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In vitro micropropagation of Acacia auriculiformis from selected juvenile sources

100%

Ismail H., Kumar S. M., Aziah M. Y., Hasnida N. H., Nor Aini A. S.

Dendrobiology

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2016

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tom 75

The effects of 6- Benzylaminopurine (BA), different basal medium, sucrose concentration and gelling agent were investigated for shoot induction and multiplication of Acacia auriculiformis. Nodal explants derived from 5-month-old seedlings yielded the highest shoot multiplication rate in Murashige and Skoog medium (MS) with 0.44 μM BA, 30 g/L sucrose and 2 g/L Gelrite. The highest mean number of shoots (10) and mean length of shoots (5.07mm) were also obtained in this medium. Qualitative observation of the shoots cultured in 0.44 μM BA were greener and vigorous in growth as compared to shoots cultured on higher concentrations of BA (22.2 μM). MS medium produced a significantly higher number of shoots (18) compared to Woody Plant Medium (WPM) (11) and B5 medium (10). Media solidified with different gelling agents also produced a significantly different number of shoots with 2 g/L Gelrite produced the highest number of shoots (23). The highest percentage of shoots rooted was found in the MS medium without any growth regulators (40.0%) followed by medium supplemented with Indole-3-butyric acid (IBA) at 9.84 μM and the combination of 9.84 μM IBA with 5.37 μM α-naphthalene acetic acid (NAA) (33.3%). MS medium without any plant growth regulators produced the highest mean root length (84.33mm), whereas medium supplemented with 9.84 μM IBA produced the highest mean number of roots per shoot (4.33). Out planting of in vitro rooted shoots in shredded coconut husk as the substrate gave the highest percentage of survival (90%) during acclimatization in the greenhouse.

2

High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara

88%

Thirugnanasampandan R., Mahendran G., Narmatha Bai V.

Acta Physiologiae Plantarum

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2010

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tom 32

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nr 2

A protocol for in vitro propagation of Isodon wightii (Bentham) H. Hara from nodal segments was developed. Multiple shoots were successfully established on half strength MS medium supplemented with 4.4 µM BA. Enhancement of shoot multiplication and elongation was achieved on half strength MS medium supplemented with 4.4 µM BA and 1.4 µM GA₃. The regenerated shoots were rooted successfully on half strength MS medium supplemented with 4.9 µM IBA. Acclimatization of in vitro rooted shoots was successful. The in vitro regenerated plants grew well in the greenhouse without any phenotypic changes.

3

Micropropagation of Calycanthus fertilis

88%

Kulpa D., Kubus M.

Dendrobiology

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2010

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tom 64

Calycanthus fertilis Walt. is a shrub belonging to the family Calycanthaceae, has great potential as ornamental. In the literature there are no reports on methodpropagation of this shrub in in vitro cultures. Therefore, the aim of this study was a development of the method micropropagation of Calycanthus fertilis Walter. Shoot explants of the size 1 cm, with an apex or node with lateral meristems were placed on the media with mineral composition according to MS and WPM supplemented with BAP (from 0.5 to 2.0 mg·dm–3) and TDZ (from 0.1 to 0.5 mg·dm–3). BAP turnedout to significantly increase initiation frequency whereas TDZ inhibitedthe formation of adventitious shoots andcausedexplant death. The highest percentage of initiated explants were found in shoot fragments placed on WPM medium supplemented with 1.0 mg·dm–3 BAP. Primary explants which initiatedgrowth were transferredon the proliferation media containing WPM macroand microelement, with the addition of different cytokinin: BAP (from 0.5 to 2.5 mg·dm–3), KIN (1.0 to 5.0 mg·dm–3) or TDZ (from 0.1 to 0.5 mg·dm–3). Calycanthus multiplication in vitro shouldbe conductedon WPM media with 1.0 mg·dm–3 BAP. Proliferatedshoots were placedon the WPM rooting medium supplemented with auxins: IBA, IAA or NAA at the concentration from 0.1 to 2.0 mg·dm–3. Maximum rooting was obtained on WPM medium supplemented with 0.5 mg·dm–3 IBA. To sum up, it shouldbe statedthat an efficient method of micropropagation of Calycanthus fertilis Walt. has been developed.

4

Micropropagation of Chamaedaphne calyculata (L.) Moench by direct shoot organogenesis

88%

Zrobek-Sokolnik A., Slipiko A., Kucewicz M., Milewicz M., Holdynski C.

