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The aim of the research was to determine the influence of foliar nutrition with Pentakeep V as well as differentiated nitrogen fertilisation on the content of macro- (Ca, K, Mg, Na, P and S) and micronutrients (B, Cu, Fe, Mn and Zn) in spinach leaves. Pentakepp V is a fertilizer containing 5-aminolevulinic acid (5-ALA), which is the direct precursor of chlorophyll in plants. In 2006-2007, a pot experiment with spinach Spinacia oleracea L. cv. Spinaker F1 was carried out. The plants were cultivated in 60 × 40 × 20 cm containers placed in an open field under a shade-providing fabric. Containers were filled with loamy clay soil (35% of sand, 28% silt, 37% clay) with the organic matter content of 2.44% in 2006 and 2.52% in 2007. The experiment design included 2 sub-blocks: with and without foliar nutrition. The plants were sprayed twice with Pentakeep V fertilizer in a dose of 0.02% w/v (16 ml 100 dm–3 – 3000 dm3 per 1 ha). In each sub-block, soil fertilization with nitrogen was applied: 1 – control (without N fertilization), 2 – 25 mg N dm–3 of the soil (50% of N dose), 3 – 50 mg N dm–3 of the soil (100% of N dose). Nitrogen fertilization was applied in the form of ammonium nitrate prior to seed sowing. Among all of the determined nutrients, a significant interaction between foliar nutrition and soil application of nitrogen was observed in the case of Ca and Fe content in spinach leaves. Foliar application of Pentakeep V decreased the content of Ca in plants without N fertilization as well as increased the amount of this element in plants fertilized with full dose of nitrogen (100% of N dose). These observations were further verified by the changes of Ca content in soil after plant cultivation. Increased uptake of Ca from soil was observed for plants treated with Pentakeep V and fertilized with the full dose of nitrogen. A higher content of this element in soil was noted after cultivation of plants receiving only Pentakeep V (without N fertilization). Specific interaction of Pentakeep V on the increased content of Fe in spinach fertilized with 50% dose of N was observed. In comparison with the control, N fertilization in both doses (regardless of the foliar nutrition) led to the decrease of the plant content of Ca, Na and Fe as well as resulted in the increased concentration of K in spinach leaves. Plants fertilized with half-dose of N were characterized by lower content of Mn but plants treated with 100 % of nitrogen had higher concentration of this element in comparison to the control. Foliar application of Pentakeep V (considered independently of N fertilization) did not significantly influence the content of these nutrients in spinach leaves. Weather conditions throughout both years of cultivation had no effect on the interaction between foliar nutrition with Pentakeep V and N fertilization on the content of analyzed nutrients.
In pot experiments with lettuce, the effect of chelated and mineral forms of manganese on the fresh matter of plants and on the content of Mn, Cu, Zn and Fe in lettuce leaves were compared. Lettuce plants were grown in a peat substrate where the manganese content was differentiated in four levels: 10, 20, 30, and 60 mg Mn‧dm⁻³. Independent of the form of the applied manganese, no differences were found in fresh matter of lettuce heads at manganese levels of 10 to 30 mg Mn‧dm⁻³ substrate. When the plants were nourished with the mineral form of manganese, then in comparison with the chelated form of manganese, the content of manganese in lettuce leaves was higher. The differentiated fertilization with manganese exerted an effect on the nutritional status of plants with copper, zinc and iron. After the application of chelated manganese, there was more copper and iron in the lettuce, but a lesser amount of zinc than in case of manganous sulphate application. The increase of manganese content in the substrate caused an increase of this component in the plants.
