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The effect of abscisic acid and dimethyl sulfoxide and different temperatures on the cryopreservation process of Abies nordmanniana (Steven) Spach embryogenic callus

100%

Nawrot-Chorabik K., Sitko K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

2

Efficient cryopreservation by droplet vitrification of pentaploid roses and the phenotype of regenerated plants

100%

Pawlowska B., Szewczyk-Taranek B.

Acta Societatis Botanicorum Poloniae

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2015

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tom 84

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nr 4

Shoot tips from in vitro plants of four rose species were cryopreserved by the droplet vitrification method. Optimized conditions involved exposure to loading solution for 20 min, then treatment with plant vitrification solution (PVS2) for 20 min (Rosa agrestis, R. canina and R. dumalis) or 30 min (R. rubiginosa) followed by freezing in liquid nitrogen. Survival rate ranged from 78.3 to 95.1%, depending on the species. Regrowth rate of shoot tips was 50.5% for R. agrestis, 63.2% for R. rubiginosa, 71.4% for R. dumalis and 78% for R. canina. The preculture of donor plants in a medium with 0.25 цЫ sucrose facilitated the isolation of shoot tips and increased regrowth rate after cryopreservation. Plant regeneration was carried out in Murashige and Skoog medium with 1 цЫ 6-benzylaminopurine, 1.5 цЫ gibberellic acid and 0.087 M sucrose. Plants regenerated from cryopreserved shoot tips did not display morphological alterations in comparison with non-cryopreserved shoot tip - derived plants.

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The influence of long-term storage in liquid nitrogen on survival and pathogenicity of Phytophthora infestans isolates

100%

Sobkowiak S., Zarzycka H., Sliwka J.

Journal of Plant Protection Research

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2012

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tom 52

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nr 4

A set of 14 aggressive Phythophthora infestans (Mont.) de Bary isolates with a wide range of virulence, was stored for eight years (2002-2010) in liquid nitrogen at -196°C (209 samples) and under paraffin oil at 7°C (70 test tubes). The survival rate of samples stored in liquid nitrogen was scored as 88%, 45 days after thawing. The revived isolates were passaged through potato tissues four times and tested for virulence on a set of 11 Black’s differentials. The mean number of virulence factors per isolate, of these P. infestans isolates evaluated before storage, was 7.4. The isolates stored in liquid nitrogen and under paraffin oil and passaged four times through potato tissues showed a similar mean number of virulence factors per isolate, 7.3 and 6.9, respectively. Isolates stored under paraffin oil showed no expression of factor avr5, but expression of this factor occurred in six isolates after liquid nitrogen treatment. The initial expression of this factor occurred in four isolates. Before storage, the average aggressiveness of the tested isolates was assessed as 1.7, on a 1-9 scale, where 1 means the most aggressive. After storage and after four subsequent passages through potato tissues, the mean aggressiveness of isolates stored in liquid nitrogen and under paraffin oil reached the level of 1.5 and 2.1, respectively. The mean aggressiveness was not significantly different from the level of initial assessment.

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Sensitivity of Tilia cordata seeds to dehydration and temperature of liquid nitrogen

100%

Chmielarz P.

Dendrobiology

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2002

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tom 47 Supplement

The aim of the study was to assess the susceptibility of small-leaved lime (Tilia cordata Mill.) seeds to drying and freezing in liquid nitrogen (-196°C). Seed samples were frozen in liquid nitrogen for 24 h at 11 different levels of seed moisture content (m.c.), ranging from 3.1% to 22.8% (fresh weight basis). All samples, including unfrozen control samples, were subjected to scarification with concentrated sulphuric acid (Tylkowski 1998) either before or after freezing. Seed pre-treatment before germination (at 3~15°C/16~8h) involved cold stratification at 3°C without substrate. Seed drying to 3.1% m.c. significantly reduced their germinability (to 63%), as compared to the high germinability (82-88%) of seeds with 5.2-20.9% m.c. Thus seeds of this species can be assigned to the ‘suborthodox' category. Such a high germinability (79-87%) was preserved after freezing in liquid nitrogen in samples dried to 9.0-17.4% m.c. if scarification was performed before freezing, and in samples dried to 9.1-16.2% m.c. if scarification was performed after freezing. The highest percentage of seedlings emerged after freezing in liquid nitrogen from seeds dried to 11.1-20.1% m.c. (emergence 65-75%) if scarification was performed before freezing, and from seeds dried to 7.3-17.8% m.c. (emergence 53%-71%) if scarification was performed after freezing.

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Effect of spawn storage at the temperature of liquid nitrogen of four strains of field mushroom Agaricus bisporus [Lange] Imbach on its growth and pinhead setting

100%

Sobieralski K., Salwin M.

Roczniki Akademii Rolniczej w Poznaniu. Ogrodnictwo

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2006

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tom 40

6

Employment of encapsulation-dehydration method for liquid nitrogen cryopreservation of ornamental plant explants propagated in vitro

80%

Pawlowska B.

