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1

Detection and molecular identification of Cryptosporidium species among children with malignancies

100%
Ibrahim H. S., Shehab A. Y., Allam A. F., Mohamed M. A., Farag H. F., Tolba M. M.
Acta Parasitologica
|
2021
|
tom 66
|
nr 2
2

Determination of Cryptosporidium spp. in Van cats by nested PCR

100%
Orunc Kilinc O., Yilmaz A. B., Goz Y., Ozkan C., Denizhan V.
Medycyna Weterynaryjna
|
2018
|
tom 74
|
nr 07
The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YüzüncüYıl University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens – a total of 100 Van cats were analyzed in the study. A simplified formol-ether concentration method was applied to all samples. The samples were then examined microscopically by a modified Ziehl-Neelsen staining method. As a result of the staining, Cryptosporidium spp. oocysts were identified in stool samples of 3 kittens in the microscopic examination. After that, PCR and nested-PCR were conducted with suitable primers. Nested PCR identified 5 kittens (5%) as positive. As a result, it was concluded that nested PCR was a superior diagnostic method for Cryptosporidium diagnosis compared with staining methods and that infected cats could be a health hazard for other cats and individuals, since Cryptosporidium spp. agents infect via the faecal-oral route. Therefore, we believe it is necessary to raise the awareness of people in contact with cats.
3

Performance estimation of nested PCR-based assays for direct detection of Listeria monocytogenes in artificially contaminated materials

100%
Fiedoruk K., Zaremba M. L.
Polish Journal of Environmental Studies
|
2010
|
tom 19
|
nr 2
293-299
Our study evaluated the sensitivity and repeatability of nested PCR-based assays for directly detecting L. monocytogenes in artificially contaminated human serum, pasteurized milk and physiological saline samples. The detection of the hlyA (267bp) and iap (371bp) gene fragments was compared. The logistic regression (logit model) was used to evaluate the probability of detection of L. monocytogenes at various contamination levels and to calculate the number of test repetitions required to reach necessary detection limits (e.g. 50%, 80%, 90%, and 95%). The reliable limit of detection for both genetic markers, ensuring ≥95% probability of detection, was established at 102 CFU/100μL.
4

Nested PCR and IFAT in the diagnosis of porcine proliferative enteritis

100%
Plawinska J., Klimczak Z., Jakubowski T., Klawe W., Binek M.
Bulletin of the Veterinary Institute in Puławy
|
2004
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tom 48
|
nr 3
5

Detection of Plasmodiophora brassicae Wor. by bioassay and nested PCR methods

100%
Staniaszek M., Robak J., Marczewski W.
Vegetable Crops Research Bulletin
|
2001
|
tom 54
|
nr 1
6

The improvement of monitoring methods of cattle infection with bovine leukemia virus (BLV)

84%
Rulka J., Kubis P., Buzala E., Kaminski S., Deren W.
Polish Journal of Veterinary Sciences
|
2003
|
tom 06
|
nr 3 Suppl.
7

Evaluation of RE and B1 genes as targets for detection of Toxoplasma gondii by nested PCR in blood samples of patients with ocular toxoplasmosis

84%
Esboei B. R., Kazemi B., Zarei M., Mohebali M., Valian H. K., Shojaee S., Zahedipour F., Fallahi S., Mousavi P., Mahmoudzadeh R., Salabati M.
Acta Parasitologica
|
2019
|
tom 64
|
nr 2
8
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Detection of Giardia intestinalis DNA in environmental water and soil samples collected from the Pomerania Province and the Warmia-Masuria Province, Poland using real-time PCR and nested–PCR

84%
Lass A., Szostakowska B.
Annals of Parasitology
|
2016
|
tom 62
|
nr Suppl.
9

The evaluation of methods for detection of Bacillus anthracis spores in artificially contaminated soil samples

84%
Bielawska-Drozd A., Niemcewicz M., Bartoszcze M.
Polish Journal of Environmental Studies
|
2008
|
tom 17
|
nr 1
5-10
The nested PCR has been used to evaluate the usefulness and efficiency of different Bacillus anthracis spore isolation methods in contaminated soil samples. The best results were obtained using two methods described by Beyer et al. [1] and Cheun et al. [9]. Outer and inner pairs of primers were designed from the protective antigen gene of plasmid pXO1 as well as from genes B and C of the capsule region of the plasmid pXO2. The influence of soil types on obtained results was also studied. The type of soil samples did not affect the nested PCR results. Furthermore, the sensitivity of nested PCR and PCR – ELISA was also examined.
10

Transovarial persistence of Babesia ovata DNA in a hard tick, Haemaphysalis longicornis, in a semi-artificial mouse skin membrane feeding system

84%
Umemiya-Shirafuji R., Hatta T., Okubo K., Sato M., Maeda H., Kume A., Yokoyama N., Igarashi I., Tsuji N., Fujisaki K., Inoue N., Suzuki H.
Acta Parasitologica
|
2017
|
tom 62
|
nr 4
11

A nested PCR for Toxoplasma gondii detection from different animal samples

67%
Vitale M., Curro V., La Giglia M., Vitale F., Gozdzik K., Caracappa S.
Wiadomości Parazytologiczne. Suplement
|
2008
|
tom 54
12

Detection of cytomegalovirus in infant cerebrospinal fluid by conventional PCR, nested PCR and PCR-Digene

67%
Kapusta M., Dzierzanowska D., Dunin-Wasowicz D., Milewska-Bobula B., Dobrzanska A., Wojda U., Swiatkowska E., Blaszczyk G.
Acta Microbiologica Polonica
|
2001
|
tom 50
|
nr 3-4
263-274
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