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Food quality control by hyphenated separation techniques

100%

Walczak J., Pomastowski P., Buszewski B.

Health Problems of Civilization

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2015

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tom 09

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nr 1

Food as complex mixture of proteins, lipids, vitamins, etc. cannot be separated and identified by using in only one method. This article presents a revision on the hyphenated chromatographic techniques and methods used in food analysis and described main application in food science research, and determination of xenobiotics and their metabolites in environmental. Also article discusses applications of “omics” in food analysis (proteomics, transcriptomics, genomics, metabolomis) and new discipline of – foodomics.

2

Optimization of SPME-GC-MS analysis of chlorophenoxy herbicides

100%

Krzyzanowski R., Leszczynski B.

Herba Polonica

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2004

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tom 50

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nr 3-4

Application of solid phase microextraction (SPMŁi) and gas chromatography combined with mass spectrometry (GC-MS) for analysis of the chlorophenoxy herbicide residues was studied. The optimal conditions for SPME/GC-MS analysis of chlorophenoxy herbicides were determined as follows: adsorption time 15-20 min at 50°C and desorption time 6 min at 220°C. The highest concentration of MCPA was found in the tissues of the weeds of M. inodora, C. bursa pastoris, while the lowest in A. githago, P. aviculare. In addition to that, in May and June the studied herbicides were found in low quantities in the surface waters of the river of Liwiec.

3

Nanospray mass spectrometry for identification of peptides. Application of a novel interface

100%

Smoluch M. T., Sallberg M., Silberring J.

Acta Biochimica Polonica

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1999

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tom 46

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nr 2

Electrospray ionization mass spectrometry is a powerful tool for identification of biomolecules such as peptides, proteins, oligosaccharides and neurotransmitters. Recent development of the nanospray techniques, applied at ultralow flow-rates, allowed a sensitive analysis of compounds at femto/attomolar level. Here, we present application of a novel nanospray device for the analysis and fragmentation of peptides with high sensitivity on a sector instrument. The lowest applied flow-rate of the mobile phase was maintained at 50 nl/min with a sample load of 90 fmol. Nanospray also provided a complete analysis of 500 nl of the sample for over 10 min, including sequencing of as little as 40 pmol of a substance. Such analysis provides full structural information necessary to identify the molecules.

4

Determination of nystatin in honey by liquid chromatography coupled with mass spectrometry

86%

Cepurnieks G., Bartkevics V., Zeile Z.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

5

Mass spectrometry approaches in proteomic and metabolomic studies

86%

Rodziewicz P., Swarcewicz B., Chmielewska K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2014

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tom 95

|

nr 3

6

Analysis of the ability to form 2-phenylethyl alcohol by Galactomyces geotrichum MK017

86%

Grygier M., Majcher M., Myszka K.

Żywność Nauka Technologia Jakość

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2015

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tom 22

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nr 3

The aim of the study was to evaluate the ability of Galactomyces geotrichum MK017 for the biosynthesis of 2-phenylethyl alcohol and optimization of the culture medium composition in order to increase the yield of the product. The culture of Galactomyces geotrichum MK017 strain was carried out in the laboratory scale. For isolation of volatiles Solid Phase Microextraction (SPME) has been used. The identification and quantification of aroma compounds produced by examined microflora was determined by gas chromatography and mass spectrometry (GC/MS) system. The results showed that the tested mould Galactomyces geotrichum MK017 shows the potential for the production of 2-phenylethyl alcohol, but also for the synthesis of phenylacetaldehyde, phenylacetic and phenyllactic acids. For the optimization of the 2-phenylethyl alcohol production yield four different medium composition have been tested. The bioprocess of aroma compound production by tested microorganism was the most efficient on the medium composed of sucrose (80 g/l) and L-phenylalanine (21 g/l) and pH value of 5.0. Using this composition in a batch culture of 770 ml volume the highest concentration of 2-phenylethyl alcohol has been obtained – 6 mg/l. At the same time amount of phenylacetaldehyde, phenylacetic acid and phenyllactic acidhas reached 2.2 mg/l, 10.66 mg/l and 32.3 mg/l respectively.

