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Electrophysiological studies on the mechanisms determining the kinetics and pharmacological modulation of GABAergic synaptic transmission

100%

Mozrzymas J. W.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

Enhanced microdialysis of neuropeptides

100%

Pettersson A., Markides K., Bergquist J.

Acta Biochimica Polonica

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2001

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tom 48

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nr 4

An enhanced microdialysis method for neuropeptides is described and some preliminary results of this novel approach are presented. The enhancement is achieved by adding a vehicle (solid support) to the perfusion fluid in order to increase the diffusion coefficient across the membrane and efficiently transport the analytes towards the detector. The microdialysis samples are desalted and then analyzed on an electrospray ionization orthogonal time-of-flight mass spectrometer. The preliminary results show major increase in signal when comparing this new approach of microdialysis with ordinary microdialysis.

3

Cholinergic innervation of human mesenteric lymphatic vessels

86%

D'Andrea V., Bianchi E., Taurone S., Mignini F., Cavallotti C., Artico M.

Folia Morphologica

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2013

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tom 72

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nr 4

Background: The cholinergic neurotransmission within the human mesenteric lymphatic vessels has been poorly studied. Therefore, our aim is to analyse the cholinergic nerve fibres of lymphatic vessels using the traditional enzymatic techniques of staining, plus the biochemical modifications of acetylcholinesterase (AChE) activity. Materials and methods: Specimens obtained from human mesenteric lymphatic vessels were subjected to the following experimental procedures: 1) drawing, cutting and staining of tissues; 2) staining of total nerve fibres; 3) enzymatic staining of cholinergic nerve fibres; 4) homogenisation of tissues; 5) biochemical amount of proteins; 6) biochemical amount of AChE activity; 6) quantitative analysis of images; 7) statistical analysis of data. Results: The mesenteric lymphatic vessels show many AChE positive nerve fibres around their wall with an almost plexiform distribution. The incubation time was performed at 1 h (partial activity) and 6 h (total activity). Moreover, biochemical dosage of the same enzymatic activity confirms the results obtained with morphological methods. Conclusions: The homogenates of the studied tissues contain strong AChE activity. In our study, the lymphatic vessels appeared to contain few cholinergic nerve fibres. Therefore, it is expected that perivascular nerve stimulation stimulates cholinergic nerves innervating the mesenteric arteries to release the neurotransmitter AChE, which activates muscarinic or nicotinic receptors to modulate adrenergic neurotransmission. These results strongly suggest, that perivascular cholinergic nerves have little or no effect on the adrenergic nerve function in mesenteric arteries. The cholinergic nerves innervating mesenteric arteries do not mediate direct vascular responses. (Folia Morphol 2013; 72, 4: 322–327)

4

Involvement of the cholinergic system in the central histamine-induced reversal of critical haemorrhagic hypotension in rats

86%

Yalcin M., Savci V., Jochem J.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 2

133-137

Histamine, acting centrally as a neurotransmitter, evokes a reversal of haemorrhagic shock in rats due to the activation of the sympathetic and the renin-angiotensin systems as well as the release of arginine vasopressin and proopiomelanocortin-derived peptides. In the present study, we demonstrate influences of cholinergic receptor antagonists on the central histamine-induced resuscitating action. Experiments were carried out in male anaesthetised Wistar rats subjected to a haemorrhagic hypotension of 20-25 mmHg, resulting in the death of all control animals within 30 min. Histamine (100 nmol) administered intracerebroventricularly (icv) at 5 min of critical hypotension produced a long-lasting pressor effect with increases in heart rate and peripheral blood flows, and a 100% survival at 2 h. Mean arterial pressure and blood flow changes were almost completely blocked by nicotinic receptor antagonist mecamylamine (246.3 nmol; icv) and partially inhibited by muscarinic receptor blocker atropine sulphate (14.8 nmol; icv). Cholinergic receptor antagonists given alone in the control saline-treated groups did not affect cardiovascular parameters in the post-bleeding period. In conclusion, there are interactions between the histaminergic and cholinergic systems, with an involvement of both nicotinic and muscarinic receptors, in the central cardiovascular regulation in haemorrhagic hypotension in rats.

5

White communicating rami in human embryos at the end of the fifth week

86%

Bruska M., Lupicka J., Pytel A., Wozniak W.

Folia Morphologica

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2010

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tom 69

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nr 2

White communicating rami were traced in 8 human embryos of developmental stages 14 and 15 (aged 33 and 36 postovulatory days, respectively). In embryos at stage 14 the white communicating rami were found in the spinal nerves T1 to T9. In embryos at stage 15 the white communicating rami were present at the spinal cord levels T1 to L3. (Folia Morphol 2010; 69, 2: 75–77)

6

Molecular characterization and intracellular regulation of the human serotonin transporter in Caco-2 cells

86%

Iceta R., Mesonero J. E., Aramayona J. J., Alcalde A. I.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 1

The serotonin transporter (SERT) has shown itself to be an effective pharmacological target in the treatment of mood disorders and some kinds of gastrointestinal syndromes. Most of the molecular studies of SERT in humans have been carried out using heterologous models. In this work, we have investigated the human enterocyte-like Caco-2 cell line as a potential "in vitro" model to study the human SERT. The results show that these cells express a SERT mRNA identical to the human brain SERT, and a 70 kDa protein immunodetected using a specific antibody. The SERT activity levels in Caco-2 cells increased in correlation with the onset and maintenance of the morphological and functional differentiation of the cells. Caco-2 SERT was also shown to be a high affinity (Kt=0.216 µM) saturable, Na+-dependent transporter that was inhibited by fluoxetine (IC50=17.6 nM). In addition, SERT activity was inhibited by the intracellular modulators protein kinase C and cAMP, either after short or long-term treatment. In short, the expression and molecular characteristics of the human SERT in Caco-2 cells indicate that this cell line may be an ideal tool to study in vitro the physiology and pharmacology of human SERT.

