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The effect of herbal materials on the P-glycoprotein activity and function

100%

Bogacz A., Deka-Pawlik D., Bartkowiak-Wieczorek J., Karasiewicz M., Kujawski R., Kowalska A., Chalas A., Czerny B., Grzeskowiak E., Mrozikiewicz P. M.

Herba Polonica

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2013

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tom 59

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nr 4

P-glycoprotein (P-gp) encoded by the MDR1 (multidrug resistance 1) gene is ATP-dependent transporting protein which is localizated in the cell membrane. P-gp is expressed Review Article 130 A. Bogacz, D. Deka-Pawlik, J. Bartkowiak-Wieczorek, M. Karasiewicz, R. Kujawski, A. Kowalska, A. Chałas, B. Czerny, E. Grześkowiak, P. M. Mrozikiewicz mainly in organs with the secretory functions and its physiological role concerns tissue protection against xenobiotics. P-glycoprotein is involved in the permeability barriers of the blood-brain, blood-placenta directly protecting these organs. It participates in the transport of many drugs and other xenobiotics affecting their absorption, distribution, metabolism and excretion. The high P-gp activity in the cell membranes of cancer tissue is a major cause of lack of effectiveness of chemotherapy. Hence, the methods which could increase the sensibility of these pathological cells to cytostatics are still being searched. In the experimental studies it was shown that natural plant substances may have an effect on the expression level and activity of P-glycoprotein. Hypericum perforatum, Ginkgo biloba and Camellia sinensis increase P-gp activity while curcumin from Curcuma longa, piperine and silymarin inhibit this protein. Taking into account a wide substrate spectrum of P-gp, application of our knowledge on interactions of herbals and synthetic drugs should be considered in order to improve drug impact on different tissues.

2

Biofilm forming multi drug resistant Staphylococcus spp. among patients with conjunctivitis

86%

Murugan K., Usha M., Malathi P., Saleh Al-Sohaibani A., Chandrasekaran M.

Polish Journal of Microbiology

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2010

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tom 59

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nr 4

Biofilm forming multidrug resistant Staphylococcus spp. are major reservoirs for transmission of ophthalmic infections. They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. Our results indicate that they are genetically close to other community acquired MRSA.

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Studies on biosynthesis of silver nanoparticles using Rhizopus sp. and its antibacterial efficacy on E. coli MDR strains

86%

Hiremath J., Rathod V., Ninganagouda S., Singh D., Prema K.

International Letters of Natural Sciences

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2014

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tom 18

Nanotechnology is a field that is burgeoning day by day, making an impact in all spheres of human life. Biological methods of synthesis have paved way for the “greener synthesis” of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. In this context we have investigated extracellular biosynthesis of silver nanoparticles (AgNPs) using cell-free extract of Rhizopus spp.. Formation of AgNPs was indicated by the change in the colour of the cellfree extract from yellow to dark brown under static condition after 48 hrs of incubation. Characterization of AgNPs was carried out by UV-Vis Spectroscopy which gave sharp plasmon resonance peak at 429 nm corresponding to spherical shaped nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed AgNPs in the range of 25-50 nm. Scanning electron microscopy (SEM) showed the particles to be uniformly dispersed without agglomeration with smooth morphology. EDS showed the presence of elemental silver at 3kev. X-ray diffraction (XRD)-spectrum of the AgNPs exhibited 2θ¸ values corresponding to nanocrystal. These biosynthesized AgNPs were used to study their antimicrobial activity against Multi-drug resistant (MDR) E. coli strains, by Agar diffusion method. Zone of inhibition was measured. Synthesis of nanosized particles with antibacterial properties, which are called "nanoantibiotics", is of great interest in the development of new pharmaceutical products.

4

Prediction of the response to chemotherapy in ovarian cancers

86%

Surowiak P.