Polish Journal of Natural Sciences

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2011

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tom 26

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nr 3

The objective of this experiment was to investigate the effect of various concentrations of sucrose, 6-(γ,γ-dimethylallylamino)-purine (2iP) and pH values of Lloyd’s and McCown’s medium (1981, WPM) on the induction of lateral shoot growth in Chamaedaphne calyculata (L.) Moench. The explants were 2–3 cm nodal sections without the apex, with preserved leaves, from plants grown in vitro. The highest regenerative capacity was observed in culture media without cytokinin, with 58 mM sucrose content and pH 5.0. The lowest capacity for shoot organogenesis was reported in media with pH 5.6 with a higher sucrose content (88 mM) and 25 μM of 2iP. 80% of rooted explants were successfully transferred to ex vitro conditions. The survival rate of plantlets reached around 60% after three months of greenhouse cultivation.

5

Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets

88%

Koc I., Akdemir H., Onay A., Ciftci Y. O.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

Genetic stability of plants during in vitro propagation and conservation is one of the important aspects of plant biotechnology. In the present study, micropropagated P. lentiscus L. shoot cultures, which are cultivated for the mastic resin, have been cold stored up to 12 months at 4°C in the dark for different durations (2, 4, 6, 8, 10 and 12 months) and genetic alterations in cold storage conditions were evaluated. Growth parameters such as proliferation rate, shoot numbers per explant, shoot lengths and shoot forming capacity were also calculated. Since the highest proliferation rate (100 %) was obtained in 6 month-stored shoot cultures without any severe influence of cold stress on proliferation ability, amplified fragment length polymorphism (AFLP) and inter-retrotransposon amplified polymorphism (IRAP) marker systems were used to determine genetic stability in the plantlets after this storage period. Totally, 702 scorable bands were produced by 10 AFLP primer pairs. Genetic similarity value of the non-stored (control) plant and coldstored clones ranged from 0.66 to 0.84 with a mean of 0.74. In the case of IRAP, 159 bands were produced by 8 IRAP primers. Genetic similarity value of the non-stored plant and cold-stored clones varied from 0.65 to 0.83 and the average genetic similarity value was determined as 0.72. The genetic similarity indices revealed that genetic variability was similar in both techniques. Our results showed that tissue culture and especially cold storage of P. lentiscus L. may result transposons activation, thus could cause genetic instability.

6

Micropropagation of St. John's wort (Hypericum perforatum L.)

88%

Pawelczak A., Bartosz R., Pelc M.

Annals of Warsaw Agricultural University. Horticulture (Landscape Architecture)

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2004

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tom 25

7

Conservation of the endangered medicinal plant Picrorhiza kurroa through in vitro multiple shoot regeneration

75%

Chaudhary V., Singh S., Sharma R., Singh A., Sharma N.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 3

8

In vitro culture and fructan production by Vernonia herbacea (Asteraceae)

75%

Trevisan F., Chu E. P., Gaspar M., Carvalho M. A. M.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

Vernonia herbacea is a native species from the Brazilian Cerrado that accumulates about 80 % of inulin-type fructans in the underground reserve organs, the rhizophores. This work aimed at establishing a protocol for in vitro culture of V. herbacea, using seeds (achenes) and leaf discs as explants. Following germination and seedling growth, stem nodes from 6-month-old in vitro germinated plants were isolated and incubated on culture medium free of growth regulators for plant propagation and rhizophore formation. Fructan content and composition were evaluated in leaves, stems, roots and rhizophores from plants grown in vitro and compared with those of greenhouse-grown plants, in order to evaluate inulin production in vitro. Fructan contents of aerial organs and roots from in vitro plants were higher, compared with greenhouse plants, while in rhizophores, the opposite was observed. High performance anion exchange chromatography/pulsed amperometric detection profiles revealed the presence of the inulin homologous series in the aerial organs exclusively for in vitro plants, while in roots and rhizophores, this series was detected in plants grown in both conditions. These results indicate a modification in the source/sink ratio, leading to changes in the distribution of carbohydrates in in vitro plants. The leaf disc cultures on medium supplemented with indole-3-butyric acid induced the formation of roots (0.24, 0.49 µM) and friable callus (2.46 µM), while 6-benzylaminopurine (from 1.1 through 4.43 µM) induced compact callus. However, no shoot formation was observed. The use of seeds allowed the establishment of a protocol for in vitro culture and provides a model system for a better understanding of fructan metabolism in V. herbacea.

9

Efficient in vitro propagation of Vaccinium vitis-idaea L. plants on the double phase medium

75%

Mazurek M., Siekierzynska A.

Electronic Journal of Polish Agricultural Universities. Series: Horticulture

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2018

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tom 21

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nr 4

10

Propagation of blue honeysuckle [Lonicera caerulea var.kamtschatica Pojark.] in in vitro culture

75%

Dziedzic E.

