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The effect of low potassium regimes on two lines, ILL 6796 (salt-tolcrant) and ILL 6439 (salt-sensitive), of lentil (Lens culinaris) was studied in a pot experiment under glasshouse conditions. Plants of the two lines were treated with 78, 39, 19.5, and 9.75 mg K·L⁻¹ in Rorison’s nutrient solution. ILL 6796 excelled ILL 6439 in biomass production under varying K regimes. Of the various organic solutes measured in the present study only total soluble sugars proved to be helpful in discriminating the lines, i.e., total sugars increased consistently in ILL 6796 with a decrease in K level of the growth medium, whereas in ILL 6439 they decreased significantly with a decrease in K levels. ILL 6796 showing high efficiency for K utilisation (high shoot growth) could be of great economic value in terms of its use in soils deficient in K.
Seed protein profiles were studied in 144 lentil accessions intensively collected from all over Pakistan. Heterogeneous populations were isolated on the basis of SDS-PAGE, and 13 polymorphic protein peptides were found, representing almost all the variation reported so far in lentil. The low diversity of accessions from the Northern Area and North Western Frontier Province, the most geographically diverse areas, suggested the need for more exploration so that the maximum genetic diversity of the areas can be truly represented. Clusters based on agro-ecological zones did not prove adequate for evaluation of lentil resources, whereas 63 of 108 accessions (58.3%) were grouped together by altitude and provincial distribution. The study confirmed the wealth of phenotypic divergence in the local lentil. A small sample of accessions from a particular region might not reflect the actual diversity within that region. Samples representing total diversity in particular countries or regions should be evaluated, so that a representative rather than a random set of accessions can be included in investigations of diversity on regional or continental scales. As Pakistan is in the vicinity of the centre of diversity of lentil, high variation of various parameters is expected, and that can be found only if a complete set of germplasm is studied.
Background. Lentil (Lens culinaris) is an important crop in many developing countries. Usefulness of protein isolates in the human nutrition and animal feeding have been also studied. Improvement of protein precipitation and fractionation efficiency by using different flocculants was the aim of this study. Material and methods. The polyelectrolytes Magnafloc LT-22 and Magnafloc LT-25 were tested in the process of coagulation and fractionation of protein from lentil protein extracts. Proteins were extracted from flour with 0.5 M NaCl in the 50 mM Tris-HCl buffer pH 7.5 and 2 mM NaOH at ratio 1:10. Protein were coagulated at different pH (6, 5, 4, 3) using flocculants in three different concentration 0.1, 0.3 and 0.5%. Samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results. Influence of extraction medium on the yield and quality of protein was visible. In all studied cases proteins were the most effective precipitated with Magnofloc LT-25. Application of different pH condition for coagulation caused fractionation of lentil protein. Gel electrophoresis of protein of all studied samples showed different molecular weight subunit patterns ranging from 8 to 102 kDa. Conclusions. Using of flocculants as coagulating factors allows obtaining a high concentrated protein isolates, however the yields of flocculation were determined by different extraction systems, concentration of flocculants and pH condition of process
Background. The lentil plant, Lens culinaris L., is a member of the Leguminoceae family and constitutes one of the most important traditional dietary components. The purpose of the current study was to investigate the effects of sprouting for 3, 4, 5 and 6 days on proximate, bioactive compounds and antioxidative characteristics of lentil (Lens culinaris) sprouts. Material and methods. Lentil seeds were soaked in distilled water (1:10, w/v) for 12 h at room temperature (~25°C), then kept between thick layers of cotton cloth and allowed to germinate in the dark for 3, 4, 5 and 6 days. The nutritional composition, protein solubility, free amino acids, antinutritional factors, bioactive compounds and antioxidant activity of raw and germinated samples were determined using standard official procedures. Results. Sprouting process caused significant (P < 0.05) increases in moisture, protein, ash, crude fiber, protein solubility, free amino acids, total, reducing and nonreducing sugars. However, oil content, antinutritional factors (tannins and phytic acid) significantly (P < 0.05) decreased. Results indicated that total essential amino acids of lentil seeds protein formed 38.10% of the total amino acid content. Sulfur-containing amino acids were the first limiting amino acid, while threonine was the second limiting amino acid in raw and germinated lentil seeds. Sprouting process has a positive effect on the essential amino acid contents and protein efficiency ratio (PER) of lentil sprouts. Phenolics content increased from 1341.13 mg/100 g DW in raw lentil seeds to 1411.50, 1463.00, 1630.20 and 1510.10 in those samples germinated for 3, 4, 5 and 6 days, respectively. Sprouted seeds had higher DPPH radical scavenging and reducing power activities. Conclusions. Based on these results, sprouting process is recommended to increase nutritive value, and antioxidant activity of lentil seeds.
Density and diversity of Fusarium species and other fungi associated with rhizosphere and rhizoplane of lentil and sesame plants at three different growth stages were investigated. Sixteen species of Fusarium were isolated from rhizosphere (13 species) and rhizoplane (11) of both plants studied. In lentil, 11 species were recorded from its rhizosphere (9 species) and rhizoplane (8). Fusarium species associated with lentil rhizoplane gave highest number of propagules at the first stage of plant growth while the ones of Fusarium associated with the rhizosphere produced the highest number at the second stage of growth. F. solani was the most common in the three growth stages. In addition, of two growth stages, F. culmorum and F. tricinctum were isolated from the rhizosphere while F. nygamai and F. verticillioides from the rhizoplane. The other species were recorded from only one growth stage of lentil plant. In sesame plants, rhizosphere yielded nine Fusarium species while rhizoplane gave only six from the three stages investigated. Stage I of sesame rhizosphere possessed the highest colony forming units of Fusarium. As the case for lentil, F. solani was the most common species in sesame rhizospere and rhizoplane. F. verticillioides and F. nygamai (in three different growth stages) followed by F. oxysporum and F. tricinctum (in two growth stages) were recorded using the dilution-plate and/or soil-plate methods from sesame rhizosphere soils. Rhizoplane Fusarium species of sesame plants were isolated at the three different growth stages with almost equal number of colony forming units. F. poae came after F. solani in its frequency since it was recovered from two growth stages. Several of the isolated species are well-known as pathogens to many cultivated plants. To the best of our knowledge, three species are recorded here for the first time in Egypt from the rhizosphere (F. acutatum), rhizoplane of sesame plants (F. longipes) and from rhizosphere of both lentil and sesame and rhizoplane of lentil (F. nygamai).
