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Role of rpoS in the regulation of glyoxalase III in Escherichia coli

100%

Benov L., Sequeira F., Beema A. F.

Acta Biochimica Polonica

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2004

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tom 51

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nr 3

Methylglyoxal is an endogenous electrophile produced in Escherichia coli by the enzyme methylglyoxal synthase to limit the accumulation of phosphorylated sugars. In enteric bacteria methylglyoxal is detoxified by the glutathione-dependent glyoxalase I/II system, by glyoxalase III, and by aldehyde reductase and alcohol dehydrogenase. Here we demonstrate that glyoxalase III is a stationary-phase enzyme. Its activity reached a maximum at the entry into the stationary phase and remained high for at least 20 h. An rpoS- mutant displayed normal glyoxalase I and II activities but was unable to induce glyoxalase III in stationary phase. It thus appears that glyoxalase III is regulated by rpoS and might be important for survival of non-growing E. coli cultures.

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Influence of the microenvironment of thiol groups in low molecular mass thiols and protein on the reaction with methylglyoxal

86%

Acimovic J. M., Stanimirovic B. D., Todorovic N., Jovanovic V. B., Mandic L. M.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

3

Characterization of phosphate transporter(s) and understanding their role in Leishmania donovani parasite

86%

Sindhu K. J., Kureel A. K., Saini S., Kumari S., Verma P., Rai A. K.

Acta Parasitologica

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2018

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tom 63

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nr 1

4

MiR-30b is involved in methylglyoxal-induced epithelial-mesenchymal transition of peritoneal mesothelial cells in rats

72%

Liu H., Zhang N., Tian D.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 2

Epithelial-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMC) is a major contributor to the pathogenesis of peritoneal fibrosis. EMT is at least in part caused by repeated exposure to glucose degradation products (GDPs), such as methylglyoxal (MGO). MiRNA contributes greatly to the EMT of PMCs. In this study, we tried to profile whether differences exist between the peritoneal membrane (PM) miRNA expression seen in control rats and that seen in rats injected intraperitoneally with MGO. We assessed whether miR-30b has a possible role in MGO-induced EMT of PMCs in rats. Comparative miRNA expression array and real-time PCR analyses were conducted for the control group at the start of the experiment and for the MGO group after 1 and 2 weeks. During the second week, the MGO rats were treated with: a chemically modified antisense RNA oligonucleotide (ASO) complementary to the mature miR-30b (ASO group); an miR-30b mismatch control sequence (MIS group); or a citrate buffer (EMT group). Bioinformatic analyses indicated that the 3′ untranslated region (3′-UTR) of bone morphogenetic protein 7 (BMP7) mRNA did contain a putative binding site for miR-30b. We also tried to investigate whether miR-30b targeted BMP7 in vitro by transfection. Of the upregulated miRNAs, miR-30b expression demonstrated the greatest increase. The administration of miR-30b ASO for two weeks significantly reduced α-SMA excretion and upregulated E-cadherin and BMP-7 expression. Our in vitro study showed that miR-30b directly targeted and inhibited BMP7 by binding to its 3’-UTR. Our results revealed that miR-30b is involved in MGO-induced EMT of PMCs in rats.

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NADPH-dependent alkenal-one oxidoreductase (AOR) supported the growth and the acclimation to the high light in Arabidopsis thaliana

72%

Saito R., Ifuku K., Sato F., Sakamoto K., Ikeda K., Tamoi M., Yamamoto H., Sugimoto T., Yamauchi Y., Amako K., Shimakawa G., Makino A., Miyake C.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

6

The evaluation of quality of manuka honeys in comparison with Polish requirements

58%

Wilczynska A.

Towaroznawcze Problemy Jakości. Polskie Towarzystwo Towaroznawcze

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2013

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nr 4

7

Impaired gastric ulcer healing in diabetic mice: role of methylglyoxal

58%

Naito Y., Takagi T., Oya-Ito T., Okada H., Suzuki T., Hirata I., Hirai M., Uchiyama K., Handa O., Uchida K., Yoshikawa T.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 7

8

Metabolic cardio- and reno-protective effects of empagliflozin in a prediabetic rat model

58%

Huttl M., Markova I., Miklankova D., Oliyarnyk O., Trnovska J., Kucera J., Sedlacek R., Haluzik M., Malinska H.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 5

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