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Plants under attack of herbivores can emit increased amounts of volatile compounds from their leaves. Similarly, mechanically-injured plants can emit volatile chemicals that differ both quantitatively and qualitatively from undamaged plants. In this experiment, mechanical injury increased the release of the secondary metabolites linalool (3,7-dimethyl-1,6-octadien-3-ol) and linalool oxide (5-ethenyltetrahydro-2-furanmethanol) by wheat plants. The amounts released varied significantly with injury type and the period of time after injury. The time interval for the volatile collection within the photophase also influenced the amount collected for each day. The increased emission of these compounds, as a result of injury, may be explained as a defense mechanism against wounding. The role of these plant volatiles can be further investigated in the context of plant response to mechanical injury, within the broader context of all types of injury
The aim of this study was to examine whether and how different odorants placed on the bodies of female mice, but having no reward value for the males, affect courtship and mating behaviour of male mice towards females in oestrus and thus emitting female pheromones. In this manner, certain consequences of concurrent activation of the main olfactory system and the vomeronasal system were investigated. Four different odorants (white musk, lavender, peppermint and valerian) were used for swabbing female mice in oestrus. Using a total of 160 sexually naive outbred mice of both sexes, divided for each of 4 odorants into controls (not swabbed with odorant) and two experimental groups, in the experimental group I the females observed previously as controls were swabbed with one of the 4 odorants, while in the experimental group II, new naive females were swabbed with one of the 4 odorants. The females were observed in individual cages for 30 min. each, together with a respective sexually naive male. The latency between introduction of a male into a cage with a previously swabbed female and initiation of courtship and mating behaviours by males (sniffing, circling, misdirected mounting, copulation failures, successful copulation) was recorded. Latency to the occurrence of all sexual behaviours was significantly longer in experimental groups compared to controls. Latency to initiation of courtship behaviour, especially sniffing and circling, was shorter towards females swabbed with peppermint odour than for other odorants, indicating no aversion to this odour. However, the peppermint odour completely inhibited copulation. It is concluded that alien volatile odours with no reward value nevertheless exert differentiated suppressing effects on female mice pheromones inducing courtship and mating behaviour. Thus, it is hypothesized that the activation of the main olfactory system suppresses the accessory vomeronasal system.
A field experiment was carried out in two types of forest environment — a relatively homogenous mixed coniferous forest with low plant species diversity and a reach and spatially differentiated alder wood. Spatial variation in activity of voles was sampled by placing ink pads and paper inside PVC waterpipes laid in a line on the forest floor. In the first stage of the experiment, use of space by bank volesClethrionomys glareolus (Schreber, 1780) was analysed without any bait in track collection tubes. In the second stage, we used an attractant in the form of scent collected from alien bank vole individuals of different sex (males, females), age (juveniles, adults) and physiological state (sexually active and not active). In the coniferous forest the distribution of number of visits in tracking tubes was of aggregated character both before and after providing odour of alien individuals. In the alder wood the aggregation level that had been initially lower than in the coniferous forest increased after providing scent. As a consequence, placing scent of alien individuals resulted in decreasing differences in animal distribution in both habitats. The results allow also to the supposition that the reaction to scent depends on sex, age, and the physiological state of the donor.
Odour and odorants may affect the quality of life of exposed individuals. A review of the literature on olfaction and reactions to odours was carried out with the aim of reaching an understanding of their influence on well-being and health, and to suggest possible improvements in odour environment. This review has focussed specifically on the impact of animal production emissions. Factors like emission and air movements form the physical odour levels, and individual parameters involving psychological and social factors determine the human response. An odour may have positive as well as negative effects on well-being. Learning may be important for induced approach or avoidance behaviour. Common sites of irritation and injury from odorants are the respiratory organs and the nose. In most cases, the protection system triggered by the trigeminal nerve prevents severe effects. Increased frequencies of a number of respiratory and stress-related symptoms are found in the vicinity of animal production facilities. Explanations may be odour-mediated symptoms through annoyance and/or co-existing compounds like dust and gases with synergistic effects. Besides hydrogen sulphide, a number of gases related to animal production have hazardous properties and might be contributory elements despite their low concentrations. Important factors affecting mood, stress, and perceived health are odour levels, exposure time, sensitivity, unpleasantness, cognition and coping. Odour unpleasantness influences annoyance and might be interesting for regulatory purposes.
The paradigm regarding the ecology of populations of small rodents has had several basic theses: (1) the individual has a home range or is a migrant; (2) factors regulating population parameters (including density) include spatio-social relations between indi­viduals; and (3) the phenomena observed on trial plots with a system of live traps are the same as those beyond the plot. However, the home-range concept in small rodents is open to criticism in that: (1) observations point to much greater ranges of spatial activity in these animals than has been shown hitherto in plot-based studies with systems of live traps; (2) there is great variability in the directions and extent of the spatial activity of small rodents; (3) observations suggest that what is regarded as the area of spatial activity of an individual has limited information content since the "tenant" makes use of the area via tracks and trails along which it moves. Transfers of studies on odour-related information in small rodents from the laboratory to natural populations confirm the significance of odours in the life of these animals and in integrating their populations. It is possible that the need to leave information in the environment (informational conditioning) provokes considerable locomotory and spatial activity in small rodents. Live traps with bait (food) limit the spatial activity of animals. The set of traps in which an individual is caught thus represents not home range but trap range. A new paradigm for the ecology of small rodents should comprise the propositions that: (1) the function of olfactory information is the integration of a population into an ecological system; (2) the maintenance of odour-mediated condi­tioning in the environment is one of the reasons for locomotor and spatial activity; (3) the patchy distribution of food in the environment (including study plots with traps) gives rise to changes in the use made of space by individuals; (4) the phenomena observed on research plots with live traps containing bait are different to those ongoing at the same time outside these areas. Such a paradigm requires reinterpretation of research results obtained hitherto as well as the application of new research methods.
The present research assessed the treatment effi ciency of odorogenous pollutants in air from a hatchery hall vented on organic and organic-mineral beds of an enclosed-container biofi lter. In this study, the following media were used: organic medium containing compost and peat (OM); organic-mineral medium containing bentonite, compost and peat (BM); organic-mineral medium containing halloysite, compost and peat (HM). The concentration of odorogenous gaseous pollutants (sulfur compounds and amines) in the hatching room air and in the air after biotreatment were determined by gas chromatography. In the hatchery hall among the typical odorogenous pollutants, there were determined 2 amines: 2-butanamine and 2-pentanamine, hydrogen sulfi de, sulfur dioxide, carbon disulfi de, sulfi des and mercaptans. Ethyl mercaptan showed the highest levels as its mean concentration in the hatchery hall air exceeded 60 μg/m3 and in single samples even 800 μg/m3. A mean concentration of 2-butanamine and sulfur dioxide in the examined air also appeared to be relatively high – 21.405 μg/m3 and 15.279 μg/m3, respectively. In each fi lter material, the air treatment process ran in a different mode. As the comparison reveals, the mean reduction of odorogenous contaminants recorded in the hall and subjected to biotreatment was satisfying as it surpassed 60% for most established pollutants. These high removal values were confi rmed statistically only for single compounds. However, a low removal level was reported for hydrogen sulfi de and sulfur dioxide. No reduction was recorded in the bentonite supplemented medium (BM) for sulfur dioxide and methyl mercaptan. In the organic medium (OM) no concentration fall was noted for dipropyl sulfi de either. In all the media investigated, the highest removal rate (100%), not confi rmed statistically, was observed for carbon disulfi de. Very good results were obtained in the medium with a bentonite additive (BM) for both identifi ed amines, whose mean elimination rate exceeded 60% (p≤0.05). The present research proved that diethyl sulfi de is most susceptible to biofi ltration (over 80%) in the bed supplemented with halloysite (HM) and bentonite (BM) (p≤0.05).
Numerous authors indicate a significant role of chemical information (scent) left in the environment in communication between individuals in natural mammal populations. The basic functions of olfactory signals in small forest rodents are believed to include information related to reproduction: identification of sex, age, sexual status and social position of prospective sexual partners. It seems that scent-marking of movement routes thus creating a network of scent trails in the environment, channelling animal movements and making finding the way easier (particularly in case of long-distance movements) is another very important function of scent marking. Some examples of field experiments that confirmed the concept of scent trails are presented and discussed. However, some experiments suggest that besides the use of olfactory information animals have also other possibilities of finding the way in unknown space.
In this experiment, we tested the hypothesis that males of root voles (Microtus oeconomus Pallas) of different social ranks display different behavioural strategies. To document behavioural differences between social ranks, we investigated patterns in the behavioural responses to urine cues from familiar and novel individuals in a choice maze. Ten pairs of male voles were effectively used in this experiment. All behaviour was recorded with OBSERVER 5.0. When experiment was finished, video tapes were transformed into digital data. Then all data were analyzed by SPSS. The results showed that the approach latency of subordinates was shorter for familiar odours than novel ones, dominant individuals preferentially entered the strange odourant box, subordinates preferred familiar odours over novel ones, subordinates spent more time visiting familiar odours compared to the novel odours, dominants preferred novel odours to familiar ones, subordinates approached familiar odours more frequently than novel ones and self-groomed more often in the familiar odourant box than in the novel box, and dominant and subordinate individuals showed significantly different countermarking behaviours to familiar and novel odours. In conclusion, the dominants and subordinates displayed different behaviour patterns when faced to familiar and novel conspecific males’ urine cues. The data support our hypothesis that differences in social rank induce differences in behavioural patterns.
Several vole species use scent marked runways radiating from their burrows for foraging and dispersion. These marks are probably used for social communication. This 4-day laboratory study investigated the environmental and social causations of marking inside pre-existing corridors in male and female common volesMicrotus arvalis (Pallas, 1778). Firstly I tested the novelty and the reinforcement hypotheses in isolated voles, predicting respectively a habituation or a continuous increase in mark deposition. I then confronted with each other two same-sex voles for two days to investigate the differences between males and females in the pattern of marks inside three corridors, one of which runs along the common partition with the neighbour. I tested the self-advertisement and territorial-defence hypotheses, respectively predicting in the presence of a neighbour either a similar marking between the three corridors or a greater marking in the corridor close to the neighbour than in the two other corridors. The results showed no habituation in marking, even in a familiar environment, confirming the reinforcement hypothesis. After the addition of a neighbour, only the females left more marks in the corridor that ran alongside the common border than in the two other corridors. The territorial-defence hypothesis was thus confirmed in (territorial) females while the self-advertisement was supported in (non-territorial) males. Finally, I tested the competitive-ability hypothesis in females, stating that the abundance of scent marks of an individual before a social interaction can predict its degree of intolerance in a future social interaction. The results from female pairs physically interacting for four days support the hypothesis.
The present study aimed to evaluate microbiological pollution of air with microorganisms belonging to different taxonomic and physiological groups, and to examine whether the effect of the municipal landfill site in Krzyż changes at various study sites located: in so called "zero zone" (operating landfill), at different distances from the landfill and in Tarnów. Municipal landfill site for the city of Tarnów is located in north-eastern part of the city, in Krzyż district, on the ground gently-sloping towards South-East. The present microbiological studies showed wide differences in numbers of bacteria, ranging from 5 x 101 to 439 x 104 colony-forming units (e.f.u.) per 1 m1 of air. Abundance of bacteria was the highest in summer, i.e. in July, August and September (maximum of 7.44 x 101 e.f.u, in August), and at the sites No. 5 and 2 (maximum of 10.46 x 104 e.f.u. at site No. 5).
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