7

Hemolytic activity of fractions of the ethanolic extract from faba bean cotyledons

86%

Karamac M., Kosinska A., Shimoyamada M., Amarowicz R.

Polish Journal of Natural Sciences. Supplement

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2006

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tom 03

79-85

8

Analytical pocedure for the determination of lincomycin in honey by liquid chromatography-mass spectrometry

86%

Bladek T., Gajda A., Gbylik M., Posyniak A., Zmudzki J.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

|

nr 2

205-209

A reliable and sensitive liquid chromatography-electrospray ionisation tandem mass spectrometry analytical method has been developed for the determination of lincomycin in honey samples. After extraction with phosphate buffer by ultrasound, the extracted solution was subjected to the polymeric solid-phase extraction cartridge to remove endogenous compounds. The analysis was carried out on a triple-quadropule tandem mass spectrometer in the multiple reaction monitoring (MRM) mode via electrospray interface operated in positive ionisation modes. The procedure was validated in accordance with the European Commission Decision 2002/657/EC. The mean recovery of the analyte was 80%, with the corresponding intra- and inter-day variation less than 10% and 15%, respectively. Decision limits (CCα) and detection capability (CCß) were 5.60 and 6.11 µg kg⁻¹, respectively.

9

Application of mass spectrometry [MS] in the structural analysis od selected polypeptides of boar seminal plasma obtained after two-dimensional electrophoresis [2-D PAGE]

86%

Kordan W., Malinowska A., Mogielnicka M., Lecewicz M.

Animal Science Papers and Reports

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2009

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tom 27

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nr 1

41-50

Two-dimensional electrophoresis (2-D PAGE) led to identification in the polypeptide maps of boar seminal plasma of four conserved polypeptides with identical molecular weight of 24 kDA, and different ranges of isoelectric point (pI): (1) 7.4-7.7, (2) 8.1-8.4, (3) 8.5-8.8 and (4) 9.2-9.4. In the current study the molecular structures of these polypeptides were analysed, for the first time, by mass spectrometry (LC – MS/MS). Computerized mass spectrometry analysis of the peptides obtained after trypsin-digestion of the polypeptides demonstrated their similarity to the family of spermadhesins (crystal structure of two members of the spermadhesin family), especially to epididymal spermadhesin AWN-1. In addition, homology was found of peptides 3 and 4 with a lysozyme C precursor (1,4-beta-N-acetylmuramidase C). The results presented might indicate the participation of the analysed polypeptides in the processes accompanying fertilization.

10

Analysis of water-soluble proteins by two-dimensional electrophoresis in the encystment process of Colpoda cucullus Nag-1 and cytoskeletal dynamics

86%

Sogame Y., Kojima K., Takeshita T., Kikuchi S., Shimada Y., Nakamura R., Arikawa M., Miyata S., Kinoshita E., Suizu F., Matsuoka T.

Acta Protozoologica

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2020

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tom 59

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nr 3-4

11

Determination of chloramphenicol residues in milk by gas and liquid chromatography mass spectrometry methods

86%

Sniegocki T., Posyniak A., Zmudzki J.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 1

Analytical methods based on gas chromatography- mass spectrometry (GC-MS/MS) and liquid chromatography- mass spectrometry (LC-MS/MS) were developed for the determination of chloramphenicol (CAP) in milk. GC-MS/MS was performed in negative chemical ionization (NCI) mode by monitoring the transitions - m/z 466→304, 466→322, and the results were compared with LC-MS/MS, in electrospray mode by monitoring the transitions of all the selected ions m/z 321→152, 321→257. The decision limit (CCα) for CAP determination by LC-MS/MS was established at a level of 0.11 µg/kg, while the corresponding value for GC-MS/MS was 0.083 µg/kg. Detection capability (CCß) for CAP by LC- MS/MS was 0.15µg/kg and for GC-MS/MS was 0.14 µg/kg. As it was found, both methods are useful for CAP determination in milk.