A study on reduced tillage effect on the micronutrients (B, Cu, Fe, Mn, Mo, Zn) content in two horizons (0-10 cm and 10-20 cm) of the arable layer of sandy soil in the maize monoculture was carried out. Micronutrient supply of maize plants and yielding was also tested. An increase of soil acidity in the 0-10 cm horizon of soil under the reduced tillage was stated. It was accompanied by the increased micronutrient content in comparison with both the deeper, 10-20 cm, horizon under the same two systems and the respective contents in the topmost horizon (0-10 cm deep) under the conventional tillage. This exerted an influence on micronutrient content in plants. A significant increase in the content of Cu and Zn alongside a decrease in the amounts of B, Mn, Fe and Mo in vegetative organs and grain of maize were observed under no-plough tillage. Significantly depressed maize grain yields occurred in the objects under no-plough tillage suggests that the changes of micronutrients contents in soil and maize plants could be one of the reasons. The results obtained indicate the necessity of microelement fertilization of maize monoculture especially when the reduced tillage is applied.
Changes in the contents of some selected microbiogenous elements (Cu, Mn, Fe, Zn) in haulms and tubers, yield of tubers and content of nitrates in potatoes grown in soil contaminated with cadmium were evaluated in pot experiments. Under conditions of constant supply of N, P, and K, the natural cadmium reserve in soil (0.21 mg Cd·kg⁻¹) was adapted with supplements of Cd (NO₃)₂, to content of 0.4, 1, 2 and 4 mg Cd·kg⁻¹. Increasing Cd levels in soil showed an irregular effect on content of some microelements in potato plants of the Impala cv. As compared with all other variants, a statistically highly significant increase of Cu, Mn, Fe and Zn content in tubers was observed at the level of 1 mg Cd·kg⁻¹. In haulms, on the other hand, there was highly significant decrease in contents of Cu, Mn, Fe and Zn at the level of 2 mg Cd·kg⁻¹. As compared with tubers, a markedly higher content of all microbiogenous elements under study was found in the haulms. Content of Cd in potato haulms and tubers increased highly significantly with its increasing content in soil. Cadmium was accumulated above all in haulms where its content was several times higher than in tubers. The approved limits of Cd, Cu, Mn, Fe and Zn contents in potato tubers were not exceeded in any of our experimental variants. Increasing doses of cadmium did not influence the yield of tubers. Content of nitrates in tubers did not exceed the approved limit of 300 mg NO₃⁻·kg⁻¹ of fresh tubers in none of our experimental variants.
Despite its numerous advantages, garlic chives (Allium tuberosum Rottler ex Sprengel) remains a species that is little known of and therefore underestimated in Poland. Cultivation requirements of garlic chives in the climatic conditions of West Pomerania have long been studied at the Department of Horticulture, West Pomeranian University of Technology in Szczecin. An example of such research is the experiment, conducted in 2007-2009, whose aim was to determine the effect of blanching two-year old garlic chives plants on the yield of dry matter and the macro- and micronutrient content. Control plants were non-blanched, two-year old plants. Each year, on 10th April, the seeds were sown directly in a field, 5 seeds per point, 30 x 20 cm apart. The plants were blanched by being covered with low plastic tunnels coated with one or two layers of black nonwoven polypropylene fabric. Blanched leaves were collected 4 weeks after installing the tunnels. Field experiments were established and carried out at the Vegetable Research Station in Dołuje (near Szczecin), and chemical analyses were performed at the Regional Chemical and Agricultural Station in Szczecin. The highest content of phosphorus and total nitrogen was found in the leaves blanched under a double layer of nonwoven fabric, as compared to non-blanched plants. An opposite trend was observed for potassium and calcium. The highest content of these macronutrients was noticed in non-blanched leaves of garlic chives. No significant effect of blanching was found regarding the leaf content of magnesium and sulphur. The highest level of copper was reported in non-blanched leaves, and the leaves covered with a double layer of nonwoven fabric were the richest in iron. The lowest content of zinc was found in the leaves blanched under one layer and the lowest level of manganese in those blanched under two layers of black nonwoven fabric.