Journal of Fruit and Ornamental Plant Research

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2008

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tom 16

93-100

11

Application of in vitro stevia (Stevia rebaudiana Bertoni) cultures in obtaining steviol glycoside rich material

75%

Luwanska A., Perz A., Mankowska G., Wielgus K.

Herba Polonica

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2015

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tom 61

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nr 1

Stevia is a plant attracting attention due to its capability to synthesize a group of chemical compounds with sweet taste, i.e. steviol glycosides. Steviol glycosides are successfully applied as a natural sweetener, and some of them have also therapeutic properties. This paper presents available information on the use of stevia plant tissue cultures with the focus on their potential application in food industry. Detailed analysis was done concerning the research employing in vitro culture techniques and the use of them in biosynthesis of secondary metabolites of high importance for the food industry. Both established achievements and most recent publications on stevia were used for assessment of practical applications of the aforementioned techniques and prospects for their development.

12

Micropropagation of Harpagophytum procumbens [Burch.] DC. ex Meisn.: the effect of cytokinins on shoot multiplication

75%

Grabkowska R., Wysokinska H.

Herba Polonica

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2009

|

tom 55

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nr 3

244-250

The procedure of in vitro propagation of Harpagophytum procumbens using shoot tips was developed. Shoot tips were cultured on Schenk and Hildebrandt (SH) agar medium supplemented with 0.57 µM indole-3-acetic acid (IAA) in combination with various cytokinins: 6-benzylaminopurine (BAP), thidiazurone (TDZ), kinetin or zeatin at four concentrations (2, 4, 6 or 8 µM). The best shoot multiplication rate (11.2 shoots/explant for 5 weeks) was achieved in the presence of 6 µM TDZ. The shoots were small and their elongation on SH medium supplemented with gibberellic acid (GA3 ) was necessary. Shoots of H. procumbens were rooted on full-strength or half-strength Murashige and Skoog (MS) agar medium either with or without auxin. Plantlets were transferred into pots and maintained in the greenhouse. After 1 month, the overall survival of plants was 83% but it decreased to 27% after 6 months.

13

Green pod culture and rapid micropropagation of Dendrobium chrysanthum Wall. - A horticultural and medicinal orchid

75%

Kumar De K., Majumdar S., Sharma R., Sharma B.

Folia Horticulturae

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2006

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tom 18

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nr 1

14

Indirect shoot regeneration from leaf explants of Kalanchoe pinnata

75%

Andrzejewska K., Durska A., Kempa M., Kopec P., Sobusiak A., Stec N., Trzebny A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

15

Micropropagation in some plum cultivars

75%

Aier N. B., Sharma S. D.

Fruit Science Reports

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1990

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tom 17

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nr 2

16

Cryopreservation of embryogenic tissues: a review

75%

Kulus D., Zalewska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

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nr 1

17

The influence of the term of excision and the explant type on Fritillaria persica L. regeneration in vitro

75%

Marcinek B., Swistowska A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

|

nr 1

18

Rooting of a trumpet creeper (Campsis radicans (L.) Seem.) microshoots in presence of auxins

75%

Parzymies M., Dabski M., Pogorzelec M., Kozak D., Durlak W., Dudkiewicz M.

Acta Scientiarum Polonorum. Hortorum Cultus

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2014

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tom 13

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nr 5

A trumpet creeper (Campsis radicans) is an attractive vine propagated vegetatively through cuttings. So far, there is very little available data on propagation of this beautiful species in tissue culture. There was a research conducted in order to estimate the possibility to obtain rooted Campsis radicans plants that had been cultivated in tissue culture. The plant material were shoots obtained by multiplication on Murashige and Skoog [1962] (MS) medium which were put in fresh media supplemented with auxins: IAA (indoleacetic acid), IBA (indolebutyric acid) or NAA (naphthaleneacetic acid). The shoots were rooted in vitro or transplanted into soil (peat + perlite 1 : 1 w/v). It was noted that Campsis radicans is a very difficult plant to root in tissue culture. No rooting was obtained in vitro. Use of a stimulating passage with a hormone free medium or the ones containing IAA or IBA in concentrations of 2.5–5 mg·dm-3 and rooting shoots directly in soil allowed to obtain 100% of well rooted plants.

19

Growth in vitro cultures of strawberry (Fragaria x ananassa Duch.) depending on different photoperiods

75%

Litwinczuk W., Zubel A.

Folia Horticulturae

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2005

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tom 17

|

nr 2

20

Effect of cytokinin concentration and explant type on micropropagation of Impatiens x walleriana Hook

75%

Witomska M., Lukaszewska A.

Annals of Warsaw Agricultural University. Horticulture (Landscape Architecture)

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2003

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tom 24

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