12

Detection of microbial contamination of packaging for foodstuffs by using muramic acid as a biomarker

86%

Mielniczuk Z., Pogorzelska Z.

Opakowanie. Special Edition

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2004

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nr 1

13

Current techniques in protein glycosylation analysis. A guide to their application

86%

Merry T.

Acta Biochimica Polonica

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1999

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tom 46

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nr 2

The importance of glycosylation in biological events and the role it plays in glycoprotein function and structure is an area in which there is growing interest. In order to understand how glycosylation affects the shape or function of a protein it is however important to have suitable techniques available to obtain structural information on the oligosaccharides attached to the protein. For many years the complexity of the structures required sophisticated analytical techniques only available to a few specialist laboratories. In many cases these techniques were not available or required a large amount of material and therefore the number of glycoproteins which were fully characterised were relatively few. In recent years there have been substantial developments in the analysis of glycosylation which has significantly changed the capability to fully characterise molecules of biological interest. A number of different techniques are available which differ in terms of their complexity, the amount of information which is available from them, the skill needed to perform them and their cost. It is now possible for many laboratories who do not specialise in glycosylation analysis to obtain some information although this may be incomplete. These developments do, however, also make complete characterisation of a glycoprotein a much less daunting task and in many cases this can be performed more easily and with less starting material than was previously required. In this review a summary will be given of current techniques and their suitability for different types of analysis will be considered.

14

Mass spectrometric analysis of head-to-tail connected cyclic peptides

86%

Macht M., Pelzing M., Palloch P., Sauerland V., Volz J.

Acta Biochimica Polonica

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2001

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tom 48

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nr 4

Tandem mass spectrometry is an extremly useful tool for high sensitive sequence identification of peptides. In the case of cyclic peptides fragmentation can easily be performed for sequence analysis. However, analysis is usually tedious due to the lack of a defined beginning and end of the sequence. Since cyclic peptides are a highly interesting class of compounds especially for the pharmaceutical industry, ways have to be found to identify their structures. In this work we demonstrate how software and dedicated analytical strategies can be used for detailed analysis of these substances.

15

Fast identification of trace-level pesticide residues in agricultural crops applying low-pressure gas chromatography mass spectrometry

86%

Walorczyk S.

Journal of Plant Protection Research

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2004

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tom 44

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nr 2

A method for the fast identification of trace levels of pesticide residues in agricultural crops was developed using low pressure gas chromatography/mass spectrometry (LP-GC/MS). The finalchromatographic determination took 12 minutes per sample while conventional GC/MS required at least 30 minutes. Also, improved peak shapes for dichlorvos, dimethoate, chlorothalonil, pirymethanil, pirimicarb, carbaryl, myclobutanil, flusilazole tebuconazole, fenarimol and iprodione were obtained which generally enabled lower limits of detection. The method was successfully applied to analysis of more than 40 pesticides in 120 samples of fruits, vegetables and cereals. With the aid of LP-GC/MS the number of samples analysed on the particular instrument could be at least doubled.

16

Detection and sequencing of new cyclic peptides from linseed by electrospray ionization mass spectrometry

86%

Stefanowicz P.

Acta Biochimica Polonica

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2001

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tom 48

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nr 4

Extract of Linum usitatissimum seeds was analyzed by ESI-MS and ESI-MS/MS. The analysis confirms the presence of previously reported cyclolinopeptides: CLA (c(Pro- Pro-Phe-Phe-Leu-Ile-Ile-Leu-Val) and CLB (c(Pro-Pro-Phe-Phe-Val-Ile-Met-Ile-Leu)). Cyclolinopeptides CLD and CLE, which contain methionine oxide, were detected in small quantities only. These peptides likely result from the oxidation of their precursors, not reported previously: CLD' (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Leu)) and CLE'(c(Pro-Leu-Phe-Ile-Met-Leu-Val-Phe)), present at higher concentrations in unoxi- dized extract. Two new cyclic octapeptides: CLF (c(Pro-Phe-Phe-Trp-Val-Met-Leu- Met)) and CLG (c(Pro-Phe-Phe-Trp-Ile-Met-Leu-Met)) were detected and their sequences were proposed on the basis of CID experiments and similarity with those of CLD.