In pot experiments, in spring 2006 and 2007, a comparison was made between the chelated and the mineral forms of copper on the yielding of lettuce and on the content of Cu, Zn, Mn and Fe in lettuce leaves. Plants were grown in peat substrate supplemented with Cu doses of 5, 25, 50 and 75 mg∙dm⁻³. A greater mass of lettuce heads was obtained after the application of the mineral form of copper. In the range of doses: 5–50 mg Cu∙dm⁻³, the mean yields of lettuce did not differ. In the second year of studies, the application of copper chelates in the dose of 75 mg∙dm⁻³ caused a high reduction of yield. The mean copper content in lettuce, after the application of the chelated Cu form, was twice higher than after the use of the mineral Cu form. Iron content in lettuce after the application of copper chelates was higher than after the use of copper sulphate, while the contents of zinc and manganese were smaller.
The results of three-year investigations with cv. Galaxy F1 cabbage grown commercially in an important agricultural region of South Poland are presented. The effect of the rate of ammonium sulphate and UAN (solution of ammonium nitrate + urea), the method of application (placement and broadcast technique) and foliar fertilization (urea and Supervit K) on Cu, Mn, Zn, Fe, B and Mo concentrations in edible parts of cabbage were surveyed. Nitrogen fertilizer was applied at the rate of 120 kg N ha–1. With the placement fertilization method, fertilizer was applied in rows 10 cm deep and 10 cm away from each plant when seedlings were transplanted. Foliar sprayings started at the beginning of intensive leaf growth and continued during the growing season at two-week interval. The foliar nutrition with 2% urea was carried out 3 times and 1% Supervit K was applied once. The field experiment was carried out in 2005-2007 with cv. Galaxy F1 white cabbage on silty clay soil containing 0.91-1.02% organic carbon and soil acidity pHH2O 7.18-8.21 Micronutrient concentrations were below the lower range of the content reported for cabbage grown in non-contaminated areas. Ammonium sulphate significantly increased Mn and Fe concentrations in cabbage heads and decreased B and Mo content. However, the environmental factors considerably modified this tendency. The method of N application affected Mn content in cabbage in 2007 and Mo in 2006 and 2007. Slightly higher manganese and molybdenum concentrations for placement fertilization were noted. In 2005 and 2006, the placement fertilization at the rate of 75% N and supplemented foliar sprays increased Mo content in cabbage.
Excessive amounts of Ni alter the micronutrients status of plants. In turn, S not only plays a pivotal role in plant growth but is also involved in enhancing stress tolerance. The purpose of this study was to examine the effects of Ni and S on the micronutrients status in spring wheat. Three S-sulphate levels (2-standard, 6, and 9 mM) and four Ni treatments (0, 0.0004, 0.04, and 0.08 mM) in Hoagland’s nutrient solution were applied for 2 weeks. Ni excess at the standard S level generally reduced Mn, Mo, and Zn as well as increased Cl content in roots and shoots, reduced shoot B content without changes in the root content of this element, whilst Fe and Cu content rose in roots and decreased in shoots. The translocation of Fe and Cu from roots to shoots was repressed, but that of Mo was enhanced. The Mn and Zn translocation depended on Ni concentration, while that of B and Cl remained unaffected. Intensive S nutrition of Ni-exposed wheat, as a rule, elevated root and shoot Fe, B, Cl, Mn, and Zn content and increased root Cu content. Simultaneously, various changes in Fe, Cu, Mn, Mo, and Zn translocation were found. Our results imply that intensive S nutrition can effectively improve the micronutrient status in wheat hampered by Ni.
The studies referred to the content of microelements: iron, manganese, copper and zinc as well as ginkgoflavonoglycosides in the leaves of maidenhair tree Hipokrates var. sampled in four terms separately from one-year old and two-year old shoots. It was found that, irrespectively of the harvest term, leaves taken from 1-year old shoots in comparison with 2-year old shoots contained more biologically active compounds, but they accumulated less amounts of Fe, Mn and Cu. The Zn level in leaves taken both from 1-year and 2-year old shoots was similar.
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