Folia Morphologica

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2006

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tom 65

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nr 4

285-294

Ovarian cancer represents the fifth most frequent cause of death as a result of malignant processes after cancers of the breast, large intestine, lung and stomach. Owing to the localisation of ovarian cancer, approximately 75% of cases are diagnosed at the III and IV stages of advancement according to FIGO. Because of the advanced stage of the disease surgery has to be followed by chemotherapy in most cases of ovarian cancer and therefore resistance to cytostatic drugs represents a major clinical problem. The potential to predict the response to therapy with the use of cytostatic drugs would enable the most effective drugs to be applied in individual cases, thus improving the efficiency of the treatment and restricting the development of resistance to cytostatic drugs. In the present paper the progress made so far in the prediction of the clinical course of ovarian cancer is reviewed. The significance of the expression of the ATP-binding cassette (ABC) transporters is described, including P-glycoprotein and MRP2, the principal representatives of the protein group. The importance of disturbed control of apoptosis and the overexpression of HER-2 and topoisomerase 1A are also discussed. Two sections are devoted to the most recent studies in the biology of ovarian cancer, pangenomic studies on gene expression using DNA microarrays and aberrations of DNA methylation.

5

Tigecycline susceptibility in multidrug resistant Acinetobacter isolates from Turkey

86%

Yilmaz F. F., Tasli H., Gul-Yurtsever S., Buyuk A., Hosgor-Limoncu M.

Polish Journal of Microbiology

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2013

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tom 62

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nr 3

The present study aimed to evaluate antimicrobial activity of tigecycline against 84 multidrug resistant (MDR) Acinetobacter spp. strains by disc diffusion and E-test methods. The results of disc diffusion test were compared according to two different interpretation ways. In addition, E-test results and the disc diffusion results that interpreted by both the methods were checked for compatibility. According to the disc diffusion test, 3 strains (3.57%) were found resistant to tigecycline when considering breakpoints suggested by Food and Drug Administration (FDA). On the other hand, none of the strains was found resistant to the evaluation criteria recommended by Jones et al. (2007). Considering E-test results of tigecycline, MIC₅₀ and MIC₉₀ values of tigecycline for Acinetobacter spp. were 0.75 and 1 mg/l, respectively. Based on FDA defined breakpoints for Enterobacteriaceae, any resistant isolate was detected. In conclusion, although there are some differences in the results, tigecycline was found quite effective on Acinetobacter spp. isolates with reference to the both disc diffusion and the E-test methods.

6

Novel therapies of multidrug-resistant Pseudomonas aeruginosa and Acinetobacter spp. infections: the state of the art

86%

Wroblewska M.

Archivum Immunologiae et Therapiae Experimentalis

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2006

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tom 54

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nr 2

7

Transport of organic anions by multidrug resistance-associated protein in the erythrocyte

86%

Rychlik B., Pulaski L., Sokal A., Soszynski M., Bartosz G.

Acta Biochimica Polonica

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2000

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tom 47

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nr 3

The active transport of oxidized glutathione and glutathione S-conjugates has been demonstrated for the first time in erythrocytes and this cell remained the main subject of research on the glutathione S-conjugate pump for years. Further studies identified the glutathione S-conjugate pump as multidrug resistance-associated protein (MRP). Even though cells overexpressing MRP and isolated MRP provide useful information on MRP structure and function, the erythrocyte remains an interesting model cell for studies of MRP1 in its natural environment, including the substrate specificity and ATPase activity of the protein.

8

Resistance and serotypes evolution of Streptococcus pneumoniae strains isolated in West Pomerania region of Poland in the years 2001-2003

86%

Nowosiad M., Giedrys-Kalemba S.

Advances in Agricultural Sciences

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2006

|

tom 10

9

There is no evidence for the existence of complex formation between doxorubicin and glutathione

86%

Marszalek M., Bartosz M., Bartosz G.

Cellular and Molecular Biology Letters

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2003

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tom 08

|

nr 2

Doxorubicin is co-transported with glutathione by several multidrug resistance proteins (MRPs). In order to check whether weak non-covalent aggregates between doxorubicin and glutathione can be formed, which might be substrates for the transporter, the effect of glutathione on the partition coefficient of doxorubicin was studied. No evidence of an effect of glutathione (at levels up to 20 mM) on the partition coefficient of doxorubicin was found in the pH range of 4.0-7.4. These results indicate that non-covalent doxorubicin-glutathione complexes do not form.