Seed priming is one of the potential physiological approaches to enhance the seed germination under the salinity stress. The present study examined the role of two seed priming molecules: salicylic acid (SA) and hydrogen peroxide (H2O2), in enhancing the salt tolerance of lentil seeds at germination stage. Salinity stress caused significant decrease in germination percentage and primary root elongation. This decrease was associated with significant increase in lipid peroxidation and total lipid (TL) contents in embryonic axis. The catalase (CAT), guaiacol peroxydase (GPOX) and superoxide dismutase (SOD) activities remained unchanged or decreased significantly under the influence of salt stress, in both embryonic axis and cotyledons. Starch mobilization was not affected by the salt stress. The two priming treatments effectively alleviated the negative effects of salinity stress. SA and H2O2 applications after dose optimization resulted in a significant enhancement of germination percentage and primary root elongation. No significant changes in starch, soluble sugars contents and SOD activity were detected following SA and H2O2 treatments. Seed priming treatments triggered the activities of GPOX and CAT and caused the reduction in lipid peroxidation, especially in embryonic axis. TL content and especially the fatty acid C18:3 increased after SA applications. Better performance under salt stress of primed lentil seeds was associated with lower lipid peroxidation, and activation of enzymatic antioxidative defense system. Obtained results confirm the potential for using SA and H2O2 to improve germination and plant growth under salt stress conditions.
In mutation breeding, mutagenic effectiveness and efficiency are the base parameters to predict the mutagenic potency of any mutagen. Studies on mutagenic effectiveness and mutagenic efficiency of physical mutagen (gamma rays) and chemical mutagen (hydrazine hydrates; HZ) on two cultivars of lentil (Lens culinaris Medik.), viz. DPL 62 (macrosperma) and Pant L 406 (microsperma) have been reported. Dry and healthy seeds were treated with four doses of each gamma rays (100-400 Gy), HZ (0.1-0.4 %) and their combinations. Frequencies of the induced agro-morphological variations into different phenotypic categories were estimated in M2 population that resulted into identification and isolation of wide range of mutants with altered phenotypes. Data on effectiveness and efficiency of various mutagenic treatments calculated on the basis of the frequency of chlorophyll mutations, which showed effectiveness and efficiency were higher at the moderate doses of gamma rays and HZ, while in case of combination treatments; lower doses were most effective and efficient with few inter-varietal exceptions. Phenotyping of the mutants revealed that growth habits was the most sensitive category to which most of the mutant belongs, followed by leaf and flower/pod/seed in both the cultivars studied. Overall, the screened and isolated mutants with economically important agronomic traits can be further propagated in the subsequent generation for development of elite lentil mutant cultivars.
The effect of processing conditions (soaking in water for 0-3 h, cooking in water or 1-3% salt solution for 15 or 30 min) was tested. The water absorption was affected both by soaking and cooking, but the salt content had little effect. The descriptors connected with visual testing and those connected with texture were influenced more by cooking, than by soaking; relationships between different characteristics were either linear or logarithmic or hyperbolic. They were related to the water content in cooked lentils, which had, however, no significant effect on the flavour acceptance. It rose with the intensity of salty flavour till a flat optimum at medium saltiness of 2% salt in the cooking liquid.
Lentil meal proteins were treated by chymotrypsin. Hydrolysis was controlled with the pH-stat method. Degree of hydrolysis (DH) was evaluated and after 120 min of the process amounted to 13%. SDS-PAGE and SE-HPLC methods were used to study molecular weight distribution of lentil meal proteins and their chymotryptic hydrolysates of DH 2%, 4%, 8% and 12%. Bands in the range of 21–66 kDa were predominant on the electrophoregram of lentil proteins. Chymotrypsin treatment resulted in releasing the hydrolysis products of both high molecular weight (62,000; 30,500 Da) molecules and small peptides (<6,500 Da). At the first stage of hydrolysis (DH 2.0%) intermediate products are formed, which are then further hydrolysed. Chromatographic separation confirmed the results of SDS-PAGE. Larger polypeptides and unhydrolysed proteins are present in hydrolysate of DH 12% but products of hydrolysis with molecular weight of 0.5–6.5 kDa are predominant. No simple correlation between degree of hydrolysis and intensity of bitterness and astringency sensation was noticed. Bitterness of hydrolysates was not high (2.25-2.35 a.u.).
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Metoda oceny podatnosci strakow soczewicy na pekanie

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Opracowano sposób oceny podatności strąków soczewicy na pękanie, polegający na wyznaczeniu maksymalnej siły oraz energii potrzebnej do otwarcia strąka. Wykorzystując aparaturę wytrzymałościową Instron rozdzielano strąk na dwie łupiny przez, rozciąganie. Materiałem badawczym były polskie odmiany Tina i Anita. Uzyskane wyniki z pomiarów laboratoryjnych porównano z samoosypywaniem się nasion na poletkach w czasic dojrzewania roślin.
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