteyn J., Wiszniewska A.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 1
9

Analysis of correlation between the occurrence of anti-MAP antibodies in blood serum and the presence of DNA-MAP in milk

59%
Wiszniewska-Laszczych A., Szteyn J., Smolinska A.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 3
Paratuberculosis (Johne’s disease) is a chronic, infectious enteritis of both domestic and wild ruminants. Unfortunately, the problem of MAP infections is not linked only with the health status of animals and potential direct and indirect economic losses in bovine herds (of dairy cattle in particular). MAP bacilli present in food of animal origin (milk in particular) are likely to lead to the development od the disease in humans. Fast and effective diagnosis of the disease in animals, especially of its subclinical form, may prevent the transmission of the germ to humans. The study was aimed at analyzing the correlations between the occurance of seropositive and serodoubtful reaction in the ELISA test and the presence of DNA-MAP in udder milk. The results suggest that half of the population of animals with positive and doubtful serological responces against John’s disease are likely to be a potential source of germ transmission into humans. The fact of detecting DNA-MAP in 1/3 of all milk samples points to the likelihood of occurrence ofMAP bacilli in milk of animals not displaying seropositive or serodoubtful responses.
10

Paratuberkuloza bydła

59%
Lipiec M.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2015
|
tom 10
|
nr 2 Monografia
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