Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 45

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  method
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
The aim of research was the assessent of the hygienic behaviour of worker bees. The hygienic behaviour was assessed by means of two test types: the traditional needle test and a test proposed by the authors that consisted in measuring the removal rate of cardboard pieces. The experiments were conducted over three seasons and comprised a total number of 60 colonies. It was proved that the cardboard removal test may replace the needle test in the selection process of bees resistant to brood diseases, but primarily in those bees that have a good expression of hygienic behaviour. The best test results are to be expected in tests which last for 24 hours.
Polska spośród wszystkich krajów rozwiniętych wykazuje najwyższy wzrost umieralności na chorobę niedokrwienną serca (IHD) i choroby naczyń mózgowych. Jest to związane z rozpowszechnieniem czynników ryzyka IHD, a wśród nich podwyższonego poziomu cholesterolu w surowicy i nadwagi. Oba te czynniki zależą w istotnym stopniu od nieprawidłowego żywienia. Świadomość pilnej potrzeby podjęcia działań profilaktycznych spowodowała powołanie Narodowego Programu Profilaktyki Cholesterolowej, za którego realizację resort zdrowia uczynił odpowiedzialny Instytut Żywności i Żywienia. Na Program składają się następujące działania: 1. Popularyzacja na skalę społeczną wiedzy na temat szkodliwych następstw podwyższonego poziomu cholesterolu oraz roli racjonalnego żywienia w profilaktyce IHD. 2. Szkolenie przed- i podyplomowe lekarzy na powyższy temat. 3. Organizacyjne usprawnienie wczesnej diagnostyki podwyższonych stężeń cholesterolu. 4. Podjęcie wysiłków na rzecz nowoczesnej polityki wyżywienia w Polsce. 5. Udoskonalenie metod leczenia zaburzeń gospodarki lipidowej i ich upowszechnienie.
The purpose of this paper was to compare two methods of fractionation of reactive phosphorus forms (RP) in bottom sediments of polymictic water reservoirs located in rural areas. As a criterion for estimation, quantities RP extracted in analyzed fractions versus the general content of Al, Fe and Ca were used. The sediments were collected in springtime, from rural areas with different land use in the drainage basin (arable fields, uncultivated land, farmyards). The sediments were collected with a KC-Denmark core sampler for bottom sediments, which makes it possible to collect samples leaving the upper structure of 6 cm in thickness intact. After mineralization, each sample was analyzed for the general phosphorus content and a speciation analysis was performed by two methods. With the first method (M1), the RP bonded to Al (Al_RP), Fe (Fe_RP) and Ca (Ca_RP) was determined. The second method (M2) was applied to determine fractions of reactive phosphorus released from the sediments under reduced conditions, mainly from bonds with iron and manganese (BD_RP), adsorbed on aluminium oxides mainly AL2O3 and other surfaces (NaOH_RP) and apatite phosphorus and bonds with carbonates. In almost all the sediments, most RP was determined in the phosphorus fractions with Ca extracted with acid solution. For the fractions obtained via the M1 method, more of the general RP, as well as phosphorus bonded to calcium and iron was found than for fractions obtained with the M2 method, and the differences might be attributed to translocation of phosphorus from organic to inorganic compounds during fractioning. The fraction of reactive phosphorus determined with the M1 method is correlated more strongly with the general content of the metal in the sediments than reactive phosphorus fractions determined with the M2 method. The strong correlation between the content of iron and the fraction of BD_RP in mineral sediments shows that it can be an indicator of the sensitivity of sediment to changes in the redox potential.
Wildcats Felis silvestris Schreber, 1775 were captured for radio collaring as a part of a study of their spatial distribution and social organisation in southern Slovenia between 1999 and 2001. Double-door box traps, with a roof that bears easily break (bear permeable traps), have been used to capture individuals. The distances between traps were between 550 to 2200 m. They were set out on logging roads and narrow trails in the forest. Nine wildcats, one lynx Lynx lynx (Linnaeus, 1758) and one feral cat were caught as target species and 19 badgers Meles meles and one bear cub Ursus arctos as non-targets. The catching success was 1 wildcat/58 trap-days and seems to be in correlation with the lunar cycle. Overall, 7 males and 2 female wildcats were captured which might indicate sex biased trapping selection. Methodological improvements shortened the time of handling procedures. Improved field protocols as well as restraining and immobilisation procedures increased reliability and safety of drug administrations, decreased potential chances for injuries and reduced overall stress of captured animals.
Both activities on quality improvement of the environment and a willingness to understand these processes are conditional on possessing reliable information that can be obtained from analytics and environmental monitoring. At present, analytics and environmental monitoring are among the most dynamically developing branches of chemical analysis. The pursuit of getting the complex information on environmental quality leads to developing new methods and analytical techniques. Previous studies and own experience entitle to present the most important tendencies in the development of analytics and environmental monitoring. These trends can be classified into two basic groups: - development of new methodical procedures, - new achievements in construction of measuring instruments (instrumentation). This paper presents the most important developments in both trends observed in chemical analysis.