Folia Horticulturae

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2008

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tom 20

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nr 1

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Effect of pre-freezing treatment methods on the quality retention of thawed areca fruit

80%

Zhang M., Duan Z., Zhang J., Peng J.

International Agrophysics

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2003

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tom 17

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nr 4

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Bank zasobow genowych ziemniaka in vitro w Polsce i na swiecie

71%

Sekrecka D., Lesinska M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2007

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tom 517

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nr 1

115-119

There are three strategies of potato genotype storage: as microtubers, in vitro plantlets under slow-growth conditions and shoot tips conserved in liquid nitrogen. Microtubers are obtained from cultures grown on the medium enriched with 80 g·dm⁻³ sucrose, and after harvest the storage period is counted down. The slow-growth is obtained by lowering the temperature (to 10ºC) and irradiance and by adding the growth inhibitors (phytohormones and osmoticum). For shoot tips storage, the droplet technique is used: the shoot-tips are placed on aluminium foil in a cryoprotectant droplet and then they are submerged in liquid nitrogen. In this work, all these techniques were discussed, especially the in vitro genotype bank in Bonin.

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Przechowywanie zasobów genetycznych czosnku pospolitego (Allium sativum L.) w ciekłym azocie

71%

Olas M., Kotlinska T.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2010

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tom 555

Do długoterminowego przechowywania zasobów genowych czosnku zastosowano metodę witryfikacji. Krioprezerwacji poddano obiekty czosnku tworzące i nietworzące pędów kwiatostanowych. Materiał badawczy stanowiły stożki wzrostu izolowane z cebulek powietrznych oraz roślin otrzymanych w kulturach in vitro. Stożki wzrostu wielkości 3-5 mm i średnicy ok. 2 mm traktowane były roztworem PVS3 (50% sacharoza, 50% glicerol), po czym bezpośrednio zamrażano w ciekłym azocie. Przeżywalność eksplantatów określano po 2 tygodniach od rozmrożenia, natomiast ostateczną regenerację po 10 tygodniach. Regeneracja eksplantatów uzyskanych z cebulek powietrznych wahała się od 24% do 83%, natomiast w przypadku stożków wzrostu izolowanych z roślin otrzymanych w kulturach in vitro od 0,23% do 33,3% i była uzależniona od genotypu.

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Porównanie metod przechowywania i konserwacji bakterii

71%

Sadowska K., Lukaszewska-Skrzypniak N., Rataj-Guranowska M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2010

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tom 555

Każda metoda konserwacji powinna przede wszystkim zapewniać maksymalną przeżywalność komórek, stabilność cech fizjologicznych i genetycznych, przeciwdziałać spontanicznym mutacjom oraz ograniczać przypadkowe zanieczyszczenia. Jednym z zadań kolekcji jest dobór skutecznych metod przechowywania drobnoustrojów w warunkach laboratoryjnych. Kolekcja Banku Patogenów i Badania ich Bioróżnorodności liczy obecnie 119 izolatów bakterii, należących do rodzajów Clavibacter, Pseudomonas, Xanthomonas, Chryseobacterium, Paenibacillus, Pectobacterium, Erwinia i Rhizobium. Szczepy bakteryjne przechowywane w kolekcji konserwowane są opcjonalnie trzema metodami: krioprezerwacji z zastosowaniem probówek Bacto-Protect (stosowana w BPR od 1997 roku), metodą liofilizacji (od 1999 roku) oraz mrożenia kultur w ciekłym azocie (od 2005 roku). Najnowszą i najlepszą metodą do przechowywania wszystkich gatunków bakterii i grzybów z zachowaniem ich patogeniczności jest zamrażanie kultur w ciekłym azocie. Wadą tej metody jest wysoki koszt aparatury i niebezpieczeństwo pracy z ciekłym azotem.

Ograniczanie wyników

The effect of abscisic acid and dimethyl sulfoxide and different temperatures on the cryopreservation process of Abies nordmanniana (Steven) Spach embryogenic callus

Efficient cryopreservation by droplet vitrification of pentaploid roses and the phenotype of regenerated plants

The influence of long-term storage in liquid nitrogen on survival and pathogenicity of Phytophthora infestans isolates

Sensitivity of Tilia cordata seeds to dehydration and temperature of liquid nitrogen

Effect of spawn storage at the temperature of liquid nitrogen of four strains of field mushroom Agaricus bisporus [Lange] Imbach on its growth and pinhead setting

Employment of encapsulation-dehydration method for liquid nitrogen cryopreservation of ornamental plant explants propagated in vitro

Effect of pre-freezing treatment methods on the quality retention of thawed areca fruit

Bank zasobow genowych ziemniaka in vitro w Polsce i na swiecie

Przechowywanie zasobów genetycznych czosnku pospolitego (Allium sativum L.) w ciekłym azocie

Porównanie metod przechowywania i konserwacji bakterii