17

Application of mass spectrometry to structural identification of flavonoid monoglycosides isolated from shoot of lupin [Lupinus luteus L.]

86%

Franski R., Bednarek P., Siatkowska D., Wojtaszek P., Stobiecki M.

Acta Biochimica Polonica

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1999

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tom 46

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nr 2

Flavonoid glycosides constitute important group of plant secondary metabolites. This class of natural products play significant role in different physiological processes. A new methodological approach where mass spectrometric techniques are applied to structural studies of this class of compounds is presented. Four flavonoid O-monoglycosides and one C-monoglycoside were isolated from green parts of lupin (Lupinus luteus L.). Several different mass spectrometric techniques were applied to structural elucidation of isolated compounds. Desorption ionization mass spectrometry was used for registration of mass spectra of intact and derivatized (permethylated) flavonoid glycosides. In some cases electron impact mass spectra of permethylated compounds were also recorded. Methylated samples after methanolysis and further derivatization of free hydroxyl groups (methylation or acetylation) were analyzed with gas chromatography-mass spectrometry. Combined information drawn from the registered mass spectra enabled us to define molecular mass, structure of aglycones and sugars, and positions of glycosidic bonds on the aglycon. Structures of four flavonoid monoglycosides were elucidated as follows: genistein 7-O-glucoside (1), genistein 4'-O-glucoside (2), 2'-hydroxygenistein 7-O-glucoside (3), and apigenin or genistein 8-C-glycoside (5). For the fourth O-glycoside (4) only molecular mass and masses of the aglycone and sugar were estimated.

18

Application of dynamic headspace technique and gas chromatography - mass spectrometry methods for bread

86%

Venskaityte A., Juodeikiene G., Hansen A., Petersen M. A.

Ekologia i Technika

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2005

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tom 13

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nr 5

19

Application of gas chromatography - mass spectrometry for the determination of volatile organic pollutants in plants

86%

Keymeulen R., Van Langenhove H.

Polish Journal of Environmental Studies

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1996

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tom 05

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nr 3

20

Measurement of angiotensin metabolites in organ bath and cell culture experiments by liquid chromatography - electrospray ionization - mass epectrometry (LC-ESI-MS)

86%

Bujak-Gizycka B., Madej J., Wolkow P. P., Olszanecki R., Drabik L., Rutowski J., Korbut R.

Journal of Physiology and Pharmacology

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2007

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tom 58

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nr 3

The metabolism of renin-angiotensin system (RAS) is more complicated than previously expected and understanding the biological phenomena regulated by variety of angiotensin metabolites requires their precise and possibly comprehensive quantitation. Physiological concentrations of angiotensins (Ang) in biological fluids are low, therefore their accurate measurements require very sensitive and specific analytical methods. In this study we developed an accurate and reproducible method of quantitation of angiotensin metabolites through coupling of liquid chromatography and electrospray ionization - mass spectrometry (LC-ESI-MS). With this method main angiotensin metabolites (Ang I, II, III, IV, 1-9, 1-7, 1-5) can be reliably measured in organ bath of rat tissues (aorta, renal artery, periaortal adipose tissue) and in medium of cultured endothelial cells (EA.hy926), exposed to Ang I for 15 minutes, in the absence or in the presence of angiotensin converting enzyme inhibitor, perindoprilat. Presented LC-ESI-MS method proved to be a quick and reliable solution to comprehensive analysis of angiotensin metabolism in biological samples.

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