7

PACAP in vertebrates: receptors and signal transduction mechanisms

86%

Nowak J. Z., Zawilska J. B.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

8

Accumulation of inositol phosphates in human lymphocytes after cholinergic stimulation

86%

Laskowska-Bozek H., Bany U., Stokarska G., Ryzewski J.

Acta Biochimica Polonica

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1993

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tom 40

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nr 1

9

Characterisation of the role of the 14-3-3 protein family in potato plants

86%

Wilczynski G., Kulma A., Markiewicz E., Szopa J.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

10

The role of GABA-ergic system in human mammary gland pathology

86%

Mazurkiewicz M., Kleinrok Z., Grzybowska-Szatkowska L.

Folia Histochemica et Cytobiologica. Supplement

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2001

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tom 39

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nr 1

11

Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

72%

Rycerz K., Krawczyk A., Jaworska-Adamu J., Krawczyk-Marc I.

Folia Histochemica et Cytobiologica

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2014

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tom 52

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nr 4

12

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No effect of 7-nitroindazole on the anticonvulsant action of vigabatrin and oxcarbazepine in pentylenetetrazole-induced seizures in mice

72%

Luszczki J. J., Szadkowski M., Zadrozniak A., Wojda E., Dudra-Jastrzebska M., Andres-Mach M.

Journal of Pre-Clinical and Clinical Research

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2008

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tom 02

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nr 2

13

New facts and the concept of physiological regulation of the dopaminergic system function and its disorders

72%

Krzymowski T., Stefanczyk-Krzymowska S.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 3

14

The cannabinoid system in brain reward and addiction

72%

Molleman A., Hasenoehrl R.

Acta Neurobiologiae Experimentalis

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2005

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tom 65

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nr 5

15

The place of tachykinin NK2 receptor antagonists in the treatment diarrhea-predominant irritable bowel syndrome

72%

Szymaszkiewicz A., Malkiewicz A., Storr M., Fichna J., Zielinska M.

Journal of Physiology and Pharmacology

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2019

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tom 70

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nr 1

16

A mathematical approach for assessing the transport of large neutral amino acids across the blood-brain barrier in man

72%

Dahl R. H., Berg R. M. G.

Acta Neurobiologiae Experimentalis

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2015

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tom 75

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nr 4

Changes in the large neutral amino acid (LNAA) transport across the blood-brain barrier (BBB) is thought to contribute to brain dysfunction in a number of clinical conditions, including phenylketonuria, acute liver failure, and sepsis. Here, we present a novel approach for estimating BBB permeability and the LNAA concentrations in brain extracellular fluid, by demonstrating that they can be mathematically derived on the basis of kinetic constants of the BBB available from the literature, if cerebral blood flow and the arterial and jugular venous LNAA concentrations are known. While it is well known that the permeability surface area product of the BBB to a LNAA from blood to brain (PS1) can be calculated from the arterial LNAA concentrations and kinetic constants of the BBB, we demonstrate that the permeability surface area product from brain to blood (PS2) can be calculated by deriving the substrate activity of the saturable transporter from the kinetic constants and arterial and jugular venous LNAA concentrations, and that the concentration of the LNAA in brain extracellular fluid can then be determined. This approach is methodically simple, and may be useful for assessing the transcerebral exchange kinetics of LNAAs in future human-experimental and clinical studies.

17

Increased expression of CART, nNOS, VIP, PACAP, SP and GAL in enteric neurons of the porcine stomach prepyloric region following hydrochloric acid infusion

72%

Calka J.

Folia Histochemica et Cytobiologica

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2019

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tom 57

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nr 4

18

The new data on the serotonin and FMRFamide localization in the nervous system of Opisthorchis felineus metacercaria

72%

Terenina N. B., Kreshchenko N. D., Mochalova N. V., Nefedova D., Voropaeva E. L., Movsesyan S. O., Demiaszkiewicz A., Yashin V. A., Kuchin A. V.

Acta Parasitologica

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2020

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tom 65

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nr 2

19

The effect of the intramuscular application of selected neuropeptides on the morphology of muscle

72%

Gendek-Kubiak H.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

20

Function of the 14-3-3 protein in yeast

72%

Van Hemert M. J., Van Heusden G. P. H., Steensma H. Y.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

Ograniczanie wyników

Electrophysiological studies on the mechanisms determining the kinetics and pharmacological modulation of GABAergic synaptic transmission

Enhanced microdialysis of neuropeptides