The flotation method elaborated for recovery of Toxocara and other geohelminth eggs from soil is described. Soil samples of about 500 ml volume are picked from 3-cm superficial layer of the ground. In the laboratory, 40 g of dry and sifted material is analysed according to following procedure: 1 h standing, 20 minutes shaking and 3 minutes centrifugation (1500 rpm) in 5% sodium hydroxide (NaOH), then centrifugation, like above, with H₂O for washing the sample and next with the saturated sodium nitrate (NaNO₃) for flotation the eggs. Specimen is prepared by placing a cover slip on the positive meniscus of the flotation liquid.
The aim of this study was the quantitive determination of cis-9, trans-11 isomer by two methods: gas chromatography (GC) and silver-ion high performance liquid chromatography (Ag+HPLC) in ewes milk fat. The study was carried out on 20 ewes of Wrzosówka and Żelazneńska sheep, which were mantained at the same environmental and nutritional conditions. The milk samples have been collected from ewes at the age of 3—1 years and in the 4th week of lactation. The chemical composition, as well as fat content, was similar in both studied breeds. The amount of C18 fatty acids connected with the major CLA isomer did not show statistical differences between breeds, besides of C18:3, which was higher (P < 0.03) for Wrzosówka. The activity of A9 desaturase, the enzyme responsible for endogenous synthesis, was also higher (P < 0.01) for Wrzosówka ewes. The content of cis-9, trans-11 C18:2 isomer in milk fat of studied ewes determinated by GC method did not differ with this obtained by Ag+HPLC. The difference has not been noted also within studied breeds. Obtained results suggest, that major CLA isomer in milk could be sensitively identified what allow to precisely determining its amount both by classic GC method and Ag+HPLC method.
The aim of the study was to develop RT-PCR and Nested-PCR methods for the detection and identification of the West Nile virus in wild birds. The method was based on West Nile Virus genome non-coding region 3'NCR. All the samples were examined with NRT-PCR and also with a commercial West Nile Virus Kit Prodesse™. There was no positive case of West Nile virus infection in the examined samples. All control samples were positive in both methods. The NRT-PCR method proved to be quick and reliable for the testing of wild birds' tissue samples for WNV.
Background. Polychlorinated biphenyls (PCBs) are persistent organic pollutants widespread in the environment. Their ability to accumulate in living organisms leads to food contamination, which is the main route of human exposure to PCBs. During analytical procedure of PCB residue determination, losses of these compounds may occur, which contribute to obtaining underestimated analytical results. Adequate analytical method of chlorobiphenyls determination should be applied to avoid the losses and obtain high recoveries and furthermore to enable accurate estimation of the risk of consuming contaminated food. Especially fish, due to the high bioaccumulation, may contain considerable amounts of these compounds. The aim of this study was to determine if the method of sample preparation influences the recovery of non-ortho (PCB 77, 81, 126, 169) and mono-ortho (PCB 105, 114, 156, 157) PCB congeners in selected fish species. Materials and Methods. To prepare samples for chromatographic determination (HP 6890/5973 GC MS) two methods were applied. Fish muscle tissues were dried by rubbing in a mortar with anhydrous sodium sulphate or freeze dried (lyophilised) (LyoLAB 3000). The samples were fortified with a known amount of internal standard (decachlorobiphenyl), and some were additionally fortified with the standard solution of analysed PCB congeners. Results. nternal standard recoveries ranged from 57.61 ± 1.21% to 88.76 ± 4.03% in freeze-dried samples, and from 63.81 ± 5.11% to 97.50 ± 6.14% in samples rubbed with anhydrous sodium sulphate. Following lyophilisation, recoveries of analysed PCB congeners varied from 68.88 ± 11.74% for PCB 157 to 79.18 ± 12.33% for PCB 114. In the samples rubbed with anhydrous sodium sulphate the lowest recovery was determined for PCB 77 (72.40 ± 12.34%), the highest being typical for PCB 156 (83.47 ± 12.86%). Following lyophilisation, toxic equivalents (TEQs) for the examined fish species ranged from 0.0050 ng-TEQ·g-1 dry weight in salmon to 0.0299 ng-TEQ·g-1 dry weight in mackerel. Following rubbing with anhydrous sodium sulphate the highest toxic equivalent (0.0326 ng-TEQ·g-1 dry weight) was calculated for mackerel, and the lowest for salmon (0.0055 ng-TEQ·g-1 dry weight). Conclusion. The research has demonstrated that in most cases the results obtained with both methods have not differed significantly (P < 0.05), although freeze drying resulted in slightly higher losses of PCB congeners. Despite of smaller recoveries, freeze drying can be applied because of solvent saving and easier sample preparation.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 3 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.