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The paper presents clinical diagnostic approaches and therapeutic effects of a specific protocol for the treatment of dogs with cardiovascular dirofilariasis in the Belgrade City (Serbia) territory. The study involved 50 privately owned dogs of different breeds, gender, and age, all showing signs of cardio - respiratory disorders. In addition to a general physical examination, blood tests were done to detect microfilaria and adult forms, and X-ray, ECG, and echocardiography were performed as well. At the first examination, 34 out of 50 examined dogs were positive for microfilaria and adult forms. Because of a lack of drug used as „the golden standard" in dirofilariasis treatment, it involved a combination of doxycycline (10 mg/kg) and ivermectin (6 (µg/kg) supported with Advocate - Bayer spot-on. After six months, the first control was performed while continuing treatment with the aforesaid protocol, and the second control was performed after 12 months. Of the 34 treated dogs, all were negative for microfilaria, as early as after the first six months of the treatment (100%). One dog was positive for adult forms of the parasite after six and 12 months. In echocardiography and X-ray examination after 12 months, six dogs showed evident chronic changes. At controls conducted at sixth month and at one year, the implemented therapy was successful in 97.05% (33/34) of primarily infected dogs.
A total of 1,243 Ezza people living in 10 communities of Ebonyi State, eastern Nigeria were examined between July 2002-January 2003 for lymphatic filariasis. This is the first time a filariasis survey due to Wuchereria bancrofti has been carried out in this state. Of the 1,243 persons examined, 210 (16.9%) had W. bancrofti microfilariae. Infection varied significantly among communities and ages (p<0.05) but not sex-related (p>0.05). The Ezza people are predominantly farmers and professional hired labourers. There was a close association between microfilaria rate and microfilaria -density in various age groups (r = 0.812; p<0.01). Microfilaria density is an important measure in the epidemiology, treatment and control of human filarisis in this endemic foci. Clinical signs and symptoms of the disease include elephantiasis, hydrocoele, dermatitis and periodic fever. Clinical symptoms without microfilaraemia and microfilaraemia without clinical symptoms were also observed. Of 1,603 mosquitoes dissected, Anopheles gambiae, An. funestus and Culex quinquefasciatus showed infectivity rates of 6.3%, 5.1% and 6.0% respectively. The affected persons and other key informants are unaware of the cause of the disease and attributed it to witchcraft, violation of taboo, bad water and food. Intervention strategies to be integrated into the on-going Community-Directed Treatment with Ivermectin (CDTI) project are discussed.
We studied blood parasites in wild birds within Hitoy Cerere Biological Reserve and Barbilla National Park in Costa Rica during the rainy season of 2004. We examined blood samples from 248 and 114 birds, respectively. Protozoan parasites of the genus Haemoproteus as well as microfilariae were found. Prevalence of Haemoproteus in birds was 0.8% and 4.4% in Hitoy Cerere and Barbilla, respectively, and differences were significant. Prevalence of infection by microfilariae was 8.1% and 3.5%, respectively, however, differences were not significant. Based on morphological characteristics, we divided microfilariae into two groups and nine morphotypes. In Hitoy Cerere, there were microfilariae of all nine morphotypes whereas in Barbilla we only found two morphotypes.
Aspects of human infection with Onchocerca volvulus was investigated in 9 villages in Okigwe LGA of Imo State, Nigeria between January 2010 and December 2011. The objectives were to compare the prevalence of different manifestations of Onchocerciasis according to gender and age, with view to determine if there had been a change in prevalence of Onchocerciasis among residents of some communities in Okigwe Local Government Area of Imo State. A cross sectional survey method was adopted for the study, blood free skin snips were collected from randomly selected consenting adults aged 5-62 years. Palpation for mobile subcutaneous lumps and clinical manifestation were observed among 960 persons comprising 511 males and 449 females. The commonest lesions observed were poor vision 17 %, nodules 15 %, leopard skin 20 %, lizard skin 15 %, and hanging groin 3 %. Musculo-skeletal pain (MSP) was recorded as one of the major complaints by 30 % of the subjects. The result showed that Onchocerciasis clinical manifestations are still prevalent, however nodules prevalence reduced among the sampled population (69.8 %).
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