10

Comparative studies on cell stimulatory, permeabilizing and toxic effects induced in sensitive and multidrug resistant fungal strains by amphotericin B [AMB] and N-methyl-N-D-fructosyl amphotericin B methyl ester [MFAME]

86%

Szlinder-Richert J., Cybulska B., Grzybowska J., Borowski E., Prasad R.

Acta Biochimica Polonica

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2000

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tom 47

|

nr 1

N-Methyl-N-D-fructosyl-amphotericin B methyl ester (MFAME) is a new derivative of amphotericin B, which is characterised by low toxicity to mammalian cells and good solubility in water of its salts. The antifungal activity and effects of MFAME towards Candida albicans and Saccharomyces cerevisiae multidrug resistant MDR(+) and sensitive MDR(-) strains was compared with those of parent compound. The results obtained indicate that MDR(+) S. cerevisiae was sensitive to MFAME as well as to AMB. MFAME exhibited the same effects on fungal cells studied as parent antibiotic. The two antibiotics, depending on the dose applied induced cell stimulation, K+ efflux, and/or had a toxic effect.

11

Multidrug resistance reversal in cancer cells and in pathogenic microorganisms

86%

Michalak K., Hendrich A. B., Wesolowska O., Pola A., Lania-Pietrzak B.

Cellular and Molecular Biology Letters

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2002

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tom 07

|

nr Suppl.

12

Strategies for overcoming ABC-transporters-mediated multidrug resistance [MDR] of tumor cells

86%

Borowski E., Bontemps-Gracz M. M., Piwkowska A.

Acta Biochimica Polonica

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2005

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tom 52

|

nr 3

The development of multidrug resistance (MDR) of tumors is a major cause of failure in antitumor chemotherapy. This type of crossresistance is due to the expression of ABC transporter glycoproteins actively effluxing the drug from the cells against the concentration gradient at the expense of metabolic energy, thus preventing the accumulation in cells of therapeutic concentration of active agents. In this review strategies for overcoming this adverse phenomenon are discussed. They comprise the control of expression of MDR glycoprotein transporters and control of the functioning of the expressed transporter proteins. The latter approach is discussed in more detail, comprising the following general strategies: (i) development of compounds that are not substrates of efflux pump(s), (ii) use of agents that inactivate (inhibit) MDR proteins, (iii) design of cytostatics characterized by fast cellular uptake, surpassing their mediated efflux, (iv) use of compounds competing with the drug for the MDR protein-mediated efflux. Positive and negative aspects of these strategies are analysed, with special attention put on strategy based on the use of MDR modulators in combination therapy, allowing the restoration of cytotoxic activity of clinical cytostatics towards resistant tumor cells.

13

Association between existence of integrons and multi-drug resistance in Acinetobacter isolated from patients in Southern Iran

72%

Japoni S., Japoni A., Farshad S., Ali A. A., Jamalidoust M.

Polish Journal of Microbiology

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2011

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tom 60

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nr 2

Nosocomial infections caused by multi-drug resistant Acinetobacter pose a serious problem in many countries. This study aimed at determining the antibiotic susceptibility patterns and prevalence of different classes of integrons in isolated Acinetobacter. In addition, the association between production of specific bands in PCR assay and magnitude of multi-drug resistance was investigated. In total, 88 Acinetobacter strains were isolated from patients from October 2008 through September 2009. The Minimal inhibitory concentration (MIC) of 12 antibiotics conventionally used in clinics against the isolates, was determined by E-test method. The existence of integron classes was investigated by PCR assay through the amplification of integrase genes. The most effective antibiotic against Acinetobacter was colistin with 97.7% activity, followed by imipenem (77.3%) and meropenem (72.7%). The presence of integron classes 1 and 2 in 47 (53.4%) isolates was confirme, However, no class 3 was detected. The proportion of class 1, compared with class 2, was high (47.7% vs. 3.4%). The association between multi-drug resistance to norfloxacin, ceftazidime, gentamicin, ciprofloxacin, cefepime and amikacin and the presence of integrons was statistically significant. However, the association was not remarkable in many of the isolates which exhibited resistance to the rest of antibiotics. This may imply that in addition to integrons, other resistance determinants such as transposon and plasmid may also contribute to resistance. To reduce the pressure on sensitive isolates, comprehensive control measures should be implemented. Furthermore, wise application of effective antibiotics could help alleviate the situation. Colistin is the most effective antibiotic in vitro against Acinetobacter.

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Detection of resistance to anti-tuberculosis drugs in the clinical isolates of Mycobacterium tuberculosis from Slovakia through comparison between phenotypic and genetic methods and evaluation of resistance levels with clinical parameters

72%

Mokry J., Porvaznik I., Kusnir P., Dohal M., Solovic I.

Journal of Physiology and Pharmacology

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2019

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tom 70

|

nr 1

15

Occurrence and plasmid profiles of multidrug-resistant Enterobacteriaceae isolated from hawked soymilk samples in the Polytechnic of Ibadan Community, Nigeria

72%

Fadahunsi I. F., Babalola D. O.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2021

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tom 102

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nr 2

16

The in vitro antimalarial interaction of 9-hydroxycalabaxanthone and alpha-mangostin with mefloquine/artesunate

72%

Chaijaroenkul W., Na-Bangchang K.

Acta Parasitologica

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2015

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tom 60

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nr 1

17

Multidrug resistant Acinetobacter baumannii - the role of AdeABC [RND family] efflux pump in resistance to antibiotics

72%

Wieczorek P., Sacha P., Hauschild T., Zorawski M., Krawczyk M., Tryniszewska E.

Folia Histochemica et Cytobiologica

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2008

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tom 46

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nr 3

257-267

18

Influence of the multidrug transporter inhibitors on the activity of Kv1.3 voltage-gated potassium channels

72%

Teisseyre A., Duarte N., Ferreira M.-J. U., Michalak K.

Journal of Physiology and Pharmacology

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2009

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tom 60

|

nr 1

69-76

Using the whole-cell patch-clamp technique, the influence of selected multidrug resistance modulators, both plant-derived compounds and derivatives on the activity of voltage-gated potassium channels Kv1.3 was investigated. Twelve compounds with phenolic and terpenic structures were tested: the stilbenes piceatannol (1) and its tetramethoxy (2) and tetracetoxy (3) derivatives, the flavonoids naringenin (4) and its methylated derivatives: naringenin-4',7-dimethylether (5) and naringenin-7-methylether (6), and aromadendrin (7), the coumarins esculetin (8) and scopoletin (9) and ent-abietane diterpenes, helioscopinolide B (10) and its 3ß-acetoxy derivative (11) and helioscopinolide E (12). The studies were performed on a model system with Kv1.3 channels endogenously expressed in human T lymphocytes. Obtained data provide evidence that compounds 2, 5 and 6 applied at 30 µM inhibited the amplitude of recorded currents to 31%, 4% and 29% of its control value, respectively. On the other hand, compounds 3, 4, 7-12 (at 30 µM) and compound 1 (at 40 µM) did not affect significantly the channel activity. These results indicate that some methoxy-derivatives of the tested compounds are effective inhibitors of Kv1.3 channels. Since the inhibition of Kv1.3 channels may inhibit the proliferation of prostate, breast and colon cancer cells expressing these channels, the channel inhibitors may exert an antiproliferative action. This action combined with a simultaneous modulation of the multidrug resistance may be significant for a potential application of these compounds in cancer chemotherapy.

19

Doxorubicin-transferrin conjugate selectively overcomes multidrug resistance in leukaemia cells

72%

Lubgan D., Jozwiak Z., Grabenbauer G. G., Distel L. V. R.

Cellular and Molecular Biology Letters

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2009

|

tom 14

|

nr 1

113-127

Neoplastic cells frequently have an increased number of transferrin receptors. Coupling transferrin to an anti-neoplastic drug has the potential to overcome multidrug resistance (MDR). The purpose of this study was to examine the distribution and action of doxorubicin-transferrin conjugate (DOXTRF) in a leukaemia cell line (HL60), a multidrug-resistant leukaemia cell line (HL60ADR) and a normal tissue cell line (human fibroblasts). The intracellular accumulation of DOX and DOX-TRF was monitored by direct fluorescence. More DOX-TRF than free DOX was delivered to the tumour cells, and consecutively the levels of DNA double-strand breaks and apoptosis increased even in the multidrug-resistant cell line. In the normal tissue cell line, DOX-TRF did not accumulate, and therefore, the levels of DNA double-strand breaks and apoptosis did not increase. Cell viability was determined using the MTT assay. The IC50 for DOX-TRF was lower than the IC50 value for the free drug in both leukaemia cell lines. The IC50 values for the HL60 cells were 0.08 μM for DOX and 0.02 μM for DOX-TRF. The IC50 values for HL60ADR cells were 7 μM for DOX and 0.035 μM for DOX-TRF. In conclusion, DOX-TRF was able to overcome MDR in the leukaemia cell lines while having only a very limited effect on normal tissue cells.

20

Dissemination of class 1, 2 and 3 integrons among different multidrug resistant isolates of Acinetobacter baumannii in Tehran hospitals, Iran

72%

Taherikalani M., Maleki A., Sadeghifard N., Mohammadzadeh D., Soroush S., Asadollahi P., Asadollahi K., Emaneini M.

Polish Journal of Microbiology

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2011

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tom 60

|

nr 2

A total of 100 non-duplicate Acinetobacter baumannii isolates were collected from different hospitals in Tehran and were confirmed as A. baumannii by conventional biochemical and API testing. Antimicrobial susceptibility of these isolates was checked by a disk diffusion method in accordance with CLSI guidelines. The isolates were then detected as carrying class 1 and 2 integron gene cassettes by PCR evaluation and then genotyped by REP-PCR. More than 50% (n = 50) of the isolates were multidrug resistant. The results showed that more than 80% of all multidrug resistant A. baumannii strains carry a class 1 integron. Distribution of IntI 1 and IntI2 among A. baumannii isolates was 58% and 14%, respectively. Analysis of a conserved segment of class 1 integron showed a range from 100 bp to 2.5 kb. REP-PCR fingerprinting showed more than 20 genotypes among A. baumannii strains. There was no relationship between REP genotypes and the distribution of different classes of integrons. This is a comprehensive study on the distribution of different classes of integrons among A. baumannii in Iran. Considering the exact role of integrons in coding drug resistance in bacteria, the findings of this study could help us find antimicrobial resistant mechanisms among A. baumannii isolates in Iran.

Ograniczanie wyników

The effect of herbal materials on the P-glycoprotein activity and function

Biofilm forming multi drug resistant Staphylococcus spp. among patients with conjunctivitis

Studies on biosynthesis of silver nanoparticles using Rhizopus sp. and its antibacterial efficacy on E. coli MDR strains

Prediction of the response to chemotherapy in ovarian cancers

Tigecycline susceptibility in multidrug resistant Acinetobacter isolates from Turkey

Novel therapies of multidrug-resistant Pseudomonas aeruginosa and Acinetobacter spp. infections: the state of the art

Transport of organic anions by multidrug resistance-associated protein in the erythrocyte

Resistance and serotypes evolution of Streptococcus pneumoniae strains isolated in West Pomerania region of Poland in the years 2001-2003

There is no evidence for the existence of complex formation between doxorubicin and glutathione

Comparative studies on cell stimulatory, permeabilizing and toxic effects induced in sensitive and multidrug resistant fungal strains by amphotericin B [AMB] and N-methyl-N-D-fructosyl amphotericin B methyl ester [MFAME]

Multidrug resistance reversal in cancer cells and in pathogenic microorganisms

Strategies for overcoming ABC-transporters-mediated multidrug resistance [MDR] of tumor cells