Wyniki wyszukiwania

1

Optimization of process parameters for maximum poly[-beta-]hydroxybutyrate [PHB] production by Bacillus thuringiensis IAM 12077

100%

Adwitiya P., Ashwini P., Avinash A. K., Badri R., Dadhe K., Ponnamma V., Shivakumar S.

Polish Journal of Microbiology

|

2009

|

tom 58

|

nr 2

149-154

The study aimed at screening and identifying a potential poly-β-hydroxybutyrate (PHB) accumulating Bacillus strain and optimization of media parameters for increased PHB production by the strain. A Gram-positive bacterium that accumulated PHB was isolated from local garden soil of Bangalore. Based on morphological and physiological properties, and nucleotide sequence (about 1.5 kb) of its 16S rDNA it was identified as Bacillus thuringiensis IAM 12077. PHB production was found to be comparable to most of the Bacillus sp. reported to date. PHB production by this strain was dependent on nutrient limitation. Cell dry weight and PHB accumulation increased significantly under biphasic growth condition (from nutrient broth to nitrogen-deficient medium) as compared with growth in nutrient broth alone (from 0.32 g/l to 2.76 g/l cell dry weight; 24% to 43.37% PHB accumulation; 0.2 g/l to 1.2 g/l PHB production), with maximum accumulation at 24 h in nitrogen-deficient medium. Time course study of growth and PHB production by this strain in the nitrogen deficient medium showed that PHB production was associated with the stationary phase of growth. All the tested media containing different carbon and nitrogen sources supported growth and PHB production. Ultraviolet spectrum of the extracted polymer showed a characteristic peak at 235 nm.

2

Nitrites as a source of nitrogen for Stichococcus bacillaris Nag.

100%

Przytocka-Jusiak M., Rzeczycka M., Blaszczyk M.

Oceanologia

|

1983

|

tom 17

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Effects of different soil water content on biological nitrogen fixation at soybean

100%

Coskan A., Oz H., Atilgan A.

Infrastruktura i Ekologia Terenów Wiejskich

|

2013

|

nr 1/III

Biological nitrogen fixation (BNF) is the process that provides organic nitrogenous compounds to the plants by using molecular nitrogen in atmosphere. Higher plants are not capable to use molecular nitrogen in atmosphere as a nitrogen source to generate essential proteins. Therefore plants either should be fertilized by adequate nitrogenous fertilizers or the microorganisms which are capable to produce nitrogenase should provide nitrogen to the plants by BNF. From among a number of factors affecting BNF, soil moisture content and ambient temperatures are considerably effective on the fixation rate. Therefore the global warming would be dramatically defective on BNF, thus effects of soil moisture as well as soil and ambient temperatures on BNF should evaluate prior rising temperature. A pot experiment was carried out to determine the effects of soil water contents on BNF. Four different soil water contents (%25, %50, %75 and %100 of water holding capacity) were adjusted either every 3 days or just after plants indicate wilting point. Non-inoculated pots were added to experiment as a control. The results revealed that BNF is affected by different level of soil water content. The mechanism of this effect would not be the direct effect of water, but the side effect of water on soil oxygen content; therefore, an aeration capability.

4

Nitrate reductase activity and protein content in leaves and roots of two doubled haploid barley lines depengis on seedling age and nitrogen source

100%

Skoczek H.

Acta Physiologiae Plantarum

|

1992

|

tom 14

|

nr 2

5

The assessment of influence of different nitrogen compounds and time on germination of Lupinus angustifolius seeds and chemical composition of final products

84%

Kasprowicz-Potocka M., Walachowska E., Zaworska A., Frankiewicz A.

Acta Societatis Botanicorum Poloniae

|

2013

|

tom 82

|

nr 3

The aim of the research was to determine changes in the chemical composition in lupin seeds during seed germination in a solution containing selected nitrogen compounds. Blue lupin seeds of the Neptun cultivar were germinated for 3 or 4 days in darkness, at 24°C in 1% solutions containing the following nitrogen compounds: urea, nitric acid, ammonium sulphate, methionine or yeast extract. The control consisted of seeds germinated in water. The presence of all nitrogen sources has resulted in a poorer growth of the sprouts as compared to seeds germinated in water. There was a significant increase in the true protein content as well as a significant decrease in the content of crude ash and alkaloids in the germinated seeds. The seeds germinated for 4 days had significantly higher total protein content and significantly lower content of nitrogen-free extract compounds and oligosaccharides as compared to the seeds germinated for 3 days. A significant influence of the nitrogen source on the concentration of some amino acids was found. Cystine was found to be absent in seeds germinated in the nitric acid solution, while there occurred a twofold increase in the cystine content and a fivefold increase in the methionine content in seeds germinated in the aqueous solution of methionine and an 50% increase in the cystine concentration was observed in seeds germinated in the yeast extract and ammonium sulphate solution. Methionine and cystine were the limiting amino acids in all the samples, accept of seeds germinated in the methionine solution were it was valine. Aqueous solutions of ammonium sulphate and yeast extract were found to have the most advantageous influence on the chemical composition of lupin germination products.

6

ROS metabolism in Arabidopsis leaves under ammonium supply

84%

Podgorska A., Gieczewska K., Gardestrom P., Rasmusson A., Szal B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 2

7

Can nitrogen nutrition cause stress in plants?

84%

Starck Z.

Acta Physiologiae Plantarum

|

2007

|

tom 29

|

nr 1 Suppl.

8

Enhancement of biomass and total carotenoid content of a UV-resistant strain of Dietzia maris in response to different carbon and nitrogen sources

84%

Bakhtiyari A. S., Etemadifar Z., Borhani M. S., Zamanian N.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2021

|

tom 102

|

nr 2

9

Ability of Pseudomonas sp. to synthesize aminopeptidases in the presence of various carbon and nitrogen sources

84%

Jankiewicz U., Bielawski W.

Acta Microbiologica Polonica

|

2002

|

tom 51

|

nr 3

10

Nitrate reductase activity of radish cotyledons as affected by phytohormones and different nitrogen sources

84%

Sood C. R., Chanda S. V., Singh Y. D.

Acta Physiologiae Plantarum

|

2000

|

tom 22

|

nr 4

11

Streptomyces nigellus as a biocontrol agent of tomato damping-off disease caused by Pythium ultimum

67%

Helmy S. M., Zeinat K., Mahmoud S. A., Moataza S., Nagwa M., Amany H.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

|

2010

|

tom 13

|

nr 4

12

Effect of organic phosphorus and nitrogen enrichment of mesotrophic lake water on dynamics and diversity of planktonic microbial communities - DNA and protein case studies [mesocosm experiments]

67%

Chrost R. J., Adamczewski T., Kalinowska K., Skowronska A.

Polish Journal of Microbiology

|

2009

|

tom 58

|

nr 2

163-180

Effects of mesotrophic lake water enrichment with organic phosphorus and nitrogen substrates (DNA and model protein, bovine serum albumin - BSA) on dynamics and diversity of natural microbial communities (bacteria, heterotrophic nanoflagellates, ciliates) were studied in mesocosm experiments. Simultaneous enrichment with DNA and BSA strongly increased the abundance and biomass of all studied groups of microorganisms and induced changes in their morphological and taxonomic structure. The increased participation of large heterotrophic nanoflagellates cells (larger than 10 μm) in their total numbers and shifts in taxonomic and trophic Structure of the ciliates, from algivorous to small bacterivorous, species were observed. Grazing caused changes in bacterial size distribution in all enriched mesocosms. Large (10-50 μm) filamentous bacteria significantly contributed to the total bacterial numbers and biomass. Pronounced increase in populations of (β- and γ-Proteobacteria was found in lake water enriched with organic P and N sources, whereas α-Pmteobacteria did not change markedly in the studied mesocosms. DNA additions stimulated the rates of bacterial secondary production. BSA shortened the rates of bacterial biomass turnover in lake water. Relatively high and constant (~ 30%) percentage contribution of active bacteria (MEM+) in two mesocosms enriched with DNA and DNA+BSA suggested the important role of nucleic acids as a source of phosphorus for bacterial growth, activity and production. Numerous and statistically significant correlations between bacteria and protists indicated the direct and selective predator-prey relationship.

13

Response of copper, lead and zinc mobility and bioavailability to sludge application on different soils

67%

Ngole V. M.

Polish Journal of Soil Science

|

2007

|

tom 40

|

nr 2

125-138

Sludge of two different ages (3 years old; Type 1 sludge, and 3 months old; Type 2 sludge) were applied to the following soil types: two luvisols, an arenosol and a vertisol at different rates to determine whether sludge application had any effect on the partitioning and consequently, bioavailability and mobility of Cu, Pb and Zn. A five- stage sequential extraction procedure was used to determine the concentration of metals in the different fractions of the soil after sampling and three months after sludge application. Type 1 sludge had higher values for Cu, Pb, and Zn (115.9, 295.5 and 400 mg kg⁻¹, respectively) than Type 2 sludge (110.8, 228.6 and 341 mg kg⁻¹ respectively). These values were significantly higher than values for the background concentrations of the heavy metals in the different soils. Pseudo-total concentration of Cu, Pb and Zn in the soils therefore increased with increase in sludge application rate. Mobility and bioavailability of Cu, Pb, and Zn were affected by interactions between sludge application rate and soil type with sludge age having no significant effect. Some precaution should be taken when the period between sludge application and planting is relatively short to minimize heavy metal mobility and bioavailability.

14

Effect of differentiated nitrogen fertilisation on the yield and quality of leaf lettuce

67%

Chohura P., Kolota E.

Folia Horticulturae

|

2011

|

tom 23

|

nr 1

15

Effects of nitrogen nutrition on storage protein polypeptides of yellow lupin cotyledons cultured in vitro

67%

Ratajczak W., Gwozdz E. A., Miadowicz M.

Acta Physiologiae Plantarum

|

1996

|

tom 18

|

nr 4

16

Effect of different carbon and nitrogen sources and vitamins on growth of Azospirillum spp. isolated from coniferous ectomycorrhizae and sporocarps of ectomycorrhizal fungi

67%

Rozycki H., Strzelczyk E., Raczkowska E., Li C. Y.

Acta Microbiologica Polonica

|

1992

|

tom 41

|

nr 3-4

17

Effects of selected heavy metals ions on Streptomyces in presence of various nitrogen sources

67%

Smylla A.

Archiwum Ochrony Środowiska

|

1995

|

nr 3-4

18

Cell enlargement in suspension culture of carrot [Daucus carota L.Cv.Karo]. Preliminary studies

67%

Golczyk H.

Acta Biologica Cracoviensia. Series Botanica

|

1998

|

tom 40

Cytological studies of Daucus carota L. (cv. Karo) in suspension culture were made. Cells growing on medium without ammonium were larger and more vacuolated than were cells grown on the standard medium. Their sizes (cell area) ranged widely from 200 µrm2 to 40000 µm2. They also showed a very low level of aggregation (1%) as compared to an 81% frequency of aggregates in the standard suspension. Suspension deficient in ammonium had a .small percentage of 2-9-nucleated cells. Such multlnucleate cells were not observed in suspension with both nitrate and ammonium used as nitrogen source.

19

Auxotrophy-stimulated sensitivity to quaternary ammonium salts and its relation to active transport in yeast

59%

Lachowicz T. M., Oblak E., Piatkowski J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

1992

|

tom 40

|

nr 3

20

Production and partial characterization of high molecular weight extracellular alpha-amylase from Thermoactinomyces vulgaris isolated from Egyptian soil

51%

Abou Dobara M., El-Sayed A., El-Fallal A., Omar N.

Polish Journal of Microbiology

|

2011

|

tom 60

|

nr 1

Optimizing production of α-amylase production by Thermoactinomyces vulgaris isolated from Egyptian soil was studied. The optimum incubation period, temperature and initial pH of medium for organism growth and enzyme yield were around 24 h, 55°C and 7.0, respectively. Maximum α-amylase activity was observed in a medium containing starch as carbon source. The other tested carbohydrates (cellulose, glucose, galactose, xylose, arabinose, lactose and maltose) inhibited the enzyme production. Adding tryptone as a nitrogen source exhibited a maximum activity of α-amylase. Bactopeptone and yeast extract gave also high activity comparing to the other nitrogen sources (NH₄Cl, NH₄NO₃, NaNO₃, KNO₃, CH₃CO2 NH₄). Electrophoresis profile of the produced two α-amylase isozymes indicated that the same pattern at about 135145 kDa under different conditions. The optimum pH and temperature of the enzyme activity were 8.0 and 60°C, respectively and enzyme was stable at 50°C over 6 hours. The enzyme was significantly inhibited by the addition of metal ions (Na⁺, Co²⁺ and Ca²⁺) whereas Cl⁻ seemed to act as activator. The enzyme was not affected by 0.1 mM EDTA while higher concentration (10 mM EDTA) totally inactivated the enzyme.

Ograniczanie wyników

Optimization of process parameters for maximum poly[-beta-]hydroxybutyrate [PHB] production by Bacillus thuringiensis IAM 12077

Nitrites as a source of nitrogen for Stichococcus bacillaris Nag.

Effects of different soil water content on biological nitrogen fixation at soybean

Nitrate reductase activity and protein content in leaves and roots of two doubled haploid barley lines depengis on seedling age and nitrogen source

The assessment of influence of different nitrogen compounds and time on germination of Lupinus angustifolius seeds and chemical composition of final products

ROS metabolism in Arabidopsis leaves under ammonium supply

Can nitrogen nutrition cause stress in plants?

Enhancement of biomass and total carotenoid content of a UV-resistant strain of Dietzia maris in response to different carbon and nitrogen sources

Ability of Pseudomonas sp. to synthesize aminopeptidases in the presence of various carbon and nitrogen sources

Nitrate reductase activity of radish cotyledons as affected by phytohormones and different nitrogen sources

Streptomyces nigellus as a biocontrol agent of tomato damping-off disease caused by Pythium ultimum

Effect of organic phosphorus and nitrogen enrichment of mesotrophic lake water on dynamics and diversity of planktonic microbial communities - DNA and protein case studies [mesocosm experiments]

Response of copper, lead and zinc mobility and bioavailability to sludge application on different soils

Effect of differentiated nitrogen fertilisation on the yield and quality of leaf lettuce

Effects of nitrogen nutrition on storage protein polypeptides of yellow lupin cotyledons cultured in vitro

Effect of different carbon and nitrogen sources and vitamins on growth of Azospirillum spp. isolated from coniferous ectomycorrhizae and sporocarps of ectomycorrhizal fungi

Effects of selected heavy metals ions on Streptomyces in presence of various nitrogen sources

Cell enlargement in suspension culture of carrot [Daucus carota L.Cv.Karo]. Preliminary studies

Auxotrophy-stimulated sensitivity to quaternary ammonium salts and its relation to active transport in yeast

Production and partial characterization of high molecular weight extracellular alpha-amylase from Thermoactinomyces vulgaris isolated from